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Medical Laboratory Technology Journal
Published by Poltekkes Kemenkes Banjarmasin
ISSN : -     EISSN : 24610879     DOI : https://doi.org/10.31964/mltj
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 193 Documents
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The Effect of Methanol Fractıonated of Luvunga sarmentosa on In Vıtro Sperm Membrane Integrıty Permatasari, Silvani; Nuha, Alya Firyal; Ratnasari, Arini; Widayati, Ratna; Ka Praja, Rian
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.571

Abstract

Infertility cases in the world are increasing in 2020, with 186 million individuals affected worldwide. The most common cause of male infertility is due to decreased sperm quality. Sperm membrane integrity is known to play an essential role in the fertilization ability of spermatozoa. The methanol fraction of Luvunga sarmentosa root extract has the highest antioxidant activity compared to other fractions and can improve sperm motility in vitro. This study aims to determine the effect of the methanol fraction of Luvunga sarmentosa root extract on human sperm membrane integrity and the effective concentration of Luvunga sarmentosa methanol extract fraction to improve sperm membrane integrity in vitro. The samples used were washed human spermatozoa in vitro, then added BWW medium, and incubated at 37℃ for 1 hour with methanol fraction of Luvunga sarmentosa root extract at concentrations of 100, 500, 1000, and 5000ng/ml as well as the control group (BWW only). Furthermore, 100uL of each sample was added to 1mL of Hypo-osmotic swelling (HOS) solution, then incubated at 37oC for 30 minutes to see the integrity of the spermatozoa membrane from the tail swelling. The results showed that sperm membrane integrity significantly increased at 100, 500, 1000, and 5000ng/mL concentrations compared to the control group. The methanol fraction of Luvunga sarmentosa root extract was most effective at a concentration of 100ng/mL. Methanol fraction of Luvunga sarmentosa root extract can improve human sperm membrane integrity.
Increased levels of IL-4 in the Spleen of BALB/c Mice after 65.5 kDa Pili Protein Klebsiella pneumoniae Immunization Putra Uta, Diego Rafi; Mufida, Diana Chusna; Sutejo, Ika Rahmawati; Agustina, Dini; Suswati, Enny; Shodikin, Muhammad Ali
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.575

Abstract

Pneumonia is an infection of the lung parenchyma caused by Klebsiella pneumoniae, resulting in a high mortality rate of millions each year. To reduce these deaths, one potential solution is to create a vaccine that utilizes virulence factors of this microorganism, such as pili. During the early phase of infection, these antigens have a crucial role and can stimulate the production of memory b cells. These cells are activated by IL-4 (interleukin-4) in lymphoid organs, such as the spleen. Pathogen exposure such as virulence factors can stimulate the secretion of IL-4 in the spleen organ. This study aims to investigate the role of Klebsiella pneumoniae pili in this process. The research conducted from May to December 2023 at the Microbiology Laboratory, Faculty of Medicine, University of Jember was purely experimental, using Balb/c mice with IL-4 levels in the spleen organ as variables. The study used 15 mice, divided into control (K1), adjuvant (K2), and antigen (K3) groups. The research data were analyzed using non-parametric tests, specifically the Kruskal-Wallis and Post Hoc tests. The Kruskal-Wallis test revealed significant differences (p=0.003). In the Post Hoc test, a significant difference was found between the control and antigen groups (p=0.002). The results conclusively demonstrate that induction of Klebsiella pneumoniae pili protein 65.5 kDa significantly increases IL-4 levels in the spleen. Future studies should consider adding serum specimens to provide additional information.
The Impact of Phaleria macrocarpa Fruit Flavonoid Extract on Endometrial Thickness in Mice Menopausal Model Wijaya, Dyah Ayu Septika; Dwi Wulandari, Recavery; Sutrisno, Sutrisno; Irwanto, Yahya; Anita, Kenty Wantri
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.584

Abstract

This study aims to determine how the effect of flavonoid phaleria macrocarpa fruit extract on the endometrial thickness of Mus musculus which is used as an experimental animal with a menopause model. This study employed a randomized post-test-only control group design on 32 female mice. Ovariectomy simulated menopause, followed by treatment with flavonoid from Phaleria macrocarpa fruit extract. Post-observariectomized, follicle-stimulating hormone levels indicated menopausal status. Hematoxylin-Eosin methods assessed endometrial thickness. The result indicated reduced endometrial thinning in the positive control group (K+). Conversely, the treatment group (P1-P4) exhibited increased endometrial thickness post-flavonoid extract administration. Ovariectomized-induced estrogen decline resulted in reduced endometrial thinning, mimicking menopausal conditions. Statistically, flavonoid extract administration increased endometrial thickness in ovariectomized-induced menopausal mice, with optimal effect observed at 7.5 mg/mice/day for 14 days. The preferred intervention dose for altering endometrial thickness in menopausal conditions was identified as P1 (3.75 mg/mice/day). In conclusion, a flavonoid from Phaleria macrocarpa fruit extract mitigates endometrial thinning in the menopausal mice model. For implication in menopausal women, clinical trials need to be conducted to evaluate the dosage of these flavonoids for their potential as a natural SERM (Selective Estrogen Receptor Modulator) before clinical application in managing endometrial health. Additionally, research on molecular effects particularly regarding ER (Estrogen Receptors) in the endometrial organ, is needed to confirm their phytoestrogenic effect.
Negative Effect of Cigarette Smoke: Black Garlic Opportunities for Prevention of Ovulation Disorders Asih, Sari Wulan; Nurseta, Tatit; Handono, Kusworini; Yudhanto, Hendy Setyo
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.585

Abstract

Women's issues are primarily related to infertility. This study aimed to demonstrate that ovarian healing in wistar rats exposed to cigarette smoke was impacted by the administration of extracted black garlic. This research uses True Experimental methods in vivo in the laboratory to identify the cause and effect of the variables being tested. This method involves replication, randomization, and control. The design chosen was a post-test-only control group design, where the experimental group received treatment and the control group did not, without random selection. The results of the study showed that there was no effect due to exposure to cigarette smoke with an increase in cortisol and a decrease in the number of secondary follicles in the ovaries of female wistar rats as well as the administration of Black Garlic (Allium sativum) extract. Black garlic extract unaffected cortisol levels and the number of secondary follicles or repair of the ovaries due to exposure to cigarette smoke. Further research is needed in dosing black garlic extract or combining it with other ingredients to provide effective results.
Extract Ethanol of Tempuyung (Sonchus arvensis) Leaves as Anti-Hyperuricemia: In Vitro Studies Parisa, Nita; Hidayat, Rachmat; Fatmawati, Fatmawati
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.594

Abstract

Hyperuricemia is a condition where there is an increase in blood uric acid levels above 7 mg/dL in men and 6 mg/dl in women. One of the enzymes that plays a role in hyperuricemia is xanthine oxidase which can inhibit uric acid synthesis. Tempuyung leaves (Sonchus avernis) are one of the plants that have antihyperuricemia properties. This study aims to determine the effect of ethanol extract of tempuyung leaves on xanthine oxidase in vitro. Tempuyung leaves come from Palembang, South Sumatra, Indonesia, and are macerated with 96% ethanol until a thick extract is obtained. The xanthine oxidase inhibition test was carried out on tempuyung leaf extract and Allopurinol with respective concentrations of 6.25; 12.5; 25; and 50 ppm and followed by absorption measurements using UV-V spectrophotometry at a wavelength of 293 nm and determining the IC50 value. The research results showed that the ethanol extract of daun tempuyung leaves had an IC50 value of 23.37 ppm, higher than allopurinol of 17.16 ppm which was used as a reference. These results classify the ethanol extract of daun tempuyung leaves as a strong inhibitor of the xanthin oxidase enzyme. Therefore, the ethanol extract of daun tempuyung leaves has the potential to be anti hyperuricemic.
The Efficacy of Green Grapefruit (Vitis Vinifera L) Extracts on Reducing Blood Glucose in a Diabetic Rat Model Habibah, Nur; Rofi'ah, Lisa Khofidatur; Bekti, Heri Setiyo; Dewi, Ni Nyoman Astika; Wilankrisna, Luh Ade; Posmaningsih, Dewa Ayu Agustini
Medical Laboratory Technology Journal Vol. 10 No. 2 (2024): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i2.606

Abstract

Diabetes mellitus (DM) is a chronic disease in which the pancreas cannot produce enough insulin hormone or when the body cannot use the insulin hormones properly. Green grapes contain flavonoids, anthocyanins, tannins, phenolic acids, and resveratrol and are high in antioxidants that are beneficial in lowering blood glucose levels. This study aims to determine the effectiveness of green grapefruit (Vitis vinifera L) extract in reducing blood glucose levels in a diabetic rat model. This research is a true experiment with a post-test-only control group design, using 24 male Wistar white rats aged 8-12 weeks and weighing 130-200 grams. The rats were divided into three groups: the positive control (I), green grapefruit extract (II), and normal group (III). The results showed that green grapefruit extract effectively reduced blood glucose levels in experimental animals by a percentage of 98.4%. The Independent T-Test showed a value of ρ>0.05 (0.533), so there was no significant difference in glucose levels between green grapefruit extract and positive control. This study concludes that green grapefruit extract was effective at 98.4% in reducing blood glucose levels in the diabetic rat model, compared to the positive control. The promising results of this study need to be continued by establishing a standardized optimal dose, assessing long-term effects, and implementing clinical trials to evaluate efficacy and safety.
Analysis of COVID-19 Antibodies in Patients Who Have Been Vaccinated With Non-Vaccinated Patients with Confirmed COVID-19 Life Hutabarat, Mustika Sari H; Lubis, Agnes Felicia; Sinaga, Hotman
Medical Laboratory Technology Journal Vol. 10 No. 2 (2024): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i2.607

Abstract

COVID-19 immunization is a government initiative to prevent transmission and reduce the incidence of disease and death associated with the SARS-CoV-2 virus. Although the SARS-CoV-2 virus is no longer a significant threat, older adults with comorbidities or people who are immunosuppressed should remain vigilant by maintaining a distance of at least one meter from others, covering their mouths with their elbows when coughing or sneezing, washing their hands regularly, and wearing masks. However, many groups oppose vaccination for various reasons, including the perception that the COVID-19 vaccine is less effective and efficient in preventing SARS-CoV-2 virus infection. This study compared COVID-19 antibody titers in participants who had arrived, without mentioning the vaccine brand, with those who had not yet arrived but had a history of SARS-CoV-2 infection. This study design used a cross-sectional study; the number of samples in the study was 34, the location of this study was the Palembang Public Health Laboratory Center, and the statistical test in the study used the Independent T-test. The study results indicate that the mean antibody titer in the vaccinated group was 98.123. In contrast, in the unvaccinated group, it was 70.641, demonstrating a statistically significant difference with a p-value of 0.000. Further research suggests whether there is a relationship between antibody titers in subjects who have been vaccinated for the second and third time with a history of exposure to the COVID-19 virus.
The Analgesic Effect and Toxicity of Red and White Ginger on Mus Musculus with the Acetate Writhing Test Dewi, Vonny Khresna; Barkinah, Tut; Kirana, Rita; Rizki, Muhammad Ikhwan
Medical Laboratory Technology Journal Vol. 10 No. 2 (2024): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i2.611

Abstract

Based on data from the World Health Organization in 2020, the incidence of dysmenorrhea was 1,769,425 (90%) women who suffered from dysmenorrhea, with 10-16% suffering from severe dysmenorrhea. The problem of pain is very disruptive to activities, so prevention and treatment of pain must be a concern. Ginger is a natural ingredient that grows in South Kalimantan and is empirically used to treat pain. The study aimed to determine the analgesic effects and toxicity of red and white ginger from Banjarmasin and Banjar Regency. The nutrient content profile of the soil where the ginger grew was analyzed, and it was determined by nitrogen content, cation exchange capacity, and soil pH. The content of active compound groups was analyzed using specific reagents to determine the presence of active compounds. Determination of total phenolic levels using a colorimetric method using a UV-Vis spectrophotometer. The instant ginger formula was optimized with organoleptic test result parameters. Acute toxicity tests using male Wistar rats with observations for 14 days. Analgesic tests were conducted on Mus musculus using the acetic acid writhing test. The results showed that the highest nitrogen (1,34%), cation exchange capacity (29.22 me/100 g), and soil pH levels (6,89) were in the soil where the ginger samples from Banjar Regency grew. The chemical compound content in all gingers contained the same phenolics, flavonoids, tannins, saponins, and terpenoids. The levels of marker compounds in succession from Banjarmasin red ginger, Banjarmasin white ginger, Banjar Regency red ginger, and Banjar Regency white ginger were 7.43%, 6.26%, 8.52%, and 7.12%, respectively. The results of formula optimization showed that all gingers had a sweet and spicy taste, a distinctive odor, powder form, and a reddish-brown color in red ginger and pale yellow in white ginger. The toxicity test results showed that at doses of 300 mg/Kg BW and 2000 mg/Kg BW, there was no toxic effect on the administration of ginger extract. Analgesic activity showed that all gingers had an analgesic effect at a dose of 200 mg/Kg BW. The analgesic effects from the strongest to the lowest were red ginger from Banjar Regency, red ginger from Banjarmasin, white ginger from Banjar Regency, and white ginger from Banjarmasin. This study concludes that red ginger from Banjar Regency has the most potent analgesic activity and no toxicity for all ginger.
The Relationship of Plasmodium sp Density to Platelet Count and Hemoglobin Levels in Malaria Patients at Bhayangkara Hospital TK I Pusdokkes Police Kramat Jati, Indonesia Inderiati, Dewi; Oktapıa, Nısa; Nida, Wadhatun; Zuraida, Zuraida; Hariutami, Desi
Medical Laboratory Technology Journal Vol. 10 No. 2 (2024): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i2.613

Abstract

Malaria is an acute and chronic disease. Globally, as many as 247 million positive cases of malaria were reported in 84 malaria-endemic countries. Indonesia is a malaria-endemic country with a total of 443,530 cases with a malaria prevalence of 89% reported from Papua province. This study examined the correlation between Plasmodium sp density platelet count and hemoglobin levels. The type of research used in this study was observational analytics with a cross-sectional study approach design. The sample and population in this study were malaria-positive patients who had their platelet count and hemoglobin levels checked as recorded in the report from Bhayangkara Hospital Tk I Pusdokkes Polri Kramat Jati, Indonesia, namely using medical record data from months January to December a total of 54 samples with data analysis in this study using descriptive univariate analysis and bivariate analysis using the Fisher Exact test. This study showed that the density of Plasmodium sp with the number of platelets had a significant result, namely 0.088, which indicates that the density of Plasmodium sp does not affect the platelet count. In contrast, the density of Plasmodium sp with hemoglobin levels had a significant result, namely 0.023, which indicated a lower Hb level of malaria sufferers. The higher the level of Plasmodium density.
The Potential of Probiotics from West Sumatra, Indonesia on Low-Density Lipoprotein and Interleukin-6 Levels with Dyslipidemia Ridwan, Rhinie Uphita; Putranto, Wachid; Nuhriwangsa, Adi Magna Patriadi
Medical Laboratory Technology Journal Vol. 10 No. 2 (2024): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i2.618

Abstract

Dyslipidemia is a lipid metabolism disorder characterized by abnormal elevations or reductions in one or more lipid fractions within the plasma. This condition is a critical component of both metabolic syndrome and cardiovascular disease (CVD). This study aims to see the potential of curd administration on Low-Density Lipoprotein (LDL) and Interleukin-6 (IL-6) levels in dyslipidemia patients. This study is an experimental study with a pre-post test control group design consisting of a control group and a treatment group totaling 42 people taken randomly at the DR Drs M Hatta Bukittinggi Brain Hospital in June and July 2024. In the three groups, LDL and IL-6 examinations were carried out and measured before and after curd for 14 days. Treatment group 1 was given curd as much as 150 grams, treatment group 2 was given curd as much as 200 grams, given 1x a day, and the control group was not given curd. Data analysis was performed using the Wilcoxon statistical test and paired t-test. Results: There was no significant difference in LDL (p=0.360) and IL-6 (p=0.932) levels after giving curd for 14 days. However, there was a decrease in LDL and IL-6 levels after giving curd for 14 days. Conclusion: The results showed that giving curd for 14 days in the treatment group 1 (150gram) and treatment 2 (200gram) can reduce LDL and IL-6 levels. Consuming probiotics for 14 days can reduce LDL and IL-6 levels.

Page 17 of 20 | Total Record : 193

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