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Articles 482 Documents
In Vitro Pancreatic Lipase Inhibitor Activity of Mangifera foetida Leaves Extract Oktira Roka Aji; Riris Asyhar Hudaya; Diah Asta Putri
Jurnal Biodjati Vol 6, No 1 (2021): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i1.10646

Abstract

Obesity is a condition where there is excessive fat in the body. Weight loss can be accomplished by inhibiting fat absorption through the inhibition of pancreatic lipase activity, a key enzyme in fat metabolism. Natural products from plants contain various phytochemical compounds that can act as pancreatic lipase inhibitors. This research was conducted to determine the lipase inhibitor activity of the methanol extract of Mangifera foetida leaves. Mangifera foetida leaves were extracted and then the total phenolic and flavonoid levels were determined. The extract was tested for its ability to inhibit pancreatic lipase in vitro. The inhibitory activities of the extract and Orlistat were measured using p-nitrophenyl palmitate as a substrate at concentrations of 31.25, 62.5, 125, 250, and 500 µg/mL. The results showed that the extracts of young leaves (YL) and mature leaves (ML) contained phenol and flavonoid compounds. The IC50 values of young leaf extract (YL) and mature leaves (ML) were 45.22 and 35.50 µg/ml, respectively. In conclusion, the Mangifera foetida leaf extract can be promoted as a good source of anti-obesity compounds
Organic Waste as Fertilizer to Increase the Number of Peanuts (Arachis hypogaea L.) Flowers Enceng Sobari; Ferdi Fathurohman
Jurnal Biodjati Vol 6, No 1 (2021): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i1.6538

Abstract

Peanut has high economic value and a significant role in food needs. the national food supply needs for peanuts to date have not been sufficient. This study aimed to determine the effect of organic waste which in this case is mushroom baglog compost and sheep manure on the flowers and pods of peanuts. The study was conducted on March-July 2018 in Subang district using the simple experimental plot method and group-randomized factorial design with two factors with each treatment done triplicate. The first factor is the baglog compost (soil without baglog compost, 90 g/plants, 120 gr/plants); the second factor is sheep manure dose (without sheep fertilizer, 90 gr/ plants, 120 gr/plants). The results showed that the application of baglog compost and sheep manure fertilizer at concentrations of 90 g and 120 g, respectively increased the number of flowers and potential pods. Moreover, Sheep manure fertilizer at a concentration of  120 g/plant, increased the dry weight of pods, seed, and 100 seeds even though was not significant.
Influence of Mates Virginity on Black Soldier Fly, Hermetia illucens L. (Diptera: stratiomyidae) Mating Performance Agus Dana Permana; Lulu Lusianti Fitri; Ucu Julita
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.9049

Abstract

   Hermetia illucens (L.), the black soldier fly (BSF), has raised attention due to its potential in solving various organic waste prob-lems and the benefits of the prepupa biomass as an alternative highly nutritious livestock feed. The availability of BSF populations strongly depends on mating success and reproduction. The mechanism of sex-ual selection during the mating period also determines the success rate of mating and reproduction and the survivorship of the offspring. Here, we analyzed how the influence of different mating status (virgin-ity) of mating pairs on mating success, daily oviposition, the number of eggs and fertility of eggs. BSF reared in semi-outdoor screen cages with five replication and four treatment of mates virginity combina-tion. An analysis of variance (ANOVA) was used to assess differences in mating and reproductive performance among treatment. Male and female BSF performed the remating activity. The virginity of males and females significantly influenced mating and oviposition frequen-cy. Mate choice was influenced by the virginity of mates. However, vir-ginity status of mates did not affect the number of eggs, eggs weight, and eggs fertility. Understanding of mate selection behavior in rela-tion to virginity in BSF served as important information to obtain the sustain population in the various scale of rearing design applicat 
Bacterial Contamination at Whiteleg Shrimp (Litopeanaeus vannamei) in Aquaculture Mashuri Masri -; Eka Sukmawaty; Fatmawati Nur; Suriani Suriani
Jurnal Biodjati Vol 6, No 1 (2021): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i1.11812

Abstract

Indonesia has a very high biodiversity, which has later become one of the natural products of interest to the international community, including fishery products. One of the high-demand Indonesian fishery products is whiteleg shrimp Litopeaneus vannamei. However, safety food Exported whiteleg shrimp products must meet the criteria, including free from bacterial contamination such as Escherichia coli, Salmonella, Vibrio cholera. This study attemptted to analyze E. coli, Salmonella, V. cholerae contamination in 3 ponds in Bojo, Cilellang, and Palanro Village in District Malusetasi, Barru Regency, South Sulawesi. Two samplings for each pond were conducted in the morning were pond water and  fresh whiteleg shrimp. SNI 2728-2018 specifies the quality and safety requirements for fresh shrimp. This standard applies to whole or headless fresh shrimp handled from fresh shrimp and does not apply to fresh shrimp that has undergone further processing. Based on SNI 2728-2018, the E. coli test showed positive in Cilellang Village (sample A) with 11 MPN/g, negative in Palanro Village (sample B) and in Bojo village (sample C) with the value of <2 MPN/g. Escherichia coli test showed positive in sample D (Vannamei shrimp in Cilellang Village) and sample E (Vannamei shrimp in Palanro Village) with 2.0 MPN/g, 17 MPN/g, respectively. Only sample F (Vannamei shrimp in Bojo village ) showed a negative result. As for the Salmonella test, positive results showed in sample A, while sample B and sample C showed negative results. The Vibrio cholerae test showed negative at all samples. . This study concludes that Whiteleg shrimp from ponds in Mallusetasi District is classified as safe for consumption.
Habitat and Distribution Modeling of Prehistoric Hippos (Hippopotamus sivalensis spp.) During Pleistocene in Java Island Andriwibowo Andriwibowo; Adi Basukriadi; Erwin Nurdin
Jurnal Biodjati Vol 6, No 1 (2021): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i1.10250

Abstract

Currently, there are only 2 extant species of hippos including common (Hippopotamus amphibius) and pygmy hippos (Choeropsis liberiensis) . But in prehistoric times, there were several species. During  Pleistocene these species were known to migrate to Java Island from Asian Continent and the species was Hippopotamus sivalensis spp. In this regard, this study aimed to model the habitat of H. sivalensis spp., ecology, and biodiversity of Hippopotamus sivalensis spp. based on the fossil record. The model was developed based on the Principal Component Analysis (PCA) method using the R statistical package. The results showed that there were 7 populations of H. sivalensis spp. that lived at various altitudes with an average of 177 m above sea level (95% CI : 123-232 m). According to PCA, there were at least 2 separate populations of H. sivalensis spp. One population occupies the forest while another occupies a habitat close to the coast. Currently the habitat for H. sivalensis spp. already changed. Based on habitat modeling, H. sivalensis spp. inhabit streams with submerged aquatic plants and shrubs and trees growing along river banks.
Study on Sex Determination and Impact of Sex Ratio to Reproduction Success in Black Soldier Fly Ramadhani Eka Putra; Nindia Safa&#039;at
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.9472

Abstract

Black soldier fly (BSF) (Hermetia illucens) is an insect spe-cies utilized as the converter of organic wastes into biomass applicable as a potential replacement of protein and lipid sources of animal feed and other industrial use. The limiting factor for the sus-tainability of this activity is the number of eggs produced as all processes started from the egg. Variable of quality of the substrate eaten during the larval stage and the proportion of males and fe-males are among the decisive factors for egg production. The pur-poses of this study were to understand the impact of the substrate to the production of a particular sex, to developed a simple method by comparing the weight and size of the pupae to distinguished between male and female, and to find the best sex ratio to produce the highest number of eggs. In this study, two types of substrates (balance proportion of macronutrient and high in protein and lipid were applied as feeding material for larvae. The weight and length of each pupa produced were measured and the pupae kept inside separated containers to find the morphological differences between male and female pupae. Adult insects produced from pupae then used for study on the effect of sex ratio on reproduction success. The adults kept inside 1 x 1 x 1 m screen cages with 3 groups of sex ratio (male : female) which were 50%:50% (P1), 60%:40% (P2), and 40%:60% (P3). The probability of sex was analyzed by logistic regression while the number of eggs estimated by the weight of the egg batch. The result showed substrate used in this study is not affect the sex ratio of adult flies, weight of pupae as the best predictor for sex of the adult flies, and the best ratio of male and female for egg production was 40:60. 
Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus Reni Indriani; Erma Prihastanti; Rini Budihastuti; Yulita Nurchayati
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5840

Abstract

Tomatoes (Lycopersicon esculentum Mill.) are a source of carotenoids they are easy to find. These compounds function as precursors of vitamin A, antioxidant, and prevent cancer. The extraction of carotenoid compounds for commercial products usually uses fresh plants, which are less efficient and require a lot of raw materials. The supply of these raw materials can be done through tissue culture. The frequency of subculture or supply of nutrients in tissue culture is very influential on the content of callus carotenoids produced. This study aimed to determine the effect of subculture frequency on growth, development and callus carotenoid content and to find out the right frequency of subculture to produce callus with optimal growth, development and carotenoid content. The design this study was a single Completely Randomized Design (CRD) with 4 treatments of subculture frequency and 5 replications. The data obtained were analyzed by ANOVA at the 95% test level followed by DMRT in case a significant different was found The results showed subculture frequency affected growth, development and carotenoid content of callus Lycopersicon esculentum. Mill. The most optimal treatment to induce growth and production of carotenoids in this study was treatment of thrice subculture while the most optimal treatment in inducing development was the twice subculture treatment.
Morphological characteristic and Phenetic Relationship of Lysurus periphragmoides Collected from West Java Rudy Hermawan; Laifa Fusvita; Naufal Hafizh Nugraha; Mega Putri Amelya
Jurnal Biodjati Vol 6, No 1 (2021): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i1.10724

Abstract

Lysurus is one of the unique genera in Basidiomycetes. It has a stinky odor and slime on the head. The unusual-shaped makes the species in Lysurus easily to be identified. One of Lysurus had been found in West Java, Indonesia namely L. periphragmoides. The specimen was deposited into Herbarium Bogoriense with code BO 24418. This study aimed to obtain specimens and characterize the Lysurus BO 24418 using morphological characteristics, and analyze the phenetic relationship among Lysurus species. Lysurus BO 24418 has two phases (egg and mushroom). The egg phase is usually hypogeous underground. The mushroom has a head and stem. The head bears the mature spore with slime distribute malodor. The stem has a hollow and spongy texture with a yellowish color. The numerical data of morphological characters of species in Lysurus were analyzed using NTSys ver 2.1 software. Ten characters were used to build a dendrogram using Sequential Agglomerative Hierarchical Nested (SAHN) cluster analysis with Unweighted Pair Group Method with Arithmetic Mean (UPGMA). Phallus indusiatus was selected as an outgroup. The analyses showed the specimen was classified as L. periphragmoides with 100% of similarity coefficient and it was close L. gardneri with 40,4% of similarity coefficient. The characters that cluster among them are stem surface, head type, egg diameter, and spore max length
Preliminary Study on Antibacterial Activity of Sawo Kecik (Manilkara kauki (L.) Dubard) Roots Extract Novida Pratiwi; Rini Retnosari; Sitoresmi Prabaningtyas
Jurnal Biodjati Vol 6, No 1 (2021): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i1.7708

Abstract

The roots of sawo kecik (Manilkara kauki (L.) Dubard) contain astringent that can be used to treat diarrhea for infants. However, the active antibacterial substance in sawo kecik roots has not been known. Therefore, the research to understand the antimicrobial activity of sawo kecik roots extract against Escherichia coli and Staphylococcus aureus was performed. Sawo kecik roots were macerated using methanol and chloroform. The result was then treated with E.coli and S.aureus with a concentration of 5 ppm and compared to negative control (solvent) and further observed and analyzed how reduced the bacterial growth with Two Ways ANOVA without interaction. The research results indicated that the sawo kecik roots methanol extract was effective to slow down the growth of S.aureus (0.160 ± 0.007) but ineffective against E.coli, whereas sawo kecik roots chloroform extract was not effective to reduce both bacteria. It can be concluded that further research is required to measure and verify the antibacterial activity of the extract using higher concentration samples and different research methodology.
Growth of Sweet Corn (Zea mays saccharata (Sturt.) Bailey) and Weed Density With Different of Fertilizer’S Doses Halim Halim; Tresjia Corina Rakian; Rachmawati Hasid; Resman Resman; Waode Siti Anima Hisein
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.9050

Abstract

 This study aimed to determine the response of the growth of sweet corn plants and weed density to the treatment of bio-fertilizer based on arbuscular mycorrhizal fungi and cow manure fertilizer. The study was conducted using a randomized complete block design (RCBD) in a factorial pattern. The first factor was arbuscular mycorrhiza fungi (AMF) consisting of four doses, namely without AMF (A0), AMF 5 g/planting hole (A1), AMF 10 g/planting hole (A2), AMF 15 g/ planting hole (A3). The second factor was cow manure fertilizer con-sisting of three doses, namely without cow manure fertilizer (B0), cow manure fertilizer 3 kg/plot (B1), cow manure fertilizer 6 kg/plot (B2). The treatment applied consisted of 12 combinations of treatments with 3 replications, so there were 36 experimental units. The observed variables were plant height, stem diameter, leaf area and weed density. The results showed that the average height of sweet corn was best at 42 DAP in the treatment of cow manure fertilizer 6 kg/plot (B2) as 111.6 cm. The average highest of stems diameter was at 14 DAP in the treatment of cow manure fertilizer 6 kg/plot (B2) as 0.40 cm. The average leaf area of sweet corn was at the age of 28 DAP was highest in the combination treatments cow manure fertilizer 6 kg/ plot (A0B2) and without AMF as 894.5. The weed species with the highest density found was I.cylindrica as 0.5-32.4%, C. kyllingia as 1.3-41.8%, C. rotundus as 1.3-30.3% and F.miliaceae as 1.2-12.7%. 

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