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Articles 482 Documents
(RETRACTED) Yield of Sorghum bicolor (L.) Moenh Genotypes in A Plastic Mulch Planting System in Gunungkidul Arif muazam; Setyorini Widyayanti; Budi Setiadi Daryono
Jurnal Biodjati Vol 8, No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.28840

Abstract

Sorghum is a strategic commodity with good nutritional and high antioxidant content that can be used as a source of food, feed, and industrial raw materials. Sorghum is a relatively low and less intensive crop than other staple food crops. Sorghum yield can be increased by establishing plant population through a mulching planting system. The purpose of this study was to determine the yield power of several sorghum genotypes, namely three new superior varieties (Bioguma, Kawali, and Samurai) and three local varieties (Plonco, Hitam Wareng, and Ketan Merah), planted with a plastic mulch planting system. The research was conducted on farmers' land in Karangmojo, Gunungkidul, Daerah Istimewa Yogyakarta, which is a dryland soil type. The experimental design used a randomized complete block design (RCBD) with 5 replications. Observation parameters included growth components and sorghum production. The results showed that sorghum planting with a mulching planting system was able to provide yields for the Bioguma variety of 7.70 tons/ha, Plonco variety of 8.41 tons/ha, Samurai variety of 5.38 tons/ha, Kawali variety of 8.21 tons/ha, Hitam Wareng variety of 3.91 tons/ha, and Ketan Merah variety of 4.00 tons/ha. Based on the results of the study, it was found that the genotypes of local varieties were able to compete with new superior varieties and had the best growth response and yield power in the rainy season planting.
Effect of Chitosan-Coated Centella asiatica Nanoparticles on Kidney Histology Profile of Complicated Diabetic Mice Bayyinatul Muchtaromah; Ana Mar'a Konita Firdaus; Eko Budi Minarno; Prilya Dewi Fitriasari; Mei Rhomawati; Maharani Retna Duhita; Wira Eka Putra
Jurnal Biodjati Vol 8, No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.21430

Abstract

Gotu kola (Centella asiatica) is a medicinal plant that has potential as an antioxidant and antidiabetic. Its compound is able to protect against diabetic nephropathy through the inhibition of oxidative stress. In terms of increasing drug potency and bioavailability of C. asiatica compounds, nanoparticle technology was used. This study aimed to determine the effect of C. asiatica nanoparticles coated with chitosan on the renal histology profile of complicated diabetic mice using a completely randomized design (CRD) with five treatment groups and five replications. The treatment groups were divided into K- (the experimental animals were not given any treatment), K+ (the experimental animals were induced by STZ), P1 (STZ + C. asiatica nanoparticles 120 mg/kgBW), P2 (STZ + C. asiatica nanoparticles 180 mg/kgBW), P3 (STZ + C. asiatica nanoparticles 240 mg/kgBW). The experimental animal model of complicated diabetes was induced by intraperitoneal STZ at a dose of 40 mg/kgBW for two days and STZ at a dose of 60 mg/kgBW for three days and then left for nine days. The C. asiatica nanoparticle therapy was given for 28 days. The parameters in this study were the results of scoring cell damage in the glomerular and tubular tissues (proximal and distal) of the kidney. The data obtained were tested for normality and homogeneity, then the normal and homogeneous data were tested with one-way ANOVA and further tested with Duncan's test. The statistical analysis results showed that C. asiatica coated with chitosan nanoparticles could reduce the damage to the histological profile of the glomerulus and tubules (proximal and distal) of the kidney of diabetic mice. C. asiatica-coated nanoparticles at a dose of 240 mg/kgBW showed the most optimal reduction in damage to the glomerular and tubular histology profiles.
Bandotan (Ageratum conyzoides L.) as Bio-Fungicide for Controlling Fusarium oxysporum in Chili (Capsicum annuum L.) Rochmah Agustrina; Nurul Fadhilla; Derlian Ella Tamara; Lili Chrisnawati; Yulianty Yulianty; Mahfut Mahfut
Jurnal Biodjati Vol 9, No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.27470

Abstract

Fusarium oxysporum L. is a fungus responsible for causing plant wilt disease in various horticultural crops. Meanwhile, Bandotan (Ageratum conyzoides L.) is commonly known as a weed that contains secondary metabolites with antifungal activity. Therefore, this study aimed to determine the effect of Bandotan stem and leaf extract on the growth of Fusarium oxysporum in chili (Capsicum annuum L.) infected during the germination phase. Evaluation of stem and leaf extract was carried out separately using a one-factor Complete Randomized Design (CRD). A total of four experimental units were used consisting of C+ (sprouts not infected with Fusarium sp. and not given stem or leaf of Bandotan extract), C- (sprouts infected with Fusarium sp. but not given stem or leaf of Bandotan extract), T1 (sprouts soaked with stem or leaf of Bandotan extract and infected with Fusarium sp.), and T2 (sprouts infected with Fusarium sp., and given stem or leaf of Bandotan extract). Each experimental unit was repeated five times, and parameters observed included disease severity, as well as dry and wet weight. The results showed that based on ANOVA test at the 5% significance level, the administration of 60% dry Bandotan stem extract to chili sprouts before Fusarium sp. infection significantly reduced disease severity. However, when the treatment was carried out after sprouts were infected, disease severity was not reduced. The administration of Bandotan leaf extract to sprouts both before and after being infected with Fusarium sp. reduced disease severity but a decrease in dry weight was also observed
Antibacterial Activity of Scurrula ferruginea (Roxb. Ex Jack) Leaves Extract Against Staphylococcus aureus and Escherichia coli Dwi Aditiyarini; Nita Sonia Sipayung; Vinsa Cantya Prakasita
Jurnal Biodjati Vol 9, No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30445

Abstract

The mistletoe (Scurrula ferruginea) is a parasitic plant obtaining nutrients and water from host plants at Nglinggo tea plantation, Kulon Progo, Yogyakarta. Despite the numerous benefits of Scurrula ferruginea leaf, substantiation regarding its pharmacological activity is still lacking. Therefore, this research aimed to identify Scurrula ferruginea species and obtain secondary metabolites with antibacterial activity. The experiment was carried out by extracting Scurrula ferruginea leaf using maceration methods with 96% ethanol, followed by qualitatively and quantitatively phytochemical screening, inhibitory zone test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. Several compounds, such as flavonoids, tannins, phenols, saponins, alkaloids, terpenoids, and steroids, were identified in the mistletoe extract. The results showed total flavonoid, phenolic, and tannin levels were 7.41 ± 0.38 mg QE/mg, 1672 ± 50.99 mg GAE/g, and 1.43 ± 0.15 mg TAE/g, respectively. Scurrula ferruginea leaf ethanolic extract showed the potential to form an inhibition zone against S. aureus in the moderate category at 525 mg/mL, but no significant activity was observed against E. coli. The MIC results of Scurrula ferruginea leaf ethanolic extract against S. aureus were 0.6%, and the MBC was at 2.4%. Furthermore, the MIC value against E. coli was 14%, and the MBC value was 28%. Based on these results, it could be concluded that Scurrula ferruginea leaf ethanolic extract showed high effectiveness in inhibiting and killing S. aureus bacteria compared to E. coli, with MIC and MBC values of 0.6% and 2.4%, respectively
Quercetin Compound of Okra Fruit (Abelmoschus esculentus L.) as Potential of Antioxidant and Antimicrobial Christina Astutiningsih; Jesi Kristanti; Winda Ludfi Ariani
Jurnal Biodjati Vol 8, No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.20888

Abstract

Respiratory tract infections can be caused by various microbes including Staphylococcus aureus bacteria and Candida albicans fungus. The treatment can use antibiotics or medical plants. Okra plants especially the fruit, are plants with antioxidant and antimicrobial activity caused by one of the flavonoids namely quercetin compounds. The purpose of this study was determine the total flavonoid, identify the isolate, and test the antioxidant and antimicrobial activity of the quercetin compound isolated from okra fruit. The result of the measurement of the total flavonoid content of the diethyl ether fraction obtained an average of 54.8723 ± 0.8059 mg QE/g and the ethyl acetate fraction obtained an average of 99.3155 ±1.4518 mgQE/g. The isolated flavonoid from okra fruit had antioxidant power with IC50 of 31,4 ppm, antibacterial activity against Staphylococcus aureus with inhibition zone diameter of 10,372mm; 13,964 mm; and 15, 392mm (1%:5%:10%), and the antifungal Candida albicans with inhibition zone diameter of 9,104mm; 284mm; and 11,380 mm (1%:5%:10%). Flavonoid isolates from Okra Fruit, namely quercetin compounds had strong antioxidant and antimicrobial activity.
Exploration and Identification of Ectomycorrhizal Fungi at IPB University Campus Forest Adilah Adawiah; Dinda Rista Mufidah; Ivan Permana Putra
Jurnal Biodjati Vol 8, No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.26109

Abstract

The exploration of ectomycorrhizal fungi diversity around the campus could provide insights into the presence and distribution of these symbiotic fungi in the area. This study aimed to observe, identify, and provide information regarding ectomycorrhizal fungi diversity in the IPB University Campus Forest. The sampling sites represent various tree species including forests, parks, and green spaces. The exploration was done using opportunistic sampling method. The obtained basidiomata were examined for macromorphological and micromorphological examination to identify the ectomycorrhizal fungi. The identification results based on the morphological characteristics confirmed our specimens as Inocybe cf. squarrosolutea (Corner & E. Horak) Garrido and Suillus bovinus (L.) Roussel. The Inocybe was characterized by having small to medium-sized, pileus squamules, dry surface, light brown to yellowish cap, lamellae crowded, cylindrical or attenuated stipe towards the apex, clavate basidia, and hemispheric knobs basidiospores. The Suillus was distinguished by having a cap that is convex in shape with a slightly brownish yellow color and the surface texture of the cap is smooth, lamella in the form of pores that are quite large and angular, brownish yellow in color, yellow tubular stipe, clavate basidia, and oval spores. These two macrofungi are new records in the sampling area and I. cf. squarrosolutea could be the new information for Indonesia. Our finding provides valuable information for ecological studies and contributes to the additional data on Indonesian ectomycorrhizal fungi diversity.
Antidiabetic Potential of Ethanol Extract from Moringa oleifera Leaves in Streptozotocin-Nicotinamide-Induced Female Mus musculus Vinsa Cantya Prakasita; Nadya Aprina Theodora; Enjelin Anjung Susilowati; Dwi Aditiyarini; Aniek Prasetyaningsih
Jurnal Biodjati Vol 9, No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30699

Abstract

Type 2 diabetes mellitus (DM) is a chronic metabolic dis­order characterized by elevated blood glucose levels due to insulin resistance. Type 2 diabetes is considerably more prevalent than oth­er forms (85–90%). The risk of type 2 diabetes is higher in women (53.2%). There is an urgent requirement for better and more afforda­ble treatment options considering DM therapy is expensive and may have adverse health effects. The study's objective is to examine how acute toxicity, blood glucose levels, and body weight are affected by Moringa leaf ethanol extract (MLEE). Maceration was employed to eliminate the leaves of the moringa plant. Phytochemical screening was completed to assess the total flavonoid content and screen for alkaloids, flavonoids, phenolics, saponins, and tannins. Acute toxicity testing was performed following OECD guideline 423. Clinical symp­toms of acute toxicity were observed every 30 minutes for the first 24 hours post-treatment, followed by observations every 24 hours up to 14 days. The estimated LD50 range was determined. Streptozotocin/ nicotinamide-induced female Mus musculus was administered to eval­uate the antidiabetic potential of MLEE. Six groups of mice were uti­lized, which included a healthy control group (aquades not induced), a negative control group (induced aquades), a positive control group (induced glimepiride 0.8 mg/kg BW), and three treatment groups with varying dosages of MLEE (induced; 0, 100, and 150 mg/kg BW). A semi-auto chemical analyzer was employed on days 0 through 31 to determine blood glucose levels. An analytical digital balance was utilized to calculate the body weight. With a total flavonoid concen­tration of 20.75%, MLEE incorporated alkaloids, flavonoids, pheno­lics, saponins, and tannins. MLEE demonstrated a significant effect in lowering blood glucose levels at a dose of 100 mg/kg BW (P<0.05). A significant positive correlation has been identified between body weight and blood glucose levels (P<0.05).
Phenolic Content and Antioxidant Activity Water and Ethanol Extracts of Sungkai Leaves (Peronema canescens Jack) Delima Engga Maretha; Dwi Fitri Yani; Lili Silvana; Mashuri Masri
Jurnal Biodjati Vol 8, No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.26777

Abstract

Sungkai (Peronema canescens Jack) is a plant that has phenolic content as an antioxidant that can enhance the immune system (immunomodulator). This study aimed to determine the phenolic content and antioxidant activity of Sungkai leaves (Peronema canescens Jack). The method  of antioxidant with invitro using of the DPPH where the ability of a compound to inhibit DPPH radicals is calculated from the absorbance value using UV-Vis spectroscopy. And Determination of total phenolic content was carried out using UV VIS spectroscopy at a wavelength of 745 nm with the help of Folin Ciocalteu reagentand a nd gallic acid standard curve . The test results showed that the water and ethanol extracts with a concentration of 0.1% had a phenolic content of 0.002% and 0.007%, their antioxidant activity (IC50) with a concentration of 0.02; 0.04; 0.06; 0.08; and 0.1% in the water and ethanol extracts of Sungkai leaves have values of 0.025% and 0.03% respectively. Therefore the best antioxidant activity (IC50) was extracts ethanol of sungkai leaves with phenolic content  of 0.007% and IC50  of 0.03%.
Metallothionein Protein Modeling from Pseudomonas aeruginosa PAO1 as A Metal Biosorber Candidate Fajri Ikhsan; Ahmad Shulhany; Syarif Abdullah
Jurnal Biodjati Vol 8, No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.29170

Abstract

Metallothionein is a protein that is well known to play a role in metal metabolism in bacterial cells. Metallothionein is a multifunctional protein that has the potential to be used as a metal adsorbing agent. Pseudomonas aeruginosa is a ubiquitous gram-negative and rapid-growth bacterium. In addition, the complete genome of Pseudomonas aeruginosa has been largely known. Pseudomonas aeruginosa PAO1 is a strain of Pseudomonas aeruginosa that the complete genome of this strain is easily accessible in NCBI. These features make Pseudomonas aeruginosa PAO1 become a common model in bacterial studies. This research aimed to find and model the putative metallothionein of Pseudomonas aeruginosa PAO1. This research was carried out by bioinformatic and protein homology methods. Based on the results, the putative metallothionein of Pseudomonas aeruginosa PAO1 was found in the bacterial genome at base sequence of 2355918 to 2356157. The putative metallothionein-encoding gene of Pseudomonas aeruginosa PAO1 has a size of 240 bp. The translation result of the gene showed that the putative metallothionein of Pseudomonas aeruginosa PAO1 has 79 amino acids. The modeling result showed the 3D structure of the putative metallothionein of Pseudomonas aeruginosa PAO1 is similar to the metallothionein 3D structure of Pseudomonas fluorescens Q2-87. The 3D structure of the putative metallothionein of Pseudomonas aeruginosa PAO1 was dominated by turn and coil, but contained 1 α-helix structure and 2 β-sheet structures. Based on protein analysis, it was found that the putative metallothionein of Pseudomonas aeruginosa PAO1 has 1 metal-binding cluster with 10 amino acids and the most important amino acid residue is Cysteine . Even though, there was 1 Histidine amino acid residue on the metal-binding cluster.
Community Structure of Avifauna in The Rehabilitation Zone at Wonoasri Resort, Meru Betiri National Park Arif Mohammad Siddiq; Hari Sulistiyowati; Munaa Aqidatul Ulaa; Fuad Bahrul Ulum; Firda Fadri
Jurnal Biodjati Vol 9, No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30033

Abstract

The rehabilitation zone of Meru Betiri National Park (MBNP) needs to be measured in relation to the success of ecosystem restoration. It can be used as a bioindicator, such as the bird community structure, hence their sensitivity to environmental changes. Therefore, the aims of this study are to determine the community structure of avifauna in the rehabilitation zone at Wonoasri Resort, MBNP including bird diversity, richness, dominance, feeding guilds, and their correlation with forest cover. The research was conducted in January 2023 in the Bonangan Block at three observation points, namely low vegetation cover, medium vegetation cover, and high vegetation cover. The ecological data, such as species and abundance, were collected using the point count method, while additional information related to conservation statuses were collected by web browsing on the IUCN Red List, the CITES appendix, and Indonesian government regulation (LHK No.106/2018). Data analysis used the dominance index, species diversity index (Shannon Wiener), and evaluated for statistical data using a Kruskal-Wallis (KW) test in R version 3.2.1. We recorded 38 bird species belonging to 31 genera and 21 families during this study. Among the feeding guilds, the insectivorous birds (14 species) were recorded as the higher species richness, followed by omnivorous (eight species), frugivorous (five species), granivorous (five species), carnivorous (four species), and nectarivorous (two species). The Kruskal-Wallis test shows there is a significant correlation among the feeding guilds (value of 11.644, with p-value = 0.040). Furthermore, referring to species richness, high vegetation cover areas have the highest species richness (30 species) compared to medium vegetation cover areas (27 species) and low vegetation cover areas (26 species). The dominance of avifauna species at this location falls into the low category (C = 0.07). According to the diversity index, avifauna in Bonangan Block is classified as a high category (H'=3.01).