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INDONESIA
Jurnal Biodjati
Published by Universitas Islam Negeri Sunan Gunung Djati Bandung
ISSN : 25481606     EISSN : 25414208     DOI : 10.15575/biodjati
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences
Arjuna Subject : -
Articles 482 Documents
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Physiological and Anatomical Responses of Red onion (Allium cepa L.) to Drought Stress after Biofertilizer Application Lestari, Nurrisma Ika; Siswanti, Dwi Umi
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.38613

Abstract

Red onion (Allium cepa) is a plant that requires sufficient water and is susceptible to drought stress. The use of biofertilizer is employed because it contains microorganisms that enhance nutrient availability and assist plant growth under abiotic stress conditions. This study aims to analyze the physiological and anatomical responses of red onion bulbs following the application of biofertilizer under drought stress. The doses of biofertilizer used were 0; 10;15; and 20 L/ha with field capacity levels of 25; 50; 75 and 100%. The parameters tested were bulb diameter, reducing sugar content number of bulb layers, anatomy of root cortex thickness, anatomy of root metaxylem diam Red onion eter, and fresh weight of red onion roots. The method used in determining the level of reducing sugar is the Nelson-Somogyi method. The method used for preparing the red onion root specimens was the embedding method. The results showed that the largest red onion bulb diameter was obtained from treatment A1B2, without the addition of biofertilizer at 75% field capacity. Meanwhile, the highest reduced sugar content was found at a dose of 15 L/ha with 50% field capacity
Phytochemical Constituent, Antibacterial and Antioxidant Leaves of Dracaena trifasciata (Prain) Mabb. Dewatisari, Whika Febria; To'bungan, Nelsiani
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33528

Abstract

Sansevieria, commonly known as Dracaena trifasciata. Apart from serving as an ornamental and air-purifying plant, it also exhibits medicinal properties, making it a potential candidate for the development of novel natural drugs. The demand for natural antibacterial agents has significantly increased due to the rising incidence of resistance among pathogenic bacteria, such as Staphylococcus aureus. This research conducted to determine the antibacterial and antioxidant activities of D. trifasciata leaf extracts. The D. trifasciata leaf material was sequentially extracted using sonication, beginning with chloroform and then followed by ethanol. The Kirby-Bauer method was employed to conduct the antibacterial test, wherein the inhibition zones generated by the chloroform and ethanolic extracts were measured. The test bacteria used in this study was Staphylococcus aureus. The assessment of the antioxidant activity was conducted using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Phytochemical compound identification in the extracts was conducted using GC-MS analysis. The ethanolic extract showed a higher yield percentage compared to the chloroform extract. Both extracts exhibited moderate antibacterial activity. The chloroform extract exhibited an inhibition zone of 73.33 mm2, while the ethanolic extract showed an inhibition zone of 110 mm2. The chloroform extract had a minimum inhibitory concentration (MIC) of 500 mg/mL and a minimum bactericidal concentration (MBC) of 1000 mg/mL. The ethanolic extract had an MIC of 31.25 mg/mL and an MBC of 62.50 mg/mL. In terms of antioxidant activity, the chloroform extract had an IC50 of 370.8±0.07 µg/mL, while the ethanolic extract had an IC50 of 647.4±0.12 µg/mL. GC-MS analysis revealed 47 compounds in the chloroform extract and 49 compounds in the ethanolic extract. Based on the results of yield, antibacterial and compound identification, the ethanolic extract of D. trifasciata leaves exhibited greater potential compared to the chloroform extract. Its antibacterial activity showed more promise than its antioxidant activity
Assessing Coral Reef Health in Northern Waters of Penata Besar Island West Kalimantan Bariah, Anisah; Safitri, Ikha; Nurrahman, Yusuf Arief; Sofiana, Mega Sari Juane; Helena, Shifa; Faizal, Ibnu
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.38146

Abstract

Penata Besar Island is designated as one of the Coastal and Small Island Conservation Areas (KKP3K) in West Kalimantan, is a critical site for conserving coral reefs and reef fish. Currently, global climate change and anthropogenic activities have exacerbated coral reef bleaching worldwide. In the context of escalating global climate change and human-induced threats, this study evaluates the health condition of coral reefs in the northern waters of Penata Besar Island, Bengkayang Regency. An exploratory approach was employed, utilizing the Line Intercept Transect (LIT) method across four stations at two distinct depths (2-3 m and 5-6 m). The results revealed that coral cover varied with depth. At a depth of 2-3 m, live coral coverage was between 51.2% and 62.4%, classified as good, while at 5-6 m, coverage ranged from 30.4% to 49.4%, categorized as fair. Coral Massive (CM) was the predominant growth form observed. The diversity index (H') ranged from low to medium, the Evennes index (E) from medium to high, and the dominance index (C) was categorized as low. These findings highlight the varying health of coral reefs in different depths and provide a basis and essential insights for targeted conservation efforts.
Antioxidant Activity and Pancreatic Lipase Inhibition of Curcuma aeruginosa Roxb Rhizome Fractions Suharsanti, Ririn; Wahyuono, Subagus; Yuniarti, Nunung; Astuti, Puji
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33900

Abstract

Antiobesity with a lipase inhibitor mechanism will block the hydrolysis of triglycerides into fatty acids and glycerol, while antioxidant compounds are very useful in conditions of obesity to prevent excess damage from degenerative diseases. Curcuma aeruginosa Roxb extract has been proven to have the effect of inhibiting pancreatic lipase so further effects will be seen at the fraction level. The purpose of this research is to investigate the phytochemical components ,antioxidant activity and pancreatic lipase inhibition of Curcuma aeruginosa Roxb fractions. Ethanolic extract of the Curcuma aeruginosa Roxb rhizome was separated using the solid-liquid chromatography with 3 different solvents (n-hexane, ethyl acetate, ethanol) to give n-hexane (HF), ethyl acetate (EAF), and ethanol (EF), and the insoluble (IF) fractions. Each fraction detected phenolics, flavonoids, alkaloids, tannins, saponins, triterpenoids/steroids. EF has the highest total flavonoid and phenolic content. Antioxidant activity of all fractions were measured using DPPH reduction, ABTS, and FRAP methods. The best antioxidant activity of all fractions using the DPPH method was EF with IC50 21.93 ± 3.39µg/mL, ABTS method was HF with IC50 24.56±1,03 µg/mL and FRAP method was IF with IC50 20.79±1,03 µg/mL. Totals of phenolics and flavonoids in EF strongly support the antioxidant activity of the DPPH method. The highest inhibition of pancreatic lipase was found in EAF at 35.16±0.24 % (100 µg/ml). There was significant difference between EAF and xenical (orlistat) (p
In Vitro Evaluation of Green Tea Extract's Influence on Local Sheep Rahmatillah, Ririn Siti; Ramdani, Diky; Hernaman, Iman; Jayanegara, Anuraga
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.34573

Abstract

In recent years, the exploration of natural supplements such as green tea extract and their potential benefits in livestock nutrition has gained considerable attention among researchers and agriculturalists. Green tea extract contains a high concentration of bioactive substances, such as polyphenols and catechins. This study aimed to explore the effects of varying doses of green tea extract on rumen fermentation parameters through an in vitro trial using sheep rumen liquids. A completely randomized experimental design was utilized to test the effect of 4 different doses of green tea extract treatments (0, 140, 280, and 560 mg/kg) on in vitro Hohenheim gas test method. The current study shows no significant effect of administering green tea extract at various doses on total gas production, total gas production per in vitro dry matter and organic matter degradability, volatile fatty acid per in vitro dry matter and organic matter degradability, methane production per inv vitro dry matter and organic matter degradability of green tea extract. Although no significant effects were observed on the investigated parameters, this study provides important insights in the understanding of the potential use of green tea extract in modulating fermentation processes in the ruminant’s stomach. In conclusion, the findings of this study underscore the impact of GTE on gas production regulation, highlighting its potential implications for gastrointestinal health
Antibacterial Activity of Dendrophthoe pentandra (L.) Leaves Extract Against Staphylococcus aureus and Escherichia coli Inggrid, Annabelle Marie; Aditiyarini, Dwi; Prakasita, Vinsa Cantya
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.30436

Abstract

Sapuran, Wonosobo is a popular area with several local tea plantations, which are infested by mistletoe, such as Dendrophthoe pentandra (L.) Miq. Mistletoe has been widely reported to have antibacterial activity due to its secondary metabolite content. Therefore, this study aimed to identify and explore the antibacterial activity of D. pentandra leaves extract against Staphylococcus aureus and Escherichia coli. The test samples were extracted using the maceration method, followed by phytochemical screening and total assay of flavonoids, phenols, and tannins. Antibacterial activity tests were carried out using disk diffusion, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC). The yield from the maceration method using 96% ethanol solvent was 8.78%, containing secondary metabolite compounds, including alkaloids, saponins, steroids, terpenoids, and flavonoids. The total flavonoid, phenol, and tanin content obtained were 4.09 ± 0.70 mg QE/g, 1.76 ± 0.16 g GAE/g, and 1.37 ± 0.14 mg TAE/g, respectively. In addition, the extract showed medium inhibition of S. aureus at a concentration of 400 and 525 mg/m, with no inhibitory effect against E. coli. The MIC and MBC from the microdilution method for S. aureus were at 4400 and 8800 mg/mL extract, while values of 28000 and 56000 mg/mL were obtained for E. coli. Based on these results, the inhibition activity of ethanol extract of D. pentandra against S. aureus was greater compared to E. coli.
Purification and Characterization of Cellulase of Mold Isolated from Vermicomposting Process of Palm Oil Empty Fruit Bunches Dewi, Rosita Fitrah; Muzakhar, Kahar
Jurnal Biodjati Vol 3 No 1 (2018): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i1.2292

Abstract

In previous investigation of vermicomposting process of palm oil empty fruit bunches, five cellulase-producing microorganisms had been isolated. An isolated VTM1 identified as Aspergillus sp. as a cellulase producing mold, and proven produced extracellular cellulase in solid state fermentation of palm oil empty fruit bunches. The obtaining crude cellulase was purified to homogeneity by ammonium sulfate precipitation, gel filtration on Sephadex G-100 chromatography, and finally purified on ion exchange chromatography on DEAE Cellulofine A-500. The yield and purification fold were 12.89% and 107.50, respectively. The purified cellulase was easily released glucose when 5% CMC applied as substrate, as shown by TLC analysis. The purified cellulase had an optimal pH and temperature at 4.0 and 45°C, and was stable at pH 3-7 and 30-50°C, respectively.
Kajian Perbandingan Luas Pekarangan dan Kearifan Lokal Jenis Tanaman Obat di Pesisir Pantai Kabupaten Tanah Laut Kadarsah, Anang; Susilawati, Ika Oksi
Jurnal Biodjati Vol 3 No 1 (2018): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i1.2346

Abstract

Minimnya informasi mengenai lahan pekarangan di pesisir pantai Kabupaten Tanah Laut serta terbatasnya pengetahuan penduduk lokal dalam mengenal berbagai jenis tanaman berkhasiat obat pada pekarangan berdampak terhadap diabaikannya peran tanaman obat di pekarangan dan tingginya biaya pemeliharaan penduduk lokal untuk kesehatan. Tujuan penelitian ini adalah mengkaji perbandingan luas pekarangan dan menggali informasi kearifan lokal berbagai jenis tanaman obat dari lima desa di Pesisir Pantai Kabupaten Tanah Laut, Provinsi Kalimantan Selatan. Metode yang digunakan adalah pengamatan jenis-jenis tanaman obat serta wawancara pengetahuan penduduk lokal dalam memanfaatkan pekarangannya. Hasil penelitian menunjukkan rerata luas pekarangan rumah tertinggi di Desa Takisung (212 m2) dan terendah di Desa Sungai Bakau (49,1 m2). Perbandingan luas ideal (2:3) antara pekarangan dengan rumah ditemukan di Desa Batakan dan Desa Sungai Rasau. Jumlah jenis tanaman obat pada pekarangan berkisar dari 30 -52 jenis dengan Indeks Nilai Penting (INP) terbanyak adalah mangga, pisang talas, singkong, karet dan kelapa. Indeks diversitas tanaman obat pada pekarangan menunjukkan nilai bervariasi, tertinggi di Desa Sungai Rasau (1,390) dan terendah di Desa Sungai Bakau (1,130).  Dalam hal ini, potensi terbaik pengembangan tanaman obat pada pekarangan berada di Desa Takisung berdasarkan parameter keanekaragaman jenis tanaman obat dan tipe pekarangan rumahnya.
Uji Aktivitas Bakteriofage Litik dari Limbah Rumah Tangga Terhadap Salmonella Typhi Jatmiko, Yoga Dwi; Purwanto, Agung Putra; Ardyati, Tri
Jurnal Biodjati Vol 3 No 2 (2018): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i2.3471

Abstract

Salmonella Typhi merupakan salah satu bakteri yang menjadi agen penyakit bawaan makanan. Bakteriofage sebagai alternatif penggunaan antibiotika telah digunakan untuk mengendalikan bakteri tersebut. Penelitian ini dilakukan untuk mendapatkan isolat bakteriofage litik yang mampu melisis beberapa bakteri patogen yang diujikan dan mengetahui pengaruh aktivitas bakteriofage litik terhadap pertumbuhan SalmonellaTyphi. Bakteriofage diisolasi dari limbah rumah tangga. Selanjutnya penentuan host range bakteriofage terhadap bakteri patogen lain dilakukan dengan metode spot test. Uji aktivitas bakteriofage terhadap SalmonellaTyphi dilakukan menggunakan metode bacterial challenge test. Berdasarkan hasil isolasi, didapat enam isolat bakteriofage, yaitu B2-St, B3-St, S1-St, S2-St, SL1-St, dan SL3-St. Semua isolat bakteriofage mampu melisiskan sel bakteri Escherichia coli dan Salmonella Typhimurium namun tidak mampu melisiskan Bacillus cereus, Staphylococcus aureus dan Shigella disentriae.Tiga isolat bakteriofagetelah terpilih berdasarkan densitas plaque terbanyak yaitu B2-St, SL3-St dan S2-St. Kemampuan isolat bakteriofage B2-St dalam melisiskan sel Salmonella Typhi lebih tinggi (6,81 ± 0,35 log sel/mL) daripada isolat bakteriofage SL3-St (7,39 ± 0,31 log sel/mL) dan S2-St (7,60 ± 0,27 log sel/mL). Penurunan densitas sel inang terendah oleh ketiga isolat bakteriofage terjadi pada jam ke-4. Bakteriofage B2-St merupakan bakteriofage terbaik dan berpotensi sebagai agen biokontrol Salmonella  Typhi. 
Growth and Flavonoid Content of Gynura procumbens (Lour) Merr. in Different Soil Water Content Savitri, Evika Sandi; Khusnia, Farrikhatun
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4446

Abstract

Gynura procumbens contains tannins, saponins, steroids, triterpenoids, essential oils and flavonoids which have bioactivity as a medicine for various diseases. One effort to obtain the quality of medicinal plants uses management of soil water content. The opti-mum of soil water content will support optimal growth of a plant. Whereas in conditions of low soil water content resulted in the induc-tion of the production of secondary metabolites as a self-defense sys-tem. This study aimed to determine the effect of soil moisture content on the growth and total content of plant flavonoids G. procumbens. This study was carried out experimentally using a one-factor com-plete randomized design (CRD) consisting of 4 treatments with five replications. The treatment used is the difference in soil water content of 40%, 60%, 80% and 100% field capacity. The data obtained in the form of quantitative data will be analyzed by One Way ANOVA Test. Measurement of the content of total flavonoid compounds was car-ried out using UV-Vis spectrophotometer analysis. The results of the study indicate the influence of soil moisture level on the growth and total content of G. procumbens. Parameter growth of fresh weight, dry weight, and root G. procumbens showed an increase in 40% soil water content. The flavonoid content showed the highest total flavo-noid content 18.884 mg/g in the 40% field capacity soil water content 

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