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INDONESIA
Jurnal Biodjati
Published by Universitas Islam Negeri Sunan Gunung Djati Bandung
ISSN : 25481606     EISSN : 25414208     DOI : 10.15575/biodjati
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences
Arjuna Subject : -
Articles 482 Documents
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Polyurethane Degrading Bacteria Isolated from Decayed Teak Wood (Tectona grandis Linn. f.) Aldila, Farah; Susilowati, Ari; Setyaningsih, Ratna
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4525

Abstract

Polyurethane is a class of polymers characterized by their substantial tensile strength and high melting points which enables them to be extremely durable. Despite its xenobiotic origin, polyure-thane has been found susceptible to biodegradation by microorgan-isms. The main object of this research was to investigate polyurethane degradation by the bacterium isolated from decayed teak wood (Tec-tona grandis Linn. f.). Polyurethane degrading bacteria were isolated using selective medium in the form of LB (Luria Bertani) with the ad-dition of 0.3% Impranil. Bacterial biodegradation activities shown by the changes of polyurethane structure analyzed by FT-IR spectrosco-py. The bacterial identification was carried out based on observations on the morphological characteristics of the colonies, cell morphology and sequences of 16S rRNA encoding genes. One of the potential iso-late that successfully isolated was K9, which demonstrates the disap-pearance of the 1735/cm peak of the characteristic function urethane in the FT-IR analysis. Analysis of the 16S rRNA encoding gene showed that the potential isolate having 98% similarity index to Bacillus safen-sis strain FO-36b. The activity shown by the isolate suggests that the bacteria could be a promising agent for polyurethane degradation. 
Genetic Relationship of the Transgenic and Non-Transgenic Mutiara Catfish with their Hybrids Sangkuriang Catfish Yolanisa, Iskarimah; Buwono, Ibnu Dwi; Mulyani, Yuniar; Iskandar, Iskandar
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4741

Abstract

The male broodstock of Transgenic Mutiara catfish (P) (containing Clarias gariepinus Growth Hormone, CgGH) is a fish who show fast growth. Propagation of the Transgenic Mutiara catfish F1 and F2 progeny involves the crossing of broodstock P with Non Transgenic Mutiara catfish. New genetic variations (polymorphisms) can be generated from crosses broodstock P and F1 which lead to the fast growth inheritance of transgenic fish. Phylogenetic relationship analysis showed distance and genetic variation in broodstock P, F1, F2 and Sangkuriang catfish (as controls) using the RAPD-PCR method (Random Amplified Polymorphic DNA-Polymerase Chain Reaction) as the research purposes. The results showed that OPA-03 (5'-AGTCAGCCAC-3 ') primer was selective for determining phylogenetic among catfish samples. The closest genetic similarity index originates from the female broodstock of Non Transgenic Mutiara catfish (P) (93%), while the farthest originates from the male broodstock of Mutiara Transgenic catfish (P) (51%) with F1 dan F2. Closely related broodstock catfish is not recommended to be crossed.
Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus Indriani, Reni; Prihastanti, Erma; Budihastuti, Rini; Nurchayati, Yulita
Jurnal Biodjati Vol 5 No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5840

Abstract

Tomatoes (Lycopersicon esculentum Mill.) are a source of carotenoids they are easy to find. These compounds function as precursors of vitamin A, antioxidant, and prevent cancer. The extraction of carotenoid compounds for commercial products usually uses fresh plants, which are less efficient and require a lot of raw materials. The supply of these raw materials can be done through tissue culture. The frequency of subculture or supply of nutrients in tissue culture is very influential on the content of callus carotenoids produced. This study aimed to determine the effect of subculture frequency on growth, development and callus carotenoid content and to find out the right frequency of subculture to produce callus with optimal growth, development and carotenoid content. The design this study was a single Completely Randomized Design (CRD) with 4 treatments of subculture frequency and 5 replications. The data obtained were analyzed by ANOVA at the 95% test level followed by DMRT in case a significant different was found The results showed subculture frequency affected growth, development and carotenoid content of callus Lycopersicon esculentum. Mill. The most optimal treatment to induce growth and production of carotenoids in this study was treatment of thrice subculture while the most optimal treatment in inducing development was the twice subculture treatment.
The Selection of Ornamental Plant for Landscape Design of Pollination Garden at Bogor Botanic Gardens Peniwidiyanti, Peniwidiyanti; Wanda, Irvan Fadli; Rinandio, Dipta Sumeru; Hutabarat, Prima Wahyu Kusuma; Hariri, Muhammad Rifqi; Setyanti, Dwi
Jurnal Biodjati Vol 5 No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.7480

Abstract

   Bogor Botanic Gardens is one of the outstanding green areas in Bogor City. Thousands of plant species are growing and providing suitable habitats and feed for various pollinators, such as butterflies, bees, and wasps. A well-designed pollination garden will benefit the pollinators and also create an educational facility for the community. This study aimed to select and characterize species of flowering plants that effectively attract pollinators, particularly Lepidoptera and Hymenoptera, in the pollination garden candidate area. The observa-tion had been carried out on 12 ornamental plant species: Pennisetum purpureum, Cosmos sp., Pachystachys lutea, Cuphea hyssopifolia, Orthosiphon aristatus, Lantana camara, Citrus aurantifolia, Buddleja davidii, Ixora javanica, Nerium oleander, Hibiscus rosa-sinensis, and Aglaia odorata. The method for observing pollinators was a standard walking method. Pollinator photos, its number, its visit time, and the plants it visited were recorded. A total of 23 species of Lepidoptera (20 species from 4 families and 3 species were unidentified) and 9 species of Hymenoptera (4 families) were found in the area with the best obser-vation time at 08.30-09.30 (GMT+7). The most visited plants by pollinators were Cuphea hyssopifolia, Lantana camara and Orthosiphon aristatus. Lepidoptera and Hymenoptera preferred blue-purple and bright-orange-yellow flowers. In addition, the flower characters that might influence pollinator visits are also described. The result would be an essential recommendation for plant selection, landscape design, and construction of the pollination garden in Bogor Botanic Gardens. 
Immunomodulatory Activity of Allium sativum, Curcuma mangga, and Acorus calamus Combination Nanoparticle on Mice Leukocytes Profile Muchtaromah, Bayyinatul; Analisa, Nur Izzah; Ahmad, Mujahidin; Fitriasari, Prilya Dewi; Habibi, Soraya; Hayati, Alfiah; Fajriyah, Ely Nuril
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.11879

Abstract

Immunomodulators are substances acting as stimulants or suppressants against the immune system, either to specific or non-specific activity. The non-specific through the production of leukocytes as the first response in fighting against an attacking antigen. Meanwhile, the specific, hrough recognition of antigens by lymphocytes during reattack. This research aimed to determine the immunomodulatory activity of the extracts combination nanoparticles of Allium sativum, Curcuma mangga, and Acorus calamus on the leukocyte profile of mice. This study used 5 treatments and 6 repetitions. The treatment groups were K- (untreated group), P1 (extracts combination nanoparticle dose of 25 mg/kg), P2 (extracts combination nanoparticle dose of 50 mg / kg), P3 (subur kandungan herbal medicine dose of 75 mg / kg), and P4 (Clomiphene citrate dose of 0.9 mg / kgBW). The parameters used included the total number of leukocytes and their differential value. The data that met the parametric assumptions, such as normally distributed and homogeneous were examined using the One Way ANOVA test, and when there was a significant difference, it was processed with the Duncan assessment. While those that did not meet the assumptions were evaluated using a non-parametric analysis. The statistical results showed that administration of extracts combination nanoparticles of A. sativum, C. mangga, and A. calamus at doses of 25 mg/kg and 50 mg/kg were able to suppress the inflammatory reaction by decreasing the total number of leukocytes. However, the differential leukocyte count was able to maintained or modulate immune system, indicating by the percentage of neutrophil, basophil, and eosinophil in the normal range. At a dose of 50 mg/kg, decreased the percentage of lymphocytes, while for monocytes, all dosage ranges were able to increase their number.
Potential of Lactic Acid Bacteria From Tape and Jember Tempeh as a Probiotic Candidate Azizah, Siti Nur; Eryani, Mikhania Christiningtyas; Azizah, Azizah
Jurnal Biodjati Vol 6 No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.12393

Abstract

Probiotics are microbes in fermented foods that have beneficial effects on health. Microbes that act as probiotics are lactic acid bacteria (LAB) that can produce metabolites such as lactic acid, hydrogen peroxide, and bacteriocins. This study aimed to obtain lactic acid bacterial isolates from tape and tempeh, and to test the potential of LAB as a probiotic candidate by activity test as an antidiarrhea and its resistance to gastric pH and bile salts. The fermentation products used as a source of LAB isolates are tempeh sumber mas merk, and yellow cassava tape, sari madu merk from Jember. The results of the first stage regarding the isolation of LAB using GYP media showed that there were 2 LAB isolates (TaJ.14 and TaJ.15) from the tape and 4 LAB isolates (TeJ.18, TeJ.22, TeJ.24, and TeJ.25) from tempeh. The results of the antidiarrheal test using the disc diffusion method (oxoid) showed that TaJ.14 and TaJ.15 isolates were able to inhibit Bacillus subtilis, Escherichia coli, and Shigella dysentriae, while TeJ.18, TeJ.22, TeJ.24, TeJ.25, and Lactobacillus casei (control) was only able to inhibit B. subtilis and E. coli. The results of LAB resistance to gastric pH showed that the TeJ.25 isolate had the highest percentage of pH 3 and 2.5 resistance (51.13 and 33.03%) compared to other isolates and controls. LAB resistance test results against bile salts (oxgal) showed that the TeJ.22 isolate had the highest percentage of resistance (75.10%) compared to other isolates although was still higher in control (75.99%).
Dinochloa Scandens (Poaceae-Bambusoideae): Distribution, Habitat Preference, and Notes on Synonymy Damayanto, I Putu Gede P.; Dalimunthe, Syadwina H.; Megawati, Megawati
Jurnal Biodjati Vol 6 No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.12485

Abstract

The distribution of Dinochloa scandens is unclear. World bamboo checklists suggest it is relatively widespread in Malesia. Here we clarify issues with herbarium specimen identification. Besides, a synonym name of D. scandens, namely D. macrocarpa collected from the Philippines, needs to be reviewed. The study aims to provide information on distribution and to review the synonym of D. scandens. The habitat preferences of D. scandens are also presented. We carried out targeted fieldwork at Gunung Halimun-Salak National Park in West Java in 2019 and extensive examination of herbarium specimens from Herbarium Bogoriense and online portals to accurately circumscribe this species. All data were analyzed descriptively. Distribution, altitude, and rainfall maps for D. scandens were produced with ArcGIS Pro. Our examination on the specimens suggests that D. scandens is indeed endemic to West Java and Banten Provinces in Indonesia. This bamboo species can be found in lowland to mountain forests with an altitude of 20-1400 m, in areas with annual rainfall between 3000-4000 mm. We support D. macrocarpa as an accepted name for a bamboo species from the Philippines and remove it from synonymy with D. scandens due to clear differences in the fruit compared to specimens of D. scandens across its range.
Characterization of Plant Growth Promoting Rhizobacteria (PGPR) on Capcissum annum Sari, Indah Juwita; Wahyuni, Indria; Khastini, Rida Oktorida; Awaliyati, Ewi; Susilowati, Andriana; Utari, Enggar; Aryantha, I Nyoman Pugeg
Jurnal Biodjati Vol 6 No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.13191

Abstract

Plant Growth Promoting Bacteria Rhizobacteria (PGPR) is one of the potential bacteria to enhance of Capsicum annuum through inhabitation the growth of pathogenic fungi. This study aimed to characterize PGPR in chili plants (Capsicum annuum). PGPR was isolated from the soil habitat of the red chili plant in Cilegon, Indonesia. Screening was then carried out with the dual culture method on Petri dishes and tested through in vivo method on the red chili plant. The selected bacteria were characterized morphologically, biochemically, and physiologically. The results revealed that there were 14 single isolates of bacteria from the roots of the red chili plants. The five single bacterial isolates, namely Azostobacter, Azospirillum, Pseudomonas, Serratia, and Beijerinckia have good potential as PGPR based on multiple culture screening by producing clear zones and positively effect the growth of chili plants.
Effect of Calina Papaya Leaves Extract on Respiratory Tract in Cigarette Smoke Exposed Rats Setiawan, Haris; Hikmawati, Arlinda Sekar Ayu; Wulandari, Sri Wijayanti; Malinda, Eriza Via; Putra, Ichsan Luqmana Indra
Jurnal Biodjati Vol 7 No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.19068

Abstract

Cigarette smoke is a source of free radicals that cause health problems throughout the world. Indonesia is a country that has many sources of natural antioxidants in counteracting free radicals, one of which is Calina papaya leaves. The study aimed to determine the potential of Calina papaya leaves antioxidants in improving the respiratory organs of Wistar rats after being exposed to cigarette smoke. The study using 25 male Wistar rats consisted of Control (not exposed to cigarette smoke and given distilled water), KN (cigarette smoke and given distilled water), P1 (cigarette smoke and Calina Papaya Leaves Ethanolic extracts (CPLE) 100 mg/kg BW), P2 (cigarette smoke and CPLE 200 mg/kg BW), and P3 (cigarette smoke and given CPLE 300 mg/kg BW). Exposure to cigarette smoke and administration of extracts was carried out for 21 days. On day 22, the rats were euthanized and dissected to remove the lungs and trachea. Organs were prepared using the paraffin method and hematoxylin-eosin staining. Parameters consisted of histopathological observations of the lungs and trachea. All data parameters were analyzed using a one-way ANOVA test and Duncan's advanced test (p<0.05). The results showed that there was an improvement in histopathology of the lungs and trachea at a dose of 200 mg/Kg BW compared to other doses (p<0.05). The study showed that the Calina papaya leaves ethanolic extracts have the potential as a source of antioxidants in improving the respiratory organs of Wistar rats after being exposed to cigarette smoke.
Genetic Characteristics of Chloropsis cochinchinensis Gmelin, 1789 Based on The Mitochondrial DNA COI Gene Jarulis, Jarulis; Saputra, Apin; Wibowo, Risky Hadi
Jurnal Biodjati Vol 8 No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.20260

Abstract

The rate of illegal poaching of blue-winged leafbirds (Chloropsis cochinchinensis) throughout Indonesia, particularly Bengkulu, is quite high. However, only minimal molecular information is available for this species. We performed mtDNA COI gene sequencing to explore genetic characters (conservative site, variable site, parsimony site, and singleton site) of blue-winged leafbirds. Using Qiagen's DNeasy® Blood and Tissue Kit based on the Spin-Column Protocol, total DNA was isolated, and PCR amplification methods were performed. DNA derived from a PCR reaction was forwarded to PT. First Base Malaysia for sequencing. Using MEGA 10.0 and BIOEDIT, the COI gene nucleotide sequence data were assembled, edited, and analyzed to explore of single nucleotide polymorphism, genetic distance, and phylogeny. The 616 bp COI genes contained 566 conservative sites (C), 50 variation sites (V), 24 information parsimony sites (Pi), and 26 singleton sites (S), as indicated by the results. The greatest nucleotide base composition was cytosine (34.1–34.9%), while the lowest was guanine (15.7–16.2%). The proportion of adenine-thymine nucleotide base pairs was greater than that of guanine-cytosine (50.3%). There were 26 barcode-specific mutation sites, 17 transition substitution mutation sites, and 9 transverse substitution mutation sites. The average genetic distance between C. cochinchinensis individuals was 2.2%, but the average genetic difference between species was 9.0%. All C. cochinchinensis individuals in our sample clustered within the same clade and were distinguished from other species within the same genus. The COI gene sequences of C. cochinchinensis that we acquired are novel and can be utilized for molecular identification of the species.

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