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Articles 482 Documents
Endemic Bamboo (Poaceae, Bambusoideae) of the Lesser Sunda Islands Damayanto, I Putu Gede P.; Rustiami, Himmah; Miftahudin, Miftahudin; Chikmawati, Tatik
Jurnal Biodjati Vol 8 No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.25015

Abstract

More than 40 species of bamboo were known from the Lesser Sunda Islands (LSI), including several endemic species. Endemic species are often highly specialized, found in limited geographical areas, and vulnerable to extinction if their habitat is destroyed. This study aimed to provide an account of endemic bamboo species on the LSI. Fieldwork involved collecting samples of bamboo in the LSI. Herbarium specimens of bamboo deposited in the Herbarium Bogoriense (BO), collected from the LSI were examined, and endemic LSI species were recorded. The conservation status followed the International Union for Conservation of Nature (IUCN) red list categories and criteria. The conservation status of unassessed species was evaluated using IUCN red list categories and criteria. A spatial analysis was also conducted. Data were analyzed and presented descriptively. There are 10 endemic bamboo species in the LSI, namely Bambusa ooh Widjaja & Astuti from Bali (conservation status: endangered), Chloothamnus reholttumianus (Soenarko) Widjaja from Sumba (vulnerable), C. schmutzii (S.Dransf.) Widjaja from Sumbawa and Flores (near threatened), Dinochloa kostermansiana S.Dransf. from Sumba and Flores (near threatened), D. sepang Widjaja & Astuti from Bali (vulnerable), Fimbribambusa rifaiana Widjaja from Alor (near threatened), Gigantochloa aya Widjaja & Astuti from Bali (vulnerable), G. baliana Widjaja & Astuti from Bali (vulnerable), G. taluh Widjaja & Astuti from Bali (vulnerable), and Schizostachyum purpureum Damayanto & Widjaja from Sumba (data deficient). They can be found at an altitude of 20–1700 m in various habitats, including forests, bamboo forests, villages, botanical gardens, along a road, slopes, valleys, riverbanks, and around a lake.
Genetic Variation of Structural and Functional Genes of SARS-CoV-2 Isolates Circulating in Banyumas (Indonesia) Rovik, Anwar; Noviani, Clearensia Ade Bina
Jurnal Biodjati Vol 8 No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.25132

Abstract

Scientists are performing various measurements to overcome the COVID-19 pandemic. The genomic mutations of SARS-CoV-2 can change their pathogenicity, infectivity, transmission, and antigenicity. This present study aimed to know a) the genetic variation of structural and functional genes of SARS-CoV-2 circulating in Banyumas and b) the potential of the Cilacap’s Harbour as a human mobility portal on the genetic variations of SARS-CoV-2 circulating in Banyumas, Central Java (Indonesia). Genomic sequence of SARS-CoV-2 isolates were taken from the Global Initiative on Sharing All Influenza Data (GISAID) and the National Center for Biotechnology Information (NCBI) online platforms. A gene cut was carried out from Wuhan reference isolate, fifteen isolates from Banyumas, and two isolates from Cilacap (Central Java) using Unipro UGENE v. 33.0 software, considering the annotation of the Wuhan-1 isolate. Genetic variations were detected among SARS-CoV-2 isolates circulating in Banyumas. The structural protein (envelope, membrane, nucleocapsid) encoding gene and the RdRp gene were highly conserved to Wuhan reference genome (Wuhan-Hu-1). Meanwhile, the ORF and the spike-encoding genes were less identical to the Wuhan reference genome. This study also proposed that human mobility from outside Central Java through Cilacap’s Harbour did not affect the genetic variation of SARS-CoV-2 isolates circulating in Banyumas (Central Java).
The Existence of Undergrowth at Forest Garden Stands in Grand Forest Park, Lampung Province Indriyanto, Indriyanto; Indriyanto, Nurani Hardikananda
Jurnal Biodjati Vol 8 No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.26670

Abstract

The existence of undergrowth has an important role as an ecosystem both from an ecological and economic perspective. However, the composition and diversity of undergrowth in an area depends on the land use system. This research aimed to know the species composition and diversity of undergrowth at forest garden stands. Data collection was carried out with a vegetation survey using nested rectangular plots with a sampling intensity of 1%. The data collected included undergrowth species, the number of individuals for each species, and tree species as components of forest garden stands. Data were analyzed by calculating density, frequency, important value index, and Shannon diversity index. The results showed that the undergrowth that exists under forest garden stands were 38 species dominated by 12 species, namely Acalypha australis, Asystasia gangetica, Clidemia hirta, Starchytarpeta jamaicensis, Mikania micrantha, Peperomia pellucidia, Setaria barbata, Andropogon aciculatus, Alpinia galanga, Diplazium esculentum, Paederia foetida, and Crassocephalum crepidioides. The forest garden stands were composed of 43 tree species dominated by 4 tree species, namely Durio zibethinus, Aleurites moluccana, Myristica fragrans, and Gnetum gnemon. The level of undergrowth diversity was in the medium category with an H value of 1.540, indicating the diverse of undergrowth species.
Mangifera spp. in the Ethnoclimatology Study of Tatangar Banjar Tradition in Kalimantan Hidayati, Eisya Hanina; Yuniati, Ratna; Lestari, Retno; Jumari, Jumari; Walujo, Eko Baroto
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.30048

Abstract

The biodiversity of Mangifera spp. not only supports the local economy and preserve food security but also safeguards cultural heritage through their role in ethnoclimatology studies within the Tatangar tradition of the Banjar community in Kalimantan. The Banjar community demonstrates a unique connection with nature by utilizing specific Mangifera species as crucial indicators of seasonal transitions, deeply ingrained in their cultural identity. The aim of this study is to reveal the prevalence of various Mangifera species, including endangered ones, in diverse landscapes, promoting a sustainable agroforestry system and conservation efforts. Qualitative and quantitative research methods were employed to explore and analyze Tatangar knowledge within the Banjar community in Mandiangin Barat Village, particularly its connections to climate and weather. The Banjar community further traditionally classifies Mangifera species, or known as “asam-asaman”, based on morphological traits, aiding in their interpretation of Tatangar signs. These signs, rooted in the flowering time of Mangifera trees, serve as essential climate indicators, guiding agricultural practices and influencing decisions on planting and harvesting. This local knowledge enhances agricultural resilience, enriches their cultural heritage, and underscores the intrinsic relationship between culture, nature, and sustainability in Kalimantan, making it a valuable subject for ethnoclimatological exploration
In Vitro Antioxidant and Anti-Obesity Activities of Ethanolic Extract from Microalgae Strain MRB-2 Ni’maturrohmah, Dwi; Darsih, Cici; Susanti, Hani; Hidhayati, Noor; Indrianingsih, Anastasia Wheni; Handayani, Sri; Hasby, Rizal Maulana; Laksitorini, Marlyn Dian
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30385

Abstract

Obesity has a 15-fold higher risk of coronary heart disease, stroke, and diabetes mellitus. Microalga isone of the natural resources that potentially treat obesity. The purpose of this study was to evaluate the total phenolic contents (TPC), antioxidant, and anti-obesity properties of ethanolic extract of microalgae strain MRB-2. The TPC was determined using the Follin-Ciocalteu method. The antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and the anti-obesity was analyzed using an anti-lipase pancreatic assay. The morphology of microalga cells was also determined using Scanning Electron Microscopy (SEM). The results revealed that the TPC of ethanolic extract from the ultrasound extraction method was higher than the maceration method with the value of 2.75±0.26 mg GAE/g. While the scavenging activity toward DPPH radicals of ethanolic extract from the maceration method was higher than ultrasound, with a value of 38.92±1.94% at 0.8 mg/mL. The lipase inhibitory activity of extract from the maceration method was higher than ultrasound with a value of 20.81±2.24% at 0.38 mg/mL. Our results indicate that ethanolic extract of MRB-2 was potentially developed for anti-obesity foods and health-functional foods derived from new peatland microalgae.
Effectiveness of Fruit Peels Eco Enzyme in Reducing Total Chromium from Yogyakarta Tanning Industry Waste Putri, Caecilia Radella Rivika; Jati, Alphonsus Wibowo Nugroho; Yulianti, Lorensia Indah Murwani
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.31077

Abstract

. Leather tanning industry is essential in Indonesia's economic sector, specifically in the export of hides and leather goods. The high production of goods is often accompanied by a large amount of waste disposal, containing elevated levels of chromium which has a negative effect on the environment. To address this problem, eco enzyme containing high carboxyl groups is expected to be an effective solution. Therefore, this study aimed to determine the functional groups in fruit peels eco enzyme and its effectiveness in reducing total chromium content in leather tanning waste. The experimental process included the preparation of eco enzyme formulations, testing functional groups with FTIR, and determining total chromium content using AAS. Furthermore, the characteristics of waste were determined, including such as BOD, pH, temperature, ammonia, TDS, and TSS, followed by incubation of eco enzyme in waste, as well as data analysis with ANOVA and Duncan test. The results showed that eco enzyme formulations contained hydroxyl (O-H) and carboxyl (-COOH) functional groups, carbon-carbon single bonds (C-C), carbon-oxygen single bonds (C-O), carbon-carbon double bonds (C=C), and carbon-hydrogen bonds (C-H). All formulations effectively reduced chromium levels in leather tanning waste to value lower than quality standards. Based on the results, the best formulation was found to be eco enzyme A, which comprised one type of fruit, namely mango peels.
Stevia Local Tawangmangu Generation M1 Result of Oryzalin Treatment Susanti, Dian; Parjanto, Parjanto; Haryanti, Sari
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.31634

Abstract

 Stevia rebaudiana Bert. (Stevia) is used in the commercial and health industries because of its steviol glycosides (stevioside, re­baudioside A, rebaudioside C) and flavonoids. Polyploidy induction of stevia plants using oryzalin was held to increase the diversity and produce superior varieties. This research aims to obtain stevia diver­sity with different characteristics from its diploid. The Tawangman­gu variety of stevia seedlings was experimentally designed using a completely random design. Nine combinations of oryzalin concentra­tions (1.5, 2.5, and 3.5μM) and immersion times (4, 6, and 8 hours) treatments were applied to 15 stevia seedlings each. Stevia without treatment was used as a control. Treatments were applied directly to the shoots on the second internode from the tip. Observations on tar­get shoots included the number of survivors, morphological variables (height, number of internodes, internode length, leaf size, leaf thick­ness, stem diameter, and leaf color), stomata, and plant ploidy level. Data analysis on the number of live plants used frequency and de­scriptively. Morphological data consisting of plant height, number of shoots, number of internodes, internode length, leaf length, leaf width, leaf thickness, and stem diameter were analyzed using boxplot graphs and descriptive to describe the diversity of M1 stevia treatment re­sults. Leaf color and stomata were analyzed descriptively. The results showed that up to 3.5μM concentrations of oryzalin and 6 hours of immersion time were safe to use as a mutation agent with above 67% survival rate. Various oryzalin treatments of Tawangmangu stevia va­rieties yielded polyploidy morphological growth indications in height, number of internodes, internode length, stem diameter, leaf size, leaf thickness, leaf color, stomata, and stem diameter. In addition, there are growth variations such as chimeras, rosettes, and leaf splitting. However, further flow cytometry tests showed that oryzalin concen­tration and immersion duration directly on the vegetative material did not produce polyploid stevia individuals.
In Silico Studies of Bioactive Compounds from Pseudomonas azotoformans UICC B-91 in Inhibiting Candida albicans Pratiwi, Rina Hidayati; Sulistyaniningsih, Endang; Jepri, Jumadi
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.32147

Abstract

Futoamide, Gentialutine, Gentiabetine, 1-[(2E,4E)-2,4-dec- adienoyl] pyrrolidine, Lycopodine, Dihydro-lycopodine are bioactive compounds that can be obtained Pseudomonas azotoformans UICC B-91. Previous research results indicate that P. azotoformans extract- ed with dichloromethane, chloroform, and ethyl acetate have antican- didal activity against Candida albicans ATCC 10231. C. albicans is considered to become an opportunistic pathogen and well-known as the main cause of candidiasis. This study aims to determine the mech- anism of inhibition of bioactive compounds from P. azotoformans UICC B-91 on the growth of C. albicans using the molecular docking method. Docking was carried out using the targeted (Lanosterol 14 alpha demethylase and Glucan endo-1,3-beta-D-glucosidase) dock- ing method with an exhausted parameter of 50. The size of the grid- box was adjusted to the position of the amino acid residues based on predictions of binding sites using PrankWeb. The docking results were obtained in the form of binding affinity resulting from the inter- action of the compound with the protein. Results showed that the three sample compounds had the potential to form strong and stable bonds with both protein targets with only two ligands show a binding energy value of less than -7 kcal/mol. In addition, the speed and stability of the bond between the sample and the target protein cannot exceed control ligands, thus it can be predicted the structure of ligand. Fu- toamide, 1-[(2E,4E)-2,4-decadienoyl] pyrrolidine, Lycopodine, and Dihydrolycopodine had binding potential with Lanosterol 14 alpha demethylase. For Glucan endo-1,3-beta-D-glucosidase, only Fu- toamide had the potential to form stable and strong bonds that similar to the control. It can be concluded that the futoamide, one of ligand from P. azotoformans compounds has the potential as a multitarget inhibitor of the two C. albicans proteins, because it has various affini- ties and interaction stability for Lanosterol 14 alpha demethylase and Glucan endo-1,3-beta-D-glucosidase.
Phytochemical Constituent, Antibacterial and Antioxidant Leaves of Dracaena trifasciata (Prain) Mabb. Dewatisari, Whika Febria; To'bungan, Nelsiani
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33528

Abstract

Sansevieria, commonly known as Dracaena trifasciata. Apart from serving as an ornamental and air-purifying plant, it also exhibits medicinal properties, making it a potential candidate for the development of novel natural drugs. The demand for natural antibacterial agents has significantly increased due to the rising incidence of resistance among pathogenic bacteria, such as Staphylococcus aureus. This research conducted to determine the antibacterial and antioxidant activities of D. trifasciata leaf extracts. The D. trifasciata leaf material was sequentially extracted using sonication, beginning with chloroform and then followed by ethanol. The Kirby-Bauer method was employed to conduct the antibacterial test, wherein the inhibition zones generated by the chloroform and ethanolic extracts were measured. The test bacteria used in this study was Staphylococcus aureus. The assessment of the antioxidant activity was conducted using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Phytochemical compound identification in the extracts was conducted using GC-MS analysis. The ethanolic extract showed a higher yield percentage compared to the chloroform extract. Both extracts exhibited moderate antibacterial activity. The chloroform extract exhibited an inhibition zone of 73.33 mm2, while the ethanolic extract showed an inhibition zone of 110 mm2. The chloroform extract had a minimum inhibitory concentration (MIC) of 500 mg/mL and a minimum bactericidal concentration (MBC) of 1000 mg/mL. The ethanolic extract had an MIC of 31.25 mg/mL and an MBC of 62.50 mg/mL. In terms of antioxidant activity, the chloroform extract had an IC50 of 370.8±0.07 µg/mL, while the ethanolic extract had an IC50 of 647.4±0.12 µg/mL. GC-MS analysis revealed 47 compounds in the chloroform extract and 49 compounds in the ethanolic extract. Based on the results of yield, antibacterial and compound identification, the ethanolic extract of D. trifasciata leaves exhibited greater potential compared to the chloroform extract. Its antibacterial activity showed more promise than its antioxidant activity
Effects of Wedelia Ethanol Extract on Sperm Quality and Blood Cholesterol Levels in Obese Rats Iffatuzzahra', Yumna Nabilah; Wardani, Diah Kartika; Hidayat, Andika Gundawa; Makhabati, Ulinuha Farah; Setiawan, Haris
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.37673

Abstract

Obesity is a complex, multifactorial condition contributing to male infertility by increasing oxidative stress, which negatively affects reproductive hormones, sperm quality, and blood cholesterol levels. The Wedelia plant (Sphagneticola trilobata (L.) Pruski) is a natural herbaceous plant containing flavonoids and tannins, known for enhancing sperm quality and inhibiting cholesterol synthesis in the blood. This study aims to determine the potential of Wedelia in improving sperm quality and regulating blood cholesterol levels. The study used 24 male Wistar rats divided into four groups, consisting of C (no HFF, distilled water only), NC (HFF, distilled water), T1 (HFF and 100 mg/Kg BW Wedelia extract), and T2 (HFF and 200 mg/Kg BW Wedelia extract). HFF was administered from weeks 1 to 3, followed with extract treatments from weeks 3 to 5. On day 36, the rats were euthanized for blood collection using hematocrit into EDTA tubes. The rats were dissected for the epididymis, which was then incised in a 10% PBS to release sperm. Sperm motility and count were observed on glass slides, while sperm viability and morphology were analyzed with hematoxylin-eosin using an Optilab microscope (400x magnification). The observation parameters included sperm morphology, count, motility, viability, total cholesterol, LDL, and HDL levels. Data were analyzed using ANOVA with DMRT post hoc testing (p ≤ 0.05). The results showed that T2 exhibited an increase in sperm count, motility, viability, and morphology in HFF-induced obese rats. T1 and T2 also showed enhanced HDL cholesterol and reduced total and LDL cholesterol levels. In conclucions, a Wedelia leaf extract dose of 200 mg/kg BW optimally improves sperm quality and reduces total and LDL cholesterol levels.