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INDONESIA
Jurnal Biologi Tropis
Published by Universitas Mataram
ISSN : 14119587     EISSN : 25497863     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Jurnal Biologi Tropis (ISSN Cetak 1411-9587 dan ISSN Online 2549-7863) diterbitkan mulai tahun 2000 dengan frekuensi 2 kali setahun oleh Program Studi Pendidikan Biologi PMIPA FKIP Universitas Mataram, berisi hasil penelitian dan ulasan Ilmiah dalam bidang Biologi Sains.
Arjuna Subject : -
Articles 2,520 Documents
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The Relationship Between The Total Count of Leukocyte Types and Formahldehyde Induction in Wistar Rats Anak Agung Bagus Tito Indra Prawira Negara; Anak Agung Ayu Niti Wedayani; Bayu Tirta Dirja
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6089

Abstract

Formaldehyde is a chemical compound with a high level of toxicity. In the industrial sector, formaldehyde is commonly found in cosmetic products, resins, adhesives, plastics, and wood products. Therefore, the objective of this research is to determine the effect of formaldehyde induction with concentrations of 20 ppm, 30 ppm, and 40 ppm on the total count of leukocyte types. This study is a qualitative research with a true experimental design employing a post-test only control group design. The study used male Wistar rats (Rattus norvegicus) totaling 24 individuals, which were divided into 4 groups: a control group and 3 treatment groups. Each group consisted of 6 rats induced with formaldehyde at concentrations of 20 ppm, 30 ppm, and 40 ppm. The Wistar rats will be terminated, and retro-orbital blood sampling will be conducted to count the various types of leukocytes in order to determine whether there are differences in leukocyte type counts among rats induced with formaldehyde. Data analysis will employ the Kruskal-Wallis test. The results of this study showed that there was a statistically significant difference (p <0.05) between formaldehyde and the total count of leukocyte types. In this study, it was found that there is a decrease in the total count of leukocyte types in the formaldehyde-exposed groups at 40 ppm, 30 ppm, and 20 ppm when compared to the control group.
The Effect of Feeding Non-Antioxidant Feed on The Degree of Dysplasia in Formaldehyde-Induced Wistar Rats Ni Putu Ayu Nindya Dewi Cahya Putri; Anak Agung Ayu Niti Wedayani; Ima Arum Lestarini; Abdul Qadar Punagi; Nova Audrey Luetta Pieter; Muhammad Husni Cangara; Novia Andansari Putri Restuningdyah
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6091

Abstract

Nasopharyngeal dysplasia is a condition of abnormal cellular growth that occurs due to the failure of nasopharyngeal epithelial cells in carrying out cellular adaptation. This condition can occur due to the continuous inhalation of toxic compounds, such as formaldehyde, which has genotoxic and cytotoxic effects that can induce cellular damage. One of the compound activity associated with reducing risk of dysplasia is antioxidant activity. Therefore, it is important to know whether the presence of antioxidant activity affects the occurrence of dysplasia. This study is an experimental study with a post-test only control group design which aims to determine the effect of Non-Antioxidant Feed on the occurrence of nasopharyngeal dysplasia. Testing of the antioxidant activity in feed was carried out using the DPPH assay. There were two groups of experimental animals including the treatment group (fed with Non-Antioxidant Feed) and the control group (fed with SB 11). All animals in each group were induced with 40 ppm formaldehyde by inhalation for 8 weeks and then euthanized using chloroform inhalation for nasopharyngeal tissue extraction. The results of the histopathological examination of the two groups were then statistically tested using the Mann-Whitney U test and obtained a value of P<0.05 (P=0.006), which means that there are effect of feeding Non-Antioxidants Feed on the degree of dysplasia in formaldehyde-induced wistar rats. The results can be associated with the ingredients contained in the feed given, differences in genetic polymorphisms of each subjects, and effect of body weight variations of the rats caused by randomization.
Antagonist Test of Bacillus subtilis ATTC 6633 and Trichoderma harzianum on the Growth of Magnaphorte oryzae on Several Varieties of Priming Rice Seeds Feskaharny Alamsjah; Zozy Aneloi Noli; Hesti Dwi Marcellinna; Anthoni Agustien; Suwirmen; Kurniadi Ilham
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6112

Abstract

Priming is a seed-soaking technique to increase seed viability and also suppress pathogens' growth. Magnaporthe oryzae is one of the pathogens in rice plants that causes leaf blast disease. The high rice consumption each year in Indonesia is not proportional to the amount of rice plant production, which is affected by the growth of pathogenic fungi. This study aims to determine the viability and percentage inhibition of Bacillus subtilis and Trichoderma harzianum against the growth of the pathogenic fungus Magnaporthe oryzae on local West Sumatra rice varieties Ceredek, Pandan Pulau, and Batang Sungkai. The research was conducted by testing the viability of microbes by counting the number of colonies and testing microbial antagonists with the Dual Culture method on seven days of observation. The results showed that Bacillus subtilis and Trichoderma harzianum could inhibit the growth of the pathogen M. oryzae, which causes leaf blast disease. The viability of Bacillus subtilis after biopriming for 48 hours was most significant on Ceredek variety, which was 15.9x106 cfu/g, and for Trichoderma harzianum on Pandan Pulau variety, which was 0.4x106 cfu/g. The most significant inhibition percentage of Bacillus subtilis and Trichoderma harzianum was obtained in Ceredek rice with a value of 30.13% (medium category) and 63.04% (high category).
Growth of Black Orchids (Coelogyne Pandurata Lindl) with Additional Ecoenzyme Liquid Organic Fertilizer and Biostimulant of Cengkodok Leaf Extract Zulfa Zakiah; Masnur Turnip
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6113

Abstract

Acclimatization of black orchid plantlets (Coleogyne pandurata Lindl.) with cengkodok leaf extract biostimulant treatment has been successfully carried out, but still have slow growth. To support growth and development at the enlargement stage, cultivated orchids need to be given eco enzyme liquid organic fertilizer (LOF) derived from various sources of organic matter. Provision of ecoenzyme LOF combined with biostimulant 20 mg/l of Cengkodok leaf extract to increase the efficiency of nutrient absorption. The aim of the research was to find the best type and concentration of LOF for the growth of black orchids at the enlargement stage. The study used a Complete Randomized Design (CRD) with two treatment factors namely type of LOF (ecoenzyme (ee) LOF from vegetable and fruit waste, ee LOF from pineapple peel waste, and "NASA" LOF) and . the POC concentration (0; 0.5; 1.0; and 1.5 ml/l). The results showed that the single treatment of ee LOF significantly effected the number of leaves and the number of black orchid tillers, while the single treatment of ee LOF concentration had an effect on the parameters of height, number of leaves, number of tillers and leaf area of black orchid. The parameter of chlorophyll content had no signifcant effect by the type and concentration of LOF. The largest leaf area was shown in the addition of ee LOF from vegetable and fruit waste at a concentration of 1 ml/l which was 11.88 cm2.
Protein Profile Analysis of Tungro and Dwarf Virus-Infected Rice Plants using SDS-PAGE Saipul Abbas
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6120

Abstract

Viruses in rice plants are one of the causes of decreased harvest yields and rice farming productivity. This study aims to analyze the protein profile of viruses in rice plants infected with tungro and dwarf viruses using the SDS-PAGE method. The study includes six plant samples, including tungro-infected plants from Pinrang and Sidrap districts, as well as dwarf virus-infected plants from Sidrap and Wajo districts. Two healthy plant samples were used as a comparison. The SDS-PAGE technique was employed for the separation and analysis of proteins. The protein profile results in tungro-infected plants showed a protein band pattern between 31-116 kDa. Meanwhile, in dwarf virus-infected plants, a protein band pattern between 31-205 kDa was detected. These results indicate variations in protein expression between viruses and significant differences in protein profiles between tungro and dwarf virus-infected plants compared to healthy plants. Therefore, it is expected that this information can aid in designing more effective strategies for controlling virus infections in rice plants.
Potensi Aktivitas Antibakteri Minuman Fungsional Kombucha Berbahan Dasar Bunga Kecombrang (Etlingera elatior) Berdasarkan Lamanya Waktu Fermentasi Putu Rima Sintyadewi; Pande P Elza Fitriani; Ida Ayu Putu Ary Widnyani; Putu Indrayoni
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6126

Abstract

According to same reports, kombucha is a functional beverage that has the ability to boost immune system function by increasing T cells and containing antimicrobial substances that guard against microbial illness. The antibacterial properties of kombucha derived from kecombrang flowers have not been examined in a any of the numerous studies conducted on the beverage’s antimicrobial activity. The purpose of this study is to investigate how the lengt of fermentation effects the kecombrang flower kombucha antibacterial effectiveness. Fermentation times for the treatments were as follows (P1) 0 days, (P2) 3 days, (P3) 6 days, and (P4) 9 days. The commensal pathogenic microorganisms Escherichia coli, Salmonella typhimurium and Stapylococcus aureus were employed in this study. According to the research findings, the antibacterial activity of kecombrang flower kombucha was significantly impacted (P<0,01) by the duration of fermentation. The maximun antibacterial activity was found in kecombrang flower kombucha that was fermented for nine days (P4). It had an inhibitory power of 12,0 mm against Escherichia coli, 11,5 mm against Salmonella typhimurium, and 14,5 mm against Staphylococcus aureus. Kecombrang flower kombucha is known for its broad spectrum of antibacterial activity, which can suppress the growth of both gram-positif and gram-negatif pathogenic bateria. Based on these results, kecombrang flower kombucha has the potential to be an antibacterial agent and can functions as an alternative approach to functional drinks to control commensal pathogenic bacteria.
Potential Antioxidant Kombucha Fortified with Balinese Salak Juice (Salacca zalacca Var Amboinensis) From Karangasem District Ida Ayu Putu Ary Widnyani; Wahyu Krisna Yoga; Putu Rima Sintyadewi; Ni Komang Sri Ariani
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6128

Abstract

Salak (Salacca zalacca Var. Amboinensis) is widely spread in Indonesia. Processing salak using fermentation method can provide added value and extend the shelf life. Fermented salak product combined with kombucha from balck tea is expected to increase the product’s ability to inhibit free radicals and provide healthful effects for health, especially the digestive process. This research endeavors to assess the capacity and antioxidant activity (IC50) from the product. The data that has been obtained was analyzed statistically using a completely randomized two-factor design. The first factor involves the ratio of salak extract to black tea, featuring three levels 80:20 ; 70:30 ; 60:40. The second factor encompasesses varying fermentation duration with four levels 4,8,12, and 16 days fermentation. The experiment was replicated three times. The result is that the comparison of salak extract with black tea had an antioxidant capacity between 101,37-479,30 GAE/100g. The result for antioxidant activity (IC 50) between 97,77-179,61 ppm.
Effects of Land Application of Farm Dairy Effluent on The Growth of Sweet Corn Intan Nurcahya; Indra Permana; Erviyana Windiastuti; Rizki Nuralam
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6134

Abstract

This study investigates the impact of land application of farm dairy effluent (FDE) on the growth and yield of sweet corn (Zea mays var. saccharata). The agricultural sector faces increasing challenges in managing effluents sustainably, and the utilization of FDE as a potential nutrient source for crop production has gained prominence. Field experiments were conducted to assess the effects of FDE application on sweet corn with different rate of irrigation. Randomized completely block design was used an experimental design with different irrigation rate i.e. A = 0% FC effluent, B = 25% FC effluent, C = 50% FC effluent, D = 75% FC effluent, and E = 100% FC. The collected data was analyzed by using ANOVA with 5% of significance, a further test is performed using duncan multiple range test. The results showed that  FDE had TDS, TSS, BOD and COD higher than the standard with values ​​of 1398 mg/L, 2575 mg/L, 3611 mg/L and 6374 mg/L respectively. The application of FDE significantly influenced sweet corn growth especially on height and leaf increment. The application of FDE with 100% FC tends to have higher leaf increment than other treatments at 35 DAA (day after application).
The Influence of Different Media on The Physiological Response of Nila Fish (Oreochormis nilaticus) M. Ali Akbar; Sri Jayanthi; Samsul Fajri; Khairunnisa; Arini Shinta Zahara; Muliani Tiana Sari; Mardiah
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6135

Abstract

Nila fish (Oreochromis niloticus) is one of the leading commodities in freshwater fish cultivation in Indonesia is a freshwater fish commonly found in Indonesian waters. Media will be the main obstacle in cultivating tilapia on idle land (land that cannot be operated). Different water media influence the physiological responses of tilapia fish. The purpose of this activity is to determine the effect of differences in freshwater, brackish water and sea water media on the physiological response of tilapia fish. The parameters of this research are differences in water media, physical factors of the media, survival of tilapia fish, respiration rate of tilapia fish, mobility of tilapia fish, weight of tilapia fish and flexibility of tilapia scales. This study used quantitative methods with a completely randomized design (RAL). The collection technique makes observations. Data were analyzed using ANOVA with a significance level of 5%. The results of this study show that differences in the aquatic environment significantly affect the physiological response of tilapia. Where in sea water the fish mortality rate is quite high compared to fresh water and brackish water.
Identity of vertebrate diversity in Langsa City Forest Park Annisa Syabrina; Aura Alfisyahrin; Cut Anisa Khairani; Diah Khairani; Jeli Parsela; Syasya Salbila; Ayu Wahyuni
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6136

Abstract

Vertebrates is a type of animal that has a spine or backbone. Vertebrates are members of the phylum chordata and are bilateral animals (bilateral symmetry).This research aims to identify vertebrate species in the Langsa City Protected Forest which functions as ecotourism. In this research, the Exploration method using direct observation in the field has been analyzed descriptively. The research produced data that there were 22 vertebrate species in the Langsa City Protected Forest. Of the 22 species of vertebrates that are most protected in captivity, there are 3 species from the Acciptriformes order, 3 species from the Artiodactyla order. The results of this study are expected to provide information on the diversity of Vertebrata in the area of Langsa City Protected Forest that is used as an ecosystem.

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