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Jurnal SainHealth
ISSN : 25488333     EISSN : 25492586     DOI : -
Core Subject : Health, Science,
Jurnal SainHealth is a study journal in the field of science, health, and both of them. The journal is published by the Faculty of Health Sciences Universitas Maarif Hasyim Latif Sidoarjo.
Arjuna Subject : -
Articles 134 Documents
Kemampuan Enzim Bromelin Limbah Mahkota Nanas (Ananas comosus) dalam Menekan Pertumbuhan Pseudomonas aeruginosa Muhammad Sungging Pradana; Robiatul Muawenah; Evy Ratnasari Ekawati
Jurnal SainHealth Vol. 5 No. 1 (2021): Maret 2021
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v5i1.903.18-24

Abstract

Pseudomonas aeruginosamerupakan bakteri yang menginfeksi manusia yang sering ditemui pada kulit yang mengalami luka/trauma. Untuk penyembuhan luka infeksi menggunakan antibiotik tetapi banyaknya kasus resistensi terhadap antibiotik membuat angka infeksi semakin tinggi karena bakteri yang ada dalam tubuh semakin berkembang. Tujuan penelitian ini untuk mengetahui pengaruh enzim bromelin pada mahkota nanas (Ananas comosus) dalam menghambat pertumbuhan Pseudomonas aeruginosa. Metode maserasi digunakan untuk mengekstraksi mahkota nanas dengan pelarut etanol 96%. Ekstrak mahkota nanas diidentifikasi enzim bromelin dengan spektrofotometer serta dilakukan uji efektifitas enzim bromelin pada mahkota nans (Ananas comosus) dengan konsentrasi 25%, 50%, 75% dan 100% dalam menenkan pertumbuhan Pseudomonas aeruginosadengan metode difusi sumuran. Sebagai kontrol positif uji menggunakan Chloramphenicol dan sebagai kontrol negatif menggunakan larutan Pz yang sudah steril. Hasil kadar identifikasi enzim bromelin yang didapatkan pada mahkota nanas (Ananas comosus) adalah 0,153 U/mL. Uji daya hambat yang termasuk kategori sensitif hanya pada konsentrasi 100%. Hasil uji statistik menunjukkan terdapat perbedaan konsentrasi ekstrak mahkota nanas 25% dan 50% berbeda nyata dengan ekstrak mahkota nanas konsentrasi 75% dan 100%.
VERIFIKASI METODE UJI ANGKA ENTEROBACTERIACEAE PADA PRODUK SUSU DAN ANALOGNYA SESUAI SNI ISO 21528-2:2017 Fakhmi Rooslan Hamidi; Dian Indah Octavianingtyas; Inis Nadawati; Evy Ratnasari Ekawati
Jurnal SainHealth Vol. 7 No. 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.2068.1-8

Abstract

Enterobacteriaceae is a group of bacteria that inhabit the digestive tract of humans and animals, capable of causing nosocomial infections and potentially contaminating food products. Dairy products and their analogs are one of the routine food samples tested at the Testing Laboratory of the Balai Besar POM in Surabaya, whose quality and safety must be maintained for consumption by the public. One of the guarantees for the quality of test results is a test method that has been validated and verified. In this study, verification of the Enterobacteriaceae number test method was carried out with reference to SNI ISO 21528-2:2017. The results of the method verification were declared eligible, for the accuracy parameter, which was 102,26%, while for the precision parameter, it was 0,71% I. the sample group that was given the contamination (S+C) and 0,91% in the positive control group (K). It that be concluded that this method can be used in the Testing Laboratory of the Balai Besar POM in Surabaya.Keywords: Method verifications, Enterobacteriaceae, dairy products and their analogs, accuracy, precision.
Uji Potensi Antibakteri Ekstrak Kulit Jeruk Nipis Citrus aurantifolia (Christm) Swingle Terhadap Vibrio sp. Prasetya, Indra Adi Wira; Aziz, Aziz; Ekawati, Evy Ratnasari; Pradana, Muhammad Sungging; Batati, Nadiah Al; Pratiwi, Esti Rizkiana
Jurnal SainHealth Vol. 7 No. 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.6731.9-14

Abstract

Lime (Citrus aurantifolia) is one of the herbal plants that is rich in benefits, especially as a herbal medicines. Besides of lime fruit, lime peel also has antibacterial properties such as essential oils which contain flavonoids which can inhibit bacterial growth. This research utilizes lime peel which will be extracted as a test material. The purpose of this research is to determine the antibacterial potential of lime peel extract against Vibrio sp. at various concentrations. This research used the well-diffusion method using MHA as a test medium to determine whether lime peel extract can inhibit the growth of Vibrio sp. Various concentrations and penicillin as a positive control used in this research to determine which concentration has a greatest potential for inhibiting the growth of Vibrio sp. Result of this study used qualitative analysis by comparing the clear zone size produced on the medium. The obtained result shown that 20%, 40%, 60%, 80% and 100% concentration of lime peel can forming a clear zone which mean they can inhibit the growth of  Vibrio sp. The best concentration used to inhibit Vibrio sp. is 100% concentration which produce 23.7 mm clear zone on MHA medium. Lime peel extract can be used to inhibit Vibrio sp. growth based on the research result.
Perbandingan Metode Membran Filter dan Metode Tabung Ganda Terhadap Kandungan Escherichia coli pada Air Bersih Lathifa Azkhiyati; Dheasy Herawati; Setyo Dwi Santoso; Esti Rizkiana Pratiwi; Elsa Mega Suryani
Jurnal SainHealth Vol. 7 No. 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.6732.15-21

Abstract

Escherichia coli bacteria are bacteria used as indicators of contamination. Bacteriological water testing can use a Membrane Filtration (MF) and a Multiple Tube-Fermentation (MTF). This study aims to compare the membrane filtration and Multiple Tube-Fermentation methods on the presence of Escherichia coli in clean water. The two methods are compared to determine the percentage difference in the results of the two tests. The research methodology uses a description of observation. 20 samples of water boreholes were used and tested for the presence of Escherichia coli. Calculation of bacterial groups uses SNI standard references regulated by the Minister of Health of the Republic of Indonesia. The results showed that the average number of  Escherichia coli bacteria by the Multiple Tube-Fermentation method was less than the Membrane Filtration method. There is a difference in the percentage of the results of the two tests by 40%. Multiple Tube Fermentation is more sensitive than the Membrane Filtration method for detecting the presence of Escherichia coli in clean water.Keywords : Water, Escherichia coli, Membrane filtration, Multiple tube-fermentation
Uji Potensi Ekstrak Daun Sirsak (Annona muricata linn) Dengan Minyak Zaitun Terhadap Daya Bunuh Kutu Rambut (Pediculus humanus capitis) Muhammad Sungging Pradana; Titania Wahyu Eka Dinata; Abdul Chamid
Jurnal SainHealth Vol. 7 No. 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.6845.22-27

Abstract

Head lice (Pediculus humanus capitis) commonly infecting children in most countries. Soursop leaves (Annona muricata Linn) contain acetogenin compounds. The acetogenin compound functions as an anti feedent, some people also use olive oil to kill head lice, because olive oil contains flavonoids. The purpose of this study was to see the potential of soursop leaf extract with olive oil to kill head lice. This type of research is experimental. The method used to obtain soursop leaf extract is the maceration method. The results of giving soursop leaf extract (Annona muricata Linn) with olive oil (Olea eruopaea) on the mortality of head lice (Pediculus humanus capitis) in controls showed no dead head lice, 10% concentration of 4 dead head lice, 20% concentration 6 dead head lice, 30% concentration of 8 dead head lice observed every 15 minutes. Based on these results, it can be seen that the combination of soursop leaf extract and olive oil has the potential to kill head lice.Keywords: Soursop leaf extract, olive oil, head lice
Pemeriksaan Basil Tahan Asam dan Laju Endap Darah Sebagai Indikator Keberhasilan Pengobatan Penderita Tuberkulosis Paru di Puskesmas Singotrunan Banyuwangi Nadiah Al Batati; Dinata Muthmainnata; Evy Ratnasari Ekawati; Setyo Dwi Santoso; Elsa Mega Suryani; Indra Adi Wira Prasetya
Jurnal SainHealth Vol. 7 No. 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.6846.28-34

Abstract

This research is based on Mycobacterium tuberculosis infection which can increase the value of erythrocyte sedimentation rate. Where the transmission of these bacteria through the respiratory tract is known as Droplet Infection. The purpose of this study was to find out the effect of one month tuberculosis treatment on the erythrocyte sedimentation rate (ESR) and acid fast bacilli (AFB) examination. This type of research is a laboratory observation study with an accidental sampling technique of 30 samples which was carried out at the Singotrunan Banyuwangi health center for 30 days. The results showed that out of 30 samples examined, it showed that there was an influence of ESR and acid fast bacilli examinations on Tuberculosis patients who had been treated for one month. The conclusion is that there is an influence on Tuberculosis patients on ESR and AFB examinations at the Singotrunan Banyuwangi Health Center. Keywords : Tuberculosis, ESR, AFB
Pengaruh Ekstrak Ethanol dan N-Hexan Daun Serai (Cymbopogon citratus) Terhadap Larva Nyamuk Aedes aegypti Gland Konay Octavianus; Abdul Chamid; Dheasy Herawati
Jurnal SainHealth Vol. 7 No. 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.6847.35-40

Abstract

Dengue hemorrhagic fever (DHF) is a disease transmitted by the bite of a female Aedes aegypti mosquito infected with the dengue virus. The essential oil contained in lemon grass has antioxidant activity and contains active compounds that are toxic as larvicides. The purpose of this study was to determine the effectiveness, and the effective concentration of the Ethanol and N-Hexan extracts of lemon grass (Cymbopogon ciratus) leaves. The research subjects were 300 instar III Aedes aegypti larvae containing 25 larvae at each concentration with each concentration group namely 30 ppm, 35 ppm, 40 ppm, 45 ppm, and 50 ppm, which were observed for 1 hour, 3 hours, 6 hours, and 24 hours. From the results of the ethanol extract of citronella leaves (Cymbopogon ciratus) using graded concentrations Aedes aegypti larvae can die at high concentrations of 50 ppm and can kill 14 Aedes aegypti larvae. Whereas in the N-Hexan extract of lemongrass leaves (Cymbopogon ciratus) using graded concentrations Aedes aegypti larvae can die at high concentrations of 50 ppm which can kill 11 Aedes aegypti larvae. Lemongrass leaves (Cymbopogon ciratus) are effectively used as natural larvicides. Effective concentrations are 35 ppm, 40 ppm, 45 ppm, 50 ppm for 24 hours. Keywords: Aedes aegypti, Dengue Hemorrhagic Fever, Lemongrass (Cymbopogon ciratus) leaves.
Analisa Perbandingan Hasil Trombosit Pada Alat Hematologi Analyzer Metode Impedance dan Metode Optik Pada Kasus Anemia Hipokrom Mikrositer (Berdasarkan Indeks Eritrosit) Maulina Rosida; Budiono Raharjo; Halik Wijaya
Jurnal SainHealth Vol. 7 No. 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.6848.41-46

Abstract

Platelets have an important role in hemostasis, namely the formation and stabilization of platelet plugs. There are several methods in the counting of platelets. They can be carried out in a direct and an indirect manner. The direct method can be carried out in an automatic and automatic way. The automatic method uses an hematology analyzer which functions to measure and examine blood cells in blood samples. Counting the number of platelets is done automatically by two methods, namely optical and impedance which have different principles. The optical method is based on cell size and morphology, while impedance is based on cell size only. Hypochrome is a condition where the Hb concentration is less than normal so that the central achromia widens (> 1/3 of the cell) and there is a decrease in the color of the erythrocytes, namely an increase in the diameter of the central pallor above normal so that it looks paler. Microcyte is a condition where the cell size is smaller than normal. The purpose of this study was to determine the difference in the number of platelets in the hematology analyzer using the impedance method and the optical method in the case of microcytic hypochromic anemia (based on erythrocyte index) with a descriptive analytical research design. The test used is the paired t-test with a total sample of 30 samples. The results of platelets using the impedance method have an average of 339.37 * 103 / uL with a standard deviation of 120.294. The results of platelets using the optical method have an average of 314.70 * 103 / uL with a standard deviation of 113.569. A significant value of 0.000 significant value of (sig. < 0.05) can be concluded that there is a difference between the results of platelets on the hematology analyzer using the impedance method and the optical method in cases of microcytic hypochromic anemia (based on erythrocyte index). Keywords : Trombosit, Hipokrom Mikrositer, Hematology analyzer, Impedance, Optik.
ANALISIS KADAR VITAMIN D PADA PENERIMA VAKSIN SINOVAC SEBAGAI GAMBARAN SISTEM IMUNITAS Siti Nur Husnul Yusmiati; Aprilia Aly Damayanti; Christina Destri Wiwis Wijayanti; Tonny Watoyani
Jurnal SainHealth Vol. 7 No. 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.14321.47-50

Abstract

The body's immune system is a system that detects a wide variety of outside biological influences, organisms will protect the body from infections, bacteria, viruses to parasites. During the current pandemic, the immune system is very important to prevent exposure to COVID-19. Efforts are made to be able to sufficient intake of vitamins and vaccinations. Vitamin D is one that is needed to prevent exposure to viruses. This study aims to determine vitamin D levels in sinovac vaccine recipients as an illustration of the immune system. This scientific paper research uses primary data analytic observation method with a cross-sectional design supported by literature studies, namely by describing the results of the research in the form of a percentage (%) decrease and increase in vitamin D compared to normal values. The results of the research on vitamin D levels receiving the Sinovac vaccine found that 92% or 23 patients out of 25 patients showed vitamin D levels were still within normal values and 8% or 2 patients showed vitamin D levels below normal values. It can be concluded that some of the research samples were in the normal range so that the immune system as the response of vitamin D to vaccines is still being seen. Keywords : SINOVAC Vaccine, Vitamin D, Immune System.
PENGARUH VARIASI WAKTU SENTRIFUGASI NILAI HEMATOKRIT MENGGUNAKAN METODE MIKROHEMATOKRIT DI UNIVERSITAS BINAWAN Nurcholis Majid; Sabarina Elfrida Manik; Intan Kurniawati Pramitaningrum
Jurnal SainHealth Vol. 7 No. 2 (2023): September 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i2.14334.51-57

Abstract

Hematocrit is the total volume of red blood cells in 100 ml of blood expressed as a percentage of blood volume. Hematocrit examination is needed to help diagnose diseases such as anemia and dengue hemorrhagic fever (DHF) to determine the total volume of red blood cells or erythrocytes. The normal value for hematocrit examination is 40-54% for males and 37-47% for females. The purpose of this study was to determine the effect of variations in centrifugation time on hematocrit values using the microhematocrit method. This type of research is quantitative using experimental method with cross sectional design. The method used for hematocrit examination is the microhematocrit method with variations in centrifugation time of 2 minutes, 3 minutes, 4 minutes, and 5 minutes as a control with a speed of 12,000 rpm. The respondents of this study were 35 respondents and the samples of this study were 35 venous blood samples taken from Binawan University students, Medical Laboratory Technology Study Program batch 2021 and 2022. The average result of the 2-minute centrifugation hematocrit value examination is 37.87%, 3-minute centrifugation is 37.57%, 4-minute centrifugation is 37.30%, and 5-minute centrifugation is 37.25%. The Wilcoxon test results show the Sig. (2-tailed) of 2-minute centrifugation compared with 5 minutes is 0.00 (<0.05), 3-minute centrifugation compared with 5 minutes is 0.001 (<0.05), 4-minute centrifugation compared with 5 minutes is 0.560 (>0.05). The results of this study showed that hematocrit values with centrifugation times of 2 minutes and 3 minutes compared to 5 minutes had significant differences in hematocrit values.

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