Biosaintifika: Journal of Biology & Biology Education
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
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<![CDATA[Plant Response to Environmental Gradient Mediated by Trait Through Ontogeny on Common Tree Species at Two Contrasting Habitats in Karst Forest of Southern Taiwan ]]>
<![CDATA[Abdullah, Muhammad]]>;
<![CDATA[Sun, I Fang]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.8211
<![CDATA[One of central goal in ecology is to understand how plants respond to environment and what kind of attributes that can be obtained with an easy way to interpret the complexity of nature, especially on vegetation response. Ecologists use functional traits to understand how plants respond to environmental changes. Plant species may have experienced different environmental conditions during their ontogeny. Thus, they may show different patterns of ontogenetic trait variation (OTV) as their response to different environmental condition. In this study, the relationship between trait variation on different habitat and across ontogenetic stages both on community and population levels were investigated. Five selected leaves traits (leaf area, specific leaf area, leaf thickness, leaf dry matter content, and leaf succulence) were examined to look at plant response along soil water content and light gap interception gradient in Kenting forest dynamics plot (KFDP), Southern Taiwan. Leaf area was the most varied trait across habitat and ontogeny. Leaf thickness reveals an opposite pattern compare to leaf area. Leaf dry matter content (LDMC) showed less variation either between or within species and across ontogeny. Shift of community responses on environmental gradient by trait through ontogeny showed that intraspecific variation is important to be considered in ecological study. The other important finding in this study was by only using mean species we can misleading in understanding of plant responses to the environmental gradient in order to their adaptation both across different habitat and ontogenetic stages.]]>
<![CDATA[The Relationship of Body Length and Ratio Pappilla with Sex in Gobi Fish (Sicyopterus macrostetholepis) ]]>
<![CDATA[Sari, Rona Taula]]>;
<![CDATA[Zakaria, Indra Junaidi]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.8747
<![CDATA[Research about the relationship of body length and ratio papilla with sex in goby fish (S. macrostetholepis Blkr.) has been done at Animal Structure and Developmental Laboratory, Biologi Department, Faculty of Matematics and Natural Sciences, Andalas University, Padang, which purposed to analyse the relationship of body length and ratio papilla with sex of goby fish (S. macrostetholepis Blkr.). The samples were taken in wild stream area at Batangkuranji river, Padang City. This research used descriptive method and data were analyzed by qualitatively and quantitatively. The results of investigation showed that in several goby fishes (S. macrostetholepis Blkr.) with different sex had the same of body length and the same of ratio papilla. So, there was not relationship between of body length and ratio papilla with sex. Goby fishes (S. macrostetholepis Blkr.) it belongs to the hermaphrodite protogini, which the androgynous young females, while in adulthood, it would change sex to male. The results of this study are expected to add to the treasures of knowledge and information about reproductive gobies (S. macrostetholepis Blkr.) in the preservation and development of fish farming. ]]>
<![CDATA[Identification of Soybean Genotypes for Pod Shattering Resistance Associated with Agronomical and Morphological Characters ]]>
<![CDATA[Krisnawati, Ayda]]>;
<![CDATA[Adie, Mochammad Muchlish]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.8722
<![CDATA[A yield loss caused by pod shattering is one of the obstacles to the improvement of soybean productivity in tropical areas. The aim of this study was to identify the resistance of soybean genotypes to pod shattering as affected by agronomical and morphological characters. The field study was conducted in Malang, Indonesia, using 150 soybean genotypes. Data were collected on agronomical traits, the percentage of pod shattering, and pod morphological traits. Identification for shattering resistance was done as per oven dry method. Percentage of pod shattering was ranged from 0 % up to 100 % shattering with a mean of 58.11 %. Pod shattering was found to be negatively correlated with a number of pod per plant, the thickness of the pod, and Y/Z (seed weight and pod weight ratio). The Identification obtained 66 very highly susceptible genotypes, 19 susceptible genotypes, 19 moderate genotypes, 38 resistant genotypes, and 8 very resistant genotypes. Two of eight very resistant genotypes (G511H/Anj//Anj///Anj////Anj-6-11 and G511H/Anj//Anj///Anj////Anj-5-4) have high yield, medium maturity day and large seed size. Those lines could be used as gene donor for soybean varietal improvement for shattering resistance, and recommended to propose as new improved soybean varieties resistant to pod shattering in Indonesia.]]>
<![CDATA[Study of Mistletoe in Joben Resort Forest Mount Rinjani Lombok ]]>
<![CDATA[Fikriani, Wahyuni Dwi]]>;
<![CDATA[Mulyaningsih, Tri]]>;
<![CDATA[Aryanti, Evy]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.8427
<![CDATA[Mistletoes are one group of hemiparasite plants, including the Lorantaceae family that have potential as medicinal. These hemiparasite plants can attack flowering plant (Magnoliophyta) and non-floweing plant (Pinophyta), especially on the main stems, branches and twigs. The objective of this research is to identify the species of mistletoe and its hosts, make identification key, descriptions, and to make a distribution map of mistletoe in Joben Resort forest south of Mount Rinjani Lombok. This study is descriptive explorative research with three kinds of collecting sample methods i.e exploration, continous strip sampling, and delenation method. The research found five species of mistletoes are included in three genera i.e Amyema cuernosensis, Amyema enneantha, Amyema tristis, Macrosolen retusus and Scurrula artropurpurea. These five kinds of mistletoe are associated with 23 hosts species of plants, 18 genera from 13 families. The most favorite host of these mistletoes is Ficus septica, and the most agresive mistletoe is Scurrula artropurpurea. The important finding of the research is finding new species or new record of mistletoes. The benefit of these new record or new species is providing new material of new medicinal for treating some diseases such as various cancers.]]>
<![CDATA[Chromium Phytoremediation of Tannery Wastewater using Ceratophyllum demersum ]]>
<![CDATA[Suryani, Yani]]>;
<![CDATA[Cahyanto, Tri]]>;
<![CDATA[Sudjarwo, Toni]]>;
<![CDATA[Panjaitan, Diana Vici]]>;
<![CDATA[Paujiah, Epa]]>;
<![CDATA[Jaenudin, Mohamad]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.8716
<![CDATA[Tanning industry produces liquid waste containing heavy metals, especially chromium harmful for ecosystems and human health. Phytoremediation is a technique that utilizes the physiologicalpotential of plants to transform contaminants to be less or non-hazardous. The aim of the research is to determine the efficiency of Ceratophyllum demersum L. to remediate chrome in tannery wastewater. The research used 84 strands of C. demersum compound leaves of 30 g wet weight. The treatments consist of the use of 7.74 mg/L, 11.30 mg/L, 17.00 mg/L and 23.73 mg/L concentrations of chromium and a control. The research was conducted using a static method. The design of the experiment was the complete random design with 3 replication of treatment in 14 days. The parameters observed were the efficiency of chromium phytoremediation, water turbidity, BOD, and total chlorophyll level of the leaves. The results showed that the highest efficiency was at the concentration of 7.74 mg/L with 1.7% chromium, 17.3% water turbidity, and 46% BOD. Meanwhile, the highest efficiency of total chlorophyll level was 3.88 mg/L, reached at the concentration of 17.00 mg/L. In conclusion, C. demersum is good to use as a phytoremediator of tannery wastewater at the concentration of 7.74 mg/L, subsequently, these results can be used as a basis for the consideration of the application implementation in the process of liquid waste reduction.]]>
<![CDATA[Ethnobotanical Study of Medicinal Plants in Karangwangi, District of Cianjur, West Java ]]>
<![CDATA[Malini, Desak Made]]>;
<![CDATA[Madihah, Madihah]]>;
<![CDATA[Kusmoro, Joko]]>;
<![CDATA[Kamilawati, Fitri]]>;
<![CDATA[Iskandar, Johan]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.5756
<![CDATA[The knowledge and usage of plant as medicinal remedy by current generation are not as extensive as previous; therefore, many rural communities with restricted modern medical access still rely on traditional medicine. This paper provides significant ethnobotanical information on medicinal plants in Karangwangi Village of Cianjur District, West Java Indonesia. This study aimed to identify plants collected for medical purposes by the local people as well as to document the local names, uses, preparation, and location of these plants. Ethno botanical data was recorded by opting people participation and key informant approach involving semi-structured interviews, group discussions and filling of questionnaires. The results showed a total of 114 medicinal plants belonging to 50 families were identified. Zingiberaceae was the most-frequently cited (nine species), followed by Asteraceae, Euphorbiaceae, and Fabaceae (seven species each). The most-used plant parts were leaves (51.8%), followed by stems (22.9%) and the most common preparations were decoction, poultice and squeezed. Most of the plants were obtained from the house-yard and total of 30 medicinal uses were recorded. The ethnobotanical result documented in this study showed that this area is rich in medicinal plants and these plants are still commonly used for medicinal purposes among the people in their daily lives. Ethnobotanical heritage should be preserved, however, there is a gradual loss of traditional knowledge about these plants in new generation. Further, the findings can be used as baseline information for further scientific investigation for analyzing phytochemical, pharmaceutical and other biological activities for future drug discovery.]]>
<![CDATA[The Expression of mRNA LMP1 Epstein-Barr Virus from FFPE Tumour Biopsy: a Potential Biomarker of Nasopharyngeal Carcinoma Diagnosis ]]>
<![CDATA[Wahyono, Daniel Joko]]>;
<![CDATA[Gumilas, Nur Signa Aini]]>;
<![CDATA[Sulistyo, Hidayat]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.9028
<![CDATA[Nasopharyngeal carcinoma (NPC) is a multifactorial disease that is endemic geographically in the world. Indonesian population has a highly incidence rate that is 6.2/100,000 people year. The pathogenesis of NPC is more directly reflected by carcinoma-specific viral transcriptional activity at the site of primary tumour. Epstein-Barr virus (EBV) infection in NPC is reflected by the expression of EBV latent and lytic gene. In fact, mRNA Latent Membrane Protein 1 (LMP1) EBV expression was an important latent infection biomarker. The aim of this study was to determine a potential use of relative expression of mRNA LMP1 EBV from formalin-fixed paraffin embedded (FFPE) tumour biopsy in NPC as a tumour biomarker. This reseach design was a cross sectional study. The samples were the archived specimens of FFPE tumour biopsy from NPC WHO-3 patient which were collected from untreated patients from 2014 in the Department of Pathology Anatomy, Prof. dr. Margono Soekarjo Hospital, Purwokerto. The expression of mRNA LMP1 EBV expression was determined by RT-PCR technique. The positivity of mRNA LMP1 EBV expression was 51.9%, indicating a moderate positivity. The result proved that the expression of mRNA LMP1 EBV from FFPE NPC WHO-3 tumour biopsy was a potential biomarker of NPC diagnosis. The molecular methods would improved the management of NPC, particularly in the histopathological diagnosis of NPC.]]>
<![CDATA[Diversity and Distribution of Myrmecophytes in Bengkulu Province ]]>
<![CDATA[Safniyeti, Safniyeti]]>;
<![CDATA[Sulistijorini, Sulistijorini]]>;
<![CDATA[Chikmawati, Tatik]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.9285
<![CDATA[Myrmecophyte is a common medicinal plant used by local people in Indonesia for treating various diseases especially in Papua. Bengkulu province is one of the Myrmecophyte habitats, but there has no report on its identity and distribution. The objectives of this research were to identify the diversity and analyze the Myrmecophytes distribution as well as factor affecting its presence. This study used purposive sampling method by exploring the area where Myrmecophytes commonly found. The Myrmecophyte distribution based on host tree was analyzed using Morishita index and the autecological analysis of abiotic factors was performed using Principal Component Analysis (PCA) generated from Minitab 16. The results of this research showed that there were two species of Myrmecophytes in Bengkulu province, namely Hydnophytum formicarum and Myrmecodia tuberosa, as well as two variants of M. tuberosa i.e. M. tuberosa armata and M. tuberosa siberutensis. The distribution of Myrmecophytes based on host tree was mostly randomly scattered in Central Bengkulu regency, Seluma, North Bengkulu, South Bengkulu, and Kaur. Their distributions were affected by light intensity and temperature. The data of this research can be used as basic information for carried out conservation efforts in Bengkulu province. The abundance of Myrmecophytes is also used as a source of additional income for local people in Bengkulu province. ]]>
<![CDATA[Capability of Vitamin E as a Radioprotector in Suppressing DNA Damage Determined with Comet Assay ]]>
<![CDATA[Darlina, Darlina]]>;
<![CDATA[A., Lusy Dahlia]]>;
<![CDATA[Alatas, Zubaidah]]>;
<![CDATA[Kisnanto, Teja]]>;
<![CDATA[Syaifudin, Mukh]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.8333
<![CDATA[Radiation has a potent to damage cells. Radiation may act directly or indirectly on deoxyribonucleic acid (DNA) that results in the degeneration of tissues and necrotic, and thereby it needs a potent radioprotector to prevent these damages. Vitamin E is natural product known as an antioxidant which has potential as radioprotector. This research aimed to determine the capability of vitamin E with emphasized on the searching for its optimal concentration as radioprotector of DNA damage. This study used blood samples of healthy person irradiated with gamma rays at a dose of 6 Gy as the lethal dose to lymphocytes. The cocentrations of vitamin E from 0 to 0.8 mM was added into blood 15 minutes before irradiation. Isolation of lymphocytes was done using gradient centrifugation method. Evaluation on the capability of this compound in suppressing DNA damage was done by using alkaline Comet assay and data analysis was done using CaspLab program. The results show that addition of vitamin E could suppres these DNA damages and 0.8 mM of vitamin could reduce DNA damage up to 94.2%. We conclude that vitamin E effectively suppresed DNA damages induced by radiation. This information may benefit to the patient from negative impacts of radiotherapy.]]>
<![CDATA[Improving Post-graduate Students Learning Activities through Lesson Study in Learning Forest-Prototype ]]>
<![CDATA[Muhfahroyin, Muhfahroyin]]>;
<![CDATA[Oka, Anak Agung]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.6208
<![CDATA[Improving learning quality in 21st can not be separated from contextual learning and student-centered learning paradigm. The contextual lesson study program conducted in learning-forest prototype to build a learning community. The objectives of this research were to improve learning activities of postgraduate students in Biology Education department and to build a learning community. The implementation of lesson study was conducted in the Biology Learning Innovation subject for postgraduate students while practicing to observe open lesson in the undergraduate students which used learning forest-prototype. The postgraduate students took roles as planner, observer, and reflector in the plan, do (open lesson), and see (reflection) activities. The implementation was done in three cycles in even semester of academic year 2015/2016. Students learned collaboratively and contextually. The postgraduate students learning activities were observed by six observers from lecturer colleagues. The research results showed that the students were able to implement planning, open lesson, and reflection properly. The average of students learning activity grade was 91.11% from all of students, with the grade averages for planning, open lesson, and reflection activities were 88.89%, 93.33%, and 91.11% respectively. The implementation of this lesson study in the learning forest-prototype can be done in other relevant subjects to strengthen learning activities.]]>
