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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
 103   104   105   106   107 
Verification of Maman (Cleome gynandra (L.) Briq.) from Riau Based on matK and trnL-trnL-trnF Intergenic Spacer Roslim, Dewi Indriyani; Herman, Herman; Putri, Shalsadila Rahmadani; Furqoni, Aries Tri; Budiono, Deanne Yoshe Fidela; Altuhaish, Adeel Abdulkarim Fadhl; Lestari, Wahyu
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.46937

Abstract

Maman is a traditional plant frequently used as food by people in Melayu Rokan of Riau Province. Morphological detection indicates that the scientific name of maman is Cleome gynandra L.  The objective of this study is to confirm the taxonomic status of maman from Riau basedon matK and trnL-trnL-trnF intergenic spacer (IGS) sequences. Methods utilized in this study included sampling, DNA extraction, PCR, electrophoresis, sequencing, and data analysis. The fresh leaves were picked up from Rokan Hulu Regency, Riau Province. In this study, the matK and trnL-trnL-trnF IGS sequences of maman with a length of 754 bp and 938 bp, respectively, had been obtained. The BLASTn analysis based on both sequences showed that maman had 100% similarity to Gynandropsis gynandra. Cleome gynandra was synonymous with G. gynandra. There were 49 nucleotide variations, 16 critical nucleotides, and there were no indels in matK. Meanwhile, in trnL-trnL-trnF IGS there were 181 nucleotide variations, 13 critical nucleotides, and 61 indels. In conclusion, this study succeeded in confirming the taxonomic status of maman from Riau as C. gynandra syn. G. gynandra. The result enriched the abundance of DNA barcode sequences of this plant in GenBank which is useful for the molecular identification.
Potential of Therapeutic Curculigo latifolia Extracts on Alloxan-induced Diabetes in a Male Mus muscullus Haryanto, Haryanto; Sutandi, Agus; Kusumawati, Eni; Nurhayati, Sari; Fitri, Fadillya Maulidilla; Nafsi, Ginan; Nuraeni, Sri Wahyuni
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.40498

Abstract

Curculigo latifolia is a herbaceous plant that is abundant on the islands of Java, Sumatra and Kalimantan. C. latifolia has not been well explored. The research to determine the phytochemical content of C. latifolia, to analyze the organoleptic sweetness level of C. latifolia fruit and to analyze the anti-diabetic potential of C. latifolia plant extracts on  diabetic mice. The study was conducted experimentally using  6 treatment levels and 4 replications. Prior to treatment, mice were induced hyperglycemia using alloxan 150 mg/kg WB were induced subcutaneously. The treatment being tested was oral Ethanol crude extract (ECE) for 28 days with 400 mg/kg WB, namely: G1: oral mineral water; G2: glibenclamide ; G3: ECE leaf; G4: ECE root; G5: ECE fruit and G6: ECE tree. Blood sugar levels were measured at 0, 7, 14, 21 and 28 days after oral ECE. Therefore, respondents stated that after consuming the fruit, they had a sweet-tasting and taste-modifying mineral water with a strength of 82.40 ± 8.36%, but the fruit extract did not show any sweet-tasting and taste-modifying. ANOVA results showed that oral ECE administration had a significant effect low on blood sugar levels. the HSD test was carried out with a 95% confidence level. ECE C. latifolia showed positive results on the tests of flavonoids, phenolics, saponins, alkaloids, triterpenoids and tannins. G4 was effective in reducing sugar levels after short time and  G5 for 28 days (long time). C. latifolia have pharmacology effects to lower sugar levels and has taste modifying to sweetness.
Metagenomic Analysis of Microbial Communities from Coal Waste Elawati, Nunung Eni; Lestari, Catur Retno; Marco, Martha Aulia; Dewi, Sintia Puspa; Ramadan, Bimastyaji Surya
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.47865

Abstract

Coal waste contains few macro-and micronutrients, which makes it less likely to become a growth site for microorganisms. One way to screen coal waste resources is to identify the diversity of microbes in them to study the relationship between these microbial communities in contributing to improving environmental pollution using metagenomics to determine microbial diversity. The purpose of this research is to study the diversity of microorganisms in areas contaminated with heavy metals using a metagenomic method. This study was performed using next-generation sequencing techniques, including DNA extraction, 16S rRNA amplification, and gene sequencing analysis. The results of this research found that the 10 most commonly found species were Baekduia soli, Nocardiodes iriomotensis, Nocardioides mesophilus, Nocardioides pakistanensis, Propionibacterium cyclohexanicum, Solirubrobacter ginsenosidimutans, Gemmatirosa kalamazoonensis, Roseisolibacter agri, Kosakonia saccahri, and Dickeya fangzhongdai. Based on this research, it can be concluded that most of the microbial communities from coal waste are dominated by the phylum Actinobaceria. The results of this study can be used as an adaptive microbial germplasm for industrial waste management strategies.
The Improvement of Lipid Profiles and Glucose Resistance of Hypercholesterolemic Rats using Tempeh Flour Based-Yoghurt Complemented with Red Pitaya Peel Extract Nugraheni, Kartika; Putriningtyas, Natalia Desy; Fernando, G. S.N.; Bintari, Siti Harnina
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.47414

Abstract

Tempeh flour is potentially a substitute material in making yoghurt that provides bioactive for improving cardiovascular health. It is also suitably combined with antioxidant sources, such as red pitaya peel extract, which is well known for inhibiting metabolic inflammation. This study aims to analyze the effectiveness of tempeh flour-based yoghurt complemented with red pitaya peel extract in improving lipid profiles and glucose tolerance in hypercholesterolemic rats. A total of 36 male Wistar rats were used and divided into six different groups: regular diet (ND) and five high-fat-diet rat groups treated with no supplementation (HFD); diary-based yoghurt with lactic acid bacteria (YBAL); commercial yoghurt (YB); tempeh flour based-yoghurt with lactic acid bacteria (SY); and tempeh flour based yoghurt complemented with red pitaya peel extract (SDS). The supplementation of each treatment group was given a dose of 3.6 ml/ KgBW/ day as a single dose for 28 days. The blood sample was collected from sinus orbitalis and then used for lipid profile, glucose, liver enzyme analysis, and statistical analysis using ANOVA. The result shows that glucose levels increased significantly in all groups, except HFD groups, and there is no significant difference between normal and yoghurt treatment groups. However, yoghurt and red pitaya peel extract supplementation significantly decrease LDL-C and increase HDL-C levels while improving liver condition during hypercholesterolemia. In conclusion, tempeh flour-based yoghurt complemented with red pitaya peel extract can improve lipid profile and liver health in hypercholesterolemia rats. Therefore, further research on metabolism pathways is needed to develop potential nutraceutical products for people with hypercholesterolemia.
Spirulina Flour-based Feed Additive Improves the Physical and Chemical Eggs Quality of Tegal-Laying Duck Sunarno, Sunarno; Nur Handyka, Muhammad Ammar; Mulyani, Sri; Suparmi, Suparmi
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.48114

Abstract

Spirulina is a species of blue green algae that contains many phenolic compounds, phytonutrients and pigments which have potential as a feed additives to improve the physical and chemical quality of eggs. The present study evaluated the effect of spirulina flour-based feed additive supplementation during the late growth to the early egg-laying period on Tegal-laying ducks' physical and chemical egg quality. A total of 25 ducks were randomly divided into 5 treatments; the control group treated with standard feed (P0), P1, P2, P3, and P4 were treated with standard feed supplemented by spirulina flour-based feed additive at concentrations of 0.3%, 0.6%,1.2%, and 2.4%, respectively for four weeks. Physical quality indicators included the Haugh unit, yolk index, weight measurements, eggshell thickness, and shape index. Chemical quality indicators assessed albumen protein content, egg yolk cholesterol, and b-carotene levels. This research revealed that spirulina flour, added at concentrations between 0.30% and 2.40%, significantly enhanced the egg quality of Tegal-laying ducks compared to the control group. This study concludes that spirulina flour can be used as a feed additive to improve the physical and chemical quality of Tegal-laying ducks’ egg. The novelty of this research is spirulina flour has the potential to be used as a feed additive to increases the albumen protein levels, increases b-carotene, and reduces egg yolk cholesterol in eggs of Tegal-laying ducks. Spirulina flour can be a potential source of feed additive for various other local ducks in Indonesia.
Population Assessment of Sulawesi’s Endangered Snoring Rails (Aramidopsis plateni Blasius) in Gandang Dewata National Park Putera, Alexander Kurniawan Sariyanto; Nasir, Yusrianto; Rusmidin, Rusmidin; Nuruliawati, Nuruliawati; Berryman, Alex J.
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.47487

Abstract

This study focuses on assessing the critically endangered mandar dengkur, or snoring rails (Aramidopsis plateni Blasius 1886), within Indonesia's Gandang Dewata National Park. Despite its importance in regional biodiversity, the species remains poorly documented. The primary objectives of this research are to investigate the population and ecological role of the mandar dengkur in Gandang Dewata National Park. It aims to understand their population status, the factors influencing their survival, and the level of global threats facing this species. Field surveys from July to August 2023 employed point counts and interviews with local residents, revealing a population of 20 snoring rails. Tondok Bakaru was identified as the primary subpopulation, with the highest count and probability of occurrence. Tondok Bakaru, characterized by remote and isolated areas, plays a crucial role in snoring rails' survival, mirrored by discoveries of other sensitive species like the Anoa. Environmental variables analysis through Generalized Linear Modeling (GLM) identified the nearest food source and settlement as significant factors influencing snoring rail populations, aligning with their elusive nature. The selected GLM model highlighted the impact of average NDVI, distance to the nearest food source, and distance to the nearest settlement on snoring rail density, guiding conservation efforts. This study underscores the urgency of upgrade the snoring rails' conservation status to Endangered (EN) and implementing global conservation measures by improving data collection with technology.
Evaluating the Potential of Fermented Leguminous Plant Leaves as a Fertilizer for Rice (Oryza Sativa) Production Ibañez Jr., Roger Y.; Velza, Jacob Frederick P.; Pelayo Jr., Cristito C.
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.45521

Abstract

Synthetic fertilizers improved rice yield, but they were expensive and reduced soil health. Fermented leguminous plant leaves (FLPL) were natural fertilizers that had a lot of nutrients that plants needed to grow and develop because they were locally available and economical to farmers. This study investigated the effects of fermented leguminous plant leaves as fertilizer for rice. The four treatments were arranged in a Randomized Complete Block Design and replicated three times. Plant height, tillers, grain weight, and economic returns were collected and analyzed using Analysis of Variance (ANOVA). The results showed that the control group that received synthetic fertilizers had significantly (P0.05) taller rice plants compared to the treatment groups. However, among the fermented leguminous leaves, rice plants that received Madre de cacao showed the highest plant height due to its high phosphorus content. The study also found no significant differences (P0.05) in the number of tillers between the treatments, indicating that fermented leguminous leaves can provide the necessary nutrients for crop growth. It was concluded that fermented leguminous leaves can be used as fertilizer to promote eco-friendly rice farming practices. Madre de cacao leaves were recommended as organic fertilizer for rice for at least three months to enhance plant height and overall plant growth. The potential of FLPL, especially from madre de cacao, as an economical alternative to synthetic fertilizers is a breakthrough in rice production. The results demonstrated the potential benefits of using Madre de Cacao leaves as fertilizer to promote eco-friendly rice farming.
Tea Fungus Beverages from Torch Ginger (Etlingera elatior): Total microbial, Physicochemical, and Antioxidant Activity Fitrianto, Nur; Husen, Fajar; Samiyarsih, Siti; Ratnaningtyas, Nuniek Ina; Palindung, Lusia Seti; Azizah, Enur
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.47944

Abstract

Functional beverages are needed to maintain health and fitness as a part of the instant lifestyle and degenerative diseases. The scientific findings on torch ginger (Etlingera elatior) flower (TGF) as a tea fungus beverage (Kombucha) are still limited. This research evaluated total microbes, physicochemical properties, and antioxidant activity 7 and 14 days after fermentation (daf) of TGF kombuchas. In addition, TGF in variant 0% (TGF0), 5% (TGF5), 10% (TGF10), and 15% (TGF15) are fermented with 3% of SCOBY, 20% culture, 1% green tea, and 10% sucrose. The antioxidant activities of TGF kombuchas were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azino-bis 3-Ethylbenzothiazoline-6-Sulfonic Acid (ABTS) assay—the phenolic and flavonoid content using Folin-Ciocalteu method-gallic acid equivalent, and quercetin acid equivalent, respectively. The result showed that the fermentation time (p0.05) was affected by the properties of TGF kombuchas. Total microbes, physicochemical properties (pH, acidity, cellulose pellicle weight, phenolic, flavonoid content), and antioxidant activity were higher in 14 than in 7 days. Total soluble solid and total yeast count showed lower values in 14 days. The increase in total bacteria count, TGF15%, led to the highest increasing density from 8.08 ± 0.02Log CFU/mL on seven daf to 13.34 ± 0.04Log CFU/mL on 14 daf. The TGF 10% and 15% kombuchas in 14 dafs showed abundance in phenolic and flavonoid content, 121.45±1.07mg GAE/mL and 1.70±0.04 mg QAE/mL, respectively. During 14 daf and TGF 10%, the Kombucha of torch ginger flower demonstrated high antioxidant activity at 85.92±0.07% DDPH and 63.05±0.97ABTS. It is expected to aid future research into developing functional kombucha beverages.
Phylogenetic Analysis of Baccaurea Spp. in West Sumatra Using MatK Molecular Markers Saswita, Helvi Maudy; Syamsuardi, Syamsuardi; Nurainas, Nurainas; Suwardi, Adi Bejo; Taufiq, Ahmad
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.46051

Abstract

Baccaurea is a group of fruit-producing plants found in wild forests. Many of these plant species have not yet explored their potential. The high rate of deforestation in West Sumatra poses a threat of extinction to the genetic resources of the genus Baccaurea before being explored and identified. The research aims to analyze the sequence characters and phylogenetic of Baccaurea found in West Sumatra using the MatK molecular marker. Phylogenetic analysis using the Maximum Likelihood (ML) method in MEGA X application. The results of the analysis of the six species of Baccaurea found that the sequence length ranged from 854-1019bp, the percentage of G+C base composition is 33.4%, the percentage of A+T base composition is 66.6%, the genetic distance range is 0-4% with a conservative character of 484bp and informative characters of 4bp. While the phylogenetic analysis using the ML method grouping the six species of Baccaurea to form a monophyletic clade with a bootstrap value of 100%, all species collected were in the same clade. These results reveal the first time that the MatK sequences from six species Baccaurea native to West Sumatra will be included in NCBI for use by other studies in conducting broader phylogenetic research.
Cover and Table of Content Journal of Biology & Biology Education, Biosaintifika :
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.50269

Abstract

December 2023

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