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Biosaintifika: Journal of Biology & Biology Education

biosaintifika
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Published by Universitas Negeri Semarang

ISSN : 2085191X EISSN : 23387610 DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.

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Articles 1,068 Documents

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Studi Etnobotani Pengobatan Tradisional untuk Perawatan Wanita di Masyarakat Keraton Surakarta Hadiningrat Verary Shanthi, Rini; -, Jumari; Izzati, Munifatul
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Keraton Surakarta memiliki budaya pengobatan tradisional. Pengetahuan tentang tradisi tersebut tersimpan dalam naskah kuno. Seiring dengan kemajuan jaman dan perkembangan pengobatan modern, tradisi pegobatan tradisional mulai menurun. Tujuan dari penelitian ini, untuk mengkaji etnobotani pengobatan tradisional untuk perawatan wanita di Keraton Surakarta. Kajian penelitian meliputi keanekaragaman jenis tumbuhan obat komposisi dari ramuan tradisional dan mengkaji tingkat pengetahuan masyarakat Keraton Surakarta dalam penggunaan ramuan tradisional. Penelitian ini dilakukan di Keraton Surakarta dan kelurahan Baluwarti. Pengumpulan data etnobotani dengan wawancara, studi litelatur, survey, dan kuisioner. Data keanekaragaman jenis tumbuhan obat diperoleh dari kajian Serat Husada dan hasil wawancara dengan informan. Jenis ramuan tradisional, diulas mengenai komposisi bahan, organ tumbuhan, cara meramu, dan cara pemakaian. Data tingkat pengetahuan masyarakat diambil melalui kuisioner. Jumlah responden sebanyak 60 orang. Analisis data menggunakan Analysis of Variances (ANOVA). Hasil penelitian menunjukan 120 spesies tumbuhan obat dari 55 famili digunakan untuk ramuan tradisional. Terdapat 61 jenis ramuan yang digunakan untuk 17 macam perawatan wanita. Hasil kuisioner menunjukkan kecenderungan menurunya tingkat pengetahuan dan penggunaan ramuan tradisional oleh wanita usia muda. Hal ini karena pengaruh kemajuan jaman dan perkembangan pengobatan modern.Surakarta Palace develops its own traditional medication culture. The knowledge of the tradition is well-preserved in ancient manuscripts. In line with the modern medicine development, the practice of traditional medical treatment has declined. And therefore the aim of the current study was to evaluate the traditional medical treatment for women in Surakarta Royal Palace. The purpose of the study was to examine the diversity of medicinal plant, the composition of traditional ingredients, and the knowledge level of members of Surakarta Royal Palace. The study was conducted in Surakarta Royal Palace and Baluwarti Village. Ethnobotanical data were collected through interview, literature study, survey and questionnaire. Data of the medicinal plant diversity were accumululated from the study of Serat Husada and from interviews with the informants. The traditional ingredients were reviewed in terms of the composition, the plant organs to be used, how to mix the materials, and how to consume them. Data of the level of public knowledge were taken through questionnaire. There were 60 respondents of the study. Data were analyzed using ANOVA. Research revealed that 120 species of medicinal plants from 55 families were utilized as traditional medicine. As many as 61 medicinal plants were used in 17 types of women treatment. The study also suggested that the knowledge level and the utilization of traditional medicine were decreasing among young women. It was concluded that the modernization of medicinal world has been the main cause of this decrease.

Isolation and Identification of Î±-glycosidase Inhibitor Producer Endophytic Bacteria from Salak Pondoh Fruit (Salacca edulis) Susilowati, Ari; Dewi, Citra Praba Yunita; Sari, Siti Lusi Arum
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.21031

Alpha-glycosidase inhibitors can delay the hydrolysis of oligosaccharide and disaccharide into glucose, which can prevent or treat hyperglycemia in diabetes mellitus. The rind and flesh of Salak Pondoh fruit are known to produce ?-glycosidase inhibitor compound. Endophytic bacteria that live in plant tissues potentially produce compounds such as in host plants. Exploration of endophytic bacteria from the rind and flesh of Salak Pondoh is one of the efforts to obtain isolates of bacteria producing ?-glycosidase inhibitors. The objective of this study is to isolate and identify endophytic bacteria producing inhibitor ?-glycosidase from rind and flesh of Salak Pondoh, and to know the activity of its ?-glycosidase inhibitor. Isolation of endophytic bacteria was done by inoculating surface-sterilized plant samples on Nutrient Agar (NA) medium. The inhibitory activity towards ?- glycosidase analysis was performed using the spectrophotometric method (? = 415 nm), with p-nitro phenyl ?-D-glucopyranoside as the substrate. Identification of the bacteria was performed based on the 16S rRNA gene sequence. The sequencing was done at 1st Base Singapore and the obtained sequences were identified using the BLAST Nucleotide device on the NCBI website. In total, 6 bacterial isolates were obtained. The inhibitory activity ranged from 6.14-62.95% and the highest was generated by Kt-E isolates. The bacteria obtained were isolated Dt- A and Dt-B that represent genus Xanthomonas, Kt-E from genus Paenibacillus, Kt-I from genus Bacillus, Dm-A1 and Dm-A2 from family Enterobacteriaceae. The results confirm the potential of the endophyte bacteria of Salak Pondoh to be an alternative source of hyperglycemia medication.

APPLICATION OF RICE ROOT ENDOPHYTIC BACTERIA IN CIHERANG VARIETY RICE (ORYZA SATIVA) SEEDS Fitri, Lenni; Ismail, Yulia Sari; Putriani, Putriani; Warzatullisna, Warzatullisna
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.21697

Endophytic bacteria are microorganisms that thrive in plant tissue without causing any negative effects to the host plants. The purpose of this study was to apply endophytic bacteria that have the potential to produce IAA hormones as biological agents in rice seeds. This research was conducted by testing IAA hormone using a spectrophotometer, testing HCN production using filter paper, biochemically identification of the isolate, and applicating endophytic bacteria into rice seeds. Endophytic bacteria have different abilities in producing IAA hormone. Based on the results of the study, the highest IAA concentration (57 ppm) was obtained from KSB1 (Potassium Solubilizing Bacteria) isolates and followed by KSB3 isolate with 56 ppm. All the endophytic bacteria isolates were unable to produce HCN. Results of the study showed that KSB1 and KSB3 isolate were able to influence the growth of root length by 5.62 cm and 6.70 respectively as well as the canopy height by 8.56 cm and 8.30 cm respectively. Based on observations of colony morphology, cell morphology and biochemical tests, KSB1 was presumably belong to the genus Staphylococcus. In this study, new information about endophytic bacteria that can increase the growth of Ciherang variety rice can be obtained. This study provides information that the application of environmentally friendly bacteria is important to preserve the environment and agriculture sustainability in Indonesia without reducing the productivity of rice.

IDENTIFYING THE POTENTIAL YIELD OF SOYBEAN GENOTYPE IN TIDAL LAND TYPE C Susanto, Gatut Wahyu Anggoro; Putri, Pratanti Haksiwi
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Identification of soybean genotypes including potential yield character is a base to understand soybean growth, development, and productivity on potential lands. The purpose of this research is to determine the potential yield of soybean genotype in tidal land type C and their supporting components. Four soybean genotypes and two comparative varieties Anjasmoro and Lawit (adaptive tidal land) were tested on tidal land type C at four locations (three locations in Jambi Province and one location in South Kalimantan Province), July 2017. The research was conducted using a randomized design for each location and repeated four times. The plot size was 12 m2, spacing row 40 cm x 15 cm, two plants for each hole. GH_KR_13, GH_GR_1 and GH_GR_5 productivity equal with Lawit, the adaptive variety for tidal land type C. While, Anjasmoro showed more than 2.20 t/ha in seeds productivity, 54 filled pod for each plant, with large size of seeds (15.84 gram/100 seeds). Anjasmoro had higher seed productivity than all of genotypes including Lawit. Anjasmoro with it?s potential yield and productivity, can become farmer preferences and deserve to be developed in tidal land type C.

PHYCOCYANIN FROM SPIRULINA PLATENSIS INDUCES CYTOTOXICITY AND APOPTOSIS IN T47D CELLS Dimarti, Safira Chairani; Susilaningsih, Neni; Yuniati, Renni
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Breast cancer cases in Indonesia have risen in alarming rate. Herbal medicines have been developed to complement conventional cancer therapy and to lessen the negative effects of the therapies. Phycocyanin, blue-green photosynthetic pigment from Spirulina platensis, becomes a potential candidate of herbal medicine for cancer therapy due to its capability to eliminate various cancer cell line on in vitro level. This research aimed to analyse the effectivity of phycocyanin to induce cytotoxic and apoptotic activity of T47D cells (breast cancer cell line). MTT assay was perform to measure IC50 value. Double-staining assay and flow cytometry were applied to visualize the appearance of apoptotic cells and to measure the percentage of apoptotic and necrotic cells. Result showed that the IC50 value of phycocyanin towards T47D cells is 1650 ?g/mL. Phycocyanin was also proven to induce apoptosis at IC50 with the apoptosis percentage of 45.93%. This research presented different results from previous researches that used phycocyanin from saltwater Spirulina platensis. Based on the results of this study, phycocyanin has the potential to be developed as anticancer agent by inducing apoptosis.

GENETIC DIFFERENCE BETWEEN TWO PHENOTYPICALLY SIMILAR MEMBERS OF ASTERACEAE BY THE USE OF INTERGENIC SPACER ATPB â€“ RBCL Susanto, Agus Hery; Dwiati, Murni
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Two Asteraceae species, i.e. Synedrella nodiflora (L.) Gaertn and Eleutheranthea ruderalis (Swartz) Sch.-Bpi. are phenotypically similar with each other, although some differences in morphological and anatomical traits are apparently observable. Molecular comparison using particular marker is required to support a phenotype-based study that previously reported. Chloroplast DNA marker, . atpB ? rbcL IGS, was used to identify genetic difference between both species. Six samples of the respective species were collected randomly from some places in Banyumas Regency, Central Java, Indonesia. Amplification of the marker was performed employing a pair of universal primers. Sequence alignment on the PCR products showed that no difference in atpB ? rbcL IGS sequences, either within S. nodiflora or E. ruderalis samples was observed. On the other hands, several deletions and base substitution in both S. nodiflora and E. ruderalis were detected when alignment was made between both species. This result suggests that they reveal a convincing genetic difference. Inspite of no direct correlation between this genetic and some visible phenotypic differences, this finding provides preliminary scientific background on the phenotypic traits of both species, which are often difficult to find at a rapid observation.

INDUCTION AND GROWTH KINETICS CALLUS OF TOMATO (SOLANUM LYCOPERSICUM) Setiaji, Arkan; Annisa, RR Rifka; Rumiyati, Rumiyati; Semiarti, Endang
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.21704

Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ?Permata?) for optimum callus induction. Seeds were grown on ¼ MS medium for 10-15 days. Hypocotyl was excised and cultured on MS medium + 2 mg/l 2,4-D for 15 days as the explants for callus induction. Callus was transferred to MS medium with 8 variations of PGRs including the combination of BAP + NAA, and 2,4-D. Both fresh and dry weight was measured every 5 days over 60 days to establish the growth kinetics and growth efficiency of callus. Anatomic characters of calli were examined through paraffin-embedded method. The result showed of MS medium supplemented with 2.0 mg/l NAA and 0.2 mg/l BAP is optimum for tomato callus induction, based on highest number of the absolute growth rate on fresh weight (73.77% per day), dry weight (3.84% per day), and callus initiation time (5.56 days) achieved by the medium. Cells in the ground tissue of tomato hypocotyl are competent to be dedifferentiated into a callus. This research results were expected to find out suitable methods for tomato callus production in preparation for skincare uses.

PRODUCTION OF CONIDIA BY ENTOMOPATHOGENIC FUNGI AND THEIR PATHOGENICITY AGAINST COPTOTERMES SP. Zulfiana, Deni; Zulfitri, Apriwi; Lestari, Anis Sri; Krishanti, Ni Putu Ratna Ayu; Meisyara, Dita
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22435

Entomopathogenic fungi have the potential to infect most arthropods including termites which are economically important major insects pest of wood, wood products and building structures. However, the application of this fungus in the field has not shown satisfactory results yet, one of which is constrained in mass production of conidia. The purpose of this study was to evaluate 16 types of biodegradable products and waste as substrates for mass production of conidia using solid state fermentation method and two types of inoculum namely solid and liquid inoculum. Toxicity tests were carried out on subterranean termites (Coptotermes sp.) based on JIS K 1571, 2010. The parameters observed were the number and dry weight of the conidia produced, conidial viability, nutrient content of the substrate, and percentage of termite mortality. The results showed that rice, sorghum and corn were the best media for the growth of entomopathogenic fungi based on the number of conidia and dry weight of the conidia produced. Metarhizium sp. T4.B23 produced the highest number of conidia, 1.12 x 1011 conidia/100 g substrate and yielded 180.9 ± 0.623 g dry conidia/kg of rice; followed by Metarhizium sp. B2.2 grown on sorghum that resulted in 1.11 x 1010 conidia/100 g substrates and 8 ± 0.570 g /kg sorghum; and B. bassiana produced 8.3 x 109 conidia/100 g substrate and 31.24 ± 0.407 gr/ kg sorghum. Metarhizium sp. B2.2 showed the highest toxicity to termites with 100% mortality was observed within the second day of testing. Therefore the conidia of Metahizium sp. B2.2 is potential to be developed as a biopesticide using rice or sorghum substrate as a carrier.

CYTOTOXICITY AND ANTIPROLIFERATION OF PHYCOCYANIN FROM SPIRULINA PLATENSIS EXTRACT ON WIDR COLON CANCER CELL LINE Putri, Ajeng Kurniasari; Dimarti, Safira Chairani; Yuniati, Renni; Susilaningsih, Neni
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22881

Phycocyanin from Spirulina platensis extract has anticancer activity against various types of cancer cell cultures. However study about its effect on colon cancer cell lines, especially the WiDr, has not been reported before. This study aimed to reveal the anticancer activity of phycocyanin from Spirulina platensis extract on WiDr cells. The research was an in vitro experimental study, with the investigation on cytotoxicity also antiproliferation as the anticancer parameters. Both cytotoxicity and antiproliferation test was conducted through MTT assay to observe the visualization and inhibition of proliferation of different concentrations of phycocyanin in several incubation times on the WiDr colon cancer cell line. The obtained data were then processed statistically with the Two Way ANOVA test at a significance value of p <0.05 and followed with the Post Hoc test since there were significant differences. Based on the results, it could be postulated that phycocyanin extracted from freshwater Spirulina platensis was classified as non-toxic (IC50 of 855 µg/ml). Consequently, it is less potential to be used as the treatment for colon cancer. However, phycocyanin could inhibit the proliferation of the WiDr cell for approximately 47.4%, specifically at the concentration of 1710 µg/ml for 72 hours. It could be concluded that freshwater phycocyanin is less effective as an anticancer substance. The benefit of this study is to provide the new scientific evidence of the contrary results of freshwater phycocyanin activity from Spirulina platensis as an anticancer agent of colon cancer.

ISOLATION AND IDENTIFICATION OF ACTINOMYCETES ASSOCIATED WITH MOSS ON THE SURFACE OF THE BOROBUDUR TEMPLE STONE Putri, Ade Lia; Purbani, Debora Christin; Kanti, Atit; Kusmiati, Mia; Habibi, Moh
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.20334

Mosses growing on the surface of the Borobudur Temple will affect the aesthetic value of the temple. Interaction between moss and actinomycetes may trigger the growth of moss that can cause an increase in biodeterioration of stone. The purpose of this study was to isolate and identify the actinomycetes associated with moss on the surface of decayed stone of Borobudur Temple as well to assess their ability for phosphate solubilizing. Actinomycetes were isolated using serial dilution method and were identified based on 16S rRNA gene sequences. A total of 37 actinomycetes were isolated from three sampling sites. The isolates found belong to five genera (Gordonia, Microbacterium, Micromonospora, Nocardia, and Streptomyces) and distributed among four families (Microbacteriaceae, Micromonosporaceae, Nocardiaceae, and Streptomycetaceae). Isolates of actinomycetes composed of 19 Streptomyces Group and 18 Rare Actinomycetes Group. Nineteen isolates (51.35%) were identified as genus Streptomyces. Seventeen isolates (45.94%) showed abilities to release soluble phosphate and most of the isolates belong to the genus Streptomyces. The isolates have been collected will be deposited to Indonesian Culture Collection (InaCC) to enrich the collection of actinomycetes from ancient stone in Indonesia and will be used as a source of reference material research, taxonomic, or as source for further study.

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Location Kota semarang, Jawa tengah INDONESIA

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Contact Name
Dr. Ir. Dyah Rini Indriyanti

Contact Email
biosaintifika@mail.unnes.ac.id

Phone
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Journal Mail Official
biosaintifika@mail.unnes.ac.id

Editorial Address
-

Location
Kota semarang,

Jawa tengah

INDONESIA