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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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Indigenous Trichoderma harzianum as Biocontrol toward Blight Late Disease and Biomodulator in Potato Plant Productivity Purwantisari, Susiana; Sitepu, Harum; Rukmi, Isworo; Lunggani, Arina Tri; Budihardjo, Kadarwati
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.26706

Abstract

Indigenous Trichoderma has widely used in late-blight epidemic areas in Magelang, Indonesia as biocontrol and biomodulator. But it application still lacks of scientific proof, even though it is potently to be developed. Therefore, this study aims to identify and evaluate indigenous Trichoderma application in eradicating late blight and increase productivity. This research was an experimental posttest-only using six experimental plots, with P0 plot with no treatment. The solution was made by dissolve indigenous Trichoderma solid-starter into water (m/v), then used for treatment. The treatment plots including: two groups of sprayed-only plots with doses of 100 g/ 10 L of solution for P1 and 150 g/ 10 L of solution for P2; and two group treated with sprayed-poured method using doses of 100 g/ 10 L of solution for P3 and 150 g/ 10 L of solution for P4. The positive control group was P5 group, treated using chemical pesticides and tuber booster. The microscopic identification revealed that the local bioagent was Trichoderma harzianum species. Application of T. harzianum in P4 was significantly increased the productivity. However, it was not effective in increasing growth, but able to reduce the intensity of late blight disease. The research revealed that native Trichoderma is able to be used as anti-infectious agent and potentially improve the quality of potato plants. In the future, this research may worthwhile for farmers to develop and produce trustworthy and proven Trichoderma-based biocontrol and help them increase the potato production economically.
Plant Species Diversity and Degree of Homogeneity after the 2010 Eruption of Mount Merapi, Indonesia Afrianto, Whisnu Febry; Hikmat, Agus; Widyatmoko, Didik
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23525

Abstract

The eruption in 2010 of Merapi Mount changed the diversity of plant species. The objective of this research was to investigate the diversity of plant species on Mount Merapi National Park (MMNP). The sampling area was divided into three different levels of damage (heavy, medium, and mirror). The research was conducted by using line-transect and quadrat method.  We were conducted using line-transect and quadrat method. The research showed that Acacia decurrens has the highest Important Value Index (IVI) at the heavy damage area of Cangkringan Resort (CR) and Kemalang Resort (KR) as well as in mirror damage area of Selo Resort (SR). The most important species at medium damage area of Dukun Resort (DR) were Albizia lopantha and Pinus merkusii. The pioneer species at Non-Cover Area (NCA) among others were Trema cannabina, Pityrogramma calomelanos, Imperata cylindrical, Buddleja asiatica, Anaphalis javanica. Eupatorium riparium were the most important species of herb in all four locations based on SIMPER, but it was not dominant in NCA. The homogeneity of plant in Cangkringan Resort and Selo Resort (C < D < E) were normally distributed, whereas NCA, DR, and KR (E ≥ D) were homogeneous. Analysis of non-metric multidimensional scaling and value of ANOSIM (RANOSIM =0.69; p = 0.0001) demonstrated that all site locations have significance differences. This research was not only focusing on survey of vegetation diversity, but also frequency class distribution. Through this study of frequency of species provides a database for conservation biodiversity and restoration program.
Characteristics of DNA Barcodes from Three Thrixspermum Orchids Based on ITS2 Regions Rohimah, Siti; Ratnasari, Tri; Su’udi, Mukhamad
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23737

Abstract

Thrixspermum (T.) is one of the genus in Orchidaceae that has small flowers. Among species in this genus has a high homology and also has many synonyms. Identification using morphological characters can be constrained since Thrixspermum flowering time occurs in a very short period. This study aimed to conduct molecular-based identification of Thrixspermum orchids using DNA barcoding. This method applied molecular-based species identification technique using DNA sequences from genomic fragments that are considered fast, accurate, and consistent. The molecular markers used were Internal Transcribed Spacer 2 (ITS2), while the samples used were T. centipeda, T. lucidum, and T. angustifolium. BLAST results show that T. centipeda has a close relationship with T. centipeda from Malaysia (KX679332) with 99.79% percent identity, T. lucidum has a high homology with T. linusii (KX679333; 97.30%), while T. angustifolium has a high homology with T. triangulare (KX679348; 99.38%). There is a unique sequence that only T. lucidum and T. angustifolium have that distinguishes the two from other species. ITS2 can be recommended as a molecular marker for determining the Thrixspermum orchid barcode. The benefit obtained from this research is the DNA barcode sequences (ITS2) of Thrixspermum orchids would be very useful to enrich the plant barcodes database for further molecular taxonomy and biodiversity of orchid. 
Bifidobacterium longum, a Predominant Bifidobacterium in Early-life Infant Potentially Used as Probiotic Kusharyati, Dyah Fitri; Rovik, Anwar; Ryandini, Dini; Pramono, Hendro
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25895

Abstract

In early life, Bifidobacteria are reported as dominant bacteria in the human digestive tract. Bifidobacterium is potential as a probiotic. The probiotic property of Bifidobacterium is strain-specific. This study aimed to identify the Bifidobacterium (isolated from less than one-month-old healthy infant stool that potentially used as probiotic) based on the 16S rRNA gene and determining their similarities among Bifidobacteria. The probiotic-potentially Bifidobacterium was re-characterized by performing a Gram’s staining and catalase test. The DNA extraction process was followed by the 16S rRNA amplification using 27F-1492R primers. Sequence similarity was checked by using the BLAST program in the GenBank. The phylogenetic tree was constructed by using a neighbor-joining (NJ) method within the MEGA version 7.0 package. The 16S rRNA gene was presented at 1,500 bp length. Bifidobacterium strains have a 91.14-94.26 % sequence similarity to B. longum subsp. longum strain CCUG30698 which is considered as insufficient for species and genus identifications. However, those isolates could be assigned in a phylogenetic position. This present study suggested the B. longum as the dominant strain of Bifidobacterium in the gut of early-life infants which has potential as a probiotic and is considered as an ideal probiotic for human consumption. This study is useful as basic information for other related research, as well as its application in industrial or community service fields.
β-Glucans Production of Saccharomyces cerevisiae by Using Tofu Waste as Animal Feed Supplement Sulistya Dewi, Endah Rita; Nugroho, Ary Susatyo; Nurwahyunani, Atip; Ulfah, Maria
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.26754

Abstract

Tofu industrial waste has the potential to become contaminant when it is not maximum processed, so it needs appropriate treatment to minimize the occurrence of pollution. β-Glucans can be produced from the yeast cell wall of Saccharomyces cerevisiae.As a feed supplement, β-Glucans have been used for an immunostimulant because it can increase the immune system. This study aimed to establish a suitable technology to produce β-Glucans fromSaccharomyces cerevisiae by utilize tofu waste as feed supplement. The Completely Randomized Design (CRD) model was used in the experimental method. This study used an experimental method with a Completely Randomized Design (CRD) model.The treatment given wereP1: 1kg of tofu waste + 1kg of bran +0.2 kg fish meal + 0.2 lt molasses + 5.5 gSaccharomyces cerevisiae; P2: 1kg of tofu waste + 1kg of bran +0.2 kg fish meal + 0.2 lt molasses + 6.5 g Saccharomyces cerevisiae; P3: 1kg of tofu waste + 1kg bran + 0.2 kg fish meal +0.2 lt molasses + 7.5 g Saccharomyces cerevisiae. Each treatment was repeated 3 times, and fermented for five days.The results found that P1 by Saccharomyces cerevisiae 5.5 g was the best formulation to produced crude β-Glucans by weight of 25.9 g/kg of waste. Production of β-Glucans as an animal feed supplement is a waste-based feed technology development which is expected to increase livestock production both in quality and quantity.
Selection of Soybean (Glycine max) Germplasm Against Biotrophic Fungi Disease Based on Anatomical Resistance Samiyarsih, Siti; Pratiwi, Ade Yuanita Putri; Muljowati, Juni Safitri; Fitrianto, Nur
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25109

Abstract

The obstacle to increasing the soybean production is an infection of rust disease caused by the biotrophic fungus, Phakopsora pachyrhizi. The research objectives were to determine the anatomical resistance and the level of resistance of soybean cultivars against rust disease. The embedding method for observed leaf structural anatomy. The disease severity based on the method of International Working Group on the Soybean Rust (IWGSR) rating system.  The experiment was arranged as a Completely Randomized Design (CRD) with Factorial Pattern and five times repetition. The first factor was soybean cultivars, namely Gepak Kuning, Slamet, Tanggamus, and Wilis. The second factor was P. pachyrhizi inoculation with 0 uredospores/mL (uninoculated) and 104 uredospores/mL (inoculated). The results showed that the soybean cultivars that have thicker cuticle and epidermis, high trichomes and low stomatal density, and low of stomatal conductance have better anatomical resistance to leaf rust disease. Wilis and Slamet cultivars are resistant cultivars, indicated by disease intensity of 20% and 24.6%, respectively. While the Tanggamus is moderately resistant cultivar and Gepak Kuning is a susceptible cultivar, indicated by disease intensity of 56.5% and 85.3%, respectively. The novelty of selection soybean germplasm against biotrophic fungal disease are important and effectiveness in order to increase the crop productivity. These three soybean cultivars potentially serve as genetic sources to develop high yielding soybean cultivars and resistant to rust disease.
Frontmatter (Cover and Table of Contents) biosaintifika, biosaintifika
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.28867

Abstract

Ethnobotanical Study of Early Childhood Medicinal Plants Used by the Local People in South Bangka Regency, Indonesia Henri, Henri; Nababan, Vitryany; Hakim, Luchman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.22221

Abstract

South Bangka Regency has a long history of using medicinal plants as part of its medical system. However, the potential associated with this aspect of traditional medicine remains understudied and poorly explored. This study aimed to describe the types of medicinal plants and analyze the use of medicinal plants to treat early childhood disease by the local community of South Bangka Regency. The research method used was open interview with local people using the questionnaires. The data was analyzed using Use Value (UV), Fidelity Level (FL), and Relative Frequency of Citation (RFC). The analysis identified 55 plant species from 35 families. The most common families were Euphorbiaceae (8.92%), Fabaceae (7.14%), Poaceae (7.14%), Zingiberaceae (5.36%), and Lamiaceae (5.36%). The most widely used plant was shallot plants (Allium cepa L.) with values of UV, FL, and RFC were 0.67, 66.67, and 0.015, respectively. This study revealed the richness of ethnomedicinal knowledge in the South Bangka Regency. Finally, it is expected that this ethnobotany study can provide a database for further scientific research. The community’s knowledge as a legacy will not be repeated if it is not inherited. Besides, this biodiversity is very important as a socio-economic and ecological asset in South Bangka Regency which must be protected by all means from over exploitation.
Production of Sugar From Sweet Sorghum Stems with Hydrolysis Method Using Trichoderma viride Ndapamuri, Melycorianda Hubi; Herawati, Maria Marina; Meitiniarti, V Irene
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.25954

Abstract

Sorghum stem bagasse waste is one of the materials with high cellulose content. It can be utilized in glucose production through enzymatic hydrolysis of cellulose by Trichoderma viride. This study aims to determine sorghum stem bagasse’s potential in producing glucose, assessing the time and concentration of sorghum stem bagasse in the hydrolysis process to produce glucose optimally and following SNI. Hydrolysis was carried out using a concentration of 5%, 8%, and 11% sorghum stem bagasse for 10, 15, and 20 days. The results showed that sorghum stem bagasse waste could produce glucose with an average glucose yield of 10.09% to 24.40 %. There is a tendency that increasing substrate concentration and hydrolysis time will increase the yield of liquid glucose. The treatment of 5% concentration of sorghum stem bagasse with a long hydrolysis time of 10 days can produce the highest liquid glucose, namely 24.40% with total dissolved solids of 7.40% Brix, the ash content of 0.26%, but 47.54% water content has not met SNI standards. 
Antioxidant Activity of Jojoba (Simmondsia chinensis) Seed Residue Extract Siahaan, Arnold Patogi; Rohaeti, Eti; Muddathir, Ali Mahmoud; Batubara, Irmanida
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23323

Abstract

Jojoba (Simmondsia chinensis (Link)) is a shrub plant that widely used as cosmetic ingredients especially jojoba oil. When making jojoba oil, the residue still reminds and become waste. This study aimed to determine the antioxidant activity of jojoba seed residue (JSR). Jojoba seed residue was collected from Sudan. The JSR extracted by maceration with n-hexane, ethyl acetate, and 70% ethanol solvent. The antioxidant activity was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. The result showed that 70% ethanol extract of JSR had the highest antioxidant activity with 50% Inhibition Concentration (IC50) value of 219.42 mg/L. The 70% ethanol extract was fractionated by using silica gel column chromatography with gradient elution produced 10 fractions. All fractions showed antioxidant activity (IC50106-447 mg/L). Fraction 3 had the highest antioxidant activity with IC50 value of 106.26 mg/L. Therefore, JSR has a great potential for use as a raw material in cosmetic and pharmaceutical products industry, or as a source of bioactive compounds.

Page 45 of 107 | Total Record : 1068

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