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Bioma : Berkala Ilmiah Biologi

bioma
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Published by Universitas Diponegoro

ISSN : - EISSN : 25982370 DOI : -

Core Subject : Education, Agriculture,

Agriculture, Biological Sciences & Forestry Education

BIOMA (ISSN 1410-8801) adalah Majalah Ilmiah Biologi yang bertujuan mewadahi semua informasi hasil penelitian, telaah pustaka, makalah teknis, dan kajian buku, dari berbagai cabang ilmu Biologi. Diharapkan penerbitan ini dapat berkontribusi dalam penyampaian informasi ilmiah yang merupakan mata rantai proses kegiatan ilmiah. Terbit sejak tahun 1999.

Arjuna Subject : -

Articles 318 Documents

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Prediksi Resistensi Udang Vaname (Litopenaus vannamei) terhadap Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) dari Tambak Intensif dan Semi Intensif Jepara Menggunakan Marka RAPD Mulyadi, Muhammad; Handayani, Christina Ratna; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto
Bioma : Berkala Ilmiah Biologi Vol. 15, No.2, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Infectious Hypodermal and Hematopoietic Necrosis Infectious Virus (IHHNV) is the most important DNA virus which can lead to Runt Deformity Syndrome (RDS) in vaname shrimp. The RAPD technique can be used to determine the resistance of a species to a disease. This research aimed to screen and identify RAPD markers which could distinguish the resistance of vaname shrimp to IHHNV reared at intensive and semi-intensive pond. The DNA template was amplified by PCR using 5 primers : OPA 06, OPA 08, OPA 19, OPD-02 and OPZ-15. The results showed that only the primer OPA-19 and OPZ-15 were able to produce 100% polymorphic bands with sizes from 400-1700 bp as well as showing the resistance IHHNV in vaname shrimp. Based on these results, vaname shrimp which reared at the intensive pond were more resistant to IHHNV compared with the semi-intensive pond. Â Key words: RAPD, shrimp vaname, IHHNV, intensive & semi-intensive pond Â

Optimasi Produksi Inulinase oleh Khamir Pichia manshurica DUCC Y-015 pada Tepung Umbi Dahlia (Dahlia variabilis Willd.) dengan Variasi Konsentrasi K2HPO4 dan Waktu Inkubasi Amedia, Inggrit; Wijanarka, Wijanarka; Purwantisari, Susiana
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Sugar national supply more and more decreases and can not meet the market needs. The research has been done to find alternative natural sweeteners including inulin from dahlia tubers (D. variabilis Willd). Dahlia tuber can produce 95% of yield of fructose syrup in an enzimatic reaction by inulinase (E.C.3.2.1.7). Inulinase is inductive enzyme that can be produce by P. manshurica. The production of fructose needs to be optimized to get optimum product. The optimization can be done by modifying the nutrient content in the medium such as K2HPO4 and variation of incubation time. The purpose of this study is to determine the concentration of K2HPO4 and optimum incubation time for P. manshurica. This research was conducted in Microbiology Laboratory, Biology Department, Faculty of Science and Mathematics Undip. The examined variable is the growth of yeast cell, inulinase activity, invertase, and the I/S ratio. This research was conducted experimentally using Randomized Complete Block Design (RCBD) factorial pattern with 2 factors, the first factor was the concentration of K2HPO4 (P), with concentrationÂ level (g/L) of 0.5 (P1), 1.0 (P2), and 1.5 (P3). The second factor was incubation time (W) with 12 hours (W12), 18 hours (W18), and 24 hours (W24). Every treatment was repeated three times. The collected data were analyzed using ANOVA. If there was a treatment effect, it will be continued with Duncan test on 5% significance level. The result of analysis show that the highest cell growth and the maximum production of inulinase enzyme was in P3W24 occurs in P3W24 (K2HPO4 1.5 g/L and 24 hours incubation time) treatment at 0.428 IU, but efficient in P1W12 treatment as much as 0.365 IU.Â Keywords: Dahlia variabilis Willd., Inulinase, K2HPO4, Pichia manshurica DUCC Y-015, incubation time

Optimasi Produksi Inulinase isolat P 12 pada Tepung Umbi Dahlia ( Dahlia variabilis Wild ) dengan Variasi Konsentrasi Nitrogen Organik dan Waktu Inkubasi Lunggani, Arina Tri; Wijanarka, Wijanarka; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 12, No. 1, Tahun 2010
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Efforts to address health problems mainly related to the digestive tract is by consuming one prebiotic, eg fruktooligosakarida (FOS). FOS is a prebiotic that one species can be produced from the hydrolysis of inulin using inulinase enzyme. Isolate P12 is an isolate that has been proven to have high inulinase activity on standard medium inulin production. Inulinase production increase can be done by adding a source of organic nitrogen in the form of yeast extract in medium. The results indicate that the best on the concentration of nitrogen concentration P2 (Yeast extract 0.25%) with the activity of 0.7983 IU, while the best 12-hour incubation time with the activity of 0.7899 IU. Likewise for the best interaction P2 T2 treatment with inulinase activity of 0.9025 IU.

Identifikasi Talas-Talasan Edible (Araceae) Di Semarang, Jawa Tengah Sinaga, Khalisa Aini; Murningsih, M; Jumari, J
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Talas-talasan (Family Araceae) is ) is a plant known to the Indonesian people. Araceae has been cultivated and used by Indonesian people. Tuber of Family Araceae have high carbohydrate, carbohydrate composed of amylum (amylose and amylopectin). food quality is determined by ratio of amylose and amylopectin. Information about Araceae in Semarang are still limit. Indonesian people didnâ€™t know potential of the family araceae in field of food. The research objective to determine types ofedible plants from the family Araceae.The research was conducted from April to June 2016. Sampling was conducted at Jabungan, Mluweh and Susukan village, Semarang. Methods of data collection is ekploration. Identification refers to the genera of Araceae (Mayo, 1997) and characterization refers Descriptor Taro (IPGRI, 1999). Based on the results is 13 species of plants from the family Araceae. Differences among species Family Araceae character is the color and shape of the tubers, the tip of leaf blade, the edge of leaf blade, the top and bottom surface of leaf blade, leaf blade holder, and the shape of leaf blade.Â Kata kunci :Identification, Morphology Character, Araceae

Uji Ekstrak Bawang Bombay sebagai Anti Bakteri Gram Positif Staphylococcus aureus dengan Metode Difusi Cakram Wuryanti, Wuryanti; Mulyani, Nies Suci; Asy'ari, Mukhammad; Sarjono, Purbowatiningrum Ria
Bioma : Berkala Ilmiah Biologi Vol. 12, No. 2, Tahun 2010
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Microbe is a microscopic living organism which is closely linked to human life. One of pathogenic microorganisms such as Staphylococcus aureu,. may cause diseases. Sulfure of several plants are bioactive as antimicrobial. The properties of sulfure in Allium cepa L leads to an assumption that it has antimicrobial properties. Therefore, this study involved the antimicrobial test. The determination steps of antimicrobial Allium cepa L extract consisted of the Allium cepa L extract preparation, microbial regeneration and antimicrobial test. The antibacterial determination were performed by paper disc method. Based on the research data, determination of antimicrobial Allium cepa L extract resulted in inhibition zone area showed that the extract owned antimicrobial property.

Karakterisasi Uwi-Uwian (Dioscorea spp) Dari Banjarnegara Berdasarkan Penanda Morfologi Pertiwa, Susy Ika; Jumari, J; Wiryani, Erry
Bioma : Berkala Ilmiah Biologi Vol. 20, No 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Â Â Â The diversity of Dioscorea spp. both inter-species and intra-species are spreaded in several areas in Central Java, including Banjarnegara.Â Dioscorea is aÂ plant that has the potentials to be developed and cultivated. The purposes of this reasearch are to identify the species, characterize the morphological characters (stem, leaf, and tuber) of eight varians Dioscorea spp from Banjarnegara. The sample used in this reasearch are the eight varians of Dioscorea spp from Banjarnegara.Â The Identification was done by using a identification keys. Characterization of morphological characters was done by using the guidebook of Descriptor for Yam. The results showed that eight cultivars of Dioscorea spp are grouped into two types: Dioscorea alata and Dioscorea esculenta. Dioscorea alata has a stem which rotate the to the right, rectangular shaped of stem, itâ€™s has wing and doesnâ€™t have a spin, elongated heart-shaped leaves, oval-oblong and round shaped tuber. Dioscorea esculenta has a stem which rotate to the left, rounded shaped of stem, it has not wing but spiked, dilated heart-shaped leaf, and oval shape tuber. Â Keywords: Dioscorea spp, identifikasi, characteristic, morphology,

Optimasi Isolasi DNA Cabai (Capsicum annuum L.) Berdasar Perbedaan Kualitas dan Kuantitas Daun serta Teknik Penggerusan Ferniah, Rejeki Siti; Pujiyanto, Sri
Bioma : Berkala Ilmiah Biologi Vol. 15, No.1, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Complete genome of chili has not been reported.The first step to study the genome is DNA isolation, so it is necessary to optimize the protocol to get an optimum DNA. This research aimed to optimize chili DNA isolation by variate the quantity and quality of chili leaves as row material and variate the grinding technique. DNA isolation was done using commercial kit without liquid nitrogen, and analyzed by agarose gel electrophoresis. The results showed that frozen chili leaf yields more DNA than fresh leaf, 0,1 g of leaf got optimum DNA, and grinding in mortar was better than in microtube. Â Key words: DNA,Â isolation, Capsicum annuum

Identifikasi Tumbuhan Lumut di Kawasan Wisata Gunung Galunggung Kabupaten Tasikmalaya Jawa Barat Putra, Rinaldi Rizal; Hernawati, Diana; Fitriani, Rita
Bioma : Berkala Ilmiah Biologi Vol. 21, No 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Galunggung mountain tourism spot is one of the natural tourism objects in Tasikmalaya regency possessing high biodiversity of living things, notably moss. The well-preserved environmental conditions in the area of Galunggung mountain enable the moss to grow luxuriantly and has miscellaneous varieties. This study aimed at identifying the moss living in around Galunggung mountain, Tasikmalaya. It was conducted on November 2017 through descriptive exploratory method with survey technique. The data were analysed descriptively by collecting specimens of the moss discovered in each observing station. The observing locations were situated in three different locations (stations) based on hierarchical height levels. Based on the identification, the moss proliferating in Galunggung mountain were classified into 20 species. The moss species dominantly identified in the entire observing stations were Bryum billardierii, Acroporium sp., Leucobryum sp., Marchantia polymorpha, and Calymperes sp. Â Â

Allelopathic Effect of Cyperus rotundus L. on Seed Germination and Initial Growth of Glycine max L. cv. Grobogan Darmanti, Sri; Santosa, Santosa; Dewi, Kumala; Nugroho, L Hartanto
Bioma : Berkala Ilmiah Biologi Vol. 17, No.2, Tahun 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Allelopathy is a phenomenon of direct or indirect, beneficial or adverse effects of a plant on its own or another plant through the release of chemicals into the environment. This experiment was carried out to determine the allelopathic effect of Cyperus rotundus L. (purple nutsedge) tuber aqueous extract on seed germination and initial growth of Glycine max L. (soybean) seedlings. The experiment was performed in completely randomized (CRD) design with five replications, using a range of concentrations of aqueous tuber extracts of nutsedge, i.e.: 0%, 5%, 10%, 15%, 20% and 25%. The petri dish experiment showed that with increase of the tuber extract concentration, a significant increase was noted in mean germination time (MGT), significant decreases in germination index (GI), growth tolerance index (GTI), wet weight seedling, dry weight seedling andÂ length of soybean seedling.Â Â

Uji Potensi Bakteri Endofit Kitinolitik Tanaman Padi (Oryza sativa L.) Sebagai Penghasil Hormon IAA (Indole Acetic Acid) Hanafi, Ahmad; Purwantisari, Susiana; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

IAA (Indole Acetic Acid) is a hormone in plants that was role in the cleavage of roots, inhibits the growth of side shoots, stimulate cell division and the formation of xylem and phloem tissue. This study aimed to test the potential of endophytic bacteria chitinolytic rice crop as hormone-producing IAA. This study uses 9 isolates of endophytic bacteria chitinolytic rice plants in isolation during practical work. The experiment consisted of 15 treatments and 3 replications. This study uses a randomized block design. The treatments tryptophan concentration combined with a variation pH, the endophytic bacteria grown on media chitinolytic tryptophan concentration of 0 mg/L, 102 mg/L, 204 mg/L, 306 mg/L and 408 mg/L are combined with pH 5, 7 and 9. the treatment was observed for 48 hours and observation once every 3 hours. The measured variable is the result of the production of IAA hormone with the treatment combination of tryptophan with pH. IAA hormone outcome data were analyzed using Analysis of Variance Univariates at level of 95%. IAA hormone qualitative test results showed positive results in bacterial isolates KA12, KA11 and KB24. IAA hormone quantitative results of bacterial isolates producing IAA hormone KA12 high of 2,03 mg/L in the combination treatment of tryptophan 408 mg/L at pH 7 at 24 hours incubation. KA12 bacterial isolates are endophytic bacteria chitinolytic potential to produce hormones IAA, yet the results of data analysis showed that each treatment combination with pH tryptophan to IAA production were not significantly different.Â Keywords: hormone IAA, chitinolytic endophytic bacteria, tryptophan, pH

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