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Klinikal Sains (Jurnal Analis Kesehatan)
Published by Universitas Abdurrab
ISSN : 23384921     EISSN : 26141515     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology
Jurnal klinikal sains program studi analis kesehatan adalah jurnal ilmiah yang membahas tentang kompetensi analis kesehatan yang meliputi kompetensi Mikrobiologi, Hematologi, Parasitologi, Imunoserologi, Toxikologi, dan Kimia Klinik. Jurnal klinikal sains akan terbit setiap enam bulan, dalam satu periode setiap tahun ajaran akan terbit dua kali yakni pada bulan Juni dan Desember.
Arjuna Subject : -
Articles 245 Documents
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PEMANFAATAN ENZIM PAPAIN DARI PEPAYA LOKAL SEBAGAI PROTEASE UNTUK ISOLASI DNA GENOME BAKTERI DALAM MENUNJANG PEMERIKSAAN POLYMERASE CHAIN REACTION (PCR) Fini Ainun Qolbi Wasdili; Dwi Davidson Rihibiha; Ellsie Viendra Permana
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 1 (2024): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i1.4298

Abstract

Polymerase chain Reaction (PCR) is affected by many factors, one of which is DNA isolation. Proteinase K is a common protease used in DNA isolation. However, proteinase K is relatively expensive and limited. To date, many studies have been conducted to explore alternative protease from nature. Papain is an enzyme derived from papaya, which has been frequently observed, makes it promising as protease in DNA isolation. This study aims to evaluate potency of papain as cheaper alternative in DNA isolation from Salmonella thypi. This study consisted of four steps: papain extraction, characterization of papain, DNA isolation, and fliC detection with PCR. Papain was acquired with tapping method which resulted in approximately 1700 μl bp. Molecular weight of papain was 23,4 kDa, and its total concentration was 903 mg/mL. Whole genom of Salmonella thypi was successfully isolated using papain. The DNA concentration was also comparable to control. Nevertheless, the purity was low which is not recommended to be used in PCR.
HUBUNGAN KEJADIAN HIPERTENSI DENGAN PROTEIN URINE PADA IBU HAMIL TRIMESTER III DI RSUD dr. SOEDIRMAN KEBUMEN: THE CORRELATION BETWEEN THE INCIDENCE OF HYPERTENSION AND URINE PROTEIN IN THIRD TRIMESTER PREGNANT WOMEN AT RSUD dr. SOEDIRMAN KEBUMEN Dwiar Apriyanti; Retno Sulistiyowati; Tantri Analisawati Sudarsono; Minto Rahaju
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 1 (2024): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i1.4508

Abstract

Abstract The prevalence of hypertension during pregnancy is 2.6 - 7.3% of all pregnancy incidents which can have risks for pregnant women, which is usually called the Maternal Mortality Rate (AKI). Hypertension during pregnancy is one of the causes of maternal death which can be detected early through a urine protein test. Urine protein is an important sign of preeclampsia which is caused by the kidney tubules not having optimal capacity to absorb protein. Based on research by Wulandari et al (2022) which states that there is a significant relationship between urine protein and blood pressure levels in TM III pregnant women at the Madukara I Banjarnegara Community Health Center with p=0.003. The aim of the study was to determine the relationship between the incidence of hypertension and urine protein in pregnant women in TM III at RSUD dr. Soedirman Kebumen. The research method used Observational Analytical through a Cross-Sectional design with a sample of 40 people, carried out in May - July 2023 at the Laboratory of RSUD dr. Soedirman Kebumen. Based on this research, a value of p=0.052 (p>0.05) was obtained, so it can be concluded that there is no significant relationship between the incidence of hypertension and urine protein in TM III pregnant women at Dr. Soedirman Hospital, Kebumen. Keywords: Hypertension, Third Trimester pregnant women, urine protein.
GAMBARAN JUMLAH KOLONI Candida albicans TERHADAP KADAR GULA DARAH SEWAKTU PADA WANITA PENDERITA DIABETES MELLITUS Anik Puspita Ningrum; Muhammad Taufiq Qurrohman
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 1 (2024): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i1.4632

Abstract

Diabetes mellitus terjadi dikarrenakan adanya gangguan pada metabolisme karbohidrat yang tidak stabil sehingga peningkatan kadar gula darah. Kenaikan kadar gula darah ini menyebabkan pertumbuhan jamur Candida albicans. Penelitian ini bertujuan untuk menilai jumlah koloni Candida albicans dalam urin pasien diabetes mellitus terkait dengan kadar glukosa darah sewaktu (GDS). Metode yang digunakan pada penelitian ini adalah metode deskriptif, yaitu dengan menggunakan metode pemeriksaan kadar glukosa darah sewaktu (GDS) menggunakan point-of-care testing (POCT), dan pemeriksaan kadar glukosa urine menggunakan metode celup. Penelitian dilakukan di laboratorium parasitologi Sekolah Tinggi Ilmu Kesehatan Nasional Surakarta, dengan teknik sampling purposive sampling terhadap 19 responden. Hasil penelitian menunjukkan bahwa nilai GDS berkisar antara 101 mg/dL hingga 313 mg/dL, dengan mayoritas responden memiliki GDS di atas batas normal. Pada wanita penderita diabetes mellitus, mayoritas hasil pemeriksaan glukosuria negatif, namun satu responden menunjukkan hasil positif. Temuan penelitian menunjukkan adanya pertumbuhan jamur Candida albicans pada urine wanita penderita diabetes mellitus di Puskesmas Karanganyar. Dengan demikian, kesimpulan yang didapatkan dari penelitian ini adalah mayoritas respondendengani kadar GDS yang melebihi batas normal terdapat peningkatan jumlah koloni Candida albicans.
GAMBARAN KEJADIAN PENYAKIT MENULAR SEKSUAL DALAM PEMERIKSAAN TRIPLE ELIMINASI PADA IBU HAMIL DI SIDOARJO Galuh Destika Rahmadhanti; Farida Fitriana; Ivon Diah Wittiarika
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 1 (2024): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i1.4641

Abstract

Ibu hamil merupakan salah satu dari populasi yang berisiko tertular penyakit menular seksual seperti Human Immunodeficiency Virus (HIV), Sifilis, dan Hepatitis B. Risiko penularan dari ibu ke anak untuk penyakit HIV/AIDS adalah 20-45%, untuk Sifilis adalah 69-80%, dan untuk Hepatitis B adalah lebih dari 90%. Penelitian ini bertujuan untuk mengetahui gambaran kasus penyakit menular seksual pada ibu hamil di Sidoarjo. Jenis penelitian yang digunakan adalah deskriptif dengan pendekatan kuantitatif. Populasi penelitian ini adalah ibu hamil yang memeriksakan kehamilannya di seluruh Puskesmas Kabupaten Sidoarjo pada tahun 2023. Sampel Penelitian ini adalah data pemeriksaan triple eliminasi pada K1 ANC di seluruh Puskesmas Kabupaten Sidoarjo Tahun 2023 (populasi total). Hasil penelitian menunjukkan kelompok usia 20-35 tahun adalah yang terbanyak pada ibu hamil HIV (87,5%), sifilis (90,0%), dan Hepatitis B (79,0%) Ibu hamil yang tidak bekerja adalah yang terbanyak pada penderita HIV (82,5%), sifilis (90,0%), dan hepatitis B (67,4%). Berdasarkan jumlah paritas ibu nullipara adalah yang terbanyak pada ibu HIV (46,3%), ibu sifilis terbanyak pada kelompok primipara (60,0%), HBsAg terbanyak pada kelompok primipara (41,7%). Usia kehamilan terbanyak pada ibu hamil dengan HIV adalah ≥25 minggu (47,5%), ibu hamil dengan sifilis terbanyak pada ≤ 12 minggu dan ≥25 minggu (40%), ibu hamil dengan hepatitis B terbanyak pada usia kehamilan 13-24 minggu (33,8%). Kesimpulannya usia, pekerjaan, jumlah paritas, dan usia kehamilan adalah karakteristik pada ibu hamil yang melakukan pemeriksaan triple eliminasi di puskesmas di Sidoarjo.
PERBEDAAN HASIL PEMERIKSAAN TUBERCULOSIS PASIEN DM DAN NON DM MENGGUNAKAN SKALA IUATLD DI UPTD PUSKESMAS CITANGKIL KOTA CILEGON Dwi Oktaviyaningsih; Ardy Prian Nirwana
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 1 (2025): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikalsains.v13i1.4760

Abstract

Diabetes mellitus is a disease that is very dominant as a comorbid disease of pulmonary TB. People with DM have a weakened immune system so they are three times more likely to get active TB. TB patients who have been diagnosed with DM have a high risk of mortality during TB treatment and a risk of relapse after completing treatment. The aim of this study was to determine the difference in tuberculosis examination results in DM and non-DM patients using the IUATLD Scale. The research method was descriptive with a cross sectional approach with a sample size of 30 respondents divided into two, 15 respondents in the TB-DM group and 15 respondents in the TB Non-DM group. The sampling technique used was quota sampling. The data analysis techniques used are bivariate analysis. The results of univariate analysis showed that the majority of TB-DM sufferers were detected as positive for 3+ (46.7%) and the majority of non-DM TB sufferers were detected as positive for 1+ (53.3%). The results of bivariate analysis showed that statistically there was no difference in the results of tuberculosis examinations in TB-DM and non-DM TB patients using the IUATLD scale at the Citangkil Health Center, Cilegon City (p value: 0.301).
HUBUNGAN PERILAKU HIDUP BERSIH DAN SEHAT (PHBS) DENGAN INFEKSI SOIL TRANSMITTED HELMINTHS (STHs) PADA ANAK SD DI TIWOHO KECAMATAN WORI Indra Elisabet Lalangpuling; Mouren Irene Yusuf; Desinta Samala Majampoh; Kevin Giovani Pascoal; Michael V.L. Tumbol; Suwarja Suwarja; Rosdiana Naibey
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4916

Abstract

One of the factors that causes Soil Transmitted Helminths infection (intestinal nematodes that are transmitted through soil) is Clean and Healthy Living Behavior (PHBS). STHs usually breed in tropical areas with high temperatures and humidity. The aim of this research was to find out whether STHs worm infection which was transmitted through soil in elementary school children in Tiwoho Village, Wori District was related to clean and healthy living behavior (PHBS). This type of research is descriptive with a sampling method, namely purposive sampling of 65 stool samples that meet the inclusion criteria. Stool samples were examined microscopically using the native method, flotation method and Baerman Test method. The Square test was used in data analysis to assess whether there was a relationship between PHBS and STHs worm infection. The results of this study show that there is a relationship between Clean and Healthy Living Behavior (PHBS) and STHs worm infection, especially the habit of washing hands with soap before eating and the habit of biting nails with a p value <0.005.
UJI AKTIVITAS ANTIOKSIDAN EKSTRAK AIR TANAMAN SEMANGGI (Marsilea crenata C.Presl) METODE FRAP (Ferric Reducing Antioxidant Power) raisya adila adila; I.A.K. Pramushinta; A.S. Sinulingga
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4952

Abstract

The clover plant (Marsilea crenata C.Presl) is a plant that is widely used for food and has the potential to be developed as a herbal medicine. Clover plant extract using methanol, chloroform, and ethyl acetate was found to have weak antioxidant activity. The aim of this research was to test the antioxidant activity in air solvent extracts of clover plants using the FRAP (Ferric Reducing Antioxidant Power) method and analyze the effect of variations in extract concentration on the antioxidant activity test. The method in this research is clover plant extraction by maceration using an air solvent. Test antioxidant activity using the FRAP method using a UV-Vis spectrophotometer with a maximum wavelength of 596 nm. The extract yield of 42% showed that it positively contained flavonoids, saponins, polyphenols and a water content of 9.94% ± 0.1276. The results showed that clover plants had weak antioxidant activity (IC50 351.04 ppm) compared to vitamin C as a positive control which showed very strong antioxidant activity (IC50 22.60 ppm). From the results of this study, it can be said that increasing the extract concentration can increase the percentage of inhibition of free radical formation, but the increase is not linear, as indicated by the R2 value not approaching 0.99.
PERBANDINGAN PERTUMBUHAN Aspergillus niger PADA MEDIA POTATO DEXTROSE AGAR (PDA) DAN MEDIA ALTERNATIF DARI SINGKONG, UBI JALAR, DAN BEKATUL Indah Lestari; Akhmad Mubarok; Imam Agus Faizal
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4955

Abstract

Aspergillus niger is one of the fungi that can produce citric acid and is often used in some studies because of its resistance to very low acidic conditions. Potato Dextrose Agar medium is a common medium for culturing fungi in the laboratory. Alternative media are media that use materials from nature whose content is not yet known in detail. Rubber cassava has a carbohydrate content of 98.4674%. The carbohydrate content of white sweet potato is 28.78%. Rice bran has a carbohydrate content of 84.36%. The purpose of this study was to determine the comparison of the growth diameter of Aspergillus niger fungi on Potato Dextrose Agar (PDA) media and alternative media made from cassava, sweet potato, and rice bran. The research used a pure experimental method. Aspergillus niger isolates were observed microscopically, after which they were planted on PDA media and alternative media, incubated at 37°C for 72 hours, then the diameter of fungal growth was measured. The results showed that the alternative media that had the largest Aspergillus niger fungal growth diameter compared to PDA media as a control was alternative media from rice bran with the smallest diameter of 40 mm and the largest diameter of 80 mm.
PERBANDINGAN KADAR HEMOGLOBIN, GLUKOSA DARAH, DAN LEUKOSIT SEBELUM DAN SESUDAH HEMODIALISA PADA PASIEN GAGAL GINJAL KRONIS KOMORBID DAN NON KOMORBID DIABETES MELLITUS DI RSPC Risma Adzkia Putri; Ira Pangesti; Meka Faizal Farabi
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4956

Abstract

Chronic Kidney Disease (CKD) is an impairment of the glomerular organ function of the kidneys. The process of blood purification by collecting waste is called hemodialysis. The prevalence of CKD increased from 1990 to 2017 globally by 21.3% and the mortality rate increased by 41.5%. The purpose of this study was to compare hemoglobin, leukocyte, and blood glucose levels before and after hemodialysis in patients with and without comorbid Diabetes Mellitus (DM) at Pertamina Cilacap Hospital (RSPC). The method used was cross sectional using univariate and bivariate analysis with JASP program. The results showed that in CKD patients without DM, hemoglobin levels were significantly different (p<0.05) after hemodialysis. While leukocyte and glucose levels had no significant difference (p>0.05). In CKD patients with DM, there is a significant difference in hemoglobin levels (p<0.05) after hemodialysis. In addition, there is a significant difference in glucose levels (p<0.05) after hemodialysis. Meanwhile, there was no significant difference in leukocyte levels (p>0.05). Comparison of pre and post hemodialysis between CKD without DM and CKD with DM there is a significant difference (p<0.05) in hemoglobin and blood glucose levels but there is no significant difference (p>0.05) in leukocyte levels.
UJI AKTIVITAS ANTIOKSIDAN INFUSA SEMANGGI (Marsilea crenata C. Presl) MENGGUNAKAN METODE DPPH (1,1-Diphenyl-2-PicrylHydrazyl) Tiara Juliar Subakti; IAK Pramushinta; AS Sinulingga; SA Devi
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4966

Abstract

Changes in lifestyle and environment now increase the risk of exposure to free radicals that cause degenerative diseases. Compounds needed to neutralize free radicals are antioxidants. One source of antioxidants is clover (Marsilea crenata C. Presl), which has previously been studied and has shown no antioxidant activity in various extraction solvents, but the clover infusion method with water solvent has not been studied, so the purpose of this study was to determine the antioxidant activity of clover infusion (Marsilea crenata C. Presl). This research method uses the DPPH method, then the antioxidant activity test of clover infusion (10% b/v) with a concentration of 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm, and vitamin C as a positive control measured by a UV-Vis spectrophotometer. The absorbance value obtained at the maximum DPPH wavelength (516 nm) was used to calculate the linear regression equation of concentration and% inhibition. The results of the study obtained the IC50 value of clover infusion of 804.0 ppm and vitamin C as a positive control of 29.1 ppm. The conclusion of the study is that clover infusion shows no antioxidant activity (IC50 > 500 ppm), while vitamin C shows very strong antioxidant activity (IC50 > 50 ppm). Further research suggestions are related to the development of clover plant extraction methods and solvents (Marsilea crenata C. Presl) and antioxidant activity test methods.

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