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GC-MS Study of Bioactive Compound of Peperomia pellucida and Its Antibacterial Activity against Streptococcus mutans Kuswati, Kuswati; Rosalinda, Julitha Dwi; Waluyo, Joko
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.18877

Streptococcus mutans is the prior bacteria causing dental caries. Suruhan (Peperomia pellucida) may prevent dental caries due to its antibacterial bioactive content. This study aims to identify the bioactive profile of Suruhan ethanol extract and its antibacterial activity against Streptococcus mutans. Bioactive compounds were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). Antibacterial activity was tested through an inhibition test using the well-diffusion method, the Minimum Inhibitory Concentration (MIC) test, and the Minimum Bactericidal Concentration (MBC) test. The most detected compounds in Suruhan ethanol extract were n-eicosane, n-hexadecane, and glycerol. There are also antibacterial bioactives such as phenols, flavonoids, alkaloids, and terpenoids. Inhibition test of 500 mg/mL extract showed a clear zone of 8.25 mm diameter. The MIC and MBC values were 50 mg/mL and 100 mg/mL, respectively. Based on the results, Suruhan ethanol extract shows potential as an antibacterial, although its inhibitory effectiveness could be better.

In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Wiharyanti, Risma; Mercuriani, Ixora Sartika; Yulianti, Evy; Kuswandi, Paramita Cahyaningrum
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA

In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Wiharyanti, Risma; Mercuriani, Ixora Sartika; Yulianti, Evy; Kuswandi, Paramita Cahyaningrum
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA

Antioxidant Content and Vitamin C of Combination Tea of Rose Flower and Katuk Leaf based on Drying Temperature Variation Viscasari, Berliana; Ambarwati
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25685

Rose flowers and katuk leaves have not been maximally utilized. people only use rose flowers as ornamental plants and katuk leaves as vegetables. Though many bioactive compounds contained in both plants such as antioxidants and vitamin C. This study aims to examine the effect of drying temperature on the physical and chemical properties of tea combined with rose flowers and katuk leaves. the research method used a factorial complete randomized design (CRD) with a variation of drying temperatures of 50°C, 60°C, and 70°C for 5 hours, each repeated 3 times. the research parameters included chemical analysis, namely antioxidant levels (DPPH method), and vitamin C (iodimetry titration), as well as analysis of physical properties, namely pH value (pH meter), and evaluation of color, taste, and aroma. The results showed that drying temperature had a significant effect on antioxidant levels, vitamin C, pH value, color, and taste of tea, but had no effect on aroma. Drying temperature of 50°C produced the best quality with antioxidant content of 49.60%, vitamin C of 85.05 mg/100g, pH value of 6.56, light brown color, slightly bitter taste, and distinctive aroma of katuk leaves. In conclusion, a drying temperature of 50°C is recommended to produce rose flower and katuk leaf combination tea with the best physical and chemical quality.

Antioxidant Content and Vitamin C of Combination Tea of Rose Flower and Katuk Leaf based on Drying Temperature Variation Viscasari, Berliana; Ambarwati
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25685

Rose flowers and katuk leaves have not been maximally utilized. people only use rose flowers as ornamental plants and katuk leaves as vegetables. Though many bioactive compounds contained in both plants such as antioxidants and vitamin C. This study aims to examine the effect of drying temperature on the physical and chemical properties of tea combined with rose flowers and katuk leaves. the research method used a factorial complete randomized design (CRD) with a variation of drying temperatures of 50°C, 60°C, and 70°C for 5 hours, each repeated 3 times. the research parameters included chemical analysis, namely antioxidant levels (DPPH method), and vitamin C (iodimetry titration), as well as analysis of physical properties, namely pH value (pH meter), and evaluation of color, taste, and aroma. The results showed that drying temperature had a significant effect on antioxidant levels, vitamin C, pH value, color, and taste of tea, but had no effect on aroma. Drying temperature of 50°C produced the best quality with antioxidant content of 49.60%, vitamin C of 85.05 mg/100g, pH value of 6.56, light brown color, slightly bitter taste, and distinctive aroma of katuk leaves. In conclusion, a drying temperature of 50°C is recommended to produce rose flower and katuk leaf combination tea with the best physical and chemical quality.

Epidermal Structure and Phytochemical Screening of Palm Leaves (Family Arecaceae) Husnudin, Uni Baroroh; Ekasari, Munifilia; Erliana, Dina; Setiyaningrum, Dewi A; Firdaus, Widani
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25714

The epidermal structure and phytochemical composition of six palm species (Arecaceae) were studied to understand their anatomical characteristics and bioactive compound content. Leaf samples from phoenix palm (Phoenix roebelenii), red palm (Cyrtostachys renda), foxtail palm (Wodyetia bifurcata), fan palm (Livistona chinensis), christmas palm (Adonidia merrillii), and yellow palm (Chrysalldocarpus lutescens) were analyzed through microscopic observation and phytochemical screening. The results revealed variations in epidermal cell shape, cell wall structure, and stomatal distribution. Most species exhibited smooth-walled epidermal cells, except for red palm (C. renda), which had wavy cell walls. Stomata were predominantly tetracytic, with red palm (C. renda) and christmas palm (A. merrillii) displaying an amphistomatic distribution, while the other species were hypostomatic. Phytochemical analysis revealed the presence of flavonoids and saponins in all samples. Triterpenoids were detected only in phoenix palm (P. roebelenii), fan palm (L. chinensis), and yellow palm (C. lutescens). Alkaloids were found in phoenix palm (P. roebelenii) and red palm (C. renda), while steroids were present in red palm (C. renda), foxtail palm (W. bifurcata), and christmas palm (A. merrillii). Additionally, tannins were detected in all plant samples except for fan palm (L. chinensis). These findings suggest that palm leaves possess significant bioactive compounds that could be explored for pharmaceutical and industrial applications.

Epidermal Structure and Phytochemical Screening of Palm Leaves (Family Arecaceae) Husnudin, Uni Baroroh; Ekasari, Munifilia; Erliana, Dina; Setiyaningrum, Dewi A; Firdaus, Widani
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25714

The epidermal structure and phytochemical composition of six palm species (Arecaceae) were studied to understand their anatomical characteristics and bioactive compound content. Leaf samples from phoenix palm (Phoenix roebelenii), red palm (Cyrtostachys renda), foxtail palm (Wodyetia bifurcata), fan palm (Livistona chinensis), christmas palm (Adonidia merrillii), and yellow palm (Chrysalldocarpus lutescens) were analyzed through microscopic observation and phytochemical screening. The results revealed variations in epidermal cell shape, cell wall structure, and stomatal distribution. Most species exhibited smooth-walled epidermal cells, except for red palm (C. renda), which had wavy cell walls. Stomata were predominantly tetracytic, with red palm (C. renda) and christmas palm (A. merrillii) displaying an amphistomatic distribution, while the other species were hypostomatic. Phytochemical analysis revealed the presence of flavonoids and saponins in all samples. Triterpenoids were detected only in phoenix palm (P. roebelenii), fan palm (L. chinensis), and yellow palm (C. lutescens). Alkaloids were found in phoenix palm (P. roebelenii) and red palm (C. renda), while steroids were present in red palm (C. renda), foxtail palm (W. bifurcata), and christmas palm (A. merrillii). Additionally, tannins were detected in all plant samples except for fan palm (L. chinensis). These findings suggest that palm leaves possess significant bioactive compounds that could be explored for pharmaceutical and industrial applications.

Ethnobotanical Review and Potential Diversity of Zingiberaceae Species as Postpartum Medicinal Ingredients in the Interior of Kalimantan Sepsamli, Letus; Kalalinggi, Septaria Yolan; Raihandhany, Reza; Syamswisna; Utami, Widia Sri
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.26048

The local communities in the interior of Kalimantan have long utilized plants from the Zingiberaceae family as traditional medicinal ingredients, especially for postpartum care. Many species of Zingiberaceae are retained in postnatal care because they are effective in alleviating and healing ailments. This study aims to review the potential diversity of Zingiberaceae species used in traditional medicine after childbirth and to identify their chemical compound content. The method used is a literature review with an analysis of scientific literature from the past 10 years discussing the utilization of Zingiberaceae by the community in Kalimantan in managing mothers after childbirth. The results show that there are ten species and four genera of Zingiberaceae that are frequently used, namely Alpinia, Kaempferia, Curcuma, and Zingiber. The plant organ that is utilized is the rhizome, processed in various ways such as boiling, pounding, or turning into powder before being consumed or applied to the body. The compound content in Zingiberaceae includes curcumin, gingerol, and shogaol, which have immunomodulatory, anti-inflammatory, and antioxidant activities, playing a role in postpartum treatment for women. This research emphasizes that Zingiberaceae plays an important role in traditional postnatal treatment, and further studies are needed to support the preservation of local knowledge, as well as conservation and sustainable utilization.

Ethnobotanical Review and Potential Diversity of Zingiberaceae Species as Postpartum Medicinal Ingredients in the Interior of Kalimantan Sepsamli, Letus; Kalalinggi, Septaria Yolan; Raihandhany, Reza; Syamswisna; Utami, Widia Sri
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.26048

The local communities in the interior of Kalimantan have long utilized plants from the Zingiberaceae family as traditional medicinal ingredients, especially for postpartum care. Many species of Zingiberaceae are retained in postnatal care because they are effective in alleviating and healing ailments. This study aims to review the potential diversity of Zingiberaceae species used in traditional medicine after childbirth and to identify their chemical compound content. The method used is a literature review with an analysis of scientific literature from the past 10 years discussing the utilization of Zingiberaceae by the community in Kalimantan in managing mothers after childbirth. The results show that there are ten species and four genera of Zingiberaceae that are frequently used, namely Alpinia, Kaempferia, Curcuma, and Zingiber. The plant organ that is utilized is the rhizome, processed in various ways such as boiling, pounding, or turning into powder before being consumed or applied to the body. The compound content in Zingiberaceae includes curcumin, gingerol, and shogaol, which have immunomodulatory, anti-inflammatory, and antioxidant activities, playing a role in postpartum treatment for women. This research emphasizes that Zingiberaceae plays an important role in traditional postnatal treatment, and further studies are needed to support the preservation of local knowledge, as well as conservation and sustainable utilization.

Antioxidant Activity and Organoleptic Quality of Yellow Marigold Flower Kombucha (Tagetes erecta L.) on the Variations of Sugar Type and Fermentation Duration Yessy, Herviana Yessykasari; Titik, Titik Suryani
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.26086

Kombucha is a fermented beverage made from a mixture of sweet tea using kombucha microbes known as SCOBY (Symbiotic Colony Of Bacteria and Yeast). One of the flowers that has the potential developed as kombucha ingredient is yellow marigold flower (Tagetes erecta L.). The purpose of this study was to determine the antioxidant activity and organoleptic quality of yellow marigold flower kombucha on variations of sugar types and fermentation duration. This study used an experimental method with a Completely Randomized Design (CRD) with 2 factors. Factor I: variation of sugar types (G): palm sugar and Javanese sugar. Factor II: fermentation duration (F): 5 days and 7 days. The results of the study showed that the highest antioxidant activity of yellow marigold flower kombucha was 71.44% in G1F2 treatment (200 g palm sugar and 7 days fermentation duration) and the highest organoleptic quality of yellow marigold flower kombucha was brown color, quite sour taste, quite aromatic type of kombucha and quite liked. in the G2F2 treatment (200 g palm sugar and 7 days fermentation duration).

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Contact Name
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Contact Email
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Journal Mail Official
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Editorial Address
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Location
Kota semarang,

Jawa tengah

INDONESIA