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Biota
Published by Universitas Islam Negeri Mataram
ISSN : 20863586     EISSN : 24608483     DOI : -
Core Subject : Health, Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Medicine & Pharmacology
Focus and Scope of Biota on Bioscience, includes: cell biology and genetics, biodiversity, ecology, physiology and biotechnology. Biota covers all life forms, including microbes, fungi, plants, virus, animal and human. Focus and Scope of Biota on Biology Education, includes: Experimental Research, Class Action Research, Qualitative Research, Quantitative Research, Model Learning Development, Media Learning Development, and Learning Evaluation.
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Articles 137 Documents
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Assessment of the Quality and the Susceptibility Profile of fungi in Peanut Butter Hawked in Lafia Metropolis Orole, Olukayode Olugbenga
Biota Vol 16 No 2 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i2.478

Abstract

Peanuts are a high-nutritional food source containing unsaturated fats, high-quality protein, carbohydrates, minerals and vitamins. The aim of the study was to determine the mycological quality of peanut butter consumed in Lafia and determine the antifungal susceptibility profile of fungal isolates. Peanut butter samples were collected from Lafia markets from which fungi were isolated, and the In vitro antifungal susceptibility profile was determined. The highest fungal count was from Shinge market (28.0x103 cfu/g), and the lowest from the College Village market (3.0x103 cfu/g). Of the fourteen fungal species isolated, genus Aspergillus had four species followed by genera Fusarium with three species. A. flavus was obtained from all the markets while A. carbonarius, A. versicolor, A. parasiticus, Alternaria alternata, Saccharomyces cerevisiae, Candida sp. and Trichophyton verrucossum were found in one market each. Antibiotics sensitivity test showed the highest inhibitory activity against F. graminearum and A. parasiticus from the Roundabout market (100% inhibition). Nystatin was the most effective antibiotic with inhibition of 1.87±0.23 cm against Alternaria alternata, and inhibiting all the isolates, while fluconazole was the least effective antibiotic tested. The study concludes that peanut butter consumed in Lafia has high concentrations of microorganisms making it unsafe for consumption.
Bioremediation potential of tree-level plant vegetation UIN Raden Mas Said Surakarta campus as an alternative solution to environmental problems towards a green campus. Prasetyo, Angga Dwi
Biota Vol 16 No 2 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i2.481

Abstract

Environmental problems become actual and interesting issues to discuss. Environmental problems are not only in water, soil and living things but also include air pollution. One of the environmental solutions can use bioremediation, Educational Institutions can also participate in environmental solutions by becoming a green campus. The purpose of this study is to see the potential of bioremediation of tree-level plant vegetation UIN Raden Mas Said Surakarta as an alternative solution to environmental problems towards a green campus. This research was conducted from July-October 2023, plant classification was carried out thoroughly in the campus area, then a meta-analysis was carried out for its potential as bioremediation and green campus potential. Based on the results of research at the UIN Raden Mas Said Surakarta campus, there are 58 types of plants with a total of 670 plants with the most types of plants red shoots (Syzygium paniculatum Gaertn.) 12% and Ketapang kencana (Terminalia mantaly) 11%. Furthermore, the results of the meta-analysis found that the potential for vegetation bioremediation at UIN Raden Mas Said Surakarta as phytoremediation where plants act as bioremediation agents, while the potential of UIN Raden Mas Said Surakarta as a green campus based on the UI GreenMetric Guidelines 2022 Standards has met several criteria and indicators of green campus achievements, but to become a green campus requires the participation of various parties, both leaders and the academic community and students so that UIN Raden Mas Said Surakarta can get the title of green campus.
Pemanfaatan Jamu Herbal (Sehat Nifas) Dan Air Rebusan (Pinang Muda, Daun Sirih Dan Asam Kandis) Terhadap Percepatan Pemulihan Masa Nifas Early Puerperium Arriza, Nurul; Windiyati, Windiyati; Melyani, Melyani; Handayani, Luluk; BE, Gloria Fortunata; Liza, Erviana; Evi, Evi
Biota Vol 16 No 2 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i2.483

Abstract

Postnatal herbal medicine and boiled water are herbs are usually given to postpartum mothers to strengthen the body and speed up the recovery period of the uterus, speed up the cessation blood and healing perineal wounds. Method: Quasi Experimental research type Pretest-Posttest Nonequivalent Control Group Design. Held at PMB Nuripah Kubu Raya on 21-27 November 2023. Total sample was 34 respondents, the respondents were divided randomly into 2 groups, postpartum mothers in intervention group were given 1x1 multivitamin and postnatal healthy herbal medicine test ingredients at a dose of 1x1 suchet (25 grams) and Postpartum mothers in control group were given 1x1 multivitamin as a comparison, the research was carried out for 7 days. Results: The intervention group had an effect on changes in uterine involution based on anteroposterior diameter (p. 0.005), changes in uterine involution based on longitudinal length (p. 0.002), the amount of locheal discharge (p. 0.003), the smell of locheal discharge (p. 0.002), reducing the pain scale (p. 0.000) and perineal wound healing (0.003). Conclusion: Healthy postpartum herbal medicine and perineal wound fluid effective accelerating early puerperium period (uterine involution, lochea and perineal wounds) with the fastest start of action compared to the control group (multivitamin).
The Concentration Variation Effect of Earring Leaves (Acalypha Indica L.) Extract on It’s Bioactivity Ability in Inhibiting The Growth of Salmonella Typhi (S. Typhi) Ziana, Elma; Furqan, Repika Nurul; Nurul Furqan, Baiq Repika
Biota Vol 15 No 2 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i2.485

Abstract

The research has been carried out on the effect of variations in the concentration of earring leaves (Acalypha Indica L.) ethanol extract on the acquisition of inhibition zone values for the growth of S. typhi bacteria. In this study, ethanol solvent was used in the maceration and evaporation stages, then the well diffusion method was used to test its antibacterial activity. Each stage was carried out in triplo and 3 repetitions. Phytochemical test on the evaporated extract showed positive test results for flavonoid and tannin metabolite compounds. Concentration variations that used in this study were 5%, 15%, 30%, 50% and 60%, the antibiotic chloramphenicol was also used as a positive control and aquadest as a negative control. Meanwhile, the antibacterial activity test gave the result that the higher the concentration of the earring leaf extract (Acalypha Indica L.), the inhibition zone value also increased with the following successive values: 0mm, 11mm, 14mm, 15mm and 16mm, on the other hand chloramphenicol showed an inhibition zone of 32 mm. The research data were analyzed statistically using the One Way Anova test. Earring leaf extract (Acalypha Indica L.) has a significant effect of 0.000 on S. Typhi bacteria. However, at the smallest concentration, namely 5%, it does not have an inhibition zone diameter of 0 mm with a significant value of 1000. It can be concluded that the antibacterial activity using chloramphenicol is still higher when compared to the antibacterial activity using the ethanol extract of earring leaves (Acalypha Indica L.) in inhibiting growth of S. Typhi bacteria.
Test Of Antibacterial Activity Of Ethanol Extract Of Maman Lanang Plant (Cleome Rutidospermae Dc ) Against Gram Positive And Negative Bacteria (Staphylococcus aureus and eschericihia coli) Nurul Furqan, Baiq Repika; Sanjaya, Denny; Almahera, Almahera; Maharani, B Firia
Biota Vol 15 No 2 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i2.486

Abstract

The Maman Lanang plant ( Cleome rutospermae dc ) is a weed plant that is often found in rice fields or grows on community cultivated plants which are usually used as food for people on the outskirts. This research aims to determine the effect of the extract concentration of the Maman Lanang Plant ( Cleome rutospermae dc ) on the inhibition zone for the growth of Staphylococcus aureus and Escherichia coli bacteria, as well as to find out how much the concentration of the extract from the Maman Lanang Plant ( Cleome rutospermae dc ) is close to the inhibitory zone value of the positive control of tetracycline in inhibiting the growth of Staphylococcus aureus and Escherichia coli bacteria . This research is a laboratory experimental study using a Completely Randomized Design (CRD) using the hole/cup diffusion method with four varying concentrations, namely 10%, 30%, 60% and 90%, and the positive controls used are tetracycline and distilled water as a negative control. The main ingredient used in this research was the Maman Lanang plant ( Cleome rutospermae dc ) which was extracted using the maceration method for three days with 96% ethanol solvent, after that the extract obtained was then subjected to an evaporator to obtain the crude extract then carried out a phytochemical test and after that The antibacterial activity of the crude extract from the Maman Lanang Plant ( Cleome rutospermae dc ) was tested against the bacteria Staphylococcus aureus and Escherichia coli . Then the data obtained was analyzed using the antibacterial ANOVA ( Analysis of variance ) method. The results of this study show that phytochemical test analysis shows the presence of flavonoid compounds in extracts from the Maman Lanang Plant ( Cleome rutospermae dc ). Furthermore, the analysis showed that variations in concentration of the Maman Lanang Plant ( Cleome rutospermae dc ) had an influence on the antibacterial activity of Staphylococcus aureus and Escherichia coli. Where the higher the concentration of the extract, the larger the inhibition zone will be formed. The 90% concentration of the extract showed the largest inhibition zone with a diameter of 23.3 mm.
Antibacterial Activity Test of Ethanol Extract of Red Macroalgae (Acanthopora Spicifera) Against Staphylococcus Aureus Bacteria Taibin, Ali; Yamin, Imam Syahputra; Nurul Furqan, Baiq Repika
Biota Vol 15 No 2 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i2.487

Abstract

Bacterial infection Staphylococcus aureus has become a significant global health problem, mainly due to increasing resistance to existing antibiotics. To overcome this problem, research is continuing to find new sources of natural antibacterial agents. Red macroalgae (Acanthopora sp.) has promising biological potential, including activity as an antimicrobial. This study aims to determine the effect and how much concentration of red macroalgae ethanol extract (Acanthopora sp.) which is close to the value of the inhibition zone of the positive control Amoxicillin in inhibiting bacterial growth S. aureus. This research is a laboratory experimental research. The stages of the research began with taking red macroalgae samples (Acanthopora sp.) collected from Pandanan beach, North Lombok Regency, West Nusa Tenggara by the method simple random sampling and identified morphologically, manufacture of simplicia, manufacture of red macroalgae ethanol extract (Acanthopora sp.) with 99.99% ethanol solvent (absolute p.a), phytochemical screening tests, and antibacterial activity tests using a Completely Randomized Design (CRD) were carried out by well diffusion method with concentration variations of 2%, 4%, 6%, 8% and 10%, Amoxicillin as positive control and aquadest as negative control and ethanol as solvent control. Antibacterial activity test results from the crude extract of red macroalgae (Acanthopora sp.) against bacteria S. aureus incubated during overnight (16-18 hours) in an incubator with a temperature of 37OC. The results showed that the phytochemical analysis of the ethanol extract of red macroalgae identified the presence of flavonoids and saponins which are known to have potential as antibacterial agents. The presence of these compounds supports the antibacterial activity of red macroalgae against S. aureus. Red macroalgae ethanol extract (Acanthopora sp.) has antibacterial activity against S. aureus. The antibacterial activity of the ethanol extract showed a concentration-response pattern, where the higher the concentration of the extract, the greater the inhibition zone formed. The concentration of 10% ethanol extract showed the largest inhibition zone with an average diameter of 23.5 mm with the sensitive category.
Bioactivity Diversity (Antibacterial and Antioxidant) of Macroalgae in the Ekas Beach Area, Lombok Nurul Furqan, Baiq Repika; Yamin, Imam Syahputra; Umami, Sri Sofiati
Biota Vol 17 No 1 (2024)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v17i1.489

Abstract

Macroalgae in the southern coastal area of Lombok has not been explored much in terms of bioactivity. The aim of this research is to explore the usefulness of 9 types of macroalgae on Ekas Lombok beach by searching for their bioactivity capabilities, whether each macroalgae has no bioactivity at all, has antibacterial and antioxidant activity, only has antibacterial activity but no antioxidant activity or vice versa and whether there are macroalgae that even have anticancer activity. The method used in this research consists of several steps: Macroalgae Sampling, Morphological Analysis of Macroalgae Samples, Isolation of active compounds from macroalgae and Cytotoxic Test on Breast Cancer Cells in Vitro which consists of 3 tests: Culture and sub culture of MCF-7 Breast Cancer Cells, Cell Viability and Cytotoxicity Test with MTT and DNA Isolation of MCF-7 Breast Cancer Cells. The research results show that the macroalgae with the best antibacterial activity is the red macroalgae Acanthophora spicifera and the macroalgae with the best antioxidant activity is the green macroalgae Ulva reticulata.
Karakteristik Sel Punca Mesenkim yang Berasal dari Tali Pusat (Umbilical Cord Derived Mesenchymal Stem Cell/UCMSC) dari Macaca fascicularis dan Sekretomnya dalam Kondisi Hipoksia Dumingan, Alvian; Malik, Amarila; Rinendyaputri, Ratih; Utama, Hieronimus Adiyoga Nareswara; Sunarno, Sunarno; Purwaningtyas, Yoggi Ramadhani; Idrus, Hasta Handayani; Noverina, Rachmawati; Huda, Fathul; Faried, Ahmad
Biota Vol 17 No 1 (2024)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v17i1.492

Abstract

Mesenchymal Stem Cell (MSC) secretome has potential as a neuroprotective and neuroregenerative agent. It can have effects due to its paracrine factors, such as Brain Derived Neurotrophic Factor (BDNF) and Stromal-Cell Derived Factor-1 (SDF-1) which can be induced with hypoxia preconditioning. This compound may play a role in the treatment of neurological diseases. Stroke has become a neurological disease that contributes to high rates of mortality and morbidity worldwide. There have been several pre-clinical trials on animal stroke models using MSC secretomes from rats and humans, but no studies have been conducted on Non-Human Primate, such as Macaca fascicularis. This species has been widely used in biomedical research and part of it can be utilized for such studies which will reduce the cost of using human MSC. The results of this study, Umbilical Cord (UC)-MSCs of Macaca fascicularis have been successfully cultured and characterized in terms of phenotypic and differentiation. Hypoxia precondition was able to induce BDNF secretion up to 264 pg/mL and SDF-1 up to 666 pg/mL in the UCMSC secretome. Hypoxic preconditioning with 3% oxygen can induce the most optimal BDNF and SDF-1 secretion, compared to 1% and 5% hypoxia.
Comparison of Antioxidant Activity of Methanol Extract of Young and Old Leaves of Avocado (Persea americana Mill.) Pratiwi, Ambar; Agustina, Devanda Rahma; Febrianti, Novi; Eryati, Eri
Biota Vol 17 No 1 (2024)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v17i1.493

Abstract

Avocado plants (Persea americana Mill.) are widely grown in tropical and subtropical regions. Avocado leaves contain natural antioxidants for traditional medicine. This study aims to determine the total phenolic content, total flavonoids, and antioxidant activity values of young and old avocado leaves, and analyze the relationship between total phenol content and total flavonoids to the antioxidant activity value of avocado leaf methanol extract (Persea americana Mill.). The macerated avocado leaf extract was quantitatively tested for antioxidant activity using the DPPH method and the total phenolic and flavonoid contents were calculated using the Follin-Ciocalteu and Follin-Ciocalteu methods, respectively.The results showed that the highest total phenolic content of old avocado leaves was 1.682,27mg GAE/100g dry weight. The highest total flavonoid content of avocado old leaves was 3.858,96mg QE/100g dry weight. Antioxidant activity of methanol extract of young avocado leaves has a value of IC50 321.32 ± 80.43 ppm and avocado old leaves amounted to 288.54 ± 33.06 ppm. Total flavonoid content was more influential on antioxidant activity, with a correlation coefficient of 0.856
The Effect of Using E-modules in Biology Learning on Learning Outcomes of High School and University Students: Meta-Analysis Arifin, Nurfadila; Effendi, M. Harja; Purwati, Nining
Biota Vol 17 No 1 (2024)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v17i1.498

Abstract

This study aims to determine the effect size (ES) of the application of e-modules in biology learning on student's learning outcomes. This study uses the type of meta-analysis research. The research sample consisted of 14 articles sourced from the results of article identification through Google Scholar and Eric. The research data were collected based on the reference of inclusion and exclusion criteria. Data analysis was conducted by calculating the ES value using Cohen’s d formula. The results of data analysis showed that there were 14 articles divided into two levels of education, namely high school (9 articles) and university (5 articles) with a total average effect size score of 1,14 which means the effect size criteria are very large (VL). The ES average score for the high school level is 1,20 or very large (VL) ES average, while the ES average score for the university level is 1,02 or categorized as large (L) ES average. Therefore, it can be concluded that the effect size of the application of e-modules in biology learning has a significant impact on improving student learning outcomes.

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