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Biota
Published by Universitas Islam Negeri Mataram
ISSN : 20863586     EISSN : 24608483     DOI : -
Core Subject : Health, Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Medicine & Pharmacology
Focus and Scope of Biota on Bioscience, includes: cell biology and genetics, biodiversity, ecology, physiology and biotechnology. Biota covers all life forms, including microbes, fungi, plants, virus, animal and human. Focus and Scope of Biota on Biology Education, includes: Experimental Research, Class Action Research, Qualitative Research, Quantitative Research, Model Learning Development, Media Learning Development, and Learning Evaluation.
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Articles 137 Documents
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Hematological Test: Predicting Incidence Dengue Shock Syndrome Yamin, Imam Syahputra; Wahyono, Tri Yunis Miko
Biota Vol 14 No 2 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i2.387

Abstract

Dengue hemorrhagic fever (DHF) is an infectious disease caused by the Dengue virus. DHF cases in Mataram City from 2016 to 2020 tend to increase where most of the DHF patients are treated at the Mataram City Hospital. Death due to DHF infection mostly occurred in patients with Dengue Shock Syndrome (DSS) and mortality from DSS was reported to be 50 times higher than in DHF patients without DSS. Prompt diagnosis and appropriate treatment are important determinants for the prevention and control of dengue cases, so knowing the predictors of risk factors can prevent/reduce deaths from dengue virus. This study used a case-control design. Cases were DHF patients diagnosed with DSS by the treating doctor, while the controls were DHF patients diagnosed not DSS by the treating doctor. The research data was obtained from the results of laboratory tests stored in the medical records of patients treated at the Mataram City Hospital from January 2016 to December 2020. The design of the analysis was aimed at obtaining the odds ratio (OR) value followed by multivariate analysis to determine the risk factors that could detect DSS earlier. The variables that were statistically significant in the prediction of the final model were the increase in hematocrit value > baseline with OR= 17.1 (95% CI: 4.033-72.600), a decrease in platelet value < 100.000/µL with OR = 6 (95% CI). : 2,306-15,699), and decreased leukocyte value < baseline with OR= 5.1 (95% CI: 2,209-11,838). While the most dominant variable is the increase in hematocrit value > baseline with OR = 17.1 (95% CI: 4.033-72.600) and p value = 0.000.
Study of Environmental Parameters on Lobster (Panulirus spp.) Enlargement with Fixed Cage at GWD, Banyuwangi Putra, Riswanda Rangga; Nugraha, Dimas Putra Wahyu; Albarkah, M. Rafly; Ma’arif, M. Chusnan; Fatih, M. Fachrul; Hadi, Moch. Irfan; Violando, Wiga Alif; Nusa, Raras Widya
Biota Vol 15 No 1 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i1.356

Abstract

One of the marine commodities, Lobster (Panulirus spp.), has unabated demand in the global market, while, Lobster production remains static due to the difficulty of implementing its rearing activities. Relative rearing of lobster (Panulirus spp.) is usually performed using the floating net cage method with calm water conditions. In contrast, areas with extreme weather conditions tend to have difficulty applying this method. The potential of using the fixed cage method was assessed by rearing the Lobster at Pesona Bahari Banyuwangi Fish Cultivation Group. This research aimed to investigate the feasibility of the environmental conditions in the waters of GWD, Banyuwangi. The study was conducted for three months. Data such as temperature and light intensity through the help of a pendant was collected. Water quality parameters were recorded during this study, including salinity, pH, dissolved oxygen, and current velocity. Sampling was conducted four times to see the relationship between length and weight. The results showed that the GWD environmental parameters were very supportive for lobster rearing, with temperature values ​​ranging from 27.2oC - 30.1oC, DO around 4.6 mg/l - 5.2 mg/l, salinity 28o/oo - 30o/oo, pH 7 - 8.2, the current speed is 0.468 m/s, and the light intensity is 0 lux - 3,616 lux. The growth pattern of all sampling results except sampling 2 (isometric) has a negative allometric result, with length and weight having a solid relationship. The fixed cage method is considered suitable for lobster rearing activities because the environmental parameters follow their natural habitat and the Fulton condition factor (K) value of more than 1. The greater the value of the Fulton condition factor (K), the healthier the condition lobster.
Making Liquid Sugar Preparations from Lombok-Traditional Sugarcane Juice and Its Potential as Anti-Diabetic Sugar Nurul Furqan, Baiq Repika; Maharani, B Fitria
Biota Vol 15 No 1 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i1.407

Abstract

The prevalence of Patients with Diabetes Mellitus remains on the rise worldwide. Diabetes is a multifactorial disorder that emboils genetic and environmental factors such as an unhealthy diet by consuming white sugar crystals. White crystal sugar consists of less chromium content that induces glucose in the blood to the cells, which can trigger hyperglycemia or high blood sugar levels. Chromium works by increasing the activity of insulin receptors to enhance glucose translocation to the cell. Previous studies showed sugarcane Nira had a high level of chromium compared with those processed into white crystalline sugar. However, it could not be used as a sweetener substitute since its storage age was only 2 to 3 days. This study identified that the treatment process of Lombok's Nira sugarcane into local liquefied sugar should be addressed to maintain chromium levels compared to the processing of Nira into the white crystalline sugar requiring optimum sucrose and omitting several minerals. Hence, sugarcane Nira as a refined sugar product can substitute for white sugar consumption in diabetes patients.
Piper crocatum and Aloe vera Difference Effectiveness in Complementary Treatment of Perineal Wounds Mubayyina, Firdaus; Alam, Gemini; Usman, Andi Nilawati; Ahmad, Mardiana; As’ad, Suryani; Raya, Indah
Biota Vol 15 No 1 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i1.409

Abstract

Indonesia, with a maternal mortality rate (MMR) of 305 per 100,000 live births, is directly caused by post-partum hemorrhage (30.3%) and hypertension (27.1%). At the same time, post-partum infection ranks second as a contributor to maternal death after bleeding. Data from the Indonesian Demographic Health Survey (IDHS) showed that the incidence of perineal laceration or rupture was experienced by women who gave birth vaginally (75%). The prevalence of women who experience perineal tears is in the 25-30 years (24%) and women aged 32-39 years (62%). This study aimed to examine the differences in the effectiveness of Piper crocatum and Aloe vera in the complementary treatment of perineal wound healing in post-partum mothers. This study was a quasi-experimental pretest-posttest control group design with a total sampling method with a ratio of 1:1:1 for as many as 30 post-partum mothers with grade I and II injuries according to inclusion criteria with Intervention group red betel stew, aloe vera, and the Control group with perineal injuries at Gunung Sari Health Center and Sesela Health Center. The data observed were the degree of perineal wound healing using the REEDA scale (Redness, Edema, Ecchymosis, Discharge, and Approximation). The results showed differences in the effectiveness of Piper crocatum and aloe vera on perineal wound healing. The average results of the Mann-Whitney statistical test were on the 5th and 7th days (p-value< 0.005). This study shows that Piper crocatum can accelerate the healing of perineal wounds and become an alternative, complementary therapy in perineal wound healing.
Relative Expression of mRNA BARF1 Epstein-Barr Virus from Tumor Tissue Biopsy in Formalin-Fixed Paraffin-Embedded in Nasopharyngeal Carcinoma Wahyono, Daniel Joko; Fuadi, Muflih; Susanto, Agus Hery; Sulistyo, Hidayat
Biota Vol 15 No 1 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i1.413

Abstract

Nasopharyngeal carcinoma (NPC) is a malignant tumor originating from nasopharyngeal epithelial cells. NPC is endemic in some regions, especially in Southeast Asia countries. NPC is also a multifactorial disease involving environmental factors, genetic factors, and infection from Epstein-Barr virus (EBV). According to WHO classification, Undifferentiated NPC is histopathologically associated with EBV infection and categorized into WHO subtype 3. EBV has two phases in its infection cycle: the lytic and latent phases. The BARF1 gene is a mediator of the transition from the latent phase to the lytic phase. Previous studies suggest measurement of EBV mRNA activity at the primary tumor site in the nasopharyngeal reflects the pathogenesis of NPC rather than measuring circulating EBV DNA or serological diagnosis. This study aimed to determine the relative expression potential of BARF1 mRNA at different tumor stages in NPC patients as a predictor of NPC pathogenesis. This research design was a descriptive research method in the form of a cross-sectional study. The samples used were 22 patients diagnosed as NPC WHO class III at the Anatomical Pathology Section of Prof. Dr. Margono Soekarjo, Purwokerto, who met the inclusion criteria. The relative expression of BARF1 mRNA was carried out using the one-step real-time RT-PCR technique and then calculated using a formula of 2-ΔCt. The T-test was used to compare the relative expression of early and late-stage BARF1 mRNA. The relative expression of BARF1 mRNA in the late-stage advanced stage (n = 6; 0.708292-0.840177; med = 0.7164655) was increased compared to the early stage (n = 2; 0.708841-0.712423; med = 0. 710632).
Antibacterial Potention and pH Analysis of Kombucha with Anna Apple (Malus domestica) Peel as Its Substrate Lestari, Kinanti Ayu Puji; Wulansari, Silvi Ayu
Biota Vol 15 No 1 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i1.363

Abstract

This study aimed to determine the antibacterial abilities of Kombucha with Anna apple peel as the substrate. This study was conducted on the antibacterial assay of Kombucha with the formulation of 10g (formulation A), 15g (formulation B), and 20g (formulation C) apple peels. Each formulation then made three different test concentrations of an antibacterial test by the Kirby Bauer method. There was a decrease in the pH value measured before and after fermentation of kombucha apple peel due to the production of acetic acid formed from metabolic activity between bacteria and yeast as a culture of kombucha beverage. The results of the antibacterial test showed that all of the test concentrations could inhibit the growth of the test bacteria until they were in the category of medium and high inhibition. The largest diameter of the inhibition zone was shown by formula C with a concentration of 100% (17 mm). The diameter of the inhibition zone is directly proportional to the amount of apple peel contained in the formula and the concentration of the sample being tested.
Comparison of the Effectiveness of Piper Crocatum and Sour Turmeric Acid for Healing Time Lestari, Dewi; Usman, Andi Nilawati; Alam, Gemini; Hadju, Veni; Husin, Farid; Sartini, Sartini
Biota Vol 15 No 1 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i1.412

Abstract

Maternal mortality (MMR) is still 359 per 100,000 live births, and the expected target is still far from what was proclaimed in 2030, the number of 70 per 100,000 live births. Based on data from WHO (Word Health Organization), in 2015, the deaths were around 2.7 million cases caused by perineal injuries. According to IDHS data (Indonesian Demographic and Health Survey), in 2017, the maternal mortality rate (MMR) was 305 per 100,000 live births caused by an infection in the perineal wound. The total maternal mortality rate (MMR) in various provinces in Indonesia has decreased from 4,226 to 4,221. In 2019 the maternal mortality rate (MMR) was most contributed by bleeding (30.3%) and postpartum infection (5%). This study aimed to determine the differences in the effectiveness of red betel and turmeric acid in the complementary wound healing treatment in postpartum women. This study was a quasi-experimental pretest-posttest control group design with a total sampling method by giving three intervention and control groups, as many as 30 postpartum women with perineal wounds grades I and II according to the inclusion criteria with the group given red betel, tamarind, and turmeric. Control group at Anutapura General Hospital Palu. Perineal wound care using the REEDA scale (Redness, Edema, Ecchymosis, Discharge, and Approximation). The results showed a difference in the effectiveness of red betel and turmeric acid on perineal healing. The average Mann-Whitney statistical test results were on the 5th and 7th days (P-Value < 0.005). This study shows that Piper crocatum can accelerate perineal wound healing and become an alternative for complementary therapy and perineal wound healing.
The Genetic Diversity of Macroalgae on The Ekas Coast of East Lombok Based on Chromatogram Data from PCR Ampification Baiq Repika Nurul Furqan; Imam Syahputra Yamin; Sri Sofiati Umami
Biota Vol 16 No 1 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i1.417

Abstract

Macroalgae are multicellular photosynthetic organisms that live in water. Some species of macroalgae are very difficult to identify based on their morphology alone because they have great similarities. Therefore, identification of macroalgae diversity based on its genotype is very important to do in order to enrich the genetic information of macroalgae found on one of the beaches on the island of Lombok. This study aimed to analyze the genetic diversity of macroalgae from Pantai Ekas Beach, Jeowaru District, East Lombok, NTB using 18S rRNA gene markers. The research was carried out in 4 stages, namely sampling, morphological analysis of samples, isolation of total macroalgae DNA, isolation of genes by PCR amplification, agarose gel electrophoresis. Sampling was carried out at certain coordinates at east longitude and south latitude using the Ekas Beach quadratic transect method. The morphological analysis of the samples was guided by an identification key. Total DNA, macroalgae were isolated based on the spin column I DNA kit easy method. Genes were isolated by means of PCR amplification. Total DNA isolates and gene amplicons were confirmed by agarose gel electrophoresis. The results obtained were based on their morphology, all samples obtained were grouped into 9 macroalgae samples which could be divided into brown macroalgae (3 samples), green macroalgae (2 samples) and red macroalgae (4 samples). All samples have successfully isolated total DNA and its 18S rRNA gene which was confirmed by agarose gel electrophoresis chromatogram.
Inggris Subhan Maulidi Kun Bad'a Farohii; Fatchur Rohman; Sulisetijono Sulisetijono
Biota Vol 16 No 1 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i1.420

Abstract

Research on the characterization of potential perennials to resist river erosion in the Brantas river border based on different environmental features in Malang, East Java has been carried out. The research aims to know the composition of plant species, plant diversity, and the dominance of perennials that can prevent erosion. The study used vegetation analysis with cruise methods, exploring perennials on the Brantas river border based on different environmental settings (residential industry, environment, fields). The results of the study found 49 species of plants in 25 families, the diversity of hard plants in the residential environment was high with H' 3.06 while in the industrial and rice fields/fields environment it was moderate with H' 2.67 (Industry) and H' 1.90 (fields). The dominant plants are Gigantochloa apus (Schult. f.) Kurz ex Munro (Industry) species, Calliandra houstoniana (Mill.) Standl. (Settlement) and Pinus merkusii Jungh. & Vriese ex Vriese. (fields) Many perennials in the study area can prevent erosion because these plants meet the criteria of a strong root system and economic value. Keywords: Perennials, Erosion, Border, Brantas River
Optimization of Biodiesel Production from Sludge of Mask Waste and Used Cooking Oil Using Lipase as Biocatalyst Muhammad Rizal Januardi; Baiq Repika Nurul Furqan
Biota Vol 16 No 1 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i1.423

Abstract

Mask waste and used cooking oil contain hydrocarbons and free fatty acids. the combination of these ingredients with pretreatment heating in the microwave and the help of lipase enzymes makes the resulting sludge has the potential to be converted into biodiesel with a maximum yield when compared to making biodiesel from other materials and methods. The results of this study indicate that pre-treated sludge from mask waste and used cooking oil can be converted into biodiesel through a methanol transesterification reaction with the help of thermostable lipase enzymes from Pseudomonas. As for the optimum conditions, the highest biodiesel yield was produced at a temperature of 60℃, the best reaction time was 25 minutes, the volume of sludge was 10 mL and the optimum pH was at pH 8. The flame test on the biodiesel produced had been carried out, most of which had bright blue, red flames and orange. There are also some results that the flame is dim and even the test results are not lit.

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