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Biota
Published by Universitas Islam Negeri Mataram
ISSN : 20863586     EISSN : 24608483     DOI : -
Core Subject : Health, Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Medicine & Pharmacology
Focus and Scope of Biota on Bioscience, includes: cell biology and genetics, biodiversity, ecology, physiology and biotechnology. Biota covers all life forms, including microbes, fungi, plants, virus, animal and human. Focus and Scope of Biota on Biology Education, includes: Experimental Research, Class Action Research, Qualitative Research, Quantitative Research, Model Learning Development, Media Learning Development, and Learning Evaluation.
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Articles 137 Documents
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Stomata Profile Comparisons in Abaxial and Adaxial Zones of Dendrobium aphyllum and Arachnis flos-aeris Leaves Febri Yuda Kurniawan; Agung Dwi Santoso
Biota Vol 13 No 2 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i2.310

Abstract

Stomata activity in orchids is related to orchids' adaptation process to their environment. This study aimed to investigate the stomata profile, comprising of anatomical structure and density of the two orchids species, Dendrobium aphyllum, and Arachnis flos-aeris. Data was collected by printing the leaves' surface using glue and observed at 09.00 am and 09.00 pm, then analyzed using Image raster 3.0 software and MS. Excel. The stomata of D. aphyllum and A. flos-aeris were characterized by anomocytic type. Results showed that the highest stomata density was 1177.78 um2, found on the abaxial part of D. aphyllum. The adaxial leaves of both species were classified as low or no stomata. These two orchid species were epiphyte yet grow in different habitats. Arachnis flos-aeris exposed to direct sunlight tends to reduce transpiration rate, resulting in fewer stomata than D. aphyllum less exposed to sunlight.
Antimicrobial Resistance and Molecular Identification of Clinical Multi-Drug Resistant Enterobacter Cloacae Fajriah, Siti Nur; Inggraini, Maulin; Ilsan, Noor Andryan; Anindita, Reza
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.334

Abstract

Enterobacter cloacae is a Gram-negative bacteria causing nosocomial infections. This bacteria has increased resistance to various antibiotics in the past five years, resulting in a multi-drug-resistant (MDR) phenotype. In particular, MDR E. cloacae causes longer hospitalization time, increases medical costs, and affects morbidity and mortality. This study aimed to observe the minimum inhibitory concentration (MIC) of clinical E. cloacae towards several antibiotics and molecular identification of MDR E.cloacae. This study was conducted in a descriptive design. Secondary data was collected at the microbiology laboratory of the Teaching Hospital in Bekasi, Indonesia, from May to September 2020. Sampel was carbapenem resistant E.cloacae. The isolate was originated from a human clinical specimen, then was confirmed molecular identification using 16s rRNA. In this study, only one carbapenem-resistant E. cloacae, which is also MDR bacteria, was found. This E. cloacae was categorized as MDR bacteria since it was resistant to more than three antibiotic classes, including carbanemen, extended-spectrum cephalosporin, penicillins + β lactamase inhibitor, antipseudomonal penicillins + β lactamase inhibitor aminoglycoside, and penicillin. Vitek 2 identification of this isolate was E. cloacae complex. It showed similar results to molecular identification based on a partial sequence of 16s rRNA. BLASTn result of the trimmed sequence was E. cloacae with 99.78 % similarity.
pengaruh kombinasi ekstrak benalu teh dan mangga terhadap profil histopatologi tikus hipertensi (DOCA-SALT) Oktaviana, Nimas Ajeng; Abdoes Sjakoer, Nour Athiroh; Mubarakati, Nurul Jadid
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.339

Abstract

The purpose of this study was to analyze the brain histopathological profile in hypertensive rats (DOCA-Salt) treated to Tea and Mango extract combination (Mistletoe). Samples were obtained using a true experimental design completely randomized on 25 rats with three treatments (TI, TII, TIII), negative control, and positive control with replication five times. Ratio of extract for treatment was 3 : 1 made up 50 mg/kg BW, 100 mg/kg BW, 200 mg/kg BW. Data analysis using one-way analysis of variance (ANOVA) with the JAMOVI application. The study results obtained Mistletoe (Tea and Mango) extract combination in hypertensive rats (DOCA-Salt) can significantly reduce the necrosis of brain cells in the white matter area. The combination of Mistletoe extract can reduce the number of brain cell necrosis in hypertensive rats optimally obtained at a dose of 50 mg/kg BW
Kelimpahan Mikroba Tanah Asal Rizosfer Tanaman Sukulen Zulaika, Aidha; Wijayanti, Dian Rachma; Safari, Wulan Fitriani
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.340

Abstract

Plant host symbiosis is close related to soil microbial abundance. Soil microbial abundance will affect soil quality and fertility, thus will improve plant growth. Studies on soil microbial abundance in succulent plant rhizosphere, especially in Indonesia, are scarce. This study aims to observe soil microorganisms' existence and their abundance on succulent plant rhizosphere. This research used two primary methods to isolate Arbuscule Mycorrhizal Fungi (AMF) and actinomycetes. The spore extraction (soil separating) method was used to obtain AMF spores, followed by the root staining method to observe AMF infection on the plant roots. Serial dilution and pour plate method were used for isolation of Actinomycetes. The isolation results showed a high number of Actinomycetes distribution up to 3.3 x 106 Actinomycetes CFU/g from the plant Echinocactus grusonii, while AMF spores displayed the most elevated number up to 47 spore/25g from the plant Deuterocohnia sp. The percentage of AMF root infection is covered by 27,9% median. AMF identification was based on spore. The result showed an abundance presentation of Actinomycetes in each soil sample. AMF and Actinomycetes existence displayed symbiotic interaction between succulent plants and soil microorganisms. AMF and actinomycetes play the role of endophytes that help the growth of cacti, generally have a dry growing environment, and limited nutrition by this symbiotic interaction. Actinomycetes distribution in rhizospheric soil will increase plant growth-promoting factors activity. Eventually, the implications of research results are to explore the abundance and biodiversity of soil microorganisms from succulent plant rhizosphere that lacked exploration.
Polyploidy Induction of Rutaceae through Bio-catharanthine Treatment Aziz, Isna Rasdianah; Muthiadin, Cut; Hajrah, Hajrah; Alir, Rahmat Fajrin; Suryafly, Fanny Dhea; Amnah, Alifah Zul; Hermawan, Isna Ainun; Mustami, Muhammad Khalifah; Mahfut, Mahfut; Upreti, Brij Mohan
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.344

Abstract

Indonesia has a tropical monsoon-type climate which suitable for agriculture and plantations. One of the main centers of orange fruits (Citrus sp.) production in Indonesia and become the top ten with the largest production is in South Sulawesi. Farmers commonly cultivate siam oranges (Citrus nobilis). Lime (Citrus aurantifolia) has not yet become the main production, which is inversely proportional to the high demand in South Sulawesi. This study aims to determine the ability of bio-catharanthine as a polyploidy agent against the phenotype of the familia Rutaceae cultivated in South Sulawesi. The research was carried out in Laboratory of Genetics and Molecular Biology, Department of Biology, Universitas Islam Negeri Alauddin Makassar using randomized complete block design consisted of two conditions (bio-catharanthine concentration including C1 = Bio-catharanthine 0.05%; C2 = Bio-catharanthine 0.075%; C3 = Bio-catharanthine 0.1% and immersion time including T1 = 3 h; T2 = 6 h). Phenotypic observations include plant height, the number of leaves and nodes, the length of roots, and the number of roots per shoot. The results of the study showed that the bio-catharanthine immersion of 0.05-0.075% for 6 hours in Rutaceae had a significant effect on their phenotypic characters.
Comparison of the Chaetodontidae Mitochondrial Genome Ferdyan, Rhavy; Razak, Abdul; Ahda, Yuni
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.341

Abstract

The mitochondrial genome is useful for identification because the mutation rate of mtDNA is higher than nDNA. Previous studies have carried out many analyses on the mitochondrial genome of several species, the Chaetodontidae group. Fish in this group are biological indicator fish of coral reefs, so their molecular characteristics must be enriched to conserve these fish. This article aims to review several forms of the mitochondrial genome of the Chaetodontidae group of species, including: Chaetodon speculum, Chaetodon octofasciatus, Chaetodon wiebeli, and Heniochus chrysostomus. Data were collected qualitatively by reviewing previous research articles related to the Chaetodontidae mitochondrial genome. The review focused on the characteristics of each species' mitochondrial genome, including length, overall composition, tRNA, and non-coding region. Each species exhibits various features of the mitochondrial genome and is mapped in its phylogenetic analysis.
The Ethnobotany Study of Medicinal Plants in Lombok Island Damayanti, Riska; umami, sri sofiati; Suhirman, Suhirman
Biota Vol 14 No 2 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i2.386

Abstract

Lombok has the biodiversity plant potential that can be used as medicine. This study aimed to identify the types of medicinal plants used by Sasak people, how to obtain, the parts used, how to use and processing, and the Species Use Value (SUV), Family Use Value (FUV), and Fidelity Level (FL). The results showed that there were 84 species used as traditional medicine by Lombok people. The method of obtaining medicinal plants was mostly done by cultivation, as much as 58% of the processing methods, and the most common use of plants in medicine was boiling and drinking. Plant species that had the highest SUV value were Morinda citrofolia, Zingiber officinale, and Elephantopus scaber with SUVs of 0.8 each. The plant family that had the highest FUV value was Zingiberaceae (eight species), furthermore, the part of the plant that had the highest value was the leaf. The total of plant species that had the highest Fidelity level was 52. One of them was celery (Apium graveolens L.). The data obtained from this research was needed to create an inventory of medicinal plant species and their use by the community, so that traditional knowledge of medicinal plants could be documented and preserved.
Studi Etnobotani Mahoni (Swietenia mahagoni) di Desa Cowek Kecamatan Purwodadi Kabupaten Pasuruan Hildasari, Nourma; Hayati, Ari; Zayadi, Hasan
Biota Vol 14 No 2 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i2.351

Abstract

Mahogany (Swietenia mahagoni) is a plant that has many benefits ranging from stems, leaves, tree bark, fruit and seeds. The purpose of this study was to explore public perceptions regarding aspects of the utilization and distribution of mahogany in Cowek Village, Purwodadi District, Pasuruan Regency. The method used is descriptive exploratory which consists of: literature study, observation, interviews, data analysis using a questionnaire that has been tested for validation and reliability. Utilization among others, in the hamlet of Selowinangun, mahogany is used for 10% protection plants and 90% plant cultivation. In Krajan hamlet, mahogany is used as 20% medicine, 35% as a protective plant, 25% firewood and 20% animal feed. Then in the hamlet of Putuk as a protective plant 15% and building materials 85%. In Sumbersari hamlet, mahogany is used as 50% furniture, 40% building material, and 10% protective plant. In the Sempu hamlet itself, mahogany is used as a 50% protection plant, 25% medicine, and 25% animal feed. The most distribution of mahogany plants is in Sempu Hamlet with 13 location points, Krajan Hamlet with 10 location points, Selowinangun Hamlet with 8 location points, while in Putuk Hamlet and Sumbersari Hamlet there are 5 location points.
Growth and Mortality Rate of Scad (Decapterus macrosoma, Bleeker 1851) landed at Inengo Fish Landing Base, Bone Bolango, Gorontalo Pasisingi, Nuralim
Biota Vol 14 No 2 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i2.362

Abstract

This study aimed to determine the growth pattern and mortality of Scad (Decapterus macrosoma) landed at Inengo Fish Landing Base. A total of 400 fish samples were obtained from the catches of fishers who landed their catch at the landing base using the layered random sampling method. Sampling was carried out from February to March 2021 by measuring the length and weight of the fish samples. Data were analyzed using Microsoft Excel and the FiSAT II application. The results showed that the growth pattern of D. macrosoma was positive allometric with the asymptotic length (L∞)= 279.54 mm, the coefficient of growth rate (K)= 0.49, and the theoretical age of fish (t0)= -0.18 years. The fishing mortality (F) was 2.44 per year; the natural mortality (M) was 0.43 per year; the exploitation (E) was 0.85.
Community Structure of Dragonfly (Anisoptera) and Damselfy (Zygoptera) at The Gunung Sigogor Nature Reserve, East Java, Indonesia Susanto, Muhamad Azmi Dwi; Priambodo, Bagus; Firmansyah, Richo; Pranata, Dicky Candra; Aninnas, Afina Nur; Aji, Fajar Dwi Nur; Widodo, Tri Wahyu; Gunawan, Gunawan; Permana, Danafia; Adiba, Faisal Yanuar; Ristanto, Yuri; Erfanda, Muhamad Prayogi; Kadafi, Ahmad Muammar
Biota Vol 14 No 2 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i2.364

Abstract

Gunung Sigogor Nature Reserve is a conservation forest administratively located in Ngebel District, Ponorogo Regency, East Java, Indonesia. The dragonfly and damselfly community structure, which includes diversity, abundance, evenness and dominance, can be used as an early indicator of changes in environmental conditions, especially in aquatic ecosystems. This study aimed to determine dragonfly and damselfly’s community structure, diversity, evenness, dominance, and habitat preference. This study uses the Visual Encounter Survey (VES) observation and transect methods. This method is carried out by tracing all predetermined observation stations by recording the diversity of dragonfly species and counting the number of individuals. The study conducted in the Gunung Sigogor Nature Reserve area showed 15 species from 8 families consisting of 9 species of Suborder Anisoptera and six species of Suborder Zygoptera. In the results, the highest diversity index value was in the Small Stream (S3) with H'= 1.84. The evenness index, Goa Jepang Stream (S5), has the highest value with a value of E=0.9, and the dominance index of Watu Bandar Stream (S6) location has the highest value with a value of C=1.

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