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Jurnal Biomedika

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Published by Universitas Setia Budi

ISSN : 20892136 EISSN : 23021306 DOI : -

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

JURNAL BIOMEDIKA (p-ISSN:1979-035X, e-ISSN: 2302-1306) is a scientific publication media that accommodates the scientific creativity of lecturers and researchers as outlined in scientific writing, both for academics in Setia Budi University and observers of medical biology and health. Articles published are the results of research in the field or in laboratories, scientific studies and studies of books that have never been published in other media. Information on scientific works can range from biotechnology, health, pharmacology, microbiology, biochemistry, and food analysis and health management.

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Articles 199 Documents

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Analisis Pengawet Natrium Benzoat pada Selai Stroberi Curah di Pasar Tradisional Chintya Putri Wira Dhika Luwitono; Petrus Darmawan
Biomedika Vol 12 No 2 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Sodium benzoate is an artificial preservative that works to make food last longer. The maximum limit of the use of sodium benzoate on fruit jam according to SNI 01-0222-1995 is 1 g/kg. Strawberry jam in the market is possible to use preservative sodium benzoate that exceeds the quality standard so an analysis is needed. Determination of preservative levels of sodium benzoate in strawberry jam according to SNI 01-2894-1992 using the alkalimetry method. The principle of this method is the neutralization reaction between acids and bases, where H+ ions from acids will react with OH- ions from bases to form a neutral salt. The filtrate resulting from the extraction of the strawberry sample is evaporated on a water bath and the residue obtained is dried in a desiccator. The residue was dissolved in neutral 70% ethanol and added with a 1% PP indicator solution. The solution is then titrated with a standard NaOH solution. The end point of the titration is marked with a constant pink color. Based on the results of the study, there were 10 samples of bulk strawberry jam sold in several traditional markets in Jebres sub-district, Surakarta, where 8 samples were positively containing sodium benzoate. The preservative concentration of sodium benzoate in the A1 market sample is 1.327 g/kg, market sample A2 is 1.490 g/kg, market sample B1 is 1.030 g/kg, market sample B2 is 1.451 g/kg, market sample C2 is 1.444 g/kg, E market sample is 1.077 g/kg, F1 market sample is 1.431 g/kg and F2 market sample is 1.635 g/kg. When compared with the maximum limit of the use of sodium benzoate, bulk strawberry jam sold in several traditional markets in Jebres sub-district Surakarta which is positive sodium benzoate does not meet the requirements of SNI 01-0222-1995.

Perbedaan Derajat Aglutinasi Uji Golongan Darah Berdasarkan Teknik Penanganan Sampel dalam Pembuatan Suspensi Sel Darah Merah Lucia Sincu Gunawan; Rumeyda Chitra Puspita
Biomedika Vol 12 No 2 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Pre transfusion testing is a very essential part of blood transfusion procedure. In some types of pretransfusion test, suspension of red blood cells is required. This cell suspension is made to optimize the antigen-antibody reaction so that the on going reaction can be clearly observed. The practice of determining blood type needs a suspension of red blood cells, which its washing phase is a time-consuming. The type of sample used can be whole blood with anticoagulants or frozen blood samples. Due to time constraints, the standard procedures were modified. This study aimed to determine the differences in the degree of agglutination of blood type testing based on the handling samples’ techniques in the manufacture of red blood cell suspensions. Blood group testing was done by the tube method on venous blood samples with different sample handling techniques: frozen blood samples without anticoagulants with storage, fresh blood samples with direct addition of 0.9% NaCl, and EDTA anticoagulant blood samples with refrigerator storage 8 - 24 hours at 40C. The number of samples for each type of treatment is 30 samples, and an assessment of the degree of agglutination in the blood group test is carried out by 2 analysts. There were differences in the mean degree of aglutination between the 3 suspensi-making techniques with NaCl samples (Mean = 3.88; SD = 0.32), frozen samples (Mean = 3.93; SD = 0.25), and EDTA samples (Mean = 3.98; SD = 0.13), but the mean differences was not statistically significant (p> 0.05), so it can be concluded that the three types of samples could be used to replace one another as a comparable sample alternative in making red blood cell suspension to measure ABO cell grouping method.

Aktivitas Antioksidan dan Kadar Vitamin C Daging Buah dan Sirup Buah Naga (Hylocereus costaricensis) Titin Aryani; Isnin Aulia Ulfah Mu’awanah
Biomedika Vol 12 No 2 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Research on antioxidant activity and vitamin C levels of dragon fruit and Hylocereus costaricensis dragon fruit syrup from Kulon Progo has been conducted. The purpose of this study was to study the antioxidant activity and levels of vitamin C in dragon fruit meat and dragon fruit syrup from Kulon Progo. The sampling method is done by purposive sampling method. The method of measuring antioxidant activity using DPPH (1,1-Diphenyl-2-picryl Hidrazil) immersion method was measured with a wavelength of 517 nm. The method of determining the level of vitamin C is carried out by the method of titration of Iodimetry using a standard solution of iodine. The results showed that the average antioxidant activity of dragon fruit was 67.81%, whereas in dragon fruit syrup was 42.81%. While the level of vitamin C in dragon fruit is 12.65% while in dragon fruit syrup is 3.667%. Can reduce the levels of antioxidants and vitamin C levels of dragon fruit higher than dragon fruit syrup.

Perbedaan Jumlah Retikulosit Sebelum dan Sesudah Pemberian Tablet Tambah Darah Zefika Lutfi Ivana; Lucia Sincu Gunawan
Biomedika Vol 12 No 2 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Anemia is a condition where the number of red blood cells in the body is not fulfilled. Iron deficiency anemia in young women has to be taken seriously because it can cause the development disturbance. Reticulocyte is a parameter commonly used to determine the success of therapy in iron deficiency anemia, which showed the body’s physiologic response by enhancing the red blood cells production. This study aimed to find out the differences of reticulocyte count before and after administration of iron tablets on female students at Setia Budi University Surakarta. Using Randomized Control Trial, this study separated the subject randomly into control group and treatment group which received iron tablets for 7 days. The subjects were 40 female students at Setia Budi University in Surakarta. Reticulocyte count examination was carried out at the Setia Budi University Hematology Laboratory, using manual calculations with Brillant Cresyl Blue supravital staining. Data normality was tested with Saphiro Wilk and Independent t test were performed before and after 7 days administration of iron tablets between 2 groups. Reticulocyte count before supplementation in the treatment and control groups showed no significant difference (p = 0.084). After administration of iron tablets, reticulocyte count showed a significant difference (p = 0.005) between the control group and the treatment group. Effect Size exhibited p = 1.509 which meant that the effect of iron tablets supplementation was quite large. This study concluded there were significant differences in the reticulocytes count before and after the administration of iron tablets to the students of Setia Budi University, Surakarta. It is very important to take iron tablets regularly at recommended dose to prevent anemia in women of childbearing age.

Deteksi Diarrhoegenic E. coli pada Sampel Feses Penderita Diare di Puskesmas Batulicin dan Pagatan Kabupaten Tanah Bumbu dengan Metode Polymerase Chain Reaction (PCR) Ika Setianingsih; Dicky Andiarsa; Erli Hariyati
Biomedika Vol 12 No 2 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Diarrhoegenic E. coli (DEC) is a group of pathogenic E. coli strains known to be one of the causes of diarrheal disease. Diarrhea cases are still quite high in developing countries like Indonesia. Tanah Bumbu Regency, especially Batulicin and Pagatan, have a fluctuating rate of diarrhea. This study aims to detect DEC in diarrheal patients in the work area of Batulicin and Pagatan Health Centers using the PCR examination method. Cross Sectional research design with a total sample of 15 respondents collected for 3 months (August - October 2019). Stool samples were examined using the PCR method to detect the presence of DEC strains namely EAEC, ETEC, and EPEC and EHEC. Three out of fifteen (20%) samples were known to be positive for Diarrhoegenic E. coli (DEC). ETEC strains were found in 2 (0,13%) sample, each from the Batulicin Health Center and the Pagatan Health Center, while EPEC and EAEC strains were found in the same 1 (0,07%) sample from the Pagatan Health Center. EHEC strain was not detected in all samples. Diarrhogenic E. coli strains found in this study are EAEC, ETEC, and EPEC. However, it cannot be ascertained yet that the three strains are the cause of the diarrheal disease, it needs to be further to do comprehensive research. The community should be able to increase awareness of the importance of clean and healthy behavior, especially food and beverages consumption, so it can reduce the number of diarrhea cases.

Uji Efektivitas Ekstrak Etanol Daun Bugenvil (Bougenvillea Spectabilis) terhadap Penurunan Kadar Kolestrol Total Mencit Yang di Induksi Pakan Tinggi Lemak Rifda Naufa Lina; Sofiyatul Nurul Jannah
Biomedika Vol 12 No 2 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Cholesterol is a natural substance with fat-like physical properties that has a steroid group. The increase in cholesterol causes deposition in the walls of blood vessels which can cause narrowing and hardening of the arteries or atherosclerosis which can increase the risk of coronary heart disease (CHD). Flavonoids, alkaloids, steroids, and tannins in bougainvillea leaves are thought to reduce total cholesterol levels. This study aims to determine the effectiveness of bougainvillea leaf ethanol extract (Bougainvillea spectabilis) to decrease the total cholesterol level of mice (Mus musculus) induced by high-fat diet for 21 days. This study true experimental using 25 male mice (Mus musculus) divided into 5 groups.. In the dose I, II, III treatment group were given bougainvillea leaf ethanol extract with doses of 40 mg/kgBW, 80 mg/kgBB and 160 mg/kgBB, negative controls were given CMC-Na and positive controls were given simvastatin. Giving is done orally for 14 days. The results of the study were analyzed using One-Way ANOVA analysis test and the results obtained p=0,000. Furthermore, the Post Hoc Tukey test was carried out, the results of which were not significantly different between positive controls and dose III. So, the effective dose of ethanol extract of bougenvillea leaves in decreasing total cholesterol levels in mice is dosis III (160 mg/kgBB).

The Effect Pengaruh Mengkonsumsi Sari Kurma dan Buah Naga dengan Peningkatan Kadar Hemoglobin untuk Pencegahan Anemia Liss Dyah Dewi Arini; Nabulatul Fanny; Devi Pramita Sari
Biomedika Vol 12 No 2 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v12i2.610

The World Health Organization (WHO) stipulates that anemia criteria use hemoglobin levels, namely in women under 12 g / dL and in men less than 13 g / dL. Anemia level someone is influenced by the food they consume, where a person consumes foods that have a low Fe content will occur anemia. Hemoglobin is part of the blood which has an important role in forming red blood cells. Iron is needed in the process of blood formation. Copper substances contained in palm juice are needed by the body to form red blood cells. Red blood cell production is small, red blood cell damage and blood loss can cause a person suffering from anemia. Sari dates and dragon fruit are rich in iron which is useful for forming red blood cells. The purpose of this study was to determine the effect of dates palm juice and dragon fruit in increasing hemoglobin levels. The population in this study is the same as the sample, namely 40 female workers at the Surakarta Gelora convection, which were divided into two groups: 20 people (group 1) who were given palm juice and 20 people (group 2) were given dragon fruit. The tool used in this study is a measure of hemoglobin levels in the blood, check list. The conclusions of this study were the average pre-test hemoglobin levels by giving palm juice of 10.61 and the post-test average hemoglobin level of 11.53 and the average hemoglobin level before and after being given dragon fruit by 10.42 and the average average post-test hemoglobin level of 10.62.

Aktivitas Antibakteri Kombinasi Minyak Atsiri Daun Kemangi (Ocimum Basilicum L.) dan Daun Jeruk Purut (Citrus Hystrix D.C.) terhadap Escherichia Coli ATCC 25922 Fitri Jati Rukmana; Reslely Harjanti; Dyonisius Andang Arif Wibawa
Biomedika Vol 12 No 2 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Basil essential oil has a component of linalool compounds belonging to terpenoid derivatives which have an antibacterial effect and essential oils of kaffir lime have citronella compounds which can inhibit the growth of various types of bacteria. This study aims to determine the antibacterial activity of essential oils of basil leaves, kaffir lime leaves and the combination of both against Escherichia coli bacteria ATCC 25922. This study has used diffusion methods to test antibacterial activity. The concentration of the sample was 2% and 4% with a ratio combination of essential oils were 1:1; 1: 2; 2:1. Then the research data were analyzed with two way Analysis of Variance (ANOVA). The results showed that essential oils in the combination of basil leaves (Ocimum basilicum L.) and kaffir lime leaves (Citrus hystrix D.C) had antibacterial activity against Escherichia coli ATCC 25922 with concentrations of 2% and 4%. Variations of essential oils from basil (Ocimum basilicum L.) leaves and kaffir lime (Citrus hystrix D.C) leaves with a ratio of 1:2 at a concentration of 4% has the greatest inhibitory potential against Escherichia coli ATCC 25922 with inhibition diameters of 15.5 mm.

Uji Aktivitas Antibakteri Ekstrak Etanolik Kayu Secang (Caesalpinia sappan L.) terhadap Staphylococcus aureus Diah Mukti Cahyaningtyas; Nony Puspawati; Rinda Binugraheni
Biomedika Vol 12 No 2 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Piogenic infection is an infection characterized by the occurrence of severe local inflammation with pus formation (pus). Generally caused by piogenic germs, one of the most common is Staphylococcus aureus. One of the plants used as an ingredient in traditional medicine and used as an anti-bacterial is a secang plant. This study aims to determine the anti-bacterial activity of ethanolic extract of secang wood (Caesalpinia sappan L.) on pure cultivation Staphylococcus aureus Laboratory and isolates pus Hospital patients and find out the difference in inhibition between the two bacterias.300 grams of secang wood powder was extracted by maceration method using 70% ethanol (1:10). Maserate was used to test the anti-bacterial activity of Staphylococcus aureus from pure cultivation laboratory and isolates of pus patients in hospital using diffusion and dilution methods. Then, the results of the diffusion test were analyzed using a two-way Anova test.The results of the study showed that ethanolic extracts of wood have anti-bacterial activity. Result of dilution method showed the value of KBM at a concentration of 3% for Staphylococcus aureus cultivation Laboratory and a concentration of 4% for Staphylococcus aureus isolates pus patients of the Hospital. The statistical analysis of diffusion test results showed that the best concentration in inhibiting the growth of these two bacterias were the concentration of 25% and the sensitivity of Staphylococcus aureus from Laboratory pure cultivation and the Pus patients isolate of the Hospital toward extracts were the same.

Detection Detection Of Methicillin Resistant Staphylococcus aureus (MRSA) On The Patients Of RSUD Dr. Moewardi surakarta Using Culture Method And Polymerase Chain Reaction (PCR) Siti Nur Arsih; Nony Puspawati; Rizal Maarif Rukmana
Biomedika Vol 12 No 2 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

ABSTRACT Methicillin Resistant Staphylococcus aureus (MRSA) is type of Staphylococcus aureus resistant to beta-lactam antibiotics, such as meticillin, penicillin, amphycillin, and amoxycillin. The percentage of MRSA occurrence in Indonesia is quite high namely 23.5%. MRSA detection can be done using culture method and Polymerase Chain Reaction (PCR). This research aims to find out the comparison of speed and sensitivity between the culture method and Polymerase Chain Reaction (PCR) in detecting Methicillin Resistant Staphylococcus aureus towards the patients of RSUD Dr. Moewardi Surakarta. This research used experimental analytic research design along with comparative research design. This experiment observed 3 samples of bacterial isolates Staphylococcus aureus MRSA suspect patients of RSUD Dr. Moewardi Surakarta. The detection of MRSA was conducted using disk diffusion test of penicillin, amphycillin, amoxycillin and vancomisin as well as using Polymerase Chain Reaction (PCR). The results of the research indicate that Polymerase Chain Reaction (PCR) method was faster and more sensitive to detect Methicillin Resistant Staphylococcus aureus on the patients of RSUD Dr. Moewardi Surakarta.

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