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Jurnal Biomedika
Published by Universitas Setia Budi
ISSN : 20892136     EISSN : 23021306     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health
JURNAL BIOMEDIKA (p-ISSN:1979-035X, e-ISSN: 2302-1306) is a scientific publication media that accommodates the scientific creativity of lecturers and researchers as outlined in scientific writing, both for academics in Setia Budi University and observers of medical biology and health. Articles published are the results of research in the field or in laboratories, scientific studies and studies of books that have never been published in other media. Information on scientific works can range from biotechnology, health, pharmacology, microbiology, biochemistry, and food analysis and health management.
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Articles 199 Documents
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Analysis of Interleukin-17 Levels in Patients with Thrombocytopenia Lidwina Septie Christyawardani; Mansyur Arief; Uleng Bahrun
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.707

Abstract

Thrombocytopenia or platelet deficiency is a condition, in which platelet level in the blood circulation is below normal, which is less than 150,000 cells/µl. Thrombocytopenia is classified into some conditions, including decreased platelet production, increased need for platelets, and other thrombocytopenia. The need for increased platelets can be subdivided into primary immune thrombocytopenia, secondary immune thrombocytopenia, non-primary ITP, and thrombocytopenia that are not immune-mediated. Several cytokines play a role in the process of thrombocytopenia, one of which is Interleukin-17 (IL-17) that will be further discussed in this study. A previous study reported that IL-17 production increased in ITP and cITP patients. The objective of this study was to analyze the IL-17 levels and figure out the differences in IL-17 levels in the serums of patients with primary ITP and secondary ITP. The samples were taken from Wahidin Sudirohusodo Hospital and the specimens were examined in the Research Unit Laboratory of the Faculty of Medicine, Universitas Hasanuddin/Hospital of Universitas Hasanuddin. The comparative test resulted in p-value = 0.005, where p <α = 0.05; and therefore, there was a significant difference between IL 17 levels in ITP and non-primary ITP.
Analysis of Interleukin-4 in Thrombocytopenia Autoimmune Patients Sitti Nurfaizah; Mansyur Arief; Uleng Bahrun
Biomedika Vol 13 No 2 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i2.708

Abstract

Thrombocytopenia is a disease characterized by a decreased platelet count. Some of the causes are decreased platelet production, increased platelet use, such as due to infection, and autoimmune causes, namely the loss of tolerance of the immune system to self-antigens on the surface of the platelets and megakaryocytes marked with a platelet count <100,000 / μL and based on the pathomechanism classified as primary Immune Thrombocytopenia (ITP) and secondary, as well as several other causes. IL-4, one of the cytokines produced by Th2 which stimulates B cells to increase antibody production. The aim of this study was to compare IL-4 levels in primary ITP patients and non-primary ITP tombocytopenia. This study involved 30 primary ITP subjects and 30 non-ITP primary tombocytopenia subjects obtained based on data medical records, examination of IL-4 cytokine levels by the ELISA method. The results of this study that the IL-4 levels of the primary non ITP tombocytopenia subject group were higher than the primary ITP subject group, which means that there were differences in IL-4 levels in the primary ITP subject group and the non-ITP primary tombocytopenia subject group.
Detection of LytA Genes in Streptococcus pneumoniae Isolated from sputum pneumonia patients Mustika Sari Hutabarat; Firdaus Hamid; Irawaty Djaharuddin; Alfian Zainuddin; Rossana Agus; Muhammad Nassrum Massi
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.718

Abstract

Streptococcus pneumoniae (pneumococcus) is a Gram-positive facultative anaerobic bacterium that is a major cause of morbidity and mortality worldwide. But the lack of reporting of disease by this bacterium in Indonesia, one of the causes is because the diagnosis of pneumococcal infection is often clinically not typical and conventional methods which are still the standard gold method often give false-negative results. So the purpose of this study was to evaluate the performance of culture and molecular diagnostic methods using the Polymerase Chain Reaction (PCR) technique in detecting Streptococcus pneumoniae in sputum clinical samples using the Autolysin (LytA) gene which is a virulence factor of this bacterium. 57 isolates from 60 samples were confirmed as Streptococcus sp through microscopic identification, culture, and biochemical tests. Then the sensitivity test with an optochin test of 9 (9%) compared the results descriptively with the PCR technique using the Autolysin A (LytA) gene which was obtained more sensitive by 15 (25%).
The Effect of Seaweed Combination on the Extract of Robusta Coffee (Coffea robusta) Waste Extract in Producing Facial Mask Products Muhammad Ridwan Harahap; Nizar Mauliza; Anjar Purba Asmara; Elsa Citra Lestari; Winda Afriani
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.745

Abstract

Aceh is one of the biggest coffee producers in Indonesia’s province. The coffee farmers separate the coffee beans with the flesh of the fruit for processing the seeds, while the coffee fruit is considered as waste used as animal feed. Based on existing research, the coffee flesh produced contains polyphenol compounds as antibacterial. A very promising sea product from Aceh is seaweed. Brown seaweed (Sargassum sp.) is very common in the west coast of Aceh province. One of the derivatives is alginate. In this study, a combination of polyphenols extracts from coffee flesh and alginates from seaweed Sargassum sp. used as a basic ingredient in making face masks. Tests on organoleptic observations, pH testing, testing time of dried preparations, and testing of bacterial activity have been carried out in this study. In addition, variations in the mixture preparation based on the particle size of alginate powder have also been carried out in this study. The results obtained indicate that the yield produced from coffee fruit extracts is 5.86%, the highest yield of alginate is by potassium carbonate of 40.77%, the acidity of the combination is 4.5 - 6.5. For preparations, drying time has an average estimated time of approximately 6 minutes. Bacterial activity test showed that K2CO3 extracting agent concentration of 2% was strong against bacterial growth with a clean area of ​​14 mm. With these results, it can be seen the combination is able to inhibit the rate of bacterial growth, so it is recommended for alternative ingredients in the manufacture of cosmetics pharmaceutical industry masks.
The Profiles of Hepatitis B and C Virus Infections in Prison-Assisted Citizens Patricia Gita Naully
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.746

Abstract

Prison-assisted citizens or prisoners are one of the groups of people who are at high risk of Hepatitis B and C virus infections. The data on the prevalence of both viruses in prisoners in Indonesia are still limited. This study aims to determine the profiles of Hepatitis B and C virus infections in prison-assisted citizens in the Class IIA Narcotics Correctional Institution in Bandung Regency. A total of 30 prisoners were used as the samples in this study. All procedures performed in this study were following the applicable codes of ethics. The presence of surface antigens of Hepatitis B virus (HBsAg) in serums was detected using a qualitative sandwich Enzyme-Linked Immunosorbent Assay method. The existence of antibodies of the Hepatitis C virus (Anti-VHC) was detected using the immunochromatography method. The laboratory test results have shown five people (16.7%) were positive on the HBsAg test and one person (3.3%) was positive on the anti-VHC test. One case of Hepatitis B and C co-infection was also found in the prison-assisted citizens who were in the Class IIA Narcotics Correctional Institution in Bandung Regency. All the prisoners infected by the Hepatitis B and C viruses used injection drugs, had tattoos on their bodies and never received vaccinations.
Metagenomic Diversity of Gut Microbiota of Gestational Diabetes Mellitus of Pregnant Women Minarti Minarti; Nurhidayat Triananinsi; Nurqalbi Nurqalbi; Sumarni Sumarni; Mudyawati Kamaruddin
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.747

Abstract

Gestational Diabetes Mellitus (GDM) is defined as a condition in which a woman without diabetes develops abnormal glucose tolerance that is first recognized during pregnancy. GDM is a significant public health problem with an incidence of 1.9 – 3.6% of all pregnancies in Indonesia. Additionally, women with GDM during pregnancy have a high risk of developing diabetes when they are not pregnant, such as type 2 diabetes (T2D). One alternative variable in the management of T2D globally is gut microbiota. Here, to find out the role of gut microbiota in pregnancy, we characterized the stools of 30 pregnant women, each consisting of fifteen GDM-detected pregnant women, and healthy pregnant women using metagenomic approach with genome analysis by directly isolating genomic DNA from the microbiota ecosystem that occupies the digestive tract. DNA sequencing results were analyzed by MEGA 6 software with the BLASTn algorithm in NCBI. Thus fifteen GDM-detected showed high nucleotide sequence homology with the Proteobacteria at phylum level, and Escherichia, Orchobacterium, Cronobacter, Shigella, Salmonella, Enterobacter, Klebsiella, Kosakonia, Vibrio dan Gamma-Proteobacterium at genus level compared to the healthy pregnant women which found by Firmicutes at phylum level and Ruminococcus, Clostridium, Clostridiales, Lachnospiraceae, Roseburia, Weisella, Eubacterium at genus level had a higher abundance in healthy pregnant women. In this result, we found also one of the fifteen healthy pregnant women showed differential abundance with enrichment of Prevotella species. Gut microbiota of GDM-diagnosed pregnant women has more varied composition, and dominated by the phylum Proteobacteria than in normal pregnant women.
The Potential of Iodine Lactoglycerol as an Alternative Fungal Stain in the Laboratory Lilis Puspa Friliansari; Erick Khristian; Sofie Agustina Hermawanti
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.748

Abstract

Fungi are eukaryotic and opportunistic organisms that can cause damage and disease. Fungi can be identified by direct staining. The composition of the dyes used in fungi staining are toxic, mutagenic and harmful to laboratory personnel and the environment. Consequently, it is necessary to consider use fungi dye continuously, so its need alternative safety dyes to identify fungi in the laboratory. This research aims to determine the potential of iodine lactoglycerol with different concentrations (1.25%, 2.5%, and 5%) as an alternative dye for fungi in the laboratory. The research method used experimental design. The samples originate from moldy bread and made 27 of microscopic slides. The microscopic slides were observed with objective 40x magnification. The parameters assessed in this study were the structure and morphology of fungi including hyphae, conidiophores, vesicles, sterigmata and conidiospores and color intensity using the image analysis of ImageJ. The results showed that various concentrations of iodine lactoglycerol can staining the structure and morphology of the fungi properly and clearly. The value of color intensity of each concentration measured has a different average. The highest average value of color intensity concentration was 5% and the lowest was 1.25%. However, the result of ANOVA test showed a significance of 0.380 (P> 0.05), which means that is no difference significantly from three of various concentrations. The result showed that the difference concentration of iodine lactoglycerol does not affect the color intensity. The conclusion of this study was iodine lactoglycerol solution can be used as an alternative dye or Lactophenol-cotton Blue (LPCB) substitute for fungi identification. It is recommendation for further research be carried out regarding the decrease in the concentration of alternative color solutions below 1.25%, the incubation time for the preparation of the preparations, and the color stability of the prepared mushroom preparations.
The Effectiveness of Red Grape (Vitis vinifera) Seed and Skin Extract Treatment on Staphylococcus aureus Bacteria that Causes of Urinary Tract Infections in Pregnant Women Examined by using White Mice (Mus musculus) Satriya Manggala Liastra; Ninuk Dwi Ariningtyas; Yelvi Levani
Biomedika Vol 13 No 2 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i2.750

Abstract

Urinary tract infection or what is often called a UTI is an infectious disease of the urinary tract epithelium and is commonly caused by gram-positive bacteria, namely the Staphylococcus aureus, with a prevalence rate of 68 million patients in the world and 1.2 million patients in Indonesia. The diagnosis of UTI is established with a positive urine culture significance value ≥105 (CFU)/ml. The existing clinical management for cases of urinary tract infections in pregnant women uses amoxicillin to kill gram-positive bacteria, such as Staphylococcus aureus. However, there have been 469 cases of antibiotic resistance in pregnant women in Indonesia due to the non-compliance of using antibiotics for urinary tract infections. Vitis vinifera can be used as an alternative to natural bactericidal resveratrol containing anthocyanidin to kill gram-positive bacteria. This study used 28 white mice (Mus musculus) aged 6-21 weeks and weighing 120-200 grams injected with Staphylococcus aureus. The 28 subjects were divided into 4 groups, each of which consisted of 7 mice. The mice received K1 treatment with 250 mg/kgBW of amoxicillin and treatments with 200, 300, and 500 mg/KgBW red grape seed and skin extract. Before treatments were given, a pre-test was conducted to ensure that the results were not biased. Post-test was also performed after the Staphylococcus aureus injection, which was measured after 5 days of urine culture that reached 107 CFU/ml. The treatment in P3 using 500 mg/KgBW of red grape seed and skin extract contributed to the smallest result with 102 Cfu/ml, equals to the result produced by giving 250 ml/KgBW of amoxicillin.
The Effect Cytotoxic and Apoptotic Propagation Chloroform Fraction of Ocimum sanctum L. Towards Hela Cells Line Culture Ghani Nurfiana Fadma Sari; Sri Rejeki Handayani
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.774

Abstract

Materials used in the research are Ocimum sanctum L, a herbaceous shrub used in traditional medicine displaying as immunomodulator, anti-stress, hepatoprotective, kemopreventif, and anti-inflammatory. A research to identify its ethanol extract of Ocimum sanctum has been conducted, showing that it possesses cytotoxic activities to HeLa cells with IC50 value of 209µg/ml. The aim of this research is to find out activities cytotoxic and apoptotic propagation chloroform fraction of Ocimum sanctum towards HeLa cells line. The extraction was done using maceration using ethanol 96% solvent and fractionation was done using chloroform. Effects of chloroform fraction on HeLa cells were tested using MTT at various dosages (500, 250, 125, 62.5, and 31.25) µg/ml. Cisplatin (Kalbe) was applied as positive control. The effect apoptotic propagation chloroform fraction of Ocimum sanctum on HeLa cells was observed by DNA painting method using orange ethridium bromide-acridine. The test results showed that the chloroform fraction of Ocimum sanctum extract on HeLa cells had an IC50 value of 155.67 µg / ml ± 46.99 and Cisplatin had an IC50 value of 16.81 µg / ml ± 20.37. Double staining test shows the existence of cells undergoing apoptosis. Based on these results, the chloroform fraction Ocimum sanctum has been shown to be cytotoxic and has the possibility of stimulating apoptosis of cervical cancer cells (HeLa). This can be used as a basis for the development of these plants as anticancer agents with specific action targets.
Comparison of Pap Smear Preparation Quality Containing Orange G and Without Orange G on the Results of the Papanicoulou Painting Intan Prihatin; Budi Santosa; Fennisia Wibisono
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.775

Abstract

Examination procedure for pap smear preparation is influenced by fixation, drying and painting, and interpretation capability of examination. The Papanicolaou painting uses Harris Hematoxylin, Eosin Azure (EA) -50, and orange G. Orange G colors the orange matured and keratin cells Keratinizing-pink/orange cells. Chromatin in the nucleus will bind alkaline paint (hematoxylin) and cytoplasm protein will bind acidic paint (Orange G) and nucleus in the core will bind acid paint (EA 50) so that the cell will turn into pink with a blue core. The coloring stage according to procedure is using Orange G, but some PA laboratories do not use Orange G. The research goal is to know the comparison of pap smear preparation quality which contains orange G and without orange G towards papanicoulou painting result. The research type is experimental; the sample was 16 samples with two treatments namely coloring which using Orange G and without Orange G. The preparation quality which contains Orange G obtained moderate result of 12.5%, and good 87.5%. The preparation quality without Orange G was not good result of 6.3%, moderate 18.8%, and good 75%. The statistical test result of Chi Square and Kappa showed that the result was p>0.05 which means that comparison result of preparation quality which contains orange G and without orange G there was no significant difference.

Page 17 of 20 | Total Record : 199

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