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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
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Analysis of Hematologic Parameters and Serum Bilirubin Levels in Complicated and Uncomplicated Acute Appendicitis Patients Rini Rahman; Ani Kartini; Yuyun Widaningsih; Agus Alim Abdullah
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1536

Abstract

  Acute appendicitis is inflammation of the vermiformis appendix due to lumen obstruction followed by bacterial infection. Acute appendicitis is classified into two types of disease, namely complicated and uncomplicated. Acute inflammation of the appendix requires immediate surgical action to prevent complications; therefore, a further laboratory test is required. This study was retrospective research which analyzed several hematologic parameters (WBC, Neutrophils, NLR, RDW, PLTMPV, PDW) and serum bilirubin levels. Data were statistically analyzed using Independent-t and Mann-Whitney test. Univariate logistic regression test was used to evaluate the correlation of hematologic parameters and significant serum bilirubin levels with the diagnosis of complicated and uncomplicated appendicitis. The cut-off value and diagnosis value were measured using Receiver Operating Characteristic (ROC) curve analysis. This study involved 173 subjects categorized into 67 complicated and 106 uncomplicated acute appendicitis patients. Whole blood count and neutrophil counts were significantly higher in complicated acute appendicitis (p <0.05). Similarly, NLR and PLT were significantly higher in complicated acute appendicitis (p <0.001), p > 0.05 was obtained in RDW-CV, MPV and PDW values. The higher serum bilirubin levels were reported in patients with complicated acute appendicitis compared to uncomplicated acute appendicitis (p <0.001). Logistic regression test results showed significant PLT parameters with p <0.001, while there was no significance of hematologic parameters such as WBC, neutrophil, NLR, RDW-CV, MPV, PDW and bilirubin levels. Area under curve results on the ROC curve showed 74.4% PLT with sensitivity and specificity of 97.17% and 40.29%, respectively. From the analysis of hematologic parameters and measurement of serum bilirubin levels, only the PLT parameter can be used as one of the parameters for the diagnosis of complicated appendicitis with high sensitivity but low specificity. It was recommended to perform prospective studies with more subjects.
SIMVASTATIN GENERIK DAP. Rasmika Dewi; DG. Diah Dharma Santhi; DM Sukrama; AA. Raka Karsana
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1076

Abstract

This study aims to know and determine the lipid profile in patients with hyperlipidemia who consumed Generic Simvastatin comparedwith its patent product contained in the Formularium at Sanglah Hospital. The observations made, were the measurement of the totalcholesterol and low-density lipoprotein (LDL) before and after the drug administration. A total of 30 subjects who met the inclusioncriteria, were divided into two (2) groups, each group consist of 15 persons, the first group was given 20 mg generic Simvastatin(1 tablet daily) for 15 days and Group II given 20 mg patent Simvastatin (1 tablet daily) for 15 day. After 15 days, their blood sampleswere taken and examined for total cholesterol and LDL. Once the data were collected, statistical analysis was done by using the normalitytest, homogeneity and t. Statistical analysis using p-value less than or equal to 0.05 was the limit of significance. The statistical analysisshowed that the data was normally distributed and homogeneous (p≥0.05). The T-test showed that there were significant differencesin the levels of total cholesterol and LDL serum samples before and after the administration of generic simvastatin and patents the(sig.=0.000). However, there was no significant difference in decreased levels of totall cholesterol samples between the generic Simvastatinand patent (sig=0.365 with α=0.05 level). Besides this, there was also no significant difference in the decreased levels of LDL betweengeneric Simvastatin and the patent one (sig=0.372 with α=0.05 level).
CORRELATION OF TOTAL LYMPHOCYTE COUNT WITH CD4 COUNT IN HIV/TB COINFECTED PATIENTS Herniaty Rampo; Uleng Bahrun; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1393

Abstract

HIV-TB co-infection is a global challenge in the healthcare world. HIV infection causes CD4  to decrease which has a role in TB immunity. CD4 as a marker for HIV progression have expensive cost and not available in all healthcare facilities, so WHO recommended TLC as a substitute for CD4. This study aims to determine the correlation of TLC and CD4 in HIV-TB co-infection patients.This was a retrospective study using medical records of hospitalized patients with HIV-TB co-infection in Dr.Wahidin Sudirohusodo Hospital. The correlation was analyzed by Spearman test. CD4 data were divided into two groups: CD4 < 200 cells/mm3 and CD4 ≥ 200 cells/mm3 and Mann-Whitney test was performed. Data were also analyzed by ROC curve.In total 172 data with diagnosis of HIV-TB co-infection, Spearman test showed a positive correlation between TLC and CD4  with moderate correlation strength (p <0.001 and r = 0.56). Mann-Whitney test showed  a significant difference between CD4 < 200 cells/mm3 and CD4 ≥ 200 cells/mm3 (p < 0.001). ROC curve analysis showed that TLC has a good accuracy value for predicting CD4 in CD4 < 200 cells/mm3 (AUC = 0.911). Sensitivity  88.9% with specificity 84.7% was found in TLC 1154.9 cells/mm3 which predicted CD4  < 200 cells/mm3.There was a positive correlation between TLC and CD4 with moderate correlation strength. TLC can predict CD4 in CD4 <200 cells/mm3. We suggest further studies on patients who receive drug therapy.
CORRELATION BETWEEN VISCERAL ADIPOSE TISSUE-DERIVED SERPIN WITH FASTING BLOOD GLUCOSE LEVEL IN OBESITY Novi Khila Firani; Agustin Iskandar; Anik Widijanti; Nonong Eriani
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 1 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i1.1150

Abstract

Abnormalitas jaringan lemak pada kegemukan berhubungan dengan timbulnya berbagai masalah kesehatan, antara lain terjadinyaresistensi insulin. Adipositokin merupakan protein yang dihasilkan jaringan lemak, salah satunya adalah Visceral Adipose Tissue-DerivedSerpin (Vaspin). Beberapa penelitian menunjukkan vaspin berhubungan dengan kepekaan insulin. Belum diketahui apakah dalam setiappeningkatan derajat kegemukan terdapat perubahan hasilan vaspin, yang berhubungan dengan perubahan kadar glukosa darah, sebagaimanifestasi gangguan kepekaan insulin. Rancangan penelitian adalah potong silang, dengan jumlah sampel 60 orang usia dewasa,yang terbagi berdasarkan patokan WPRO (2000), yaitu 10 orang non-kegemukan, 10 orang kegemukan I dan 40 orang tergolongkegemukan II. Pemeriksaan kadar glukosa darah puasa menggunakan metode heksokinase. Pemeriksaan kadar vaspin menggunakanmetode sandwich ELISA. Telitian menunjukkan kadar vaspin di kelompok kegemukan II dan I lebih tinggi dibandingkan kadar vaspinpada non-kegemukan (p=0,00). Kadar vaspin di kegemukan II dan I tidak berbeda bermakna. Kadar glukosa darah puasa di kelompokkegemukan II dan I lebih tinggi dibandingkan kadar glukosa darah puasa di non-kegemukan (p=0,017), namun kadar glukosa darahsebagian besar subjek penelitian masih dalam taraf normal. Hasil uji kenasaban Spearman menunjukkan ada kenasaban positif yangbermakna kadar vaspin terhadap kadar glukosa darah, namun kekuatannya rendah (r=0,384, p=0,001). Terdapat hubungan yangbermakna kadar vaspin dengan kadar glukosa darah puasa di kegemukan. Perlu dilakukan kajian lebih lanjut menggunakan subjekpenelitian kegemukan dengan mengukur indeks kepekaan insulin, untuk memperjelas hubungan antara vaspin, sebagai adipositokinyang berperan dalam kepekaan insulin, terhadap kadar glukosa darah.
HUBUNGAN DERAJAT PERLEMAKAN HATI NON-ALKOHOLIK DENGAN AKTIVITAS AMINOTRANSFERASE SERUM Nyoman Trisna Yustiani; Mutmainnah .; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.1027

Abstract

Fatty liver could be related to alcoholic or non-alcoholic. Mild to moderate elevation of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities are the most laboratories test ordered in patient with non-alcoholic fatty liver. This studyhas purposed to know the relation of degree non-alcoholic fatty liver’s with aminotransferase serum activity. A cross-sectional studywas done in 38 patients with non-alcoholic fatty liver aged 30 to 60 years at Clinical Pathology Laboratory Installation and RadiologyDepartment Dr. Wahidin Sudirohusodo Hospital in Makassar from March to July, 2008. Patient made to criteria done some laboratorytest for AST and ALT examination. Data was analyzed using T-Test in SPSS for windows version 11,5. In this study we found that therewere a significant difference between non-alcoholic fatty liver degree with AST and ALT activity in cutt off 18 (p < 0,05). From this studywe could conclude that the activity of AST and ALT serum can be used to predict probability of non-alcoholic fatty liver.
DAN MYELOPEROxIDASE) DAN DISFUNGSI ENDOTEL (ASIMETRIK DIMETILARGININ) DI KEGEMUKAN (OBESITAS) Joko Widodo; Burhanuddin Bahar; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 3 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i3.1039

Abstract

Obesity is a pathological condition in which there is an excess body fat due to imbalance energy expenditure. Its association with oxidative stress could cause other metabolic disorders such as endothelial dysfunction, atherosclerosis, and cardiovascular disease. Theaim of this study was to assess the correlation of oxidative stress (F2-Isoprostane, Superoxide dismutase and Myeloperoxidase) andendothelial dysfunction (Asymmetric dimethylarginine) which happened in central obese men. A cross sectional study was carried outin 62 central obesity male subjects with ages range between 30−60 years. The researcher determined SOD activity, concentration ofMPO as well as ADMA. In this study was found a significant correlation of F2-Isoprostan (r = 0.333, p = 0.008), MPO (r = 0.386; p = 0.008) and ADMA but not with SOD. The elevated concentration of F2-Isoprostane occur 3.5 times (p = 0.02; 95%; CI = 1.19–10.19), elevated MPO occur 3.7 times (p = 0.023; 95%; CI = 1.16–11.56) while combination of elevated F2-Isoprostane-MPO occur6.7 times (p = 0.011; 95%; CI = 1.33-33.24) will increase the risk of endothelial dysfunction. There was a significant correlation of oxidative stress with endothelial dysfunction, and the increase concentration of F2-Isoprostane and MPO indicates the occurrence of endothelial dysfunction in central obesity.
PREDIKSI JUMLAH SEL LIMFOSIT T CD4+ MENGGUNAKAN NILAI TLC (TOTAL LYMPHOCYTE COUNT) PADA PENDERITA HIV/AIDS Rostina Rostina; Suci Aprianti; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 2 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i2.902

Abstract

AIDS is a severe disease caused by Human Immunodeficiency Virus (HIV) that affects patient’s immune system, especially CD4+ Tcells (CD4). Hence, CD4 count is used as parameter to starting ARV treatment or monitoring the progress of the disease. However, themeasurement of CD4 is expensive and available in big hospitals. In small or remote hospitals there are no means to measure the CD4.Some studies suggest that in an area where CD4 count is unavailable, the total lymphocyte count (TLC) of HIV/AIDS patients can roughlybe used to predict CD4 values. This study is aimed to see whether the TLC values can be used to roughly predict the CD4 count of HIV/AIDS patients and to formulate the correlation form between them. A cross sectional study design was applied to 79 blood samples ofHIV/AIDS patients from Clinical Pathology Laboratory of Wahidin Sudirohusodo Hospital from January to September 2007. The bloodsamples were tested for TLC as well as CD4 values. The correlation of TLC and CD4 values was tested with Pearson Correlation Test andthe correlation formula was derived from curve estimation of Regression Analysis. Sensitivity, specificity, PPV and NPV of various cutpoint of TLC (1000, 1200, 1500, 2000) to predict CD4 < 200/ul were determined using cross tabulation Fisher Exact Test. A positivecorrelation was found between TLC and CD4 count (R = 0.528, p < 0.001) with the regression formula is CD4 = 0.09TLC – 1.42.The WHO standard cut point TLC1200/ul give best result for sensitivity, specificity, PPV and NPV: 80.6%, 91.7%, 98.2% and 45.8%,respectively. The cut point of TLC1200 can be used to roughly predict CD4 < 200/ul of HIV/AIDS patients, so, can be use as a mark forstarting ARV therapy in the place were measurement of CD4 is unavailable
RDW, JUMLAH TROMBOSIT DAN RPR DENGAN INDEKS FIB-4 DI HEPATITIS C Yenny Yulianti; Banundari Rachmawati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i2.1118

Abstract

Hepatitis C virus infection is one of the main causes of worldwide chronic liver disease. The determining of fibrosis level in the liverdisease is essential. The red blood cell distribution width (RDW) is a potential prognostic index for liver disease. The platelet (PLT)count has been used as the biomarker for liver fibrosis. RDW to platelet ratio (RPR) is devised to amplify the difference in the RDW andplatelets among patients with different liver fibrosis stages. Fibrosis 4 (FIB-4) indexes are accurate non-invasive methods to predict thelevel of liver fibrosis of HCV-monoinfected patients. The objective of this study is to know the correlation of RDW, PLT count, and RPR withFIB-4 index in hepatitis C patients by analyzing them. The study was carried out observationally with cross sectional approach betweenFebruary−March 2015 at the Dr. Kariadi Hospital, on samples collected consecutively from the medical records of hepatitis C patients.The data processing was performed with Pearson/Spearman correlation. There was a strong positive correlation between RDW and FIB-4index (r=0.624; p=0.000) and between RPR with FIB-4 index (r=0.674; p=0.000), while there was a strong negative correlationbetween PLT count and FIB-4 index (r=-0.600; p=0.000). From this study it can be concluded that there was an increased RDW and RPRresulting in a higher FIB-4 index. There was also found a decreased PLT resulting from higher FIB-4 index .The opinion of the researchersis that further studies for prospective multicentres are needed to be carried out, so that the results can be more generalized.
AKURASI TES BACTIDENT AMINOPEPTIDA SE UNTUK MENGIDENTIFIKASI BAKTERI GRAM NEGATIF Ramla Tongko; Tenri Esa; Hardjoeno .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.990

Abstract

The information about Gram stain of a bactery can help the clinicians to choose good antimicrobial therapy because many of antimicrobial drugs have activity to positive or negative Gram bacterial selective. So it needed to have a method to early identificationGram stain of bactery, which can be faster, cheaper and more practice. Identification of Gram stain we can do with BactidentBactident aminopeptidase test, Gram stain test and culture test. The aimed of this study is to know the accuracy of bactident aminopeptidase test, Gram stain test and culture test. The aimed of this study is to know the accuracy of bactident aminopeptidase test with culture test in identify Gram negative bactery. A diagnostic test was done among 60 samples (pus and sputum) at Sub UnitInfection Disease Dr.Wahidin Sudirohusodo public Hospital of Makasar from March to July 2008. Bactident aminopeptidase test, Gramstain test and culture test were done to each sample. The data was analysed with 2 × 2 table. Accuracy of Bactident aminopeptidaseBactident aminopeptidase test on culture test were high sensitivity 61.11%, specificity 100.00%, positive predivtive value (PPV) 100.00% and negative predictivevalue (NPV) 63.16%. Sensitivity of Bactident aminopeptidase test on culture test were lower than sensitivity of Gram stain test onBactident aminopeptidase test on culture test were lower than sensitivity of Gram stain test on test on culture test were lower than sensitivity of Gram stain test on culture test (61.11% vs 83.33%). Specificity of Bactident aminopeptidase test on culture test were higher than specificity of Gram stainBactident aminopeptidase test on culture test were higher than specificity of Gram stain test on culture test were higher than specificity of Gram stain test on culture test (100.00% vs 79.19%). Accuracy of Bactident aminopeptidase test on Gram stain and culture test is high enough,Bactident aminopeptidase test on Gram stain and culture test is high enough, test on Gram stain and culture test is high enough, it can lead us conclude that Bactident aminopeptidase test can be usefull to the clinician for using the antimicrobial before culture testBactident aminopeptidase test can be usefull to the clinician for using the antimicrobial before culture test test can be usefull to the clinician for using the antimicrobial before culture test was provided.
PENGENDALIAN MUTU BIDANG MIKROBIOLOGI KLINIK Prihatini Prihatini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 2 (2005)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i2.851

Abstract

The quality control program in microbiology are procedures to identify microorganism,monitoring,to asses laboratory competencein handling clinical materials, other methods needed.QC is essential in bacteriology because nature, unlike clinical chemistry andhematology QC’s, because can’t compare with control value. The QC in microbiology include preparation of pra-analitics,analitics andpost-analitics which depending to personal, material,SOP and micoorganism Specimens, laboratory Instruments like incubators, freezer,autoclaves, must be good preparations. Examinations use control standard, monitoring all of themes periodically The laboratory resultswere recorded and evaluated as reference laboratory. The QC microbiology have been presented, they need large budgets to validity oflaboratory results.

Page 51 of 133 | Total Record : 1328

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