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Jaringan Laboratorium Medis
Published by Poltekkes Kemenkes Semarang
ISSN : -     EISSN : 26858495     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Public Health
Jurnal Online Jaringan Laboratorium Medis adalah peer-reviewed journal yang mempublikasikan artikel-artikel ilmiah tentang ilmu kesehatan yang meliputi bidang Analis Kesehatan yang meliputi Penelitian Bakteriologi, Kimia Klinik, Parasitologi, Toksikologi Klinik, Hematologi, Sitohistoteknologi dan Imunologi, Teknologi Bank Darah. Dengan e-ISSN: 2685-8495 diterbitkan oleh Jurusan Analis Kesehatan Politeknik Kesehatan Kemenkes Semarang, terbit 6 bulan sekali, pada bulan Mei dan November setiap tahunnya.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 268 Documents
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Gambaran Stabilitas Pooled plasma CPDA Sebagai Bahan Kontrol Kualitas Pemeriksaan Asam Urat Pada Uji Presisi Within run dan Between day Setyaji, Yoki; Duri, Iin Desmiany; Setyowatiningsih, Lilik
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.10933

Abstract

The quality of laboratory test results is ensured by implementing quality control measures, which usually require control materials. The high cost of commercial control materials poses a challenge for laboratory quality control. Commonly, laboratory control materials are commercial control serums derived from animal serum that undergoes lyophilization during manufacturing. This study aims to assess the stability of CPDA pooled plasma that has been frozen for 60 days, using precision tests both within-run and between-day. Methods: This experimental research utilized CPDA pooled plasma samples from hematology laboratory waste at Poltekkes Kemenkes Semarang. The samples were frozen at -20℃ for 60 days. Quality control (QC) analysis was conducted using within-run and between-day precision tests without replication, to determine the standard deviation (SD) and coefficient of variation (CV). The results were then statistically analyzed using a one-way ANOVA to assess the difference in CPDA pooled plasma stability between the within-run and between-day precision tests. The QC results for within-day and between-day precision were as follows: the average for within-day was 4.066 and for between-day 3.2785; SD for within-day was 0.20 and for between-day 0.09; CV for within-day was 4.9% and for between-day 2.9%. The one-way ANOVA test showed a significance value of 0.316, indicating no significant difference between the within-day and between-day QC methods in terms of precision testing. Conclusion: CPDA pooled plasma remains stable in maintaining the precision of QC results for uric acid using the enzymatic colorimetric method (end-point) after 60-80 days of storage and can be used as an alternative to commercial control materials in uric acid QC testing.
Hubungan Antara Kadar HbA1c dengan Kadar Trigliserida terhadap Pasien Prolanis Diabetes Melitus Tipe 2 di Laboratorium Klinik Kimia Farma Depok Putri, Riantika Lutfiana; Aryani, Desi; Safari, Wulan Fitriani
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.12189

Abstract

Diabetes Mellitus (DM) is a group of metabolic disorders characterized by elevated blood glucose levels resulting from abnormalities in insulin secretion, insulin action, or both. Patients with Type 2 DM are at a higher risk of developing cardiovascular diseases, often indicated by dyslipidemia, which is characterized by elevated triglyceride levels, increased Low-Density Lipoprotein (LDL) levels, and decreased High-Density Lipoprotein (HDL) levels. This study employs an analytical observational design. The data obtained shows that controlled and uncontrolled HbA1c levels with normal triglyceride levels were found in 24.7% of the patients, while 75.3% had high triglyceride levels. The data were further analyzed using the Statistical Package for the Social Sciences 25 (SPSS 25) with a non-parametric test, specifically the Chi-Square continuity correction test, with a significance level of p 0.05. The Chi-Square test results indicated that H0 was rejected and Ha was accepted, with a p-value of 0.036 for the relationship between HbA1c and triglycerides. These results suggest a correlation between HbA1c levels and triglyceride levels in Prolanis Type 2 DM patients at Kimia Farma Clinical Laboratory in Depok. These findings can serve as an evaluation material for Prolanis DM Type 2 patients to control their glycemic levels by routinely monitoring their HbA1c and triglyceride levels.
Derajat Aglutinasi pada Pemeriksaan Golongan Darah Metode Tabung Berdasarkan Masa Simpan Test Sel A dan Test Sel B Hari Ke-0, Ke-2, Ke-4, Ke-6 dan Ke-8 Zatalini, Karinta Syifa; Kuncara, Rachmad Bayu; Sugihantono, Anung
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.11733

Abstract

Blood group examination is an examination that aims to determine the type of blood group. Cell test is a blood group examination reagent used to detect antibodies in the serum being examined. The long shelf life of cell tests that can only last for two days is considered less effective for agencies with a high level of blood services. The purpose of this study was to describe the degree of agglutination in blood group examination with cell test A and B stored on the 0th, 2nd, 4th, 6th, and 8th days. This study is a descriptive study with a Quasi Experimental research design. Test cell A and test cell B were made from red blood cell specimens of 3 blood type A and 3 blood type B respectively. Test cells are stored in a refrigerator with a temperature of 2-6° C. Test cells were then examined on the 0th, 2nd, 4th, and 6th day of storage. Calculation of samples and repetitions using the Federer formula with the number of treatments in this study is 5 treatments. Based on the calculation, one sample of test cell A and test cell B was obtained with five repetitions of each examination. The results showed that on the 0th, 2nd, 4th, and 6th day of cell test storage, the results of blood type examination were obtained, namely the degree of agglutination 4+ with erythrocytes in the cell test clumping into one bond, cells forming large agglutination with clear supernatant. On the 8th day of storage, the result of agglutination degree is 3+ with erythrocytes in test cells not clumping perfectly, there are erythrocyte granules and cloudy supernatant. Based on the results of the study, it is concluded that test cell A and test cell B can be used optimally until day 6 storage.
Faktor Risiko Terjadinya Anemia pada Pasien Tuberculosis Paru dalam Fase Pengobatan Rosidah, Umi; Auliya, Qurrotu Ayuni; Wikandari, Ririh Jatmi; Widiyanto, Santo Yoseph Didik
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.12268

Abstract

Tuberculosis lungs is disease infectious disease caused by bacteria Mycobacterium tuberculosis. Tuberculosis is still a disease become threat health Indonesian society which  leads to illness , disability and death tall so that need existence mitigation. Research objectives know factor risk the occurrence of anemia in patients tuberculosis lungs in phase treatment . Study This is study retrospective analytic case control Study carried out at the Community Health Center Very Beautiful Subdistrict Genuk City of Semarang. Population all TB patients who are undergo treatment at the Community Health Center Bangetayu. Research sample This from the total population namely 34 case and control patients. The results of 34 respondents case control there is level hemoglobin in patients Tuberculosis lungs with anemia (case) as many as 17 respondents experienced decline hemoglobin levels, namely man with an average hemoglobin level of 12.2 gr/dL (7 respondents), women with The average hemoglobin level was 10.6 gr/dL (2 respondents). A total of 17 respondents non - anemic control , namely the majority is normal with type sex Woman with an average Hemoglobin level of 12.61 gr/dL (17 respondents ). Based on characteristics what kind sex male 29.41% and gender sex women 11.76%. Decrease hemoglobin levels , namely man with an average hemoglobin level of 12.2 gr/dL (5 respondents), women with average hemoglobin level 10.6 gr/dL (2 respondents). Based on characteristics of the type sex women 70.59% (24 respondents) and men 29.41% (10 respondents). Based on characteristics age category adults 64.71% (22 respondents), elderly 23.53% (8 respondents), infants and children 11.76% (4 respondents), and adolescents 0.00% (0 respondents). The results of the bivariate test of hemoglobin levels, age , and type sex there is connection between hemoglobin levels, age, and type sex to Pulmonary Tuberculosis patients in phase treatment. The most influential risk factor o to the occurrence of anemia in patients with pulmonary tuberculosis in phase treatment namely on the variables type sex that is own risk of 3,429. Next followed by variables hemoglobin levels that have risk of 0.370. Age No there is factor risk the occurrence anemia in pulmonary tuberculosis patients in phase treatment. The conclusion of this study is that hemoglobin levels and sex are risk factors for anemia in patients with pulmonary tuberculosis in the treatment phase. Anemia can affect the diagnosis, prognosis, complications, and response to tuberculosis therapy, so that tuberculosis patients can maintain nutritional intake and therapy compliance to suppress the progression of the disease.
Hubungan Kejadian Hipertensi dengan Kadar Trigliserida pada Supir Bus di Terminal Arjosari Kota Malang Diki, Asrini Rambu; Fatmawati, Diana Noor; Husnah, Yeni Avidhatul
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.12140

Abstract

Hypertension is a health problem that is widely encountered in the community and correlates with other diseases. There are many factors that cause hypertension, one of which is abnormal triglyceride levels or hypertriglycerides. Higher levels of triglycerides will inhibit the occurrence of lipogenesis. Lipogenesis is a factor that causes hypertension from food intake, this is because food has a significant role in increasing blood pressure, especially protein and fat. This study aims to determine the relationship between triglyceride levels and hypertension. This type of research is an analytical research with the crosssectional method, statistical data analysis using correlation regression, the number of population as many as 50 respondents and the sample used as many as 21 respondents. Data collection using questionnaires, blood pressure measurements and triglyceride level checks. Blood pressure measurement is done manually and then for the examination of triglyceride levels using the GPO-PAP method is carried out in the laboratory of the Bareng Health Center using a spectrophotometer. The statistical analysis used is the correlation Pearson test. The results of the study on bus drivers with high blood pressure showed that some respondents had high triglyceride levels because they often ate fatty foods. Based on the examination that has been carried out on 21 bus driver respondents at the Arjosari Terminal, Malang City, it shows that triglyceride levels and high blood pressure have no relationship, with the test results using the Pearson correlation statistical test obtained a result of P=0.0766 0.05 with a Pearson value of P=-0.069 correlation meaning that it does not have a relationship with the degree of very weak relationship.
Gambaran Kadar Ureum, Asam Urat, Kreatinin, dan Albumin Pasien TB-MDR yang Mengkonsumsi OAT di RSP Dr. Ario Wirawan Salatiga Qomariyah, Nurul; Hidayah, Nur; Rahmaddina, Selma Aulia
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.12348

Abstract

Multi Drug Resistant Tuberculosis (MDR-TB) is a type of tuberculosis that is resistant to two first-line antituberculosis drugs (OAT), namely Isoniazid and Rifampicin. MDR-TB treatment involves a combination of first-line and second-line OAT, which is carried out over a long period of time. The prolonged use of these drugs can cause side effects that affect various organs of the body, especially the kidneys. One indication of impaired kidney function is an increase in blood levels of ureum, uric acid, creatinine and albumin. In addition, side effects of drugs such as Kanamycin, which is nephrotoxic, can cause accumulation in the proximal tubules of the kidneys, reduce the glomerular filtration rate, and impact creatinine levels. Another side effect that can arise during MDR-TB treatment is a decrease in appetite, which leads to decreased nutritional intake, reflected in low albumin levels. This study aims to describe the levels of ureum, uric acid, creatinine, and albumin in patients with MDR-TB undergoing treatment at Ario Wirawan Lung Hospital (RSPAW) Salatiga. This study is descriptive qualitative with a cross-sectional approach. Secondary data were collected from the medical record installation from January to December 2023. This study involved 28 respondents, of which 92.86% had normal ureum levels, 71.43% had normal uric acid levels, 96.43% had normal creatinine levels, and 78.57% had normal albumin levels. A small proportion of respondents had elevated ureum or uric acid levels, with some variation in the combination of abnormal creatinine and albumin levels. The conclusion of this study is that although there were patients with abnormal ureum, uric acid, creatinine, or albumin levels, MDR-TB treatment generally did not significantly affect ureum, uric acid, creatinine, and albumin levels in the majority of patients. However, regular monitoring of renal function and nutritional status is still required to detect and manage adverse effects that may arise during long-term treatment.
Uji Kualitas Telur Cacing STH Menggunakan Pewarna Alami Perasan Daun Jati (Tectona grandis) dengan Konsentrasi 40%, 60%, 80%, 100% sebagai Pewarna Alternatif Metode Sedimentasi Anggraini, Maysita Ari; Afrianti, Dina
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.11625

Abstract

Helminthiasis is a parasitic infection in the form of worm larvae or eggs that develop in the human body. Diagnosing helminthiasis with fecal examination uses 2% eosin dye to clarify and emphasize the shape of worm eggs. Eosin 2% is a class-3 IARC carcinogenic reagent, so a natural reagent that does not damage the environment is needed. Reagents from natural materials that can be utilized are teak leaves containing anthocyanin compounds that can clarify and emphasize the shape of worm eggs.  The purpose of this study was to determine the quality of sth (soil transmitted helminth) worm eggs using natural dye teak leaf juice (tectona grandis) with a concentration of 40%, 60%, 80%, 100% as an alternative dye sedimentation method. The design of this study is quantitative with experimental design with posttest with control group design with Kruskal-Walis test. The results of this study obtained the concentration with the highest average value, namely 40% concentration of 8.7. The higher the concentration, the less clear the morphology of the egg because of the influence of dirt / residue from the juice. The results of the Kruskal-Walis test sig value 0.054, it can be concluded that there is no difference between teak leaf juice with a concentration of 40%, 60%, 80%, and 100% with 2% eosin dye as a control. Based on the mean rank value of the Kruskal-Walis test, it can be seen that the best teak leaf juice concentration is 40% concentration with a value of 34.50. This research can be used as a reference for the utilization of teak leaves as a natural dye to replace 2% eosin for STH worm eggs.
Perbandingan Berbagai Metode Pengamatan Mycobacterium Tuberculosis secara Mikroskopis Widodo, Widodo; Sugiyatmini, Tri; Purlinda, Devi Etevia
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.12137

Abstract

Technological developments are increasingly rapid in the field of identification and special equipment in the field of microbiology, the development of staining methods and microscopic observation is growing rapidly. The Ziehl Neelsen staining method is still an alternative follow-up to other alternative treatments using the Auramin O staining method with flurocene microscope observation. This research is an analytical observational study and a cross sectional research design to see the differences in readings between Ziehl Neelsen staining, flurocene auramin and gram staining. We used isolates that had been identified as Mycobacterium tuberculosis which we obtained from Balepkes and Pak provinces of Central Java under non-activation conditions, by autoclaving at 121 degrees for 15 minutes. The number of samples was 10 slides with Ziehl Neelsen staining, Flurocene Auramin and Gram staining. The results of the sensitivity and specificity research between Ziehl Neelsen and auramin O were 100%. Meanwhile, the gram staining method and SEM observation cannot be compared because they can only observe bacterial morphology. From the research results it can be concluded that Auramin O can be used as an alternative Ziehl Neelsen painting agent.
Pemeriksaan Dampak Mengkonsumsi Minuman Beralkohol Terhadap Kadar Enzim Aspartat Aminotransferase (AST) dan Alanine Aminotransferase (ALT) Fatmawati, Shinta; Husnah, Yeni Vidhatul; Rahmawati, Previta Zeizar
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.11777

Abstract

Aspartat aminotransferase (ast) and alanine aminotransferase (Alt) or which is the enzyme transaminase used liver function tests. When alcohol enters the body, a metabolism in the liver is carried out with an end result of acetydehid when an excessive dose causes liver damage. The purpose of research to determine whether or not there is a correlation between the consumption of alcohol and its blast of bank. this study is a non-experiment of quantitative experimentation with a sampling technique. The respondents in this study are 24 alcohol drinkers of NTT students in the unfortunate city. And sample analysis methods of the kinetic ifcc and the chemical analyzer clinic - the mindray analyzer. On 24 of the respondents of all sexs-men will be analyzed based on long consumption, high consumption of alcohol, and heavy drink-alcohol consumption. The research data obtained will be done in a statistical test using the Pearson correlation test. Studies conducted on alcohol drinkers for a normal ast result are 18 respondents and abnormal results of 6. Studies on alcohol drinkers for normal test levels included 19 individuals and abnormal results for 5. The Pearson correlation test between the amount of consumption between the ast consumption is 0,184 and p-value 0.05, and the high consumption of alcohol is 0.508 and the value of p-value 0.05, and the amount of drinking is an e-0.405 and p-value 0.05. Studies between consumption length and Alt levels have found a very weak link of 0,033 and p-value 0.05, the high consumption of alcohol is 0,426 and the value of p-value 0.05, and the high drinking relationship of weak value are = 0.315 and p-value 0.05. The study has had a negative impact on excessive consumption of alcohol, raising levels of ast and Alt especially on high-alcohol drinkers and excessive drinking frequency.
Identifikasi Glukosa Urin dan Keton Urin pada Pasien Tuberkulosis Paru yang Mendapat Terapi Obat Anti Tuberkulosis (OAT) Di Puskesmas Tlogosari Wetan Hafidsya, Pradiana Syahwa; Kahar, Fitriani
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.12199

Abstract

Tuberculosis is a contagious infection caused by Mycobacterium tuberculosis. Anti-Tuberculosis Drug Therapy (OAT) given also has side effects, one of which is nephrotoxic. Laboratory examination of urine glucose identification can indicate if there is damage to the kidney glomerulus in the filtration process. The purpose of this study was to identify urine glucose and urine ketones in non-DM pulmonary TB patients with OAT treatment at the Tlogosari Wetan Health Center. This type of research is descriptive observational with a cross-sectional approach. The population was all TB patients who underwent examination at the Tlogosari Wetan Health Center. The research sample was a random urine sample from non-DM pulmonary TB patients. The examination method used was a dipstick using a reagent strip test. The results showed that out of a total of 30 non-DM pulmonary TB patients with OAT therapy (100%). A total of 6 (20%) patients were identified with urine glucose, with details of  intensive phase treatment 2 (7%) patients with trace urine glucose (±), while in the continuation phase 2 (7%) patients were identified with trace urine glucose (±) and 2 (7%) patients with low urine glucose (+1). The majority of positive urine glucose results were experienced by males with an elderly age range (46-65 years) of 4 patients (14%). The results of urine ketone identification showed that 2 (6.6%) patients were identified with urine ketones, namely trace (±) in intensive phase treatment, while 15 patients in the continuation phase had all negative urine ketones. The majority of positive urine ketone results were experienced by males with an adult age range (26-45 years) of 2 patients (14%). The conclusion of 30 non-DM pulmonary TB patients with OAT therapy who met the inclusion criteria, there were 6 (20%) patients identified with urine glucose and 2 (6.6%) patients identified with urine ketones. The impact of this study is expected for non-DM pulmonary TB patients to take OAT and have regular medical check-up.

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