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Jaringan Laboratorium Medis
Published by Poltekkes Kemenkes Semarang
ISSN : -     EISSN : 26858495     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Public Health
Jurnal Online Jaringan Laboratorium Medis adalah peer-reviewed journal yang mempublikasikan artikel-artikel ilmiah tentang ilmu kesehatan yang meliputi bidang Analis Kesehatan yang meliputi Penelitian Bakteriologi, Kimia Klinik, Parasitologi, Toksikologi Klinik, Hematologi, Sitohistoteknologi dan Imunologi, Teknologi Bank Darah. Dengan e-ISSN: 2685-8495 diterbitkan oleh Jurusan Analis Kesehatan Politeknik Kesehatan Kemenkes Semarang, terbit 6 bulan sekali, pada bulan Mei dan November setiap tahunnya.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 271 Documents
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Kasus Reaksi Transfusi pada Pasien Penerima Donor Darah di RSUD Sayang Kabupaten Cianjur Periode 2020-2022 Purlinda, Devi Etivia; Nurhamida, Tsani Nisa
Jaringan Laboratorium Medis Vol 6, No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.9899

Abstract

Blood transfusions must be performed based on appropriate indications, selecting the correct volume and type of component, and administered at the right time. The risk of transfusion reactions is inherent in nearly every transfusion process and can manifest clinical symptoms in patients. Hospitals must conduct oversight of the blood transfusion process, known as hemovigilance, to prevent transfusion reactions and enhance the safety of blood services. This study aims to determine the number of transfusion reaction cases in blood donor recipients at RSUD Sayang, Cianjur Regency, during the period from 2020 to 2022. This qualitative research utilizes a total sampling technique. The population comprises all blood donor recipients at RSUD Sayang, Cianjur Regency, from 2020 to 2022. The sample includes medical records data of transfusion reaction cases in blood donor recipients at RSUD Sayang, Cianjur Regency, from 2020 to 2022. The results indicate 35 cases of transfusion reactions. The majority of transfusion reaction cases in blood donor recipients were acute category I (mild) reactions, totaling 21 cases (60%). Patients with a history of previous transfusions predominantly experienced category I (mild) and category II (moderate) reactions, each with 12 cases (57.1%). Most acute reaction cases were category I (mild), with 20 cases (57.1%), occurring during transfusions with Packed Red Cells (PRC). The majority of acute transfusion reactions occurred after the administration of the first blood bag, with 17 cases (48.6%), and these reactions were primarily category I (mild).
Hubungan Kadar Hemoglobin dengan Infeksi Kecacingan Soil Transmitted Helminth (STH) Pada Anak Sekolah Dasar di SDN Sungai Batang 1 Kabupaten Banjar Unnufus, Ihya; Rifqoh, Rifqoh; Muhlisin, Ahmad; Kustiningsih, Yayuk
Jaringan Laboratorium Medis Vol 6, No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10347

Abstract

Worm infections are still a problem in Indonesia, one of which is South Kalimantan Province. This infection often occurs in elementary school children whose activities are often related to soil. Soil Transmitted Helminth (STH) infection in children causes intestinal inflammation, malnutrition and blood loss causing hemoglobin levels to decrease resulting in anemia. This study aims to determine the relationship between hemoglobin levels and STH worm infections in elementary school children at SDN Sungai Batang 1, Banjar Regency. This type of research is an analytical survey with a cross-sectional design. The population of this study was children in grades 1-6 at SDN Sungai Batang 1, totaling 47 people. Sample determination was carried out using total sampling technique. Hemoglobin levels were obtained from the results of examining capillary blood specimens using the Point of Care Testing (POCT) method using the Easy-Touch-GCHb tool, units gr/dL. STH worm infection is obtained from the results of microscopic examination of worm eggs in fresh fecal specimens using the Kato-Katz method with Eggs Per Gram (EPG) units. The results showed that 11 of 47 (23.41%) children were positive for STH infection, namely 5 (10.64%) Ascaris lumbricoides, 5 (10.64%) Trichuris trichiura and 1 (2.13%) were infected with both, with a total of 24 eggs. -48 EPGs. Hemoglobin levels ranged from 11.1 to 14.8 g/dL (mean 13.1 g/dL). Hemogobin levels were less than normal (12.0 gr/dL) in 11 of 47 (23.41%) children and normal hemoglobin levels in 36 (76.59%) children. The results of the Spearman rank coefficient test showed p 0.001, it can be concluded that there is a significant relationship between hemoglobin levels and STH worm infections in elementary school children at SDN Sungai Batang 1, Banjar Regency. It is recommended for future researchers to analyze the relationship between STH infection and erythrocyte index.
Efektifitas Minyak Kenanga (Cananga Odorata) sebagai Agen Deparafinisasi pada Pewarnaan Hematoxylin-Eosin Putri, Gela Setya Ayu; Diyanah, Dina; Iswara, Arya
Jaringan Laboratorium Medis Vol 6, No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10824

Abstract

Histological staining usually requires deparaffinization before starting the staining process. Deparaffinization is a paraffin removal step that often uses a xylol solution. Deparaffinization with xylol has disadvantages, including being toxic, harmful to the human body, and hazardous to the environment. A safer alternative to xylol is required. Ylang-ylang oil, which contains β-caryophyllene, is non-polar and can be used as a substitute for xylol. This study aimed to compare the staining quality of guinea pig liver tissue slides with and without heating as a deparaffinization agent in hematoxylin-eosin (HE) staining. The research set up a quasi-experimental approach using samples of guinea pig liver tissue slides. The sample size was calculated using the Federer formula (n-1) (t-1) ≥ 15, resulting in a total sample of 27 slides divided into three treatment groups: xylol, ylang-ylang oil with and without heating. The Kruskal-Wallis test was used to examine the data. The quality staining of guinea pig liver slides deparaffinized with xylol was 100% good, and deparaffinization of ylang-ylang oil with heating was 100% good, whereas deparaffinization with ylang-ylang oil without heating was 11.1% less good, and the quality was good with an 88.9%. The normality test indicated that the data were not normally distributed (p0.05). The Kruskal-Wallis test was then used.
Potensi Media Transport Modifikasi Sebagai Penunjang Pemeriksaan Kultur Bakteri Patricia, Venny; Yani, Ahmad; Nur fathonah, Amanda Kukuh
Jaringan Laboratorium Medis Vol 6, No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10574

Abstract

Bacterial culture sample transport is a critical point in the culture chain from sampling to final diagnosis. The ideal transport medium should be able to nurture the ability of bacteria to thrive coming from the sample without allowing them to grow. Transport media that already exist in Indonesia, namely in the form of powder, tend to be very expensive in price and transport media that can be used directly besides being expensive have a fast expiration period, which will increase the cost of treatment and services in hospitals, an innovation is needed by making alternative transport media from materials that are easily available around us that can be useful for taking samples. The purpose of this study is to see the potential of alternative transport media so that it can support diagnostics in bacterial culture. The study method uses an experimental laboratory with a true-experimental post-test-only design. In testing alternative transport media, sampling from diabetic foot ulcer patients using tofu pulp transport media (MTAT) and ICU swab beds with coconut charcoal transport media (MTAK). Samples of each media were taken, and as many as 20 samples were taken. In addition to treatment samples, each sample is confirmed using standard transport media as its control. This study obtained dominant bacteria from diabetic ulcer samples on alternative MTAT Citrobacter sp 6 (30%) and Control Citrobacter sp 8 (40%). Meanwhile, the ICU swab bed samples obtained dominant bacteria MTAK Bacillus sp 10 (50%) and Control 11 (55%). The conclusion of these two alternative transport media can be used as alternative transport media because they have the same growth results compared to standard media, and it can be ascertained that it is really bacteria that usually grow without contamination.
Analisis Perbedaan Nilai Hematokrit pada Penderita Hipertensi Derajat Satu dan Derajat Dua Djami, Supriati Wila; Tangkelagi, Marni; Bria, Meliance; Loaloka, Meirina Sulastri
Jaringan Laboratorium Medis Vol 6, No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10594

Abstract

Blood pressure is the pressure exerted by blood against the walls of blood vessels and is generated by the pressure of blood against the walls of arteries when the blood is pumped from the heart to the tissues. The amount of pressure varies depending on the blood vessels and heart rate. Hypertension is a non-communicable disease that triggers health problems that occur throughout the world. Kupang Regency is one of the districts with the highest cases of hypertension in NTT province with 1,582 cases. Hypertension is closely related to hemoglobin levels, hematocrit values and erythrocyte counts. There is a significant positive relationship between hematocrit values and diastolic blood pressure. Increased blood pressure has a significant positive relationship between hemoglobin levels, hematocrit values and erythrocyte counts. Apart from that, factors that influence hypertension with hemoglobin levels, hematocrit values and erythrocyte counts are age, gender, circulating fibrinogen and red blood cell aggregation. The aim of this research is to determine the difference in hematocrit values in sufferers of grade one and degree two hypertension. This research uses a cross-sectional analytical research design based on primary data originating from examination results in Bone Village, Nekamese District, Kupang Regency in April 2023 which meets the research sample criteria. Sampling for this research was determined using the Purposive Sampling technique. The research respondents were 30 hypertension sufferers in Bone Village, Nekamese District, Kupang Regency. Research on blood pressure and hematocrit values was carried out on all respondents. The results showed that hypertension sufferers consisted of 5 (17%) men and 25 (83%) women with the highest age of hypertension sufferers being 56 years old, 24 people (80%). There were 9 (30%) people suffering from grade 1 hypertension and 21 (70%) people suffering from degree 2 hypertension with the hematocrit value of most hypertensive sufferers still within normal limits, 18 (60%) and low hematocrit values as many as 12 (40%). The results of this study were processed using SPSS software with Mann-Whitney Test analysis and presented descriptively regarding the analysis of hematocrit values in sufferers of degree one and degree two hypertension. The results of statistical analysis show p-value 0.05, which means there is no significant difference between variables and vice versa. The results of the study showed that there was no difference in hematocrit values in sufferers of degree one and degree two hypertension with a value of p=0.295. In this study, hypertension sufferers generally had normal hematocrit values, but in second-degree hypertension sufferers the hematocrit values tended to be low.
Hubungan Kadar Kalium dan Kadar Ureum Terhadap Jenis Obat Baru pada Pasien Tuberkulosis Resistan Obat (TB RO) Anggita, Sasha Marwah; Qomariyah, Nurul; Kahar, Fitriani
Jaringan Laboratorium Medis Vol 6, No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.11388

Abstract

The use of new types of drugs in RO TB patients will cause disturbances in the body's electrolytes, especially potassium levels, as well as disturbances in urea levels caused by the excretion of urea that is retained in the body. Continuous increases in urea in the body result in the kidneys not being able to work optimally. The aim of this study was to determine the relationship between potassium levels and urea levels on new types of drugs in RO TB patients. The research method used is analytical observational with a cross sectional approach. This research was conducted on 16 February – 16 March 2023 with research subjects being RO TB patients who received treatment with a new type of drug at the Lung Hospital dr. Ario Wirawan Salatiga as many as 21 respondents using a sampling technique that is the total population. Potassium levels were measured using the ISE (Ion Selective Electrode) method and urea levels used the enzymatic UV test method. The data was then processed using the Shapiro-Wilk normality test, then continued with prerequisite tests (crosstab test and homogeneity test) then continued with hypothesis testing, because my data scale was ratio and nominal, the alternative test used was the Chi-Square Test. The equipment test results obtained a homogeneity test with a P value of 0.698 and 0.610 (0.05), meaning the data was homogeneous. The test results for the relationship between potassium and urea levels on new types of drugs for RO TB patients were Chi-Square hypothesis test results with a P value of 0.152 and 0.384 ( 0.05) means there is no relationship between potassium levels and urea levels on new types of drugs in RO TB patients. The results show that the TB drugs bedaquiline and delamanid do not cause kidney function disorders so that RO TB patients can consume these TB drugs regularly.
Perbedaan Kadar Vitamin D3 pada Penderita Tuberkulosis Paru Sebelum dan Sesudah Satu Bulan Terapi Obat Anti Tuberkulosis Kuncara, Rachmad Bayu; Najiah, Dewina Zumrotin
Jaringan Laboratorium Medis Vol 6, No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.11379

Abstract

Pulmonary tuberculosis is an infectious disease caused by infection with the bacteria Mycobacterium tuberculosis. Patients diagnosed with pulmonary tuberculosis will receive Anti-Tuberculosis Drug (OAT) therapy for six months. In the first two months or what is called the intensive phase, the patient will be given a combination of OAT in the form of rifampicin, isoniazid, ethambutol and pyrazinamide. Several types of anti-tuberculosis drugs have the potential to affect vitamin D3 metabolism, either by interfering with absorption, metabolism or changing the action of vitamin D3. The long-term treatment process for pulmonary tuberculosis also results in a decrease in vitamin D3 levels in the blood. This study aims to determine the difference in vitamin D3 levels in pulmonary tuberculosis sufferers before and after one month of anti-tuberculosis drug therapy. This type of research is descriptive observational with a prospective cohort design without comparison. The total sample was 16 newly diagnosed pulmonary tuberculosis sufferers at the Bangetayu, Kedungmundu and Tlogosari Wetan Health Centers, Semarang City. Vitamin D3 levels in serum samples were examined using the ELISA method. The mean vitamin D3 level before OAT therapy was 19.00 ± 10.45 ng/mL, the level decreased after OAT therapy, namely 18.51 ± 4.74 ng/mL. These results indicate that administration of anti-tuberculosis drugs may have an effect on vitamin levels.
Profil SGOT, SGPT, dan Kreatinin Pada Pasien MDR TB di RS KRMT Wongsonegoro Semarang Surati, Surati; Khairunnisa, Afifah; Setyaji, Yoki; Maulana, Mochamad Rizal
Jaringan Laboratorium Medis Vol 6, No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10951

Abstract

Tuberculosis (TB) is an infectious disease caused by infection with the bacterium Mycobacterium tuberculosis. Tuberculosis apabila is not treated immediately or the treatment is not complete can result in complications leading to death. Multidrugs resistant tuberculosis (MDR-TB) is one of the complications where the germ Mycobacterium tuberculosis can no longer bekilled with several Anti-Tuberculosis Drugs (OAT). The prevalence of MDR-TB cases in Semarang City has increased from 35 cases in 2017 to 79 cases in 2018. MDR-TB therapy takes a longer time, which is about 18-24 months. Prolonged exposure to drugs can affect body functions, especially the kidneys and liver organs which play a role in the process of removing toxins and metabolic waste. This study aims to determine the health profile of the kidney and liver organs of MDR TB sufferers through the SGOT SGPT and Creatinine parameters and describe them based on age and age range. This research is adescriptive quantitative research with a cross-sectional approach. Sampling using total samples of 18 patients. The results showed that 33% (n= 6) of patients had increased levels of SGOT and SGPT, while examination of creatinine levels showed that 33% of patients had kidney problems. Men have upnormal SGOT and SGPT levels of 40% ( n=4) compared to women, which is 25% (n=2), while upnormal creatinine levels in men are 40% (n=4) while in women it is 50% (n = 8). Patients over the age of 46 years have the most levels of SGOT SGPT and upnormal creatinine, which is 61% (n=4). The results showed that age and gender had an effect on increasing SGOT SGPT and Creatinine levels in MDR-TB patients.
Gambaran Morfologi Eritrosit Mahasiswi saat Menstruasi Setiyo Negoro, Muhammad Syamsul Arif; Septilia, Glori
Jaringan Laboratorium Medis Vol 6, No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.9926

Abstract

College students when entering the early adult phase have experienced puberty which is marked by menstruation. The duration of menstruation between one woman and another varies greatly so that the volume of blood released also varies. Excessive blood loss can reduce iron (Fe) levels in the body of female students and can affect erythrocyte morphology. Researchers conducted this study with the aim of knowing the description of erythrocyte morphology of female college students during menstruation. The research conducted is included in the type of descriptive research with observational design. The population was d3 TLM class 12 students with a sample size of 20 students who were experiencing menstruation. The sampling technique used incidental sampling. The results described the morphology of erythrocytes in female students of DIII Medical Laboratory Technology Batch XII Poltekkes Kemenkes Semarang during menstruation on the 4th day based on aspects of assessment of erythrocyte size, namely 7 people (35%) included in the microcytic category and 13 people (65%) included in the normocytic category. Based on the aspect of erythrocyte staining, 7 people (35%) were included in the hypochromic category and 13 people (65%) were included in the normochromic category. While based on the aspect of assessing the shape of erythrocytes, all 20 respondents (100%) were included in the mild poikilocytosis category. The conclusion that can be drawn from this study is the picture of erythrocyte morphology of DIII Medical Laboratory Technology Batch XII Poltekkes Kemenkes Semarang students during menstruation on the 4th day, out of 20 respondents as many as 7 people (35%) showed hypochromic microcytic erythrocyte morphological abnormalities with mild poikilocytosis and 13 people (65%) showed normocytic normochromic erythrocyte morphological abnormalities with mild poikilocytosis. It is recommended to increase the consumption of vegetables and foods that increase the formation of red blood cells during menstruation.
Gambaran Kadar Hemoglobin pada Calon pendonor Usia lebih Dari 46 Tahun di UDD PMI Kabupaten Jepara Luthfia, Alya; Wikandari, Ririh Jatmi; Widodo, Widodo
Jaringan Laboratorium Medis Vol 6, No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.11437

Abstract

Blood donation is the process of taking a portion of blood from a healthy person then giving it to the person who needs blood. Hemoglobin level examination is one of the donor selection checks to find out whether someone is successful or not to donate blood. A person can donate blood if the hemoglobin level shows a result of 12.5 g / dL – 17 g / dL. A person's hemoglobin level can be influenced by age, because as we get older many organ functions that do not work optimally so that they experience a decrease in Hb levels. The purpose of this study was to measure hemoglobin levels in prospective donors aged more than 46 years at UDD PMI Jepara Regency. This type of research uses a quantitative approach with a cross-sectional descriptive study research model taken from the SIMDONDAR database. The population in this study is all potential donors at UDD PMI Jepara Regency in December 2023-January 2024. The data obtained were analyzed univariately. Based on the results of the study, it was found that the majority of prospective donors showed normal hemoglobin levels as many as 480 prospective donors (94.86%), and more prospective donors who had blood type O (+) as many as 187 prospective donors (36.96%), most prospective donors were male as many as 342 prospective donors (67.59%), and the majority of prospective donors were declared to have passed the donor selection as many as 480 prospective donors (94.86%). The impact of the research results obtained by UDD PMI Jepara Regency is that officers are aware of the importance of socializing to the community how important it is to maintain hemoglobin levels before donating blood and recruiting donors from various ages of prospective donors in the Jepara Regency area.

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