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INDONESIA
Jaringan Laboratorium Medis
ISSN : -     EISSN : 26858495     DOI : -
Core Subject : Health, Science,
Jurnal Online Jaringan Laboratorium Medis adalah peer-reviewed journal yang mempublikasikan artikel-artikel ilmiah tentang ilmu kesehatan yang meliputi bidang Analis Kesehatan yang meliputi Penelitian Bakteriologi, Kimia Klinik, Parasitologi, Toksikologi Klinik, Hematologi, Sitohistoteknologi dan Imunologi, Teknologi Bank Darah. Dengan e-ISSN: 2685-8495 diterbitkan oleh Jurusan Analis Kesehatan Politeknik Kesehatan Kemenkes Semarang, terbit 6 bulan sekali, pada bulan Mei dan November setiap tahunnya.
Articles 270 Documents
Hubungan Kadar Hemoglobin dengan Infeksi Kecacingan Soil Transmitted Helminth (STH) Pada Anak Sekolah Dasar di SDN Sungai Batang 1 Kabupaten Banjar Unnufus, Ihya; Rifqoh, Rifqoh; Muhlisin, Ahmad; Kustiningsih, Yayuk
Jaringan Laboratorium Medis Vol. 6 No. 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10347

Abstract

Worm infections are still a problem in Indonesia, one of which is South Kalimantan Province. This infection often occurs in elementary school children whose activities are often related to soil. Soil Transmitted Helminth (STH) infection in children causes intestinal inflammation, malnutrition and blood loss causing hemoglobin levels to decrease resulting in anemia. This study aims to determine the relationship between hemoglobin levels and STH worm infections in elementary school children at SDN Sungai Batang 1, Banjar Regency. This type of research is an analytical survey with a cross-sectional design. The population of this study was children in grades 1-6 at SDN Sungai Batang 1, totaling 47 people. Sample determination was carried out using total sampling technique. Hemoglobin levels were obtained from the results of examining capillary blood specimens using the Point of Care Testing (POCT) method using the Easy-Touch-GCHb tool, units gr/dL. STH worm infection is obtained from the results of microscopic examination of worm eggs in fresh fecal specimens using the Kato-Katz method with Eggs Per Gram (EPG) units. The results showed that 11 of 47 (23.41%) children were positive for STH infection, namely 5 (10.64%) Ascaris lumbricoides, 5 (10.64%) Trichuris trichiura and 1 (2.13%) were infected with both, with a total of 24 eggs. -48 EPGs. Hemoglobin levels ranged from 11.1 to 14.8 g/dL (mean 13.1 g/dL). Hemogobin levels were less than normal (<12.0 gr/dL) in 11 of 47 (23.41%) children and normal hemoglobin levels in 36 (76.59%) children. The results of the Spearman rank coefficient test showed p < 0.001, it can be concluded that there is a significant relationship between hemoglobin levels and STH worm infections in elementary school children at SDN Sungai Batang 1, Banjar Regency. It is recommended for future researchers to analyze the relationship between STH infection and erythrocyte index.
The Relationship of Zink and Iron Levels with Blood Pressure in Pulmonary Tuberculosis Patient Kurniasih, Dewi; Muslina, Muslina; Latifah, Aminahtun; Afrianti, Dina
Jaringan Laboratorium Medis Vol. 5 No. 2 (2023): November 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i2.10415

Abstract

Background: Mycobacterium tuberculosis infection in Tuberculosis (TBC) causes narrowing of the pulmonary blood vessels and triggers pulmonary hypertension which is associated with iron deficiency and anemia. The mineral zinc (Zn) is known to play an important role in destroying the bacteria that cause TBC. In addition, iron (Fe) deficiency in TBC patients can cause relapse and even treatment failure. This study aims to examine the relationship between serum zinc and iron levels and blood pressure in pulmonary TBC patients. Method: A descriptive analytical observational study with a cross sectional design was conducted on 51 TBC patients undergoing treatment at the Simpang IV Sipin Community Health Center, Jambi City. Using a purposive sampling technique, respondents who met the research inclusion and exclusion criteria were examined for zinc and Fe levels. The examination was carried out at the Jambi Province Regional Health Laboratory, the examination result data was then analyzed bivariately using the chi square test. Results: The results of statistical analysis between blood pressure and Zn levels obtained a significant value with P value = 0.877 and a significant value for Fe levels obtained P value = 0.841 (> 0.05). Conclusion: There is no significant relationship between Zn and Fe levels and blood pressure in pulmonary TBC patients. Based on research, it is known that iron deficiency and anemia are not directly related to pulmonary hypertension which can be experienced by TBC patients.
Analisis Kadar Karboksihemoglobin (COHb) pada Montir Motor di Area Kota Samarinda Wahid, Rifky Saldi A.; Kaperius, Georgius; Rasydy, La Ode Akbar
Jaringan Laboratorium Medis Vol. 5 No. 2 (2023): November 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i2.10526

Abstract

The quality of air is very important for the environment and human life, however, along with developments in today's increasingly developed and modern era, the quality of the air itself is getting worse and the substances that become air pollutants, one of which is carbon monoxide. Exposure to high amounts of carbon monoxide gas over a continuous exposure period can of course increase carboxyhemoglobin levels in the blood, which will cause health problems. To determine the relationship between carboxyhemoglobin levels in the blood of motor vehicle mechanic workers in the Samarinda city area based on factors such as age, length of work, length of service, and smoking habits. This research used a cross sectional analytical survey design, and sample measurements used the Hindsberg-Lang method which was carried out in May 2023 with a sample size of 17 people and a Spearman rank statistical test was carried out to see whether there was a relationship between variables. 17 motor vehicle mechanics had COHb concentrations that exceeded the normal limit of <3.5%, where the lowest carboxyhemoglobin level was 6.10% and the highest was 9.64%. Correlation data obtained from the p value of age (p=0.000), length of service (p=0.000), time worked (p=0.000), and smoking habits (p=0.004) on COHb levels in the blood of motor vehicle mechanics. The Conclusion: COHb levels in motor vehicle mechanics are above 3.5% and there is a significant relationship between age, length of service, working time and smoking habits on COHb levels in the blood of motor vehicle mechanics.
Potensi Media Transport Modifikasi Sebagai Penunjang Pemeriksaan Kultur Bakteri Patricia, Venny; Yani, Ahmad; Nur fathonah, Amanda Kukuh
Jaringan Laboratorium Medis Vol. 6 No. 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10574

Abstract

Bacterial culture sample transport is a critical point in the culture chain from sampling to final diagnosis. The ideal transport medium should be able to nurture the ability of bacteria to thrive coming from the sample without allowing them to grow. Transport media that already exist in Indonesia, namely in the form of powder, tend to be very expensive in price and transport media that can be used directly besides being expensive have a fast expiration period, which will increase the cost of treatment and services in hospitals, an innovation is needed by making alternative transport media from materials that are easily available around us that can be useful for taking samples. The purpose of this study is to see the potential of alternative transport media so that it can support diagnostics in bacterial culture. The study method uses an experimental laboratory with a true-experimental post-test-only design. In testing alternative transport media, sampling from diabetic foot ulcer patients using tofu pulp transport media (MTAT) and ICU swab beds with coconut charcoal transport media (MTAK). Samples of each media were taken, and as many as 20 samples were taken. In addition to treatment samples, each sample is confirmed using standard transport media as its control. This study obtained dominant bacteria from diabetic ulcer samples on alternative MTAT Citrobacter sp 6 (30%) and Control Citrobacter sp 8 (40%). Meanwhile, the ICU swab bed samples obtained dominant bacteria MTAK Bacillus sp 10 (50%) and Control 11 (55%). The conclusion of these two alternative transport media can be used as alternative transport media because they have the same growth results compared to standard media, and it can be ascertained that it is really bacteria that usually grow without contamination.
Desain Primer untuk Deteksi Gen Diphtheria Toxin Repressor (dtxR) sebagai Biomarker Bakteri Corynebacterium diphtheriae Menggunakan In Silico PCR Nastiti, Hilari Rio Rosa; Kuncara, Rachmad Bayu
Jaringan Laboratorium Medis Vol. 5 No. 2 (2023): November 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i2.10588

Abstract

Corynebacterium diphtheriae is the bacteria that causes diphtheria. The virulence factor of C. diphtheriae comes from the bacteria's ability to produce bacterial toxins. Toxin production is regulated by a set of genes called tox/dtx genes and is regulated by the dtxR gene. The aim of this study was to design primers used to evaluate the dtxR gene using bacterial DNA sequences. This research is experimental research with a literature study approach using the In silico Polymerase Chain Reaction (PCR), NCBI (National Center for Biotechnology Information), Primer3Plus, and Oligo Calculator applications. The sample obtained from genbank NCBI was C. diphtheriae dtxR gene M80337.1. In silico PCR examination was carried out using newly designed primers from Primer3Plus with 50 genomic DNA of Corynebacterium spp. taken from the In silico PCR database. The dtxR primer pair: '5-ACAGTTAGCCAAACCGTTGC-3' and 5'-TGCGTTCAACTTCGTCACTC-3' can produce a single DNA amplicon measuring 226 bp specifically for C. diphtheria types and no amplicon bands were generated from other Corynebacterium genomes. Based on the study results, this pair of specific primers can be used for in vitro PCR testing and can be used to develop rapid detection of diphtheria.
Analisis Perbedaan Nilai Hematokrit pada Penderita Hipertensi Derajat Satu dan Derajat Dua Djami, Supriati Wila; Tangkelagi, Marni; Bria, Meliance; Loaloka, Meirina Sulastri
Jaringan Laboratorium Medis Vol. 6 No. 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10594

Abstract

Blood pressure is the pressure exerted by blood against the walls of blood vessels and is generated by the pressure of blood against the walls of arteries when the blood is pumped from the heart to the tissues. The amount of pressure varies depending on the blood vessels and heart rate. Hypertension is a non-communicable disease that triggers health problems that occur throughout the world. Kupang Regency is one of the districts with the highest cases of hypertension in NTT province with 1,582 cases. Hypertension is closely related to hemoglobin levels, hematocrit values and erythrocyte counts. There is a significant positive relationship between hematocrit values and diastolic blood pressure. Increased blood pressure has a significant positive relationship between hemoglobin levels, hematocrit values and erythrocyte counts. Apart from that, factors that influence hypertension with hemoglobin levels, hematocrit values and erythrocyte counts are age, gender, circulating fibrinogen and red blood cell aggregation. The aim of this research is to determine the difference in hematocrit values in sufferers of grade one and degree two hypertension. This research uses a cross-sectional analytical research design based on primary data originating from examination results in Bone Village, Nekamese District, Kupang Regency in April 2023 which meets the research sample criteria. Sampling for this research was determined using the Purposive Sampling technique. The research respondents were 30 hypertension sufferers in Bone Village, Nekamese District, Kupang Regency. Research on blood pressure and hematocrit values was carried out on all respondents. The results showed that hypertension sufferers consisted of 5 (17%) men and 25 (83%) women with the highest age of hypertension sufferers being > 56 years old, 24 people (80%). There were 9 (30%) people suffering from grade 1 hypertension and 21 (70%) people suffering from degree 2 hypertension with the hematocrit value of most hypertensive sufferers still within normal limits, 18 (60%) and low hematocrit values as many as 12 (40%). The results of this study were processed using SPSS software with Mann-Whitney Test analysis and presented descriptively regarding the analysis of hematocrit values in sufferers of degree one and degree two hypertension. The results of statistical analysis show p-value > 0.05, which means there is no significant difference between variables and vice versa. The results of the study showed that there was no difference in hematocrit values in sufferers of degree one and degree two hypertension with a value of p=0.295. In this study, hypertension sufferers generally had normal hematocrit values, but in second-degree hypertension sufferers the hematocrit values tended to be low.
Efektifitas Minyak Kenanga (Cananga Odorata) sebagai Agen Deparafinisasi pada Pewarnaan Hematoxylin-Eosin Putri, Gela Setya Ayu; Diyanah, Dina; Iswara, Arya
Jaringan Laboratorium Medis Vol. 6 No. 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10824

Abstract

Histological staining usually requires deparaffinization before starting the staining process. Deparaffinization is a paraffin removal step that often uses a xylol solution. Deparaffinization with xylol has disadvantages, including being toxic, harmful to the human body, and hazardous to the environment. A safer alternative to xylol is required. Ylang-ylang oil, which contains β-caryophyllene, is non-polar and can be used as a substitute for xylol. This study aimed to compare the staining quality of guinea pig liver tissue slides with and without heating as a deparaffinization agent in hematoxylin-eosin (HE) staining. The research set up a quasi-experimental approach using samples of guinea pig liver tissue slides. The sample size was calculated using the Federer formula (n-1) (t-1) ≥ 15, resulting in a total sample of 27 slides divided into three treatment groups: xylol, ylang-ylang oil with and without heating. The Kruskal-Wallis test was used to examine the data. The quality staining of guinea pig liver slides deparaffinized with xylol was 100% good, and deparaffinization of ylang-ylang oil with heating was 100% good, whereas deparaffinization with ylang-ylang oil without heating was 11.1% less good, and the quality was good with an 88.9%. The normality test indicated that the data were not normally distributed (p<0.05). The Kruskal-Wallis test was then used.
Gambaran Stabilitas Pooled plasma CPDA Sebagai Bahan Kontrol Kualitas Pemeriksaan Asam Urat Pada Uji Presisi Within run dan Between day Setyaji, Yoki; Duri, Iin Desmiany; Setyowatiningsih, Lilik
Jaringan Laboratorium Medis Vol. 6 No. 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.10933

Abstract

The quality of laboratory test results is ensured by implementing quality control measures, which usually require control materials. The high cost of commercial control materials poses a challenge for laboratory quality control. Commonly, laboratory control materials are commercial control serums derived from animal serum that undergoes lyophilization during manufacturing. This study aims to assess the stability of CPDA pooled plasma that has been frozen for 60 days, using precision tests both within-run and between-day. Methods: This experimental research utilized CPDA pooled plasma samples from hematology laboratory waste at Poltekkes Kemenkes Semarang. The samples were frozen at -20℃ for 60 days. Quality control (QC) analysis was conducted using within-run and between-day precision tests without replication, to determine the standard deviation (SD) and coefficient of variation (CV). The results were then statistically analyzed using a one-way ANOVA to assess the difference in CPDA pooled plasma stability between the within-run and between-day precision tests. The QC results for within-day and between-day precision were as follows: the average for within-day was 4.066 and for between-day 3.2785; SD for within-day was 0.20 and for between-day 0.09; CV for within-day was 4.9% and for between-day 2.9%. The one-way ANOVA test showed a significance value of 0.316, indicating no significant difference between the within-day and between-day QC methods in terms of precision testing. Conclusion: CPDA pooled plasma remains stable in maintaining the precision of QC results for uric acid using the enzymatic colorimetric method (end-point) after 60-80 days of storage and can be used as an alternative to commercial control materials in uric acid QC testing.
Profil SGOT, SGPT, dan Kreatinin Pada Pasien MDR TB di RS KRMT Wongsonegoro Semarang Surati, Surati; Khairunnisa, Afifah; Setyaji, Yoki; Maulana, Mochamad Rizal
Jaringan Laboratorium Medis Vol. 6 No. 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10951

Abstract

Tuberculosis (TB) is an infectious disease caused by infection with the bacterium Mycobacterium tuberculosis. Tuberculosis apabila is not treated immediately or the treatment is not complete can result in complications leading to death. Multidrugs resistant tuberculosis (MDR-TB) is one of the complications where the germ Mycobacterium tuberculosis can no longer bekilled with several Anti-Tuberculosis Drugs (OAT). The prevalence of MDR-TB cases in Semarang City has increased from 35 cases in 2017 to 79 cases in 2018. MDR-TB therapy takes a longer time, which is about 18-24 months. Prolonged exposure to drugs can affect body functions, especially the kidneys and liver organs which play a role in the process of removing toxins and metabolic waste. This study aims to determine the health profile of the kidney and liver organs of MDR TB sufferers through the SGOT SGPT and Creatinine parameters and describe them based on age and age range. This research is adescriptive quantitative research with a cross-sectional approach. Sampling using total samples of 18 patients. The results showed that 33% (n= 6) of patients had increased levels of SGOT and SGPT, while examination of creatinine levels showed that 33% of patients had kidney problems. Men have upnormal SGOT and SGPT levels of 40% ( n=4) compared to women, which is 25% (n=2), while upnormal creatinine levels in men are 40% (n=4) while in women it is 50% (n = 8). Patients over the age of 46 years have the most levels of SGOT SGPT and upnormal creatinine, which is 61% (n=4). The results showed that age and gender had an effect on increasing SGOT SGPT and Creatinine levels in MDR-TB patients.
Perbedaan Kadar Vitamin D3 pada Penderita Tuberkulosis Paru Sebelum dan Sesudah Satu Bulan Terapi Obat Anti Tuberkulosis Kuncara, Rachmad Bayu; Najiah, Dewina Zumrotin
Jaringan Laboratorium Medis Vol. 6 No. 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.11379

Abstract

Pulmonary tuberculosis is an infectious disease caused by infection with the bacteria Mycobacterium tuberculosis. Patients diagnosed with pulmonary tuberculosis will receive Anti-Tuberculosis Drug (OAT) therapy for six months. In the first two months or what is called the intensive phase, the patient will be given a combination of OAT in the form of rifampicin, isoniazid, ethambutol and pyrazinamide. Several types of anti-tuberculosis drugs have the potential to affect vitamin D3 metabolism, either by interfering with absorption, metabolism or changing the action of vitamin D3. The long-term treatment process for pulmonary tuberculosis also results in a decrease in vitamin D3 levels in the blood. This study aims to determine the difference in vitamin D3 levels in pulmonary tuberculosis sufferers before and after one month of anti-tuberculosis drug therapy. This type of research is descriptive observational with a prospective cohort design without comparison. The total sample was 16 newly diagnosed pulmonary tuberculosis sufferers at the Bangetayu, Kedungmundu and Tlogosari Wetan Health Centers, Semarang City. Vitamin D3 levels in serum samples were examined using the ELISA method. The mean vitamin D3 level before OAT therapy was 19.00 ± 10.45 ng/mL, the level decreased after OAT therapy, namely 18.51 ± 4.74 ng/mL. These results indicate that administration of anti-tuberculosis drugs may have an effect on vitamin levels.