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Jaringan Laboratorium Medis
Published by Poltekkes Kemenkes Semarang
ISSN : -     EISSN : 26858495     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Public Health
Jurnal Online Jaringan Laboratorium Medis adalah peer-reviewed journal yang mempublikasikan artikel-artikel ilmiah tentang ilmu kesehatan yang meliputi bidang Analis Kesehatan yang meliputi Penelitian Bakteriologi, Kimia Klinik, Parasitologi, Toksikologi Klinik, Hematologi, Sitohistoteknologi dan Imunologi, Teknologi Bank Darah. Dengan e-ISSN: 2685-8495 diterbitkan oleh Jurusan Analis Kesehatan Politeknik Kesehatan Kemenkes Semarang, terbit 6 bulan sekali, pada bulan Mei dan November setiap tahunnya.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 281 Documents
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Perbandingan Pemeriksaan Skrining Antibodi (Indirect Coombs Test) dengan Direct Coombs Test pada Pasien dengan Riwayat Transfusi Darah Berulang Shela Yuni Arta; Suhariyadi Suhariyadi; Evy Diah Woelansari; Christ Kartika Rahayuningsih
Jaringan Laboratorium Medis Vol 7 No 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13643

Abstract

Repeated blood transfusions can increase risk of alloimmunization and autoimunization, which causes formation alloantibodies and autoantibodies. The presence of these antibodies can make cross-matching more difficult and increase the possibility of hemolytic transfusion risk. Antibody screening tests (Indirect Coombs Test) and Direct Coombs Test play a role in detecting the presence of alloantibodies and autoantibodies in patient with a history of repeated transfusion. The aim of the study was to determine the diferences in results between antibody screening (Indirect Coombs Test) and Direct Coombs Test in patients receiving repeated blood transfusions. This study used a quasi-experimental design with a cross-sectional approach. The subjects were patients with a history of blood transfusions more than 5 times at RSUD R.T. Notopuro Sidoarjo totalling 60 samples. The data obtained were then analyzed statistically to see if there were any significant differences between the two types of examinations. The results obtained showed a significant difference between the results of the antibody screening test (ICT) and the Direct Coombs Test (DCT) (p-value 0.000), that more patients with repeated blood transfusions had positive DCT results (75%). Some patients showed positive results on one or both types of tests, indicating the presence of alloantibodies or autoantibodies. ICT and DCT tests provide complementary information in detecting antibodies in patients with repeated transfusions. The use of both is important in improving the safety of blood transfusions and minimizing the risk of hemolytic reactions.
Rasio Neutrofil-Limfosit (NLR) sebagai Penanda Disfungsi Hati pada Pendonor Darah Sukarela di Unit Transfusi Darah Palang Merah Indonesia Qurrotu A'yuni Auliya; Ririh Jatmi Wikandari; Bambang Supriyanta; Duwi Pudjiastuti; Yunita Rusidah
Jaringan Laboratorium Medis Vol 8 No 1 (2026): May 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i1.14587

Abstract

Background: HBsAg is a marker for hepatitis B virus, which causes liver dysfunction characterized by elevated serum glutamic pyruvic transaminase (SGPT) levels. The neutrophil-lymphocyte ratio (NLR) has a positive effect on SGPT. Objective: To describe NLR as a marker of liver dysfunction in voluntary blood donors at the PMI Blood Donation Unit (UDD). Methods: Descriptive study to determine the role of NLR as a marker of liver dysfunction in voluntary blood donors. The study population consisted of voluntary blood donors at the PMI Blood Donation Unit in Salatiga, Central Java. Primary data collected through complete blood tests and SGPT measurements. Secondary data were HBsAg results in voluntary donors. Results: All 20 respondents (100%) had an average SGPT level of 20 U/L and non-reactive HBsAg results, with an average NLR value of 1.76. The highest age group was 26-35 years old, with 8 respondents (40%). Based on gender, 15 respondents (75%) were male and 5 respondents (25%) were female. The percentage of blood types A and O was 8 respondents (40%), while blood types B and AB were represented by 2 respondents (10%). Conclusions: NLR cannot be used as a marker of liver dysfunction based on SGPT and HBsAG parameters.
Optimasi Variasi Konsentrasi Asam Trikloroasetat dalam Penanganan Sampel Ikterik pada Pemeriksaan Kadar Asam Urat Metode Uricase Sulthan Abyan Surya Durachman; Ani Riyani; Dhini Annisa Rahmasari Kanto; Dewi Nurhayati
Jaringan Laboratorium Medis Vol 8 No 1 (2026): May 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i1.14414

Abstract

Uric acid examination of the uricase method can be disturbed due to bilirubin in icteric serum. Bilirubin absorbs light at a similar wavelength to uricase reaction products, so it can cause gout results to appear higher than they actually are. To overcome this, proper pre-analytical handLing is needed with the addition of trichloroacetic acid (TCA). The purpose of this study was to determine the difference in uric acid levels before and after the addition of TCA to icteric serum and to determine the optimal concentration of TCA to reduce bilirubin disorders in uric acid examination. This research method uses a quasi-experiment using pooled sera that were recognized using catfish bile, with bilirubin levels of 2.13; 2.83; and 3.96 mg/dL. Each sample was treated with TCA at concentrations of 4%, 5%, and 6%. Uric acid levels were checked using the uricase method and data were analyzed using ANOVA and Post Hoc tests. The results showed a decrease in uric acid levels as TCA concentrations increased. The concentration of 6% TCA provided the most significant reduction, but the 5% TCA at bilirubin level of 2.83 mg/dL showed no significant difference compared to normal pooled sera (p > 0.05), suggesting that 5% TCA was the optimal concentration in the handLing of icteric samples on uric acid examination.
Uji MPN Coliform pada Air Isi Ulang dengan Sterilisasi Sinar UV Widodo Widodo; Dian Yulia Purwanti; S.Y Didik Widiyanto; Devi Etevia Purlinda
Jaringan Laboratorium Medis Vol 8 No 1 (2026): May 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i1.14460

Abstract

Wolter Refill water typically undergoes a filtration and sterilization process using 254 nm UV light to sterilize against microbial contamination. This bacterial contamination can be tested using the MPN Coliform test, which is the most important microbiological parameter for drinking water quality. Fecal coliform bacteria are the most commonly used microorganisms as indicators of water pollution, as these bacteria are always present in feces. According to Indonesian Minister of Health Regulation No. 492/MENKES/PER/IV/2010, mandatory standards for drinking water quality are regulated, including physical and microbiological requirements. The purpose of this study was to identify the presence of coliform and fecal coliform bacteria in AMIU sold along Jalan Wolter Monginsidi, Semarang, and to analyze their presence in drinking water. The research method was descriptive. The object of the study was AMIU from eight AMIU depots sold along Jalan Wolter Monginsidi, Semarang, while the subjects were coliform and fecal coliform bacteria. The data collected consists of primary data and supporting data which are then arranged descriptively. Research results There are 3 AMIU depots that are positive for containing Coliform bacteria, namely Depot A4, A5 and Depot A8 with a total Coliform bacteria content of 2 MPN / 100 ml. While the 8 AMIU depots studied did not contain fecal Coliform bacteria, namely 0 MPN / 100 ml. The causes of the presence of Coliform bacteria in positive AMIU samples include the sterilization method only using UV light, the sterilization time is less than 1 hour, the sterilization tool is not replaced periodically, the AMIU storage time at the depot is more than 3 days, the gallon is not washed properly, and the AMIU Laboratory is not checked routinely. Poor results in the test are submitted to the depot manager for follow-up by resetting the sterilization tool.
Hubungan Antara Higienitas Helm dengan Pertumbuhan Jamur Dermatofita pada Helm Pengemudi Ojek di Kawasan Wisata Religi Wahyu Ari Setiyawan; Indanah; Navira Septiana Ulfa
Jaringan Laboratorium Medis Vol 8 No 1 (2026): May 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i1.14502

Abstract

Dermatophyte fungal infections are one of the most common contagious skin diseases in the community, especially on the scalp, hair, and nails. Transmission can occur indirectly through objects, one of which is helmets. Motorcycle taxi drivers in tourist areas are at high risk of fungal contamination due to the massive use of helmets. This study aims to determine the relationship between helmet hygiene levels and dermatophyte fungal growth on motorcycle taxi driver helmets in religious tourist areas. The type of research used is observational analytical with a cross-sectional approach. The research sample consisted of 37 motorcycle taxi drivers selected using purposive sampling. Helmet hygiene data was obtained through a questionnaire, which found that most respondents had good hygiene (78.4%) and 8 respondents (21.6%) had poor hygiene. The results of dermatophyte fungal growth were tested using culture on SDA medium, revealing that the majority of samples were negative (30 respondents, 81.1%), while 7 samples were positive for dermatophytes (18.9%) from the genera Microsporum sp. and Trichophyton sp., using LPCB staining. Data analysis was performed univariately and bivariately using Fisher's Exact test. The results of Fisher's Exact test showed a significant relationship between helmet hygiene and dermatophyte fungal growth (p < 0.001) with an odds ratio of 84.000. The conclusion of this study indicates that poor helmet hygiene can increase the risk of dermatophyte fungal contamination, which causes tinea capitis, in religious tour motorcycle taxi drivers.
Studi Perbandingan Variasi ph Buffer Fosfat Pada Kualitas Pewarnaan Giemsa Terhadap Hitung Jenis Leukosit Hesty Aulya Putri; Anisa Solikhati
Jaringan Laboratorium Medis Vol 8 No 1 (2026): May 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i1.14559

Abstract

Blood is an important fluid in the body that functions to circulate nutrients, carry metabolic waste, and play a role in the body's defense system. Leukocytes, especially neutrophils and lymphocytes, are white blood cells that play a major role in the immune response. One of the hematology tests often performed to assess the morphology and percentage of leukocytes is the examination of peripheral blood smears with Giemsa staining. The quality of Giemsa staining results is influenced by the pH of the phosphate buffer, because a pH that is too acidic or alkaline can cause the color of the nucleus and cytoplasm of cells to become unclear. This study aims to determine the effect of differences in phosphate buffer pH on the quality of Giemsa staining in leukocyte differential count examinations. This study used a quasi-experimental design with a quantitative approach and was conducted in January 2025 at the Medical Laboratory Technology Laboratory of the Muhammadiyah University of Kudus. The independent variable was the pH of the phosphate buffer with variations of pH 4, 7, and 8, while the dependent variable was the quality of Giemsa staining on leukocyte differential counts. The research sample consisted of 9 final year students selected using random sampling technique, with each sample receiving three different phosphate buffer pH treatments. Data analysis was performed using the Kruskal-Wallis test because the data were not normally distributed and showed significant differences between pH groups. Further Mann-Whitney tests showed significant differences between pH 7 and pH 8 groups. The results indicate that variations in phosphate buffer pH affect the quality of Giemsa staining and leukocyte count accuracy.
Perbandingan Efektivitas Metode Natif Dan Sedimentasi Dalam Deteksi Telur Cacing Ascaris Lumbricoides Di TPS Kecamatan Gebog Ririn Handayani; Shinta Dwi Kurnia
Jaringan Laboratorium Medis Vol 8 No 1 (2026): May 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i1.14593

Abstract

The World Health Organization (WHO) reports that more than 1.5 billion people are infected with soil-transmitted helminths (STH). Ascaris lumbricoides infection remains a health problem in areas with poor sanitation, such as waste disposal sites (TPS). Detection of helminth eggs in fecal samples is important to determine the level of environmental contamination and the effectiveness of prevention. The aim of this study is to improve the effectiveness of parasitological examinations in laboratories through the use of more specific methods. This study is motivated by the still high risk of helminth infections, especially Ascaris lumbricoides, in environments with poor sanitation such as temporary disposal sites (TPS) and to determine the effectiveness of native and sedimentation methods in detecting Ascaris lumbricoides eggs in fecal samples of TPS officers in Gebog District. This study uses a laboratory experimental method with a comparative approach. Fecal samples were taken from potentially contaminated TPS officers, then examined using the Native method (direct examination) and Sedimentation (with 0.9% NaCl solution). Parameters considered included the number and shape of Ascaris lumbricoides eggs found, as well as the efficiency of each method. The data analysis used McNemar statistics. The results showed no statistically significant difference between the native and sedimentation methods. This means that both methods have nearly equal ability to detect Ascaris lumbricoides eggs in the fecal samples of polling station officers in Gebog District.The parameters observed included the number and shape of Ascaris lumbricoides worm eggs found, as well as the efficiency of each method. Data analysis used Wilcoxon statistics. The results of this study The Wilcoxon test results show that of the 27 fecal samples, N = 0 (Sedimentation < Native) was found, this indicates that no sample produced better results from both methods, either the native or sedimentation method. In the comparison category N = 1 (Sedimentation > Native) this shows that there is 1 sample that was detected by the sedimentation method better than the native method. While the results of the Sedimentation = Native (Ties) test mean that some samples 26 out of 27 showed the same results from both methods. A p value of 0.317 (p > 0.5) was obtained. These results indicate that there is no statistically significant difference between the native method and the sedimentation method. This means that both methods have almost the same ability to detect Ascaris lumbricoides eggs in the feces samples of polling station officers in Gebog District.
Perbandingan Kadar Natrium Serum pada Pasien Gagal Ginjal dan Non-Gagal Ginjal Menggunakan Elektrolit Analyzer Cornley Nurul Siamtryningsih; Yayuk Mundriyastutik; Arief Adi Saputro
Jaringan Laboratorium Medis Vol 8 No 1 (2026): May 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i1.14602

Abstract

Kidney failure is the inability of the kidneys to filter blood and maintain electrolyte balance, particularly sodium, which plays an important role in regulating body fluids and neuromuscular activity. Sodium imbalance, such as hyponatremia, often occurs in patients with kidney failure due to impaired excretion, thereby increasing the risk of neurological and cardiovascular complications. This study aimed to compare serum sodium levels between patients with kidney failure and those without kidney failure using the Cornley Electrolyte Analyzer.This research employed a quantitative approach with a cross-sectional design, involving a random sample of 88 respondents (44 patients with kidney failure and 44 without kidney failure) at Al-Ikhlas Islamic Hospital, Pemalang, without considering gender, aged 18–70 years. Serum sodium levels were measured using the ion-selective electrode (ISE) technique. Data analysis included univariate analysis (descriptive statistics and Shapiro-Wilk normality test) and bivariate analysis (Mann-Whitney U test) at a significance level of α=0.05. The results showed that the median serum sodium level in kidney failure patients was 134.5 mmol/L (IQR=9), indicating a higher tendency toward hyponatremia, while non-kidney failure patients had a mean of 137.58 mmol/L (SD=6.70), reflecting greater stability. Statistical analysis demonstrated a significant difference (p=0.014), with higher sodium levels observed in the non-kidney failure group. In conclusion, there is a significant difference in serum sodium levels between the two groups, where patients with kidney failure are more prone to hyponatremia. Therefore, routine electrolyte monitoring and adherence to a low-sodium diet are necessary.
Profil Lipid Perokok Konvensional pada Pekerja Bengkel Motor di Jalan Wolter Monginsidi Semarang Nurul Qomariyah; Fitriani Kahar; Iin Desmiany Duri; Aqilah Defri Ramadhanty; Fadillah Zaskia Putri; Aqilla Hasnu Rajwa
Jaringan Laboratorium Medis Vol 8 No 1 (2026): May 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i1.14632

Abstract

Cardiovascular disease is the leading cause of death globally and one of the main risk factors is impaired lipid profiles due to smoking habits (Naomi et al., 2021). Smoking has a significant effect on lipid metabolism. Several studies have shown that smoking habits can cause increased levels of total cholesterol, LDL (Low Density Lipoprotein), and triglycerides, and decreased levels of HDL (High Density Lipoprotein) (Amalia, n.d.) 2018. This imbalance contributes to the risk of cardiovascular disease, atherosclerosis and coronary heart disease (Amalia, n.d.). This study aims to determine the lipid profile of conventional smokers in motorcycle repair shop workers on Jalan Wolter Monginsidi Semarang Method: This study is a descriptive quantitative study with a cross-sectional approach. Analysis Results Lipid profile levels of conventional smokers in motorcycle repair shop workers on Jalan Wolter Munginsidi total cholesterol levels showed significant differences between smoker groups with a p value = 0.010. The average total cholesterol level in light smokers was 141.92±18.97 mg/dL, moderate smokers 150.76±26.86 mg/dL, and heavy smokers 207.70±50.13 mg/dL. This indicates that the heavier the smoker classification, the higher the total cholesterol level. For triglyceride, LDL, and HDL levels, although there were differences in the mean between groups, the statistical test results did not show a significant difference (p>0.05). This indicates that the number of cigarettes consumed per day has a greater influence on increasing total cholesterol levels than other lipid parameters. Conclusion: The number of cigarettes consumed per day has a greater influence on increasing total cholesterol levels than other lipid parameters.
Perbedaan Jumlah Eritrosit Sebelum dan Sesudah Donor Darah di Rumah Sakit Muhammadiyah Mardhatillah Randudongkal Pemalang Anggia Lestari; Yunita Rusidah
Jaringan Laboratorium Medis Vol 8 No 1 (2026): May 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i1.14715

Abstract

Blood donation is a humanitarian activity that plays an important role in meeting national blood supply needs; however, it may cause temporary changes in hematological parameters, including erythrocyte count. This study aimed to analyze the difference in erythrocyte count before and 60 minutes after blood donation among voluntary donors. This research used a pre-experimental quantitative design with a One Group Pretest–Posttest approach. A total of 39 voluntary donors were selected using a purposive sampling technique. Erythrocyte counts were measured using an automated hematology analyzer before and 60 minutes after blood donation. Data analysis began with the Shapiro–Wilk normality test and homogeneity test, which showed that the data were normally distributed and homogeneous (p > 0.05), followed by a Paired Sample t-Test. The results showed that the mean erythrocyte count decreased from 4.71 ± 0.49 million/µL before donation to 4.46 ± 0.51 million/µL at 60 minutes after donation. Statistical analysis indicated a significant difference between pre- and post-donation erythrocyte counts (p < 0.001). In conclusion, blood donation causes a significant decrease in erythrocyte count within the first hour after donation as an acute physiological response to blood loss. These findings highlight the importance of hematological monitoring and post-donation recovery education for donors.

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