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Dwi Anggorowati Rahayu
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Jurnal Riset Biologi dan Aplikasinya
Published by Universitas Negeri Surabaya
ISSN : -     EISSN : 26559927     DOI : 10.26740/jrba
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology
Jurnal Riset Biologi dan Aplikasinya is a peer-reviewed journal that strives to provide scientific information on the research results which focused on biological science (Ecology, Animal and Botanical Systematics, Microbiology, Zoology, Botany, Biotechnology, and Genetics and Evolution) and biological education (Innovation of Biology Learning, Assessment and Evaluation in Biology, and Media of Biology)
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 12 Documents
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Search results for , issue "Vol. 7 No. 1 (2025)" : 12 Documents clear
Optimal Dose Determination of Gamma Ray Irradiation of Alocasia suhirmaniana Yuzammi & A. Hay: Optimal Dose Determination of Gamma Ray Irradiation of Alocasia suhirmaniana Yuzammi & A. Hay Mulyani, Melza; A Husaini, Iin Pertiwi; Hariri, Muhammad Rifqi; Zulkarnaen, Rizmoon
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p31-37

Abstract

Alocasia suhirmaniana Yuzammi & A. Hay belongs to the Araceae family and has the potential for ornamental use. Nevertheless, this plant exhibits a limited leaf count and pliable leaf stems, necessitating the application of breeding techniques for its improvement. One important strategy empeloyed to improve the quality of ornamental plants is the breeding, which involves the use of gamma ray irradiation techniques. The objective of this study is to determine the developmental patterns and appropriate dosage levels of LD20 and LD50 Gamma ray irradiation in A. suhirmaniana callus. The development of callus, the quantity of shoots, and the establishment of rooted explants exhibit a stochastic pattern, with a decrease observed at a dosage of 5 Gy and an increase observed at 10 Gy. The LD20 and LD50 values for Gamma ray irradiation in A. suhirmaniana callus fall within the dose range of 7.14 Gy and 15 Gy, respectively. This dosage range is recommendedto achieve a greater diversity in genotypes and phenotypes. By successfully finding the optimal dosage of gamma ray irradiation, plant selection can be improved through enhancing genotype and phenotype characteristics.
The Effect of Domestic Waste on Mollusca Diversity and Their Role in the Ecosystem of the Mangrove Area in Tarakan, North Kalimantan Nugroho, Bimo Aji; Putri, Erisa Ayu Waspadi; Abrori, Fadhlan Muchlas
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p38-49

Abstract

Mangroves are vital ecosystems for biodiversity, but are increasingly threatened by pollution, especially domestic waste. This study focuses on the impact of domestic pollution on the diversity of Mollusca on Tarakan Island, North Kalimantan. Although several studies have examined molluscan diversity, there is a lack of analysis of the direct impact of pollution on these species. The method used was belt transects to measure species diversity, dominance and distribution of Mollusca in 4 habitats. The results were conducted in four mangrove habitats, and 36 species of Mollusca, 32 species of gastropods, and four species of Bivalvia were identified. The results showed that habitat 1, with the lowest level of pollution, had the most stable community index, while the habitat with the highest level of pollution showed a significant decline based on the community index. These findings confirm the importance of protecting mangrove ecosystems to maintain Bivalvia diversity and provide a basis for better environmental management policies. This research also opens opportunities for further studies on the interaction between pollution and biodiversity in mangrove ecosystems.
Protective Effects of Apple Peel Extract (Malus sylvestris Mill.) on the Sperm Quality and Testicular Histology in Mice Borax-Induced Atifah, Yusni; Adinda, Diva; Yuliati, Netri
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p20-30

Abstract

Borax, a toxic material, can disrupt the testes, hypothalamus, and spermatogenesis in the male reproductive system, leading to infertility. Apple peel contains quercetin, a substance crucial for boosting antioxidant levels and preventing free radical damage. The study aimed to assess the impact of apple peel extract on spermatozoa quality and testicular histology in borax-induced mice. The study utilized a Complete Randomized Design (CRD) with five treatment groups and five replications of each group: C- (without treatment), C+ (Borax), T1, T2, and T3, where borax was administered alongside various doses of apple peel extract: 0,2 mg, 0,4 mg, and 0,8 mg respectively. Testicular sections were collected for histopathological analysis and caudal epididymal sections for sperm quality assessment. Data were analyzed using ANOVA and DMRT tests using SPSS 23. The results showed that apple peel extract significantly increased the quality of mice spermatozoa in borax-induced mice (p<0.05). Apple peel extract to borax-induced mice also positively affected testicular diameter and weight, as well as the diameter of the seminiferous tubules and the number of Leydig cells. The optimal dose of apple peel extract in improving the quality of spermatozoa and testicular histology of mice was found to be0.4 mg / 20g BW.
DNA QR Code Using Internal Transcribed Spacer 1 (ITS1) Region of Commercial Cucumber Varieties (Cucumis sativus L.) Turhadi, Turhadi; Padmana, I Made Dhena Radya; Azrianingsih, Rodiyati
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p1-7

Abstract

DNA Quick Response (DQR) is an accurate, efficient, fast, and cost-effective alternative to PCR-based or other sequencing methods. DQR is the method integrated seamlessly with digital systems. The DQR could be apply as effective approach to preventing seed counterfeiting, for example in cucumber. This study aimed to identify DNA barcodes based on the ITS1 region and develop DQR for commercial cucumber varieties in Indonesia. Two cucumber varieties, namely cv. Mars and cv. Makka were used. The genomic DNA was extracted using commercial DNA purification kit. The DQR for these two cucumber varieties were developed based on experimental data from the ITS1 region. The efficiency and characterization of ITS1 region were evaluated through homology analysis using BLAST-NCBI and DQR development. The DQR of cucumber cv. Mars and cv. Makka based on ITS1 region were successfully developed. The length of the ITS1 region encrypted in the QR code was 496 bp and 437 bp for cv. Mars and cv. Makka, respectively. The A-T and G-C proportions for cv. Mars were 45.2% and 54.8%, respectively, while for cv. Makka, the A-T and G-C proportions were 42.6% and 57.4%, respectively. Our findings are valuable for genetic labeling and authentication of horticultural crops, especially cucumber.
Diversity of Functional Traits in Leaves of Syzygium Species in Lowland and Highland Simanjuntak, Susan; Winarni, Endang Widi; Rahman, Wiguna; Kurniawan, Vandra; Normasiwi, Suluh
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p8-19

Abstract

Syzygium is a genus of Myrtaceae with 1,236 species. The functional traits of each species vary. The purpose of this study was to determine the variation in the functional traits of Syzygium leaves found in the lowlands and highlands. In this study, Syzygium leaves were collected from the Bogor Botanical Garden (lowland) for 27 species, while from the Cibodas Botanical Garden (highland) for 35 species. Ten parameters were measured, including leaf Fresh Weight (FW), leaf Dry Weight (DW), Leaf Area s(LA), Specific Leaf Area (SLA), Leaf Dry Matter Content (LDMC), Specific Leaf Weight (SLW), Chlorophyll Content (CC), Stomatal Density (SD), Stomatal Length (SL), and Stomatal Width (SW). Based on the results of the study, a diversity of functional traits in Syzygium species was observed in the lowlands and highlands. The results also indicated that the elevation influences variations in functional traits. Most of the leaf traits were found to be higher in the lowland species. Phenotypic plasticity of parameters was observed within the species found in both lowland and highland locations
The Therapeutic Potential of Humic Acid in Streptozotocin-Induced Hyperglycemic White Rats (Rattus norvegicus L.) Sirait, Evi Marina; Rousdy, Diah Wulandari; Yanti, Ari Hepi
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p110-117

Abstract

Humic acid, an organic compound from peat soil, rich in antioxidants such as quinone and phenol, shows potential as an antidiabetic agent. This study aims to identify the optimal dose of humic acid extract for increasing body weight, lowering blood glucose levels, reducing malondialdehyde (MDA) levels, and improving the microanatomical structure of the pancreas in streptozotocin-induced diabetic rats. The humic acid was extracted from peat soil at Tanjungpura University. The study utilized 25 male Wistar rats (Rattus norvegicus), aged 8-12 weeks, organized in a group randomized design (GRD). Interventions included a single dose of streptozotocin (40 mg/kg b.w.) and varying doses of humic acid extract administered over 14 days post-induction. Results indicated that a dose of 375 mg/kg b.w. humic acid extract resulted in an increased final body weight by 11.86%, a reduction in fasting blood glucose levels by 79.23%, an AUC0-120 value of 19.311, and a final MDA level of 1.09 ±0.27 nmol/ml. These findings indicate the potential of humic acid as a therapeutic agent for diabetes.
Assessing Foliar Chlorophyll Content with SPAD-502 Chlorophyll Meter: A Comparison with Spectrophotometer Method in Various Plants Nurbaiti, Siti; Milasari, Asri Fajar; Wibowo, Wiko Arif; Nilamsari, Elvian Indah; Rachmawati, Diah
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p50-56

Abstract

Measuring chlorophyll content in plants is one of the main points that has never been ignored in various plant biology and agronomy research studies. Chlorophyll content is a parameter of growth and development, diagnosis of nutritional status, and response to environmental conditions. This study aimed to compare and determine the relationship between SPAD-502 chlorophyll meter readings and chlorophyll content obtained from acetone extraction followed by spectrophotometry measurement. Various leaves with different colors and thicknesses were used to determine the reliability of SPAD readings across diverse morphological traits. The results showed higher SPAD readings in leaves with a greener color. SPAD value was found to have a good linear relationship (r 0.8 and R2 0.64) and a positive correlation with total chlorophyll content, though variations due to leaf thickness suggest the need for correction factors. Furthermore, SPAD has potential as a rapid, non-destructive tool for monitoring plant health and nutrient status in agriculture, plant breeding, and horticulture. This tool can contribute to optimizing crop yield and managing fertilization practice, especially where the maintaining leaf integrity is essential for both commercial and aesthetic value. Species-specific calibration models are recommended to enhance measurement accuracy.
Diversity of Lactic Acid Bacteria in Senduro Goat Milk Trimulyono, Guntur; Salamun, Salamun; Fatimah, Fatimah
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p77-87

Abstract

Goat milk is currently widely consumed by Indonesian people. The Senduro breed of goats is one of the milk-producing goats widely cultivated by farmers in Senduro District, Lumajang Regency, East Java. Based on several studies, goat milk is a habitat for lactic acid bacteria, and research has been conducted on isolating lactic acid bacteria from goat milk.  This study aims to isolate lactic acid bacteria from Senduro goat milk samples characterized morphologically and biochemically. The pour plate method was used to isolate lactic acid bacteria, followed by the purification of isolates using the streak plate method. Colony characterization was carried out by observing the colonies' shape, edge, elevation, surface, and color. Gram staining, endospore staining, catalase test, and motility test were carried out to confirm lactic acid bacteria isolates. This study has successfully isolated 50 isolates of lactic acid bacteria that have Gram-positive, non-endospore, catalase-negative, and non-motile characteristics. The morphological characteristics of lactic acid bacteria isolate colonies from milk samples from the three farms showed diversity. Several isolates from the three farms showed similar characteristics. Further potential testing and identification of each lactic acid bacteria isolate is necessary to provide information regarding its roles.
Characterization and Molecular Identification of Lipolytic-bacterial Isolates Forming Biofilm on Polyethylene Plastic Ariyanti, Olivia Puja; Agustina, Putri; Anif, Sofyan; Seno, Hernandito Aryo; Hardianto, Alden Ganendra Madhava Priya; Mandiri, Listy Hasti; Zulperi, Dzarifah; Jamil, Nazia; Rahayu, Triastuti; Sidiq, Yasir
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p57-65

Abstract

Polyethylene (PE) plastic is one of the most widely used for multiple purposes and leading the environmental problems. Lipolytic bacteria are promising agents to reduce the plastic waste. The dry weight of PE plastic was reduced by 33 % in the Winogradsky's column after 45 days of incubation. However, the lipolytic bacteria responsible for those reduction was unknown. This study aimed to characterize and identify the potential lipolytic bacterial isolates forming biofilm on polyethylene PE plastics. Samples of lipolytic bacterial isolates were screened on tributyrin selective media based on the formed clear zone. Moreover, the 16S rRNA genes of the two most potential lipolytic bacterial isolates were amplified. Then, the amplicons of the 16S rRNA gene were sequenced. This study found two potential lipolytic bacteria isolates, AB A-2 and AB M-3, which had the characteristics of round colonies, wavy edges, convex surfaces, and milky white color. The two isolates are gram-positive and have the shape of Coccobacillus cells. The molecular identification showed that AB A-2 isolate was Bacillus sp., while AB M-3 isolate was Bacillus amyloliquefaciens. This finding contributes to novel bacterial isolates that potentially overcoming the plastic waste problem.
Somatic Embryo Enhancing of Phalaenopsis amabilis (L.) Blume Orchid with 6-Benzyl Amino Purine (BAP) Karend, Azzah Hanun Abas; Setiari, Nintya; Nurchayati, Yulita; Rashid, Fatimah Azzahra Ahmad
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p88-98

Abstract

Phalaenopsis amabilis (L.) Blume is an orchid with high economic value because of its beautiful white flowers, yellow labellum, and long stems. Its existence in nature is threatened with extinction, so efforts are needed to cultivate and preserve P. amabilis, one of which is through somatic embryos, which generally require Plant Growth Regulators (PGRs) in the form of 6-Benzyl Amino Purine (BAP). 6-Benzyl Amino Purine (BAP) is used because it is one of the cytokinin hormones proven to induce somatic embryos. This study aims to examine and determine the use of the right BAP concentration to propagate P. amabilis through somatic embryo induction. The explants used were P. amabilis protocorms; because protocorms produced a greater number of somatic embryos than leaf explants, the development of the explants was observed every week for 2 months. This study used a single factor Completely Randomized Design (CRD) in the form of BAP concentrations of 0, 1, 2, 3, and 4 ppm with 20 replications. The results showed that the most optimal treatment for the propagation of P. amabilis somatic embryos was on media with an average number of somatic embryos at a concentration of BAP 3 ppm (65.00) embryo/explain.

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