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3BIO: Journal of Biological Science, Technology and Management
Published by Institut Teknologi Bandung
ISSN : -     EISSN : 26558777     DOI : http://dx.doi.org/10.5614%2F3bio.2020.2.1
Core Subject : Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Immunology & microbiology Neuroscience
3Bio: Journal of Biological Science, Technology and Management is an interdisciplinary peer-reviewed journal in a wide aspect related to the field of life sciences and other related fields of study. The journal aims to promote scientific discourse and disseminate research on various branches and applications of bio-science, biotechnology and bio-based management. This journal invites original empirical research, literature reviews, theoretical or methodological contributions, or short communications on, but not limited to, the following topics: - Ecology and Biosystematics - Microbiology - Genetics and Molecular Biology - Animal Development and Physiology - Plant Development and Physiology - Entomology - Biomedical science - Biochemistry - Agronomy - Forestry - Bioengineering - Bioethics - Management of Biological Resources The journal also invites contributions from other associated disciplines. This journal is an open-access journal. Readers may read, download, copy, distribute, print, search, or link to the full texts of these articles without any charge, provided that readers acknowledge the Creative Commons attached to the articles. All submitted papers are reviewed by at least two referees before being accepted for publication, following a double-blind review process. This journal is delivered in an academic English and rigorously edited to provide a quality standard of a scientific journal. This journal is managed by the School of Life Sciences and Technology, Institut Teknologi Bandung, Indonesia.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 74 Documents
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Effectiveness of Tea Leaf (Camellia sinensis) Liquid Smoke as an Antiseptic Rohmah, Fitriani Nurhidayati; Alamsyah, Muhamad Thamrin; Nurjana, Siti; Fadhila, Feldha; Mayuri, Nindya Sekar; Rumidatul, Alfi
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 1 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.1.1

Abstract

Microbes are often the cause of infectious diseases; one method of prevention is to use antiseptics. Excessive use of antisep- tics can cause mild to severe side effects, so an alternative antiseptic is needed. Liquid smoke is an alternative raw material to produce antiseptics because its main component can be used as an inhibitor of microbial growth. This study aims to determine the effectiveness of tea-leaf liquid smoke as an antiseptic against microbial growth in vivo and in vitro. The method for producing liquid smoke via pyrolysis involves distilling grade 3 liquid smoke to generate grade 1 and grade 2 liquid smoke. The in vitro test uses a microbial growth inhibition test with grade 1 and 2 liquid smoke concentrations of 35%, 50%, and 75%. The microbes used were Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Candida albicans ATCC 10231, and Aspergillus flavus ATCC 9643. The in vivo test uses the swab test method on the palm of the hand. The results of the invitro test showed that the largest inhibition zone of tea leaf liquid smoke was obtained using grade 2 liquid smoke with a concentration of 75%.The results of the in vivo test showed that the effectiveness of tea leaf liquid smoke was 80% against bacteria and 85% against fungi. Based onthe results of the study, it can be concluded that tea leaf liquid smoke (Camellia sinensis) has potential as an antiseptic.
The Effect of Photoperiod on the Growth of Stevia rebaudiana in vitro Ramadhan, Muhammad Syah; Esyanti, Rizkita Rachmi; Iriawati, Iriawati; Rahmawati, Andira
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 1 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.1.2

Abstract

Stevia rebaudiana, the source of non-caloric natural sweeteners in the form of steviol glycosides, is a plant with a poor germi- nation rate.Therefore, micropropagation is a potential alternative method to propagate the plants in a large number. Light is an important factor for photosynthesis, so changing the intensity, quality, and duration of lighting can affect plant growth. Photo- period, the duration of light within 24hour period, has been known to influence the growth of S. rebaudiana grown in ex vitro conditions. The purpose of this study is to investigate the effect of various photoperiod (8, 12, and 16 hours) on the growth of S. rebaudiana in vitro. The node segments from ex vitro grown S. rebaudiana plants were cultured on solid MS media supple- mented with 1.13mg/L BA and 0.35 mg/L IAA. The shoots were rooted on solid half-strength MS media containing 0.1 mg/L IAA. For acclimatization, therooted shoots were grown on a mixture of fertile soil, burnt rice husk, cocopeat, and manure. The photoperiod treatment was applied from the beginning to the end of the experiment. Our results showed that the highest shoot length was found under 16 hour photoperiod. On the otherhand, root number and root length were not affected by photoperiod. Additionally, a 16 hour photoperiod increased shoot length (5.9 cm) compared to a 12-hour (3.48 cm) and 8-hour photoperiod (3.08 cm) after 5 weeks of acclimatization. A 16 hour photoperiod also producedhighest total leaf fresh weight (0.2 g). Howev- er, different photoperiods did not significantly affect leaf number and leaf area. In conclusion, 16 hour photoperiod is the best condition for S. rebaudiana micropropagation.
Business Model Canvas (BMC) Approach for Ecotourism Development Based on Islamic Boarding School Community (Case: Cipeujeuh Valley, Darul Arqam Muhammadiyah Islamic Boarding School) Al-Sakina, Nashiha; Yustiana, Yooce; Furqan, Alhilal
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 2 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.2.1

Abstract

This study investigates the potential for ecotourism in Cipeujeuh Valley, which is situated near the Darul Arqam Muhammadiyah Islamic boarding school in Garut Regency. The research examines the factors that drive tourists to visit the area, the level of community engagement, and the development of a community-based ecotourism business model. The data was gathered using descriptive statistics, the Ecotourism Opportunity Spectrum (ECOS) framework, and rapid rural appraisal techniques. The results indicate that Cipeujeuh Valley has intermediate ecotourism spectrum, with push factors such as sports and adventure, recreation and relaxation, social status, and quality time, and pull factors including nature and the local community, and enjoying facilities. The community is generally receptive to ecotourism development, with 61.3% expressing interest and 38.7% not. The study suggests that educational institutions, communities, organizations, and families can be targeted through a business model canvas that offers unique environments, sustainable resource use, community empowerment, educational tourism, and economic development. Key resources include natural attractions, religious and local wisdom activities, infrastructure, and a skilled workforce, while key activities involve nature-based and community-based tourism experiences.  
Application of Compost Tea and Ascorbic Acid to Increase Productivity and Antioxidant Activity of The Common Bean (Phaseolus vulgaris L.) Nasution, Ahmad Raihan; Dwiartama, Angga; Putra, Ramadhani Eka
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 2 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.2.4

Abstract

The common bean is a functional food rich in nutrients and bioactive compounds that support good health. Improving common bean yield is essential for enhancing nutrition within communities. This study examined the effects of compost tea, ascorbic acid, and their combination on the productivity and antioxidant activity of upright common beans. The experiment involved six treatments, including a control (water spraying) (P1), ascorbic acid (P2), manure compost tea (P3), BSF larvae production residue (BSFR) compost tea (P4), manure compost tea and ascorbic acid (P5), and BSFR compost tea and ascorbic acid (P6). All treatments, except P6, showed significant differences in productivity. P2 recorded the highest yield at 8,13 tons/ha/cycle and increased productivity by 19,03%, followed by P5 (9,22%) and P3 (3,95%) compared to P1 (control). P4 had a slight reduction in productivity by 3,95% than P1. A combination of compost tea and ascorbic acid increased productivity by 5,07% for P5 and 4,63% for P6 compared to the treatment without combination with ascorbic acid. P2 had the lowest IC50 value, 17,46 µg/ml, indicating the highest antioxidant activity. Compost tea and ascorbic acid combined reduced the IC50 value by 23,12% for P5, but P6 increased it by 32,59%. P5 improved the antioxidant activity of common bean pods when combined with compost tea and ascorbic acid, while P6 demonstrated a better effect without the combination. P3 and P6 had higher IC50 values than the control but lacked the potential to improve the antioxidant activity of common bean pods.
Biomonitoring of Air Quality Using Lichen as Bioindicator in The Greater Bandung Area, West Java Saragih, Dora Erawati; Irwanto, Rina Ratnasih; Taufikurahman, Taufik
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 2 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.2.3

Abstract

Air quality assessment in an area can be determined by conducting biomonitoring using bioindicator organisms, including lichen. Lichen is a symbiotic organism that passively absorbs nutrients and chemical compounds from the air. This research was conducted in the Greater Bandung area, including Bandung City and West Bandung Regency, to observe the abundance of lichen species so that the purity of the atmosphere could be evaluated at each research location and determine the effect of environmental variables on lichen abundance.The survey area was divided into 40 units spread over two locations in Bandung, namely Djuanda street (JD), Kebon Kawung street (KK), and two locations in West Bandung Regency, namely Padalarang street (PD) and Curug Cimahi (CC). CC locations with low levels of pollution were used as comparison areas. Lichen samples from observation locations were identified, and the number of colonies, frequency of closure, and diversity (H’) were counted so that the Index of atmospheric purity (IAP) value at each observation location could be known. As many as 24 species from 14 lichen families with a total of 256 colonies were found in the four observation sites with the percentage of thallus crustose (62%), foliose (37%), and fruticose (1%). The highest lichen diversity was found in the CC area (2.62), followed by JD (1.99), PD (1.63), and the lowest in KK (0.90). The lowest IAP result was in KK (10.21), followed by PD (17.70) and JD (31.85). The location with the highest IAP was obtained at CC (46.65), indicating that the environmental conditions were still good, while other locations were polluted.
The Influence of Polyethylene Glycol Precipitation Methods on Yield and Purity of White Radish Peroxidase Faizah, Nur Al; Giri-Rachman, Ernawati Arifin; Niloperbowo, Wardono
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 2 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.2.5

Abstract

Proteins are widely used in various industries as highly valued biotechnology products. One example is horseradish peroxidase isolated from horseradish (Armoracia rusticana) that used as enzyme label in immunochemistry. However, the cultivation of horseradish is limited to subtropical countries, making the dependency on horseradish peroxidase unsustainable for tropical countries. Numerous studies have explored alternative peroxidases, and white radish peroxidase isolated from Raphanus sativus L. has emerged as a promising candidate. In this study, white radish peroxidase is isolated using the polyethylene glycol (PEG) precipitation method which is widely used as a simple and cost-effective method. This study aims to evaluate the effectiveness of the one-step and two-step PEG precipitation method. The one-step PEG precipitation method used in this study was done by mixing the white radish juice with PEG 6000 30% (w/v), while the two-step method was done by mixing it with PEG 400 20% (w/v) and PEG 6000 30% (w/v) consecutively. This study compares the yield and recovery levels of total protein and white radish peroxidase, as well as the enzymatic specific activity of white radish peroxidase isolated both by the one-step PEG precipitation and the two-step PEG precipitation. The results indicate that both extraction methods yield the same level of white radish peroxidase. However, they differ in terms of purity. The two-step extraction method results in white radish peroxidase with higher purity, as evidenced by its specific activity towards the chromogen ABTS in the presence of H2O2.
Enhancing Lipid Extraction from Chlorella vulgaris Microalgae for Biodiesel Production: Application of Natural Deep Eutectic Solvent (NaDES) in Cell Disruption Priharto, Neil; Nadia Yasmin Dicky
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 2 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.2.2

Abstract

Natural deep eutectic solvent (NaDES) pre-treatment offers a promising alternative strategy to enhance lipid extraction efficiency from microalgae by influencing the integrity of the cell wall, thereby improving solvent accessibility to the cytoplasm and facilitating the release of intracellular lipid content. In this study, Chlorella vulgaris biomass underwent pre-treatment with four different NaDES formulations, each based on choline chloride (ChCl) with varying molar ratios: ChCl : Glycerol (1:2), ChCl : Glycerol (1:1), ChCl : Oxalic Acid (1:2), dan ChCl : Oxalic Acid (1:1).We analyzed the impact of these NaDES pre-treatments on lipid yield and fatty acid profiles. The extracted lipids exhibited an acid value of 42.56 mg KOH/g fat and a free fatty acid content of 0.25%. Samples subjected to NaDES treatment showed significant increases in lipid extraction efficiency, with lipid yields ranging from 1.25 to 2.3 times higher than those of untreated biomass extracted using hexane (p < 0.05). The highest lipid yield was observed in samples treated with ChCl : Glycerol (1:1), achieving a total lipid yield of 19.44% (w/w), more than double that of the untreated biomass. Although minor variations in fatty acid profiles were noted due to the NaDES treatment, the dominant fatty acids in each variation remained palmitic acid (C16:0, 21-29.5%) and oleic acid (C18:1, 13-43.88%).
Effectiveness and Public Perception of Synthetic and Natural-Based Mosquito Repellents Against Aedes aegypti in Indonesia Ahmad, Intan; Amalia, Riska; Yusmalinar, Sri
3BIO: Journal of Biological Science, Technology and Management Vol. 7 No. 1 (2025): Vol. 7 No. 1 (2025)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2025.7.1.1

Abstract

Since 1968, dengue fever cases in Indonesia have persisted despite various vector control efforts. One of the most common and accessible methods of personal protection is the use of mosquito repellents. This study evaluated public perceptions of mosquitoes and repellents, their willingness to pay (WTP) for these products, and the efficacy of synthetic and natural-based repellents against Aedes aegypti, considering variations in mosquito strain and age. A Knowledge, Attitude, and Practices (KAP) analysis was conducted to assess public understanding and behaviors, utilizing the Analytical Hierarchy Process (AHP) and SWOT-AHP. Repellent efficacy was tested using the arm-in-cage method based on WHO guidelines, involving four mosquito strains (BORA, BDG, TSK, TGR) and three age groups (5, 10, 15 days). The KAP analysis revealed high public awareness but limited translation into effective practices due to differing perceptions and low personal responsibility for dengue prevention. Natural-based repellents were preferred, scoring 3.40 out of 5, with WTP ranging from IDR 20,000–40,000. However, only synthetic repellents containing DEET provided 90% protection for six hours, while natural-based repellents offered less than 60% protection in the first hour. Strain variation significantly affected repellency, with TSK showing the highest repellency, while BORA and BDG exhibited similar trends. Although 10-day-old mosquitoes were more sensitive to repellents, age variation did not consistently influence repellency. These findings highlight the need for improved education campaigns, tailored repellent formulations, and localized testing to enhance public protection against mosquito bites and dengue transmission.
Analysis of Quercetin from Allium cepa L. Skin Waste in Inhibiting α-Amylase: An Alternate Therapeutic Drug to Treat Diabetes Muthahhari, Muhammad Zaky; Qordhowi, Muhammad Hisyam; Bonardi, Mohammad Rafi; Sofiani, Tania; Simanjuntak, Samuel Balapradana; Mustari, Eri
3BIO: Journal of Biological Science, Technology and Management Vol. 7 No. 1 (2025): Vol. 7 No. 1 (2025)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2025.7.1.3

Abstract

Type 2 diabetes is a chronic metabolic disease characterized by an increase in blood sugar due to insulin resistance as well as the decline of insulin production by the pancreas. Quercetin is a flavonoid secondary metabolite that could be utilized as an α-amylase inhibitor and can potentially be used as a therapeutic drug in type 2 diabetes treatment by inhibiting starch hydrolysis in the human body. This research aims to determine the quercetin yield of Allium cepa L. skin waste, evaluate the α-amylase inhibitory properties of the extracted quercetin, and delve into the molecular mechanism of α-amylase inhibition by quercetin. Methods that were used in this research consisted of sample collection and preparation, quercetin extraction, testing for quercetin content in extract by spectrophotometry and HPLC, as well as testing for α-amylase inhibition by quercetin. Quercetin extraction produced 1.52 g of dry extract, yielding 16.8 mg of extract per g of dry shallot skin. Spectrophotometric analysis yielded 3.37 mg of quercetin per gram of dried shallot skin, while HPLC yielded 3.99 mg/g. The shallot skin extract inhibited α-amylase with an IC50 of 348.2 µg/mL. In comparison, acarbose had a more potent inhibitory effect, with an IC50 of 237.4 µg/mL. Quercetin inhibits α-amylase by forming hydrogen bonds with the active sites of the enzyme (Asp197, Glu233, and Asp300).
Melon Waste Ecoenzyme: An Eco-friendly Liquid Organic Fertilizer for Vegetative Growth of Melon (Cucumis melo L. var. Merlin) Ulmillah, Aulia; Satitiningrum, Yuni; Agilliana, Intan
3BIO: Journal of Biological Science, Technology and Management Vol. 7 No. 1 (2025): Vol. 7 No. 1 (2025)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2025.7.1.2

Abstract

The utilization of melon plant waste, such as fruit peels, leaves, and stems, can be done by processing it into eco-enzyme, which is then used as liquid organic fertilizer. This study aims to assess the effect of eco-enzyme on the vegetative growth of melon plants. The randomized block design (RBD) method was used with seven treatments and four replications, including P0 (negative control), P1 (10 mL/L), P2 (20 mL/L), P3 (30 mL/L), P4 (40 mL/L), P5 (50 mL/L), and P6 (positive control). Observations were made every 7 days for 30 days, with parameters including plant height, leaf area, number of leaves, plant fresh weight, and root fresh weight. Data analysis was conducted using one-way ANOVA and Duncan’s test. The results showed that treatment P5 (50 mL/L) produced the best results with a plant height of 36.68 cm, leaf area of 137.33 cm², 11.56 leaves, plant fresh weight of 67.1 g, and root fresh weight of 2.24 g. These results were not significantly different from the positive control (P6). Thus, eco-enzyme had a significant effect on the vegetative growth of melon plants.

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