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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
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Articles 15 Documents
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Search results for , issue "Vol. 29 No. 3 (2022): May 2022" : 15 Documents clear
Allometric Model, Aboveground Biomass and Carbon Sequestration of Natural Regeneration of Avicennia lanata (Ridley). at in-active Pond of Muna Regency, Southeast Sulawesi Fajar Hasidu; Arif Prasetya; Maharani Maharani; Muhammad Syaiful; Kangkuso Analuddin
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.399-408

Abstract

This study aimed to establish an allometric model for estimation of aboveground biomass, and carbon sequestration in A. lanata mangrove forest growing in Muna Regency, Southeast Sulawesi. Research methods were done by transect and 5 quadrats with size of 100 m2 each. A total of thirteen individual trees with different sizes were harvested. While DBH and D30 were measured. The samples were separated into stems, branches, and leaves and then weighted. The sample from each fresh organs were taken and brought to the Laboratory and then oven dried at 80°C for 7 days. The allometric equations were established by using independent variables (DBH and D30), and dependent variables (Ws, Wb, Wl). The partial and overall aboveground biomasses were calculated from allometric model, while carbon stock and CO2 sequestration were estimated. The results showed that the independent variable of DBH was more applicable for estimation of Ws, Wb, Wl, and total biomasses (Mg ha-1) of A. lanata forest, which were estimated as 28.28±3.48, 6.40±0.79, 5.00±0.66, and 40.08±4.97 respectively. The carbon stock in stems (13.24±1.63 Mg ha-1) was higher than in branches (3.01±0.37 Mg C ha-1) as well as in leaves (2.35±0.31 Mg C ha-1). The total of carbon stock were estimated at about 18.83±2.33 Mg C ha-1. Meanwhile, the total of CO2 absorption by A. lanata mangrove was 43.95±5.45 Mg CO2 ha-1. Therefore a regenerated A. lanata mangrove in this in-active pond area had potentiality on carbon stock and sequestrations, although these vegetation condition was still in the growth stage.
The Banana (Musa balbisiana Colla) Peels Flour Modulates HTR2B Receptor Expression in the Liver Diabetic Rats Andreanyta Meliala; Paramita Narwidina; Herlin Ajeng Nurrahma; Irwan Supriyanto
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.279-287

Abstract

Uncontrolled diabetes mellitus (DM) has been linked to depression and lipid metabolism impairment. Serotonin (5-HT) has been known to improve lipid metabolism and affect mood. The 5-Hydroxytryptophan (5-HTP) in banana peel flour (BPF) is a precursor to synthesize 5-HT in the body, which is linked to initiating liver regeneration via HTR2B receptors. The impact of 5-HTP in the diet on HTR2B receptor expression has yet to be thoroughly investigated. We aimed to elucidate the correlation between serum lipid profile and immobility time in Tail Suspension Test (TST) as depressive-like behavior and compare the expression of HTR2B receptor in healthy and diabetic rats. Male Wistar (Rattus norvegicus) rats were assigned to the control and treatment groups in a DM model with streptozotocin (60 mg/kg) injected intraperitoneally, then either fed standard diet or BPF 10% supplemented standard diet for 21 days. Immunohistochemical staining was used to examine the expression of 5-HT, HTR2B receptors. We determined that diabetic rats fed the standard diet supplemented 10% BPF group showed significantly lower concentrations of total cholesterol and triglycerides compared to diabetic rats fed a standard diet and it was positively correlated between total cholesterol and LDL with the duration of immobility time in TST. The average immunoreactivity score in diabetic rats fed 10% BPF was the highest among the other groups, indicating that the available BPF dose is sufficient for HTR2B activation, which will support the liver cell regeneration process, and should be investigated further.
The Potential Roles of Gut Microbiome in Modulating the Immune Response of Asian Redtail Catfish (Hemibagrus nemurus) Vaccinated with Aeromonas hydrophila Septyan Andriyanto; Yani Aryati; Tuti Sumiati; Angela Mariana Lusiastuti; Nurhidayat; Kurniawan Kurniawan; Rofingah Kuswatun; Nining Nuraeni
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.266-278

Abstract

The research aims are to observe the effect of vaccination in microbial profiles and gut microbiome composition. The treatments were as follows: the fishes were injected with PBS and challenged (A); the fishes were injected with freeze–dried vaccine dissolved in 100 ml 0.85% NaCl and challenged (B); the fishes were injected with freeze–dried vaccine dissolved in 50 ml 0.85% NaCl and challenged (C), and the fishes were injected with liquid vaccine and challenged (D). Microbiome composition measurements were carried out on the 21st–day post–vaccination and the 7th day after the challenge test. Fish intestine samples from three replications were tested by Next Generation Sequencing (NGS). Two significant phyla were identified from all treatments, namely Proteobacteria and Firmicutes. Cetobacterium, Candidatus Bacilloplasma, and Clostridium sensu stricto were the genera classified as good bacteria in vaccinated fish. It can be concluded that vaccination can increase the diversity of the gut microbiome, especially bacteria beneficial to the fish host. Chitosan as a coating antigen in freeze–dried vaccine increases gut microbiome's number and diversity better than a liquid vaccine.
Cloning, Expression, and In Silico Analysis of Class IV Poly-(R)-3-hydroxybutyrate Genes from New Strain of Bacillus thuringiensis TH-01 Zuhdina Sabiqoh; Rukman Hertadi; Enny Ratnaningsih
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.310-319

Abstract

Poly-(R)-3-hydroxybutyrate (PHB) is a bioplastic derivative of polyhydroxyalkanoate (PHA) which can be synthesized by bacteria under certain growth conditions. Previous study has reported a new strain of Bacillus thuringiensis TH-01 isolated from thermite, which found to accumulate PHB. This research aimed to clone PHB biosynthesis genes from B. thuringiensis TH-01 and study its expression as well as predict the tertiary structure of the enzymes. The clone of phaA gene, which encodes PhaA, was obtained as 1182 bp. On the other hand, 2546 bp clone of phaRBC gene cluster was obtained to consist of 744 bp phaB, 1086 bp phaC, and 483 bp phaR, encoding respective PhaB, PhaC, and PhaR proteins. In silico analysis indicated that PhaA, PhaB, PhaC, and PhaR, revealed to have 393, 247, 361, and 160 amino acid, respectively. The predicted model of PhaA, PhaB, and PhaC showed dominant structure of α/β folding motif, while PhaR was dominated by a helix-loop-helix motif. The catalytic residues of PhaA were Cys88, His349, and Cys379, whereas the catalytic residues of PhaB were Ser142, Tyr155, and Lys159. These catalytic residues were identical to those residues obtained in other PHB biosynthetic enzymes reported elsewhere, confirming that our clones were of PHB biosynthetic genes.
The Inhibition of 15-Lipoxygenase by Blechnum orientale Leaves and its Glycoside-flavonoid Isolates: In Vitro and In Silico Studies Rissyelly Rissyelly; Syaikhul Aziz; Frangky Sangande; Agung Wibawa Mahatva Yodha; Krisyanti Budipramana; Elfahmi Elfahmi; Sukrasno Sukrasno
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.353-359

Abstract

Fern is one of the groups of primitive plants rich in secondary metabolites that are commonly used to treat various diseases, including antioxidant, anti-hyaluronidase, anti-inflammation, and respiratory disease but less investigated. Flavonoid is one of the secondary metabolites abundantly present in ferns. This study aims to isolate major compounds found in Blechnum orientale act as 15-lipoxygenase (15-LOX) inhibitors. Inhibition of lipoxygenase decrease the production of leukotriene that induces bronchoconstriction in asthma. Isoquercitrin (Quercetin-3-O-β-glucopyranoside) and trifolin (kaempferol-3-O-β-D-galactoside) have been successfully isolated from Blechnum orientale. Further in silico study was performed to explain the binding mode between flavonoid pyranoside or galactoside and flavonoid aglycone in the 15-LOX cavity and their amino acid residues interaction. Isoquercitrin binds with Ile663, Ile400, Leu408, Leu597, Ala404, and Arg403 in the 15-LOX cavity as a lipoxygenase inhibitor. Trifolin binds the same amino acids as isoquercetin with addition His366, Gln596, and Phe175. Both isoquercitrin and trifolin act as competitive inhibitors against lipoxygenase enzymes.
Cross-Species Amplification and Variability of Microsatellite DNA Markers in Domesticated Indonesian Mahseer; A Case Study with Tor soro, Tor douronensis and their Interspecific Hybrids Imron Imron; Agung Asrori; Khotibul Umam; Otong Zenal Arifin; Dessy Nurul Astuti
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.409-416

Abstract

Indonesian mahseer (Tor spp.) are freshwater species of high economic, cultural, and conservatory values. Owing to their high values and environmental degradation, the population of Tor fish gradually decreased, and domestication efforts have been made to conserve the population. This study was aimed to assess the cross-species amplification and microsatellite genetic diversity in Indonesian mahseer Tor soro (SS), Tor douronensis (DD), and their interspecific hybrids using primers developed for Tor putitora. Eleven primer sets were used to test for amplifiability and screen genetic diversity in 40 progenies derived from those three groups. Results showed that seven primer sets (64%) successfully amplified loci. Genetic screening using the three most consistently amplifying primers showed that the number of alleles in the three populations was low, ranging from 2 to 5 alleles. The observed heterozygosity (HO) was high ranging from 0.650 to 0.789, and the fixation index (FIS) was negative, indicating heterozygosity excess. In line with other parameters, the P-values of the HW parameter of several loci-population combinations were significantly departed from equilibrium (P <0.05). A few private alleles were observed in parental line DD and the hybrids. Overall, the cross-species primers developed from T. putitora were able to amplify loci in T. soro, T. douronensis and their hybrids and genetic diversity in the hybrid population was slightly higher than those in parental lines. Possible factors driving the phenomena and practical implications of these findings on the conservation measures are discussed.
Alkaline Phosphatase Activity of Plant Growth-Promoting Actinomycetes and Their Genetic Diversity Based on the phoD Gene Muhammad Faiz Amri; Edi Husen; Aris Tjahjoleksono; Aris Tri Wahyudi
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.360-369

Abstract

Actinomycete is one of the beneficial bacteria groups inhabiting rhizosphere soil. They can promote plant growth through various mechanisms. In the previous study we have isolated rhizosphere actinomycetes from maize rhizosphere with direct plant growth promotion characters. The aims of the present study were to analyze the ability of maize rhizosphere actinomycetes to solubilize phosphate, determine alkaline phosphatase activity, and study their genetic diversity based on phoD gene. Thirteen rhizosphere actinomycete isolates were able to solubilize phosphate at concertation range 55.84±2.27 mg/L to 144.48±5.71 mg/L. The activity of extracellular alkaline phosphatase was exhibited by all maize rhizosphere actinomycetes isolates in various level ranging from 0.08 mU/mL to 0.51 mU/mL. The phoD gene, one of the three homologous genes which encode alkaline phosphatases, was successfully detected in all isolates and identified as alkaline phosphatase D of Streptomyces spp. The partial phoD sequences of the isolates were located within metallophosphatase domain of alkaline phosphatase D. Alignment analysis showed that the deduced amino acid sequences of PhoD were mostly conserved in the isolates and Streptomyces spp. Essential residues involved in the active core arrangement of PhoD which binds metal ion cofactors were conserved. Constructed phylogenetic tree showed that the isolates were divided into two groups within PhoD cluster. PhoD of the isolates and Streptomyces spp. had closer relationship to purple acid phosphatase compared to other homologous PhoA and PhoX which form separate cluster. Generated three-dimensional structure model of partial PhoD had high similarity to alkaline phosphatase D of Bacillus subtilis (2YEQ) and showed overlapping structure based on super-positioning analysis.
DNA Barcoding of Neolissochilus sumatranus and Tor douronensis to Support In Situ Conservation of Indonesian Mahseers Sekar Larashati; Novi Mayasari; Yuli S Fitriana; Gema Wahyudewantoro; Mey R Widoretno; Tri Widiyanto
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.370-377

Abstract

The fishes of genera Neolissochilus and Tor are locally known as Ikan Batak or ihan in North Sumatra. They are sacred and are usually served in Batak tribe traditional ceremonies. However, their population in the wild is declining because of habitat degradation, unwise exploitation, water pollution, and the introduction of invasive species. Therefore, suitable management is needed to maintain Ikan Batak's sustainability in their natural habitat, which can be conducted through in situ conservation. The habitat of Ikan Batak in the Bonan Dolok river of the Samosir Regency can potentially be developed as a conservation site. Information concerning this species in Bonan Dolok River is needed as a basis for effective in situ conservation management. Morphologically identified fish captured in Bonan Dolok River were validated using DNA barcoding of cytochrome oxidase I (COI) mitochondrial DNA segment. This study confirmed that Ikan Batak captured from Bonan Dolok River belongs to two genera, Neolissochilus and Tor. Furthermore, the phylogenetic tree construction using Maximum Likelihood showed that the specimens identified as N. sumatranus were identical to N. cf soroides, a species found in the Malaysian peninsula, Thailand, and Cambodia.
Biocontrol Activity, Mode of Action, and Colonization of Aureobasidium pullulans Dmg 30 DEP on Controlling Early Blight Disease on Tomato Plant Wawan Setiawan; Suryo Wiyono; Toga Pangihotan Napitupulu; Atit Kanti; Idris Idris; Masrukhin Masrukhin; Efi Toding Tondok; I Nyoman Sumerta; I Made Sudiana
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.320-329

Abstract

We investigated the biocontrol activity of A. pullulans Dmg 30 DEP against Alternaria solani causal agent of early blight. Biocontrol activity was tested by the in vivo and ad planta. Biocontrol activity were tested by investigating the antibiosis capabilities with dual culture method, paper dish assay, two-compartment petri dish assay, and trapping and identification of volatile organic compounds (VOCs) with GC-MS. Lysis activity was examined by observing the clear zone formed by growing yeast on chitin agar and skim milk agar. The ability of hyperparasitism was assessed by the agar block method, and observed by light microscopy and scanning electron microscopy (SEM). The results showed that A. pullulans Dmg 30 DEP plays a role in the suppression of early blight disease at 106 cells/ml and 107 cells/ml yeast cell density. The mechanism involved in biocontrol activity is the production of VOCs, the production of chitinase and protease enzymes, the production of siderophore and hyperparasitism. The result shows that A. pullulans Dmg 30 DEP was colonizing the tomato leaves following the areole.
Potential of Conditioned Medium of hATMSCs in Aging Cells Model Wahyu Widowati; Rachmawati Noverina; Wireni Ayuningtyas; Dedy Kurniawan; Seila Arumwardana; Hanna Sari W Kusuma; Rizal Rizal; Dian Ratih Laksmitawati; Ratih Rinendyaputri; Rilianawati Rilianawati; Ahmad Faried
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.378-388

Abstract

Skin aging is caused by the exposure cumulative of ultraviolet radiation, it leads reactive oxygen species (ROS) production in the skin. The conditioned medium of human Adipose Tissue-derived Mesenchymal Stem Cells (hATMSCs) can scavenge free radicals and increase the survival rate of skin cells under oxidative stress. This study examined the protective effects of Conditioned Medium (CM) of hATMSCs in H2O2-induced human skin fibroblast cell line (BJ). The aging cells model using H2O2-induced BJ cells were added CM-hATMSCs in concentrations (0, 10, 30%) and incubated in various time, furthermore BJ cells induced by various H2O2 concentrations (0, 50, 100, 200 µM) incubated for 1 h. The anti-aging potential were measured including viability, ROS and collagen levels in BJ cells which treated CM-hATMSCs. The median inhibitory concentration (IC50) of H2O2 on BJ cells for 1 h incubation was 107.87 μM and 91.25 μM for 10 min incubation. CM-hATMSCs increased the viability on aging model cells. CM-hATMSCs concentration 30% increased the viability of H2O2 50, 100, 200 µM-induced BJ cells. CM-hATMSCs concentration 25% decreased ROS, increased collagen level in H2O2 50, 100, 200 µM-induced BJ cells. CM-hATMSCs increase the viability cells, collagen level and decrease ROS level in aging model cells.

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