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Nusantara Science and Technology Proceedings
Published by Future Science
ISSN : -     EISSN : 26229692     DOI : -
NST Proceeding supports regional research communities to globalise their findings in Science and Technology by providing an open access, online platform in line with international publishing standards and indexing scholarly conference proceedings. The current emphasis of the NST Proceeding includes (but is not limited to) the following areas: Life Science, Mathematics, Eductation, Social Science, Medicinal Science and etc. All conference papers published on the NST Proceeding are fully Open Access. Open Access publications are freely and permanently available online to any reader, anywhere in the world without subscription to the publications in which these articles are published. Unrestricted use, distribution, and reproduction in any medium are permitted, provided the author/editor is properly attributed. NST Proceeding will provide high-quality peer review by scientific comittee and proofreading service by native speaker to make sure the language quality. We are the best in rapid publication processes for the open access content, maximum visibility and all-time availability for the published articles, citation tracking and indexing in a variety of databases.
Articles 1,542 Documents
Cell Line Authentication (CLA) using Short Tandem Repeat (STR) Method on Breast Cancer cell line MCF-7 Pendrianto; Muhammad Samsul Mustofa; Silviatun Nihayah
Nusantara Science and Technology Proceedings Multi-Conference Proceeding Series B
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Abstract

Research on human diseases, especially in cancer begins with in vitro studies using cell lines. However, cell lines grown in vitro can occur cross-contamination or even miss-identification. This requires a cell line authentication (CLA) procedure using a molecular approach. In this process, analysis of allele variance from several loci is needed to ensure that this variant matches the expected allele. The STR method can be used to authenticate cell lines, because it can compare allele profiles with a sample of known standard cell lines, and also able to provide simple, inexpensive, and very specific genetic "fingerprints" on cell lines. It is necessary to authenticate cell lines in collections of cell lines in the Central Laboratory for Stem Cell Studies at the YARSI University Research Institute, so that in vitro and in vivo studies on anticancer, antioxidants, aging, and longevity involving cell lines will produce valid outcomes in future research. The research method was laboratory experimental. breast cancer cell lines MCF-7, grown on growth media containing serum and antimicrobial-antimycotic. Genomic DNA was isolated by Maxwell® RSC Whole Blood DNA Kit. PCR process using GlobalFiler™ PCR amplification Kit (Applied Biosystem) and fragment analysis was analyzed with Genetic Analyzer 3500. The preliminary results of fragment analysis on two MCF-7 lines originating from two different places indicated that these cell lines were inauthentic. Possibly due to cross contamination during activity in the laboratory. This makes the two cell lines unusable as materials in in vitro studies. There are limitations to this study because it only analyzed 1 batch of subcultures for each cell line. Further research is needed in the entire batch of culture collection in the Central Laboratory for Stem Cell Studies at the YARSI University Research Institute.
Multimedia and E-Learning Innovations in Developing Tolerance in a Pandemic Era: Lessons from Pangandaran Mulyana, Asep; Mundayat, Aris Arif; Sukmajati, Mada
Nusantara Science and Technology Proceedings The 3rd International Conference on Vocational Innovation and Applied Sciences (ICVIAS) 2021
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Abstract

The spread of the Covid-19 virus has prompted the government to carry out various restrictions on social mobility, including closing schools. Recent studies have demonstrated the dynamics and problems in online learning, but very few of them reveal innovative online learning best practices. This article analyzes the innovation of multimedia learning and e-learning at SMK Bakti Karya Parigi (SBK), Pangandaran, in developing tolerance. Through phenomenology as a research strategy, the object of research is approached by giving space for experience-based subjectivity. Meanings, attitudes, beliefs, and emotions are important data components. Based on nine months of observations and in-depth interviews with stakeholders, this article discusses how multimedia learning and e-learning innovations are built during a pandemic. The results of this study indicate that this school has developed innovative programs, including the Vocational Balancing Program (VBP). Through VBP, SBK students interact in assisting Small and Medium-sized Enterprises (SMEs) in local villages to integrate with the online marketplace. They build creative content that promotes these SMEs amid the sluggishness due to the pandemic. The learning process that involves the dissemination of knowledge, discourse, experience, consciousness, and emotions is what breeds tolerance among SBK students. This study makes a theoretical contribution to the important role of knowledge and discourse, the function of consciousness and emotion, and the workings of discursive practice in the formation of educational subjects.
Efforts of Milkfish Pond Farmers in Managing Psychological Stress and Remaining Productive in The Time of the Covid-19 Pandemic Cahyono, Bagus Dwi; Huda, Nurul; Aristawati, Evy
Nusantara Science and Technology Proceedings The 3rd International Conference on Vocational Innovation and Applied Sciences (ICVIAS) 2021
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Abstract

The COVID-19 pandemic has had a far-reaching impact both directly and indirectly, including milkfish farmers. The enactment of Large-Scale Social Restrictions (PSBB) and continued with the Enforcement of Restrictions on Community Activities (PPKM) will impact them. The impact can be in the form of psychological stress that can cause a decrease in mood or work passion which has implications for a decrease in their productivity. This study aims to explore how milkfish pond farmers are trying to manage their psychological stress and remain productive during the COVID-19 pandemic. This research method is qualitative by thoroughly investigating and understanding the existing phenomena, starting from data processing, data triangulation, and data analysis. The sample of this research is milkfish pond farmers and policymakers related to milkfish cultivation. The sampling technique used is snowball sampling starting from policymakers to milkfish pond farmers until saturation or similarity of answers is found from the research subjects. The results of this study indicate that milkfish pond farmers experience psychological stress and decreased mood on levels that vary from mild to moderate. However, they are still trying to stay productive during the COVID-19 pandemic. This research implies that the stress level and mood of milkfish farmers and their coping mechanisms to decrease stress levels. The contribution of this research is as basic data for further researchers to develop applied research such as safe interventions to reduce stress levels and improve the mood of milkfish pond farmers.
Startup and MSME’s Development: Identification Based on Business Characteristics of The Millenial Generation in Padang City Andriani, Chichi; Thaib, Ilham
Nusantara Science and Technology Proceedings The 3rd International Conference on Vocational Innovation and Applied Sciences (ICVIAS) 2021
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Abstract

This study aims to identify and explain the characteristics of the start-up business of the millennial generation in the city of Padang as a process for startup and MSMEs development. The population in this study is a pioneering business for the millennial generation, namely all coffee shops in Padang city. The sampling technique used purposive sampling technique with the criteria; the coffee shop owners is a millennial generation with birth years 1995-2010, the age of the company ranges from 1-5 years, has more than 2 employees and has a profit above IDR.10,000,000/year. The number of samples in this study using the opinion of Hairs et all so that the samples used were 180 coffee shops. Research data were collected through questionnaires and data analysis using descriptive statistical analysis techniques. From the research results obtained characteristics of; gender, age, educational background, marital status, length of business establishment, number of employees, a form of business ownership, availability of wifi/internet facilities for visitors, membership status on online shopping applications, total sales per year, business ownership status, business legal entity status, separation of company finances from personal finances, business development plans, profit adequacy business and allowance for operating results. Of the 16 characteristics, it is hoped that they will be able to assist in the start-up process and MSMEs development for the millennial generation in Padang city.
Low Back Pain Therapy with Back Massage, Acupressure and Ginger Compress Aura, Jihan; Purwitasari, Neny; Adianti, Myrna
Nusantara Science and Technology Proceedings The 3rd International Conference on Vocational Innovation and Applied Sciences (ICVIAS) 2021
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Low back pain (LBP) is defined as a sensation of pain and discomfort localized below the 12th rib and above inferior gluteal folds. In this case study, based on traditional medicine the client's LBP is due to kidney deficiency syndrome especially the kidney Qi and the damp-cold pathogen attack. Which is caused by bad habits, trauma, the living environment tends to be humid that can injure the kidneys, bladder which makes the kidney Qi weak and cause damp-cold pathogens to settle easily. The purpose is to know the effect of combination therapy for a back massage, acupressure at Shenshu (BL23), Weizhong (BL 40), Taixi (KI 3), and Sanyinjiao (SP 6) and ginger (Zingiber officinale Rosc.) compress on low back pain sufferers. For the methods, the clients with LBP were given massage therapy in the back and acupressure points at Shenshu (BL 23), Weizhong (BL 40), Taixi (KI 3), and Sanyinjiao (SP 6) as well as given ginger herbal compresses (Zingiber officinale Rosc.) 12 times within 1 week 2 times of therapy. The ginger used is red ginger as much as 20 grams each time therapy. The results of the treatment of LBP were that massage combination therapy on the back area and acupressure at the points Shenshu (BL 23), Weizhong (BL 40), Taixi (KI 3), and Sanyinjiao (SP 6) and presented a ginger herbal compress (Zingiber officinale Rosc.) can help with low back pain. Conclusion: Back Massage therapy, acupressure, and ginger compress could reduce low back pain.
Dengue Hemorrhagic Fever (DHF) Risk Assessment for Gorontalo Regency in Indonesia Using Geographic Information System Pakaya, Ririn; Hano, Yanti Hz.; Olii, Muhammad Ramdhan
Nusantara Science and Technology Proceedings The 3rd International Conference on Vocational Innovation and Applied Sciences (ICVIAS) 2021
Publisher : Future Science

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Abstract

Inadequate planning, housing, water, sewage, and waste management in urban and semi-urban areas produced ideal conditions for dengue viruses and their mosquito vector Aedes Aegypti to spread throughout the region over the preceding decade. Dengue prevention is primarily focused on vector control (chemical spraying, biological control, physical removal of breeding grounds, and infrastructure enhancement) and community education in the absence of a vaccine. They have not influenced the prevalence of Dengue Hemorrhagic Fever (DHF) Public health surveillance is the systematic and continuous collection of data and information on events, health problems, and conditions that influence the occurrence and spread of disease to plan, implement, and evaluate public health practices. It is crucial in dengue management since it determines the number and distribution of cases, viral serotypes, and severity of disease in a community. The purpose of this article is to examine the use of Geographic Information Systems (GIS) to determine the spatial distribution of the DHF Risk map in the Gorontalo Regency. The GIS system's overlay operation is utilized to combine two maps, namely the DHF hazard map and the DHF vulnerability map. The findings reveal that the Gorontalo Regency is dominated by low-risk classes, with a total area of 99,716.7 ha (46.52%). The GIS approach might be used to assess transmissible DHF risk zoning, which would aid in enhancing DHF and other vector-borne disease surveillance strategies to promote prevention and control efforts.
Sipp Application Development Effort in Supporting the Digitalization of Tri Dharma at The Universitas Negeri Semarang Setiawan, Avi Budi; Widyastuti, Ariyani; Putra, Anggyi Trisnawan; uminar; Wicaksana, Tania
Nusantara Science and Technology Proceedings The 3rd International Conference on Vocational Innovation and Applied Sciences (ICVIAS) 2021
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Abstract

Technology has helped humans in various fields, for example in the field of research and service. The Institute for Research and Community Service (LPPM) Universitas Negeri Semarang (UNNES) in its role in realizing the responsibility for the Tri Dharma of Higher Education, has changed the procedure for evaluating the instrument which was originally manual to be more effective and efficient through an application called SIPP (Management Information System). Research and Service) which can be accessed on the website. This change is expected to create even better conditions. However, these existing facilities are not well received. This can be seen from the many researchers who are late in collecting research instruments and even less familiar with this SIPP application. Data analysis techniques used in this study include transcripts of the results of field surveys, observations and interviews, data reduction, analysis, data interpretation and triangulation.
Best Practice Layer 2 Security for remote Vocational Education Priambodo, Dimas Febriyan; Syamsiah, Nurul; Setiawan, Hermawan; Yulandi
Nusantara Science and Technology Proceedings The 3rd International Conference on Vocational Innovation and Applied Sciences (ICVIAS) 2021
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Abstract

Open standards especially OSI are built to separate each layer and work without knowledge of each other. This concept also means if one layer is hacked, communication is compromised without another layer alert. In best practice, layer 2 security is underrated as it is considered that security has been built through the application layer. In vocational education who are prepared to work directly in the real world, should not be forgotten to be taught. This method proposed online education for teaching layer 2 security with HyperV virtual machine to build attack and defend scenarios. Hyper V is used because windows have been installed in almost all students. In Defcon was also used HyperV was found to be able to operate to respond to attack scenarios in the second layer of security. This system was implemented in 20 students (all population in class) of security engineering in the polytechnic XYZ and shows a significant increase with normal data in statistical approach with t-test. This approach causes an increase in student scores by 12.307% by implementing a new system.
The Effect of Balanced Nutrition Feeding Time on Improving the Nutritional Status of Stunting Children Sari, Ria Setia; Ratnasari, Febi; Susilowati, Yuni
Nusantara Science and Technology Proceedings The 3rd International Conference on Vocational Innovation and Applied Sciences (ICVIAS) 2021
Publisher : Future Science

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Abstract

Stunting is a nutritional problem that has a negative impact on achieving optimal growth and development in children. One of the causes of stunting is the inaccuracy of giving balanced nutritional food. The purpose of this study was to analyze the timing of giving balanced nutritional foods to the nutritional status of stunting children, using a quasi-experimental method, one group pre, and post-test design. The sample of this research was taken by accidental sampling technique as many as 45 respondents. The analysis test used Wilcoxon with the result of Sig 0.000 (< 0.05), where there was an effect of the time of giving balanced nutrition to the nutritional status of stunting children in the work area of ??the Tangerang District Health Office.
Batak Toba Script, Preserving Its Authenticity in Globalization Stream Lubis, Donal Fernado; Bowo, Tri Arie
Nusantara Science and Technology Proceedings Multi-Conference Proceeding Series A
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This paper exposes the existence of Batak Toba's ancient script and the shift of its authenticity values during the rapid development of technology. The analysis adapts cultural and historical approaches to be exposed qualitatively by observing several transcripts virtually. Technology is viewed from two perspectives: a media that brings this ancient heritage back to be part of modern culture; and the means that lead this ancient writing to lose its authenticity. Several former documents from field and library research are compared, especially the use of several computer software, which has been developed to enable users to write in Batak Toba script through transliteration. The result reveals that efforts to conserve this cultural heritage should be accompanied by careful linguistic assistance so that the script and its writing convention would be maintained.

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