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Current Research on Biosciences and Biotechnology
Published by Institut Teknologi Bandung
ISSN : -     EISSN : 26861623     DOI : https://doi.org/10.5614/crbb
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Materials Science & Nanotechnology Medicine & Pharmacology
The Current Research on Biosciences and Biotechnology (CRBB) encourages researchers to publish works related to the use of the biosciences and biotechnology in understanding the world around us. From Health and Medicine to Advanced Materials, these state-of-the-art research offer novel insights in a multidisciplinary environment. We encourage scientists to submit papers focusing on the following broad topics: Health and Medicine Pharmaceutical Sciences Pharmacology and Toxicology Food Microbiology and Biotechnology Biotechnology Agriculture Bio-Energy Marine Sciences Advanced Materials Environmental Sciences
Arjuna Subject : Umum - Umum
Articles 77 Documents
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Isolation and characterization of lactic acid bacteria from Etawa crossbreed goat’s milk Khairan; Cut Yulvizar; Suri Raihan Safriani
Current Research on Bioscences and Biotechnology Vol. 1 No. 1 (2019)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2019.1.1/RIYP627

Abstract

Goat’s milk is white liquid derived from ruminant types of dairy goats. Milk is one of habitats of lactic acid bacteria (LAB). LAB have a potential as antimicrobial because capable to kill the pathogenic bacteria. LAB isolated from Etawa crossbreed goat’s milk were characterized to stipulate the genus of the isolates. Characterization of LAB consists of colony, morphology and biochemical assay. The morphological examination of the colony, cell morphology and biochemical assay showed that three isolates were identified as Leuconostoc, Enterococcus and Lactobacillus. The antimicrobial activity assay showed that those isolates exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli, but those isolates did not exhibit antifungal activity against Candida albicans.
Selection of bacteria inducing calcium carbonate precipitation for self-healing concrete application Ridwan Syarif; Intan Nurul Rizki; Ridho Kresna Wattimena; Siti Khodijah Chaerun
Current Research on Bioscences and Biotechnology Vol. 1 No. 1 (2019)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2019.1.1/ZDYD8792

Abstract

A modification of bacterial medium using calcium lactate pentahydrate was developed for calcium carbonate precipitation. A total of five strains of bacteria were cultivated on the solution medium containing nutrient broth and calcium lactate pentahydrate. In this study, the variation of 3.2 mM and 16.2 mM of calcium lactate pentahydrate was used to obtain the optimum condition for bacterial growth. The results showed that isolated strains CPB 1, CPB 3, and CPB 5 with medium containing nutrient broth and 3.2 mM calcium lactate pentahydrate gave the optimum growth, pH and Eh, thus being favourable for the process of calcium carbonate precipitation. Hence, this will be useful for self-healing concrete.
Luteolin, a flavonoid from Syzygium myrtifolium Walp. Raden Herni Kusriani; Shinta Maulida Rosandhy; Elfahmi
Current Research on Bioscences and Biotechnology Vol. 1 No. 1 (2019)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2019.1.1/FKAN4064

Abstract

A flavonoid, luteolin, was isolated from the leaves of Syzygium myrtifolium . The compound was purified using vacuum liquid chromatography with the help of a specific guidance for the flavonoid isolation. The chemical structure of this compound was determined based on measurements of UV, IR and NMR spectroscopic data. The occurrence of luteolin in S. myrtifolium is firstly reported from this study.
DNA isolation of potentially probiotic lactic acid bacteria from durian arilus fermentation (jruek drien) Yulia Sari Ismail; Cut Yulvizar; Risa Riani Ramlan; Mahyuddin
Current Research on Bioscences and Biotechnology Vol. 1 No. 1 (2019)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2019.1.1/UEJB8271

Abstract

Jruek drien is an Acehnese traditional fermented food containing lactic acid bacteria (LAB) from durian arilus fermentation. Lactic acid bacteria from jruek drien were considered as potential probiotics. The LAB from jruek drien were first identified by phenotyping and genotyping analysis. The aim of the research is to isolate DNA from lactic acid bacteria (JD-2 and JD-3) from jruek drien for identification based on 16S rDNA gene sequence analysis. The research steps consist of samples regeneration in MRSA medium with temperature of incubation at 37°C, samples culturing in TSB medium, DNA isolation, and measurement of the DNA concentration and DNA purity using nanophotometer. The result showed that the JD-2 and the JD-3 isolates grow well on selective media MRSA by incubation temperature at 37°C. JD-2 has a DNA concentration of 40.0 ng/μl with a purity level of 2.0, and JD-3 has a DNA concentration of 32.5 ng/μl with a purity level of 1.8 at the A260/A280 ratio. The 16S rRNA gene of JD-2 isolate was successfully amplified at 1426 bp and JD-3 isolate at 1435 bp. The JD-2 isolate was identified as Bacillus subtilis because it has the highest similarity with Bacillus subtilis strain WA3-4. The JD-3 isolate was identified as Lactobacillus plantarum because it has the highest similarity with L. plantarum strain CSI9 and strain CSI3.
Assessment of antioxidant and xanthine oxidase inhibitory activity of Triadica cochinchinensis stem bark Carla Wulandari Sabandar; Juriyati Jalil; Norizan Ahmat; Nor-Ashila Aladdin
Current Research on Bioscences and Biotechnology Vol. 1 No. 1 (2019)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2019.1.1/HZRA413

Abstract

Triadica cochinchinensis has been used traditionally to treat diseases related to oxidative stress. Nonetheless, little is known about its biological activity. This study aimed to evaluate the antioxidant and xanthine oxidase inhibitory activity of this plant. For this purpose, qualitative phytochemicals, total phenolic, total flavonoid, antioxidant assays (DPPH and FRAP), and xanthine oxidase assay were evaluated towards methanol and organic fractions of its stem bark. Results showed the occurrence of flavonoids, tannins, terpenoids, steroids, and saponins in the methanol extract. The extract and its two fractions (ethyl acetate and methanol) exhibited promising radical scavenging (IC50 values between 3.6-4.5 μg/mL) and ferric reduction activities (4.2-5.5 μg/μg of equivalent trolox amount). They also exhibited inhibition on the xanthine oxidase activity ranging from 43.8 to 80.5% at a dose of 100 μg/mL. These activities could be attributed to the amount of phenolics in the methanol extract and active fractions (136.6-174.1 mg GAE/g of sample). Our results suggested that the methanol extract of T. cochinchinensis stem bark could be used as a promising source of lead molecules for antioxidant and xanthine oxidase inhibitors from natural source.
The effect of ascorbic acid and soybean protein hydrolysate to iron binding capacity Sri Peni Wijayanti; Erma Maryana; Ida Susanti; Noer Laily
Current Research on Bioscences and Biotechnology Vol. 2 No. 1 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2020.2.1/QSEB7056

Abstract

The study aimed to investigate the effect of ascorbic acid and soybean protein hydrolysate to iron-binding capacity. BPPT (Agency for the Assessment and Application of Technology) developed soybean hydrolysate, which was obtained by thermal and enzymatic hydrolysis. Ascorbic acid was added in sample preparation with composition 0, 50, 75 and 100%. Soybean protein hydrolysate (SPH) was prepared by gel filtration chromatography using Sephadex G-25 to separate peptide based on molecular weight. Characterization of SPH and SPH fractions was also conducted, such as protein concentration, molecular weight, iron-binding capacity, and amino acid concentration. Iron binding capacity was determined by the colorimetric method using an ortho-phenanthroline reagent. The result showed that soybean protein hydrolysate increases iron-binding capacity 25-37 times than ascorbic acid. Arginine, aspartic acid, glutamic acid, threonine, lysine, and serine were found as amino acids responsible for iron-binding capacity in the SPH.
Phenazine-1-carboxylic acid (PCA) from cold-adapted yeast Glaciozyma antarctica PI12 Andi Rifki Rosandy; Rozida Mohd. Khalid
Current Research on Bioscences and Biotechnology Vol. 2 No. 1 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2020.2.1/TLCY1641

Abstract

A phenazine alkaloid, phenazine-1-carboxylic acid (PCA) was isolated from the ethyl acetate extract of cold-adapted yeast Glaciozyma antarctica PI12 (Kriegeriales). The compounds were isolated by several chromatography techniques i.e. radial chromatography (RC) and thin-layer chromatography (TLC). The chemical structure was elucidated by ultraviolet (UV), infrared (IR), nuclear magnetic resonance (1D and 2D NMR) spectroscopy and mass spectrometry.
Increasing the capacity of natural killer (NK) cells in fighting advance stage ovarian cancer: A cellular immunotherapy minireview Radiana Dhewayani Antarianto; Fransisca Dela Verna; Lady Feren Pangjaya; Sanya Khaerunnisa; Dinda Shezaria; Tricia Dewi Anggraeni
Current Research on Bioscences and Biotechnology Vol. 2 No. 1 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2020.2.1/SPNZ3413

Abstract

Global Cancer Statistics in 2018 estimated 300,000 new cases of ovarian cancer with 152,000 mortality rate each year. The Indonesian Society of Gynecologic Oncology reported 30% of gynecologic cancer is ovarian cancer, which has a 125, 000 mortality rate each year. Ovarian cancer data in Indonesia showed that 70% of patients were diagnosed with ascites or metastasis beyond the ovaries (stage III or IV). Ovarian cancer is an immunogenic disease with an immunotherapy intervention on the horizon. To assess the potency of stimulated NK cells as ovarian cancer cellular immunotherapy, literature search was collected from NCBI, ScienceDirect and Pubchem database. A total of 19 articles relevant to our search terms were included in this review. NK cells from ovarian cancer ascites exhibit low cytotoxic efficacy but can be restimulated using IL-2 or IL-15. An in vitro study that incubated NK cells with an IL-15 fusion protein enhanced the function of the ovarian cancer ascites’ NK cells or the healthy NK cells against the ovarian cancer ascites cells. Human IL-12-, IL-15- and IL-18-induced memory like (CIML) NK cells, has been proven to increase the elimination of xenograft human ovarian cancer cells over a long period of time in a mouse model. CIML NK cells also showed higher NK cell expansion and an enhanced function in the ovarian cancer ascites’ microenvironment, which was immunosuppressive. Phase I-II clinical trials on NK cell-based adoptive cellular therapies demonstrated limited clinical benefit. The major challenges are obtaining persistent NK cells with anticancer activity.
Phytochemistry and pharmacological activities of Annona genus: A review Siti Kusmardiyani; Yohanes Andika Suharli; Muhamad Insanu; Irda Fidrianny
Current Research on Bioscences and Biotechnology Vol. 2 No. 1 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2020.2.1/KNIA7708

Abstract

Plants have been significantly used in traditional medicine by a variety of societies since antiquity, and knowledge of their safety, efficacy, and quality value can be developed through further research. The Genus Annona consisting of 119 species has been extensively researched and proven to have a diverse range of pharmacological activities such as antioxidant, antiulcer, antidiarrheal, and antiparasitic. This is because the Annona plants possess a great number of phytochemicals found in almost every part of the plant, which can be isolated to be developed into herbal medicine. Phytochemicals are classified into several classes, such as Annonaceous acetogenin, alkaloids, flavonoids, and essential oils. This article was created by collecting 137 research articles which discuss phytochemical compounds from 38 species and the pharmacological activity from 13 species.
The effect of temperature, incubation and storage time on lactic acid content, pH and viscosity of goat milk kefir Yola Desnera Putri; Nur Asni Setiani; Sohadi Warya
Current Research on Bioscences and Biotechnology Vol. 2 No. 1 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2020.2.1/HPMQ5042

Abstract

Kefir is a fermented beverage that has probiotic properties and often used as a cosmetic or ointment raw material. Its lactic acid content is classified as AHA (alpha hydroxy acid) which is known good for skin health. The objectives were to study the influence of temperature, fermentation and storage time on the amount of lactic acid, pH and viscosity of kefir. Fermentation of kefir was performed at two different temperatures (room temperature and 37oC) for 24 and 48 h. Storage condition of kefir products was performed at cold and room temperature for 4 to 28 d. The content of lactic acid was based on the total organic acid determined using acid-base titration. The results showed that the average content of lactic acid in 48 h-fermented kefir at room temperature and 37oC were 0.9 to 2.2% with pH and viscosity characteristics were 4.1 to 4.3 and 1400 to1600 cPs, respectively. Meanwhile, during 24 d of storage, the average content of lactic acid was 1.97 to 3.54%, where pH and viscosity characteristics were 3.5 to 4.5 and 3400 to 6400 cPs, respectively. The optimum storage time of goat milk kefir is obtained on days 4 to 12 and they can be stored for up to 24 days without deterioration of kefir products.

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