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Current Research on Biosciences and Biotechnology
Published by Institut Teknologi Bandung
ISSN : -     EISSN : 26861623     DOI : https://doi.org/10.5614/crbb
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Materials Science & Nanotechnology Medicine & Pharmacology
The Current Research on Biosciences and Biotechnology (CRBB) encourages researchers to publish works related to the use of the biosciences and biotechnology in understanding the world around us. From Health and Medicine to Advanced Materials, these state-of-the-art research offer novel insights in a multidisciplinary environment. We encourage scientists to submit papers focusing on the following broad topics: Health and Medicine Pharmaceutical Sciences Pharmacology and Toxicology Food Microbiology and Biotechnology Biotechnology Agriculture Bio-Energy Marine Sciences Advanced Materials Environmental Sciences
Arjuna Subject : Umum - Umum
Articles 77 Documents
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Isolation, identification and biochemical studies of gallic acid from Turbinaria decurrens Bory Fitriyanti Jumaetri Sami; Syamsu Nur
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/X7DD09RY

Abstract

Cancer is one of the diseases that cause the biggest death in the world. The research for natural compounds derived from marine is very developed at this time, especially in algae. Brown algae have more phenolic content than other types of algae, T. decurrens Bory is one of the brown algae that had taken from the Indonesian island of Dutungan, South Sulawesi. Phenolic groups are also known to be able to inhibit the growth of cancer cells. The purpose of this study is to isolate and identify compounds using spectrophotometer instruments. The isolates obtained were tested for anticancer activity against H460 and MCF-7 cells. Extraction was carried out by maceration method, fractionation was done by chromatography column eluent n-hexane:EtOAc: MeOH by increasing polarity. The structure determination is done based on the interpretation of FT-IR, 1D-NMR 1H,13C, and ESI-LCMS spectra data and anticancer activity test used MTT bioassay. The isolate is known as gallic acid, and the isolate was tested for anticancer activity against H460 and MCF-7 cells. The result is obtained IC50 value for H460 cells 5.69 μg/ml and MCF-7 cells 4.63 μg/ml. As a positive control used cisplatin with an IC50 value of 5.81 μg/ml against H460 cells and MCF-7 cells with a value of 5.59 μg/ml. Gallic acid has a higher toxic effect compared to cisplatin. One phenolic compound has been found in T. decurrens Bory which is gallic acid. Gallic acid has a higher toxic effect than cisplatin, this compound can be used as an anticancer agent.
Lipid content and fatty acid profile of commonly consumed freshwater and marine fish of Bangladesh Mohammad Shoeb; Md. Mazharul Islam; Khandoker Tahmina Tasnim; Sunjida Akter; Md. Iqbal Rouf Mamun
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/J1LQ0XTP

Abstract

The objectives of this research were to determine total lipid content and corresponding fatty acid profiles in freshwater (n=10) and marine fish (n=11) samples commonly consumed in Bangladesh. Lipid was extracted by Solid Phase Dispersion method. Saponification and esterification were carried out by the association of official analytical chemists (AOAC) reference procedure with some simple modifications. The fatty acids were analyzed as their methyl ester by gas chromatography equipped with flame ionization detector (GC-FID) by comparing the retention time of 13 standard methyl ester of fatty acids. Total lipid content was 0.97-8.05% and 0.97-4.33% in freshwater and marine fish, respectively. Unsaturated fatty acid in freshwater fish was found in highest amount than saturated fatty acid. Among unsaturated fatty acids, palmitoleic acid was found in highest concentration and ranged from 46.80-26.02%. Palmitic acid was found in highest amount among saturated fatty acids and ranged from 3.96-13.91%. Among the marine fishes unsaturated fatty acids (MUFAs and PUFAs) i.e; palmitoleic acid (26.21-41.61%), oleic acid (2.35-29.63%) and linoleic acid (1.40-14.45%) were predominant. Among the saturated fatty acids myristic acid (0.97-9.08%), palmitic acid (1.61-11.35%) and stearic acid (1.03-21.53%) were found to be predominant.
Antihypertensive activity and acute toxicity of turmeric (Curcuma longa L.) in L-NAME-induced hypertension animals Patonah Hasimun; Yani Mulyani; Irma Yulianti
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/B2AMBRI1

Abstract

Hypertension is one of the significant risk factors for cardiovascular disease and can lead to complications. Uncontrolled hypertension can lead to vascular endothelial dysfunction and other complications of cardiovascular disease. This study aimed to determine the effect of turmeric rhizome (Curcuma longa L.) in extracts and the fractions on a hypertensive rat’s model induced by L-NAME 40 mg/kg for three weeks and an acute toxicity study of the extract. Antihypertensive research was performed on male Wistar rats utilizing non-invasive procedures. Turmeric extract at doses of 50, 100, and 200 mg/kg and its fraction of n-hexane, acetyl acetate, and ethanol at a dose of 25 mg/kg, respectively, were given daily per oral for three weeks to 2.5 mg/kg captopril. The systolic and diastolic blood pressure were measured at 0, 21, and 42 days after treatment and was calculated as mean arterial blood pressure (MAP). Acute toxicity testing refers to the OECD 420 Fixed-Dose method with several dosage levels, consists of 300, 2000, and 5000 mg/kg. The turmeric extract at doses of 50, 100, and 200 mg/kg and its fraction of n-hexane, acetyl acetate, and ethanol significantly reduced mean arterial blood pressure (p<0.05) compared to the control group. Acute administration of turmeric extracts up to a dose of 5000 mg/kg in test animals did not show any death. Turmeric and its components are considered to possess antihypertensive actions. Antihypertensive activity increased in a dose-dependent manner. Turmeric extract is categorized as being almost entirely non-toxic.
Biological activity studies of the aerial parts of Phyllanthus niruri L. Md. Nazrul Islam; Mohammad Shoeb; Nilufar Nahar
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/U7GMG56E

Abstract

Phyllanthus niruri L. is a small annual plant grows up to 30-40 cm tall. It is an herbaceous plant that is widely distributed in the tropical and sub-tropical regions. The aerial parts of P. niruri growing in Bangladesh were investigated for moisture content and ash content, total phenolic content, total flavonoid content, total antioxidant capacity (TAC), cytotoxicity, relative fatty acid compositions and antibacterial activity. The moisture and ash content of the dried leaves were 5 and 7%, respectively. The extracts of P. niruri contained a good amount of phenolic and flavonoid content and revealed significant antioxidant capacity. The highest TAC was shown by dichloromethane fraction (131.3±9.20) and it was followed by 80% ethanol extract (126.1±6.25) and then ethyl acetate fraction (122.5±10.65). The highest amount of phenolic content was present in ethyl acetate fraction, whereas the lowest amount was in hexane fraction. Cytotoxicity was found in 80% ethanol extract, dichloromethane and aqueous fractions. Fatty acids like lauric acid (0.5%), tetradecanoic acid (1%) palmitoleic acid (37%),octadecanoic acid (57%) cis-9-oleic acid (5%) were identified and quantified in hexane fraction of P. niruri by GC-FID and octadecanoic acid was predominant among all acids. The antibacterial activity of 80% ethanol extract and aqueous fraction of P. niruri was tested against two Gram negative (Escherichia coli, Salmonella typhi) and two Gram positive (Bacillus subtilis and Staphylococcus aureus) bacteria following agar diffusion method. The results revealed weak antibacterial activity.
Method development of quercitrin enrichment from asthma-plant (Euphorbia hirta L.) using aromatic macroporous resin Sumail Sidik Ode Ishak; Amrianto; Diah Astari Salam; Rika Hartati
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/2G0E50F6

Abstract

Asthma-plant contains high amount of quercitrin which make it a potential new source for flavonoids. This study aims to develop a method of quercitrin enrichment by utilizing macroporous resin, which is known to be safer, more eco-friendly, economics, and efficient. Evaluations were conducted over the performance and separation characteristics of the macroporous resin in quercitrin enrichment as well as the adsorption and desorption of quercitrin by the macroporous resin. The results showed that the adsorption process of the macroporous resin in relation to the amount of quercitrin in the extract were in accordance with the second order model, which means that the process of adsorption is affected by other compounds. Furthermore, the examination of the isotherm adsorption fit the Freundlich’s model (R2 = 0.9850) rather than the Langmuir’s one (R2 = 0.4334). In the optimal condition, the enrichment of quercitrin by using macroporous resin increased the abundance of quercitrin by nearly five times, from 3.60% of quercitrin content in the extract to 17.02% in the quercitrin-rich fraction, with recovery yield of 50.39%.
Effective removal of chromium from contaminated water using horizontal subsurface flow constructed wetland with Napier grass (Pennisetum purpureum) Taufik Taufikurahman; Lili Melani; Natasha Lumongga Hugo Sianturi; Ni Komang Devi Amrita Wirawan; Aisyah Fairuz Saifullah
Current Research on Bioscences and Biotechnology Vol. 4 No. 2 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.4.2/226W5GYH

Abstract

This study aims to explore the potential of using Napier grass (Pennisetum purpureum) as a low-cost alternative for treating wastewater contaminated with chromium (Cr) in the tannery industry. Traditional chemical treatment processes are costly, leading to many industries not committing to treatment. Phytoremediation, the use of plants to remove contaminants from the environment, could be a viable solution. The study conducted an experiment to test the tolerance and effectiveness of P. purpureum in treating wastewater containing Cr(VI) at concentrations of 20 ppm, 40 ppm, and tannery wastewater. The plant was grown in a constructed wetland pond using a batch system for 31 days. The results indicate that P. purpureum can survive and grow well in all treatments, showing tolerance to Cr. The plant's phytoremediation capacity was effective at 20 ppm of Cr(VI) as demonstrated by the chemical and physical characteristics of the medium (BOD, COD, pH, and TDS). The plant was able to remove a significant amount of total Cr and Cr(VI), with removal capacity ranging from 71.79% to 99.96%. The translocation factor (TF) for Cr in the roots was less than one, indicating that the plant has the potential to be used as a phytoremediator for Cr by accumulating it in its roots.
Effects of pre-treatment with Aspergillus awamori and extraction methods on essential oil yield from spearmint leaves (Mentha spicata L.) Yasriza Nanda; Rian Fiqraini; Kelvin Alfianza; Rijanti Rahaju Maulani; Noor Illi Mohamad Puad; Muhammad Yusuf Abduh
Current Research on Bioscences and Biotechnology Vol. 4 No. 2 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.4.2/DDQZKJJY

Abstract

Extraction of essential oil from spearmint leaves is typically hindered by the presence of cell wall composed of lignocellulose which can be biologically degraded by microorganisms. This study aimed to investigate the effects of fermentation using Aspergillus awamori towards the lignocellulosic content of spearmint (Mentha spicata L.) leaf as well as spearmint oil yield and composition, and diffusion coefficient obtained using different extraction methods. Fermentation of the spearmint leaves were carried out for 3, 6, and 9 days followed by drying and extraction using three different techniques particularly Soxhlet, hydrodistillation and maceration. After fermentation, the cellulose, hemicellulose, and lignin reduced from 37.92% to 19.32%, 13.98% to 5% and 27.20% to 12.24%, respectively. The yield of spearmint oil varies from 0.35% to 2.10% for maceration, 0.22% to 1.83% for Soxhlet and 0.07% to 0.58% for hydrodistillation with a maximum yield (2.10%) was obtained using a maceration method after 9 days of fermentation. The composition of spearmint oil has been determined and contains carvone as the major compound up to 77.88%. In addition, the diffusion coefficients for extraction of spearmint oil using the different extraction methods have been estimated and lies in the range of 2.89 x 10-11 m2/s to 3.64 x 10-11 m2/s. Hence, the fermentation of spearmint leaves using A. awamori decreased the lignocellulose content and thereby increased the yield of spearmint oil. In addition, the composition spearmint oil and diffusion coefficients of the extraction process have been determined.
Development of analysis method of andrographolide from Andrographis paniculata using UPLC-PDA Syefira Salsabila; Diah Astari Salam; Sumail Sidik Ode Ishak; Amrianto
Current Research on Bioscences and Biotechnology Vol. 4 No. 2 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.4.2/I9YWKM9X

Abstract

Andrographis paniculata is an herbaceous plant belonging to the family Acanthaceae dan also known as the “King of Bitters”. It has been utilized as traditional medicines by the local people in India and Southeast Asia. Many studies have been reported regarding the pharmacological activities of A. paniculata and Andrographolide. Currently, in addition to compound isolation and bioactivity assays, validation of analysis method is also required. Validation is used to demonstrate that the method used is appropriate and the results are precise and accurate. The method used needs to be simple, concise, precise, accurate, and widely used. The analysis in this study was carried out using Ultra-Performance Liquid Chromatography because the instrument operates at high pressure. In addition, the system uses fine particles which can reduce column length as well as solvent and time consumption. This study aims to validate analytical methods in determining the contents of andrographolide in an A. paniculata Leaves. The analytical method was validated through a Waters Alliance UPLC System with photodiode array (PDA) detector. The A. paniculata extract and standard solutions of Andrographolide were analyzed using reverse-phase C18 column which was maintained at 40°C. A mobile phase of 0.1% formic acid in acetnotirile and 0.1% formic acid in water was used at flow rate of 0.3 mL/min to achieve gradient elution. The linearity of the andrographolide showed excellent results (R2= 0.9999) in the concentration range of 7.8–250.0 µg/mL. The LOD and LOQ values of andrographolide were 0.068 µg/mL and 0.205 µg/mL, respectively. The intra-day and inter-day relative standard deviation (RSD) and relative error (RE) accuracy values of andrographolide were <±15. The quantitative analysis found that A. paniculata extract contained 12.45 ±0.06 mg/g of Andrographolide. This developed UPLC-PDA method was proven to be precise, specific, sensitive, and accurate for routine quality assessment of raw material of Andrographis paniculata leaves content.
Effect of PGPM and goat manure doses on biomass yield and rosmarinic acid content in cat's whiskers plant (Orthosiphon aristatus Bl. Miq) Ima Mulyama Zainuddin; Millennio Salsabil; Mohammad Ghifary Muktabar; Dadang Sumardi
Current Research on Bioscences and Biotechnology Vol. 4 No. 2 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.4.2/LIGQIUMZ

Abstract

Cat’s whiskers plant (Orthosiphon aristatus B. Miq) is a medicinal plant containing various metabolites that act as antioxidants and anti-inflammatory, including rosmarinic acid. The domestic market demand for dry leaves was 20-40 tons and abroad it was 141-180 tons in the 1990-2005 period, while production from 2013 to 2017 decreased from 116 tons to 56 tons. Therefore, it is necessary to increase the cat's whiskers biomass production, one of which can be done through the engineering of growing media using organic fertilizers, including goat manure and microbes, e.g., Plant Growth Promoting Microorganisms (PGPM). This study aims to determine the optimal combination of PGPM – Goat Manure for biomass and the rosmarinic acid content in O. aristatus. The study was conducted using a 2-factor randomized block design (RBD) with 12 treatment combinations and 3 repetitions on cultivated land in the Tanjungsari area and analysis of the results was carried out in the SITH laboratory from October 2020 to March 2021. The rosmarinic acid content in the leaves ranged from 144.43 – 206.31 mg/g or 14.44 – 20.63%, with the highest content found in leaves treated with 3 ml/L PGPM and 86.2 g/plant goat manure fertilizer (P2O2).
Development and validation of analysis method for sennoside B in Cassia angustifolia using UPLC-MRM/MS Amrianto; Kurnia Nastira Ningsih; Sumail Sidik Ode Ishak; Ula Aulia Fitrian; Syefira Salsabila; Diah Astari Salam
Current Research on Bioscences and Biotechnology Vol. 4 No. 2 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.4.2/22AMMLH7

Abstract

Cassia angustifolia is a commonly found wild plant from the family Caesalpinaceae that originates from Yemen and Hadramaut province in Southern Arabia, where it is called Arabian senna. The leaves of the plant have been used to treat a variety of ailments such as constipation, malaria, anemia, loss of appetite, and indigestion. Sennosides A and B are the major glycosides found in the leaves and pods of C. angustifolia and are important ingredients in purgative medicines. These compounds are considered as the major active components of Cassia plants and are responsible for their therapeutic activities. To assess the quality and quantity of sennosides A and B, an appropriate analytical method is required, which must be simple, accurate, precise, and widely used. The UPLC-ESI-MRM/MS method was used in this study to validate the analytical method in determining the contents of Sennoside B in senna leaves extract. The validation parameters included specificity, system suitability, linearity, sensitivity (LOD, LOQ), accuracy, and precision. The results indicated that the optimization of MRM using the direct infusion method provided good separation when eluted using liquid chromatography. The validation parameters for system suitability obtained RSD under 2%. The linearity of sennoside B showed excellent results (R2 = 0.999) in the concentration range of 0.98–62.5 µg/ml. The LOD and LOQ values of sennoside B were 0.011 µg/mL and 0.034 µg/ml, respectively. The accuracy values of sennoside B met the predetermined criteria, with RSD < 2% and % recovery of 97-102%. The quantitative analysis revealed that Cassia angustifolia extract contained 0.43 ±0.06 mg/g of sennoside B.

Page 5 of 8 | Total Record : 77

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