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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,428 Documents
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Purple Yam (Dioscorea alata) Tuber Improves the Histological Appearance of the Ampulla of the Uterine Tube in a Mouse Model of Endometriosis Makiyah, Sri Nabawiyati Nurul; Usman, Sherly; Adawiyah, Rabiatul
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.20007

Abstract

This study aimed to examine the effect of Dioscorea alata ethanol extract (EEDA) on the histological features of the ampulla of the uterine tube in a mouse model of endometriosis. This was a true experimental study with a posttest-only control group design using 30 mice divided into six groups: a normal control group (K), a negative control group (KN) induced with endometriosis, a positive control group (KP) induced with endometriosis and treated with letrozole, and three treatment groups induced with endometriosis and administered EEDA at doses of 50, 250, and 500 mg/kg body weight. The observed parameters included inflammatory cell infiltration, epithelial damage, and fibrosis. Data were analyzed using the Kolmogorov–Smirnov test for normality, which showed that the data were not normally distributed; therefore, the nonparametric Kruskal–Wallis test followed by the Mann–Whitney post hoc test was applied. The results showed that the KN group had the highest scores for inflammatory cell infiltration, epithelial damage, and fibrosis (p < 0.05). Administration of EEDA significantly reduced all three parameters compared with the KN group (p < 0.05), with histological improvement increasing in a dose-dependent manner and the most optimal dose observed at 500 mg/kg body weight. It can be concluded that Dioscorea alata ethanol extract may improve the histological features of the ampulla of the uterine tube through anti-inflammatory and antifibrotic mechanisms at an optimal dose of 500 mg/kg body weight; however, the specific molecular mechanisms still require further verification. These findings indicate the potential of Dioscorea alata ethanol extract as a candidate herbal-based alternative therapy in an experimental model of endometriosis.
The Effects of Different Aquaponic System Designs (NFT and DWC) on Water Quality, Growth, and Survival of Nile Tilapia (Oreochromis niloticus) Lubis, Kaisa Alhani; Siswoyo, Bambang Hendra; Afriani, Dwi Tika
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.20014

Abstract

This study aimed to evaluate the effects of different aquaponic system designs, namely Nutrient Film Technique (NFT) and Deep Water Culture (DWC), on water quality, growth, survival rate, and feed efficiency of Nile tilapia (Oreochromis niloticus). The experiment was conducted for 30 days in the Wet Laboratory of the Aquaculture Study Program, Faculty of Fisheries, Dharmawangsa University, using a Completely Randomized Design (CRD) with three treatments and three replicates: control (without hydroponics), DWC, and NFT. The observed parameters included absolute weight gain, absolute length gain, specific growth rate (SGR), survival rate (SR), feed conversion ratio (FCR), and water quality (temperature and pH). Data were analyzed using ANOVA at a 95% confidence level (α = 0.05). The results showed that differences in aquaponic system design had a significant effect (P < 0.05) on the growth and survival of Nile tilapia. The DWC treatment produced the highest absolute weight gain of 8.5 g with a survival rate of 100%, whereas the NFT system resulted in a weight gain of 5.8 g with a survival rate of 80%. The lowest FCR was recorded in the DWC system (1.00), compared with NFT (1.07) and the control (1.25). Water quality remained within the optimal range throughout the study, with temperatures of 26–31°C and pH values of 6.6–7.2. It can be concluded that the DWC system was more effective than the NFT system and the control in improving the growth and survival of Nile tilapia.
Effect of Supplementing Feed with Kepok Banana Peel Extract (Musa balbisiana Colla) on the Growth and Survival Rate of Nile Tilapia (Oreochromis niloticus) Fry Rahmatillah, Nur Putih; Syafitri, Emmy; Afriani, Dwi Tika
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.20015

Abstract

This study aimed to analyze the effect of kepok banana peel extract (Musa balbisiana Colla) as a feed additive on the growth and survival of Nile tilapia (Oreochromis niloticus) fry, as well as to identify the optimum dosage. The study was motivated by the high cost of feed and the underutilization of agricultural waste as a functional feed ingredient. An experimental method employing a completely randomized design (CRD) was used, consisting of four treatments and three replicates: feed without extract (control) and feed supplemented with kepok banana peel extract at doses of 150, 200, and 250 g/kg feed. The experiment involved 12 experimental units stocked with Nile tilapia fry with an initial average size of ±3 cm and reared for 28 days. The observed parameters included absolute weight gain, absolute length gain, and survival rate. Data were analyzed using analysis of variance (ANOVA), followed by the least significant difference (LSD) test at the 5% significance level. The results showed that supplementation with kepok banana peel extract had a highly significant effect (P<0.01), indicating that the treatments produced statistically significant differences at a 99% confidence level. Further analysis revealed that each treatment differed significantly, with growth increasing in line with increasing extract dosage. The treatment at a dose of 25% per kg feed produced the highest growth, with an absolute weight gain of 5.43 g, an absolute length gain of 4.43 cm, and a survival rate of 86.6%. Water quality remained within the optimal range throughout the study. These findings indicate that kepok banana peel extract has potential as a natural feed additive that supports waste utilization and the sustainability of Nile tilapia aquaculture.
Effects of Garlic (Allium sativum) Extract on the Growth and Survival of Nile Tilapia (Oreochromis niloticus) Fingerlings at High Stocking Density Anggriani, Dinda Shafira; Afriani, Dwi Tika; Syafitri, Emmy
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.20016

Abstract

This study aimed to evaluate the effect of garlic extract supplementation as an immunostimulant and feed additive on the survival of Nile tilapia fry reared in an intensive aquaculture system under high stocking density, and to determine the most effective dosage. The study employed an experimental method using a Completely Randomized Design (CRD) consisting of four treatments with three replicates: no extract, 5 g/kg, 10 g/kg, and 15 g/kg of feed administered over a 28-day rearing period. The primary parameter observed was survival rate, supported by growth data. Data were analyzed using ANOVA at a 95% confidence level, followed by the Least Significant Difference (LSD) test. The results showed that garlic extract supplementation improved survival compared with the control, with values of 53.3%, 61.7%, 70.0%, and 65.0%, respectively. The treatment with 10 g/kg feed produced the highest and most optimal survival rate. A higher dose did not result in further improvement, presumably because of reduced feed palatability and the physiological response of the fish. It can be concluded that garlic extract supplementation significantly affected the performance of Nile tilapia fry, with 10 g/kg identified as the most effective dose for enhancing resistance to environmental stress. This study also provides a practical contribution by recommending an optimal dose of natural feed additive for intensive aquaculture systems.
Development of a Gastropod Diversity Booklet from Lagundri Beach, South Nias, as a Biology Learning Resource for Senior High School on the Topic of Biodiversity Buulolo, Diana; Sinambela, Masdiana; Hasruddin
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.20021

Abstract

This study aimed to identify the diversity of gastropods at Lagundri Beach, South Nias, and to develop and evaluate the feasibility, practicality, and effectiveness of a booklet as a biology learning resource for senior high school students on the topic of biodiversity. This research employed a Research and Development (R&D) approach using the 4D model (define, design, develop, disseminate). The research subjects consisted of five validators, two teachers, and 31 students. Data were analyzed quantitatively and qualitatively through validity, practicality, and effectiveness tests, with effectiveness assessed using normality, homogeneity, t-test, and N-gain analyses. The results showed that the gastropod diversity index was 3.31, which falls into the high category. The developed booklet was classified as highly feasible (average >88%), highly practical (≥91%), and moderately effective, with an N-gain value of 63.13%. The t-test indicated a significant difference between the experimental and control classes (t = 4.80; p < 0.05). Therefore, this booklet based on local potential is suitable for use as a supplementary learning resource to improve students’ conceptual understanding and biodiversity literacy.
Phylogenetic Investigation of Megapodius cumingii from Kabetan, Central Sulawesi, Based on the RDP1 Gene Akram; Budiarsa, I Made; Messe, Yunita
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.20024

Abstract

This study investigated the phylogenetic relationships of Megapodius cumingii using bioinformatic analyses. Blood samples were collected from the Kabetan Island population, followed by DNA extraction using the QIAamp DNA Blood Mini Kit, PCR amplification with Takara Ex Taq™ and RDP1.U1/RDP1.L1 primers, and sequencing with BigDye Terminator v3.1 ABI PRISM 3100. Chromatograms were converted into nucleotide sequences using BioEdit, and sequence similarity was assessed using NCBI BLAST. Multiple sequence alignment, model selection, nucleotide composition analysis, and phylogenetic reconstruction were performed in MEGA 12. The RDP1 gene of M. cumingii from Kabetan showed a consistent nucleotide composition with high GC content (55.27%) and high similarity to M. cumingii sequence KF834037.1, with substitutions dominated by transitions (A↔G, T↔C). Maximum likelihood analysis under the T92+G model (1.03) revealed three major clades, with clades 1 and 2 strongly supported (bootstrap = 100). The Kabetan population formed a monophyletic subclade with KF834037.1 (bootstrap = 86) and showed a very short branch length (~0.05 substitutions/site), indicating low intraspecific divergence, with Megapodius tenimberensis as the closest sister taxon. These findings support the utility of RDP1 for phylogenetic resolution within Megapodiidae and provide a preliminary basis for conservation planning and population connectivity assessment of M. cumingii in Wallacea.
Orthogonal Stimulus Response Method Optimization of Essential Oil Combinations from Magnolia alba, Cryptocarya massoia, and Melaleuca alternifolia for Antioxidant and Anti-Cutibacterium acnes Activities Ayudianti, Prida; Rahmayanti, Mayu; Maulina, Novia; Muslikh, Faisal Akhmal; Aluzia, Aufary Naurah; Azzahra, Fatimah; Ma’arif, Burhan
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.20029

Abstract

This study aimed to optimize a combination of white champaca oil (Magnolia alba), massoia bark oil (Cryptocarya massoia), and tea tree oil (Melaleuca alternifolia) as antibacterial and antioxidant agents using the Orthogonal Stimulus Response Method (OSRM) with a Taguchi L4 (2³) design. Antibacterial activity was evaluated using the well diffusion method against C. acnes, while antioxidant activity was assessed using the DPPH radical scavenging assay. The results demonstrated that all variations of the CMT oil combinations exhibited moderate antibacterial activity and strong antioxidant activity, with IC₅₀ values ranging from 50 to 100 µg/mL. Based on the Taguchi analysis, the optimal combination for antibacterial activity was obtained at concentrations of 6% champaca oil, 0.4% massoia oil, and 5% tea tree oil. Meanwhile, the most optimal and stable antioxidant activity was achieved with the combination at concentrations of 75:100:100 ppm. In both assays, champaca oil was identified as the most influential factor affecting the observed biological responses. These findings indicate that the CMT oil combination has promising potential as a multifunctional therapeutic candidate for cosmetic and pharmaceutical applications, particularly as a topical nanoemulsion face mist for the management of acne while simultaneously preventing skin damage caused by free radicals.
Analysis of Formaldehyde Content in Tofu Products as an Indicator of Food Safety in Pidie Regency Zulfajriana; Sardi, Arif; Harahap, Diannita
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.20037

Abstract

This study aimed to identify the presence of formaldehyde in white tofu circulating in Pidie Regency as an indicator of food safety. The research employed a descriptive design with an exploratory approach, using qualitative testing through two techniques: the use of red dragon fruit peel filtrate as a natural indicator in the initial stage and a rapid test kit as a confirmatory method. The samples consisted of 48 white tofu samples collected from 12 sub-districts, with each sub-district represented by four samples obtained from different producers. Sampling was conducted purposively by considering market activity level, number of vendors, and ease of site access. The samples were prepared through a homogenization process prior to testing. The presence of formaldehyde was determined based on color change, reaction time, and color intensity observed during the testing process. The results showed that 12 samples (25%) were positively detected as containing formaldehyde, while the remaining 36 samples (75%) tested negative. Positive samples were generally found in areas with relatively high distribution and trading activity. Based on these findings, it can be concluded that formaldehyde is still present in a proportion of white tofu sold in Pidie Regency. Therefore, enhanced monitoring and education for business actors are needed. In addition, red dragon fruit peel filtrate shows potential as a simple, economical, and environmentally friendly preliminary screening method.

Page 143 of 143 | Total Record : 1428

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