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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,493 Documents
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The Effect of the Jigsaw Cooperative Learning Model on Students’ Learning Outcomes in Ecology and Biodiversity Putri Mei Syarah; Rivo Hasper Dimenta; Novi Fitriandika Sari
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20698

Abstract

This study aimed to analyze the effect of the Jigsaw cooperative learning model on students’ learning outcomes in Ecology and Biodiversity among seventh-grade students at MTsN 1 Labuhanbatu. A quantitative approach was employed using a quasi-experimental design with a pretest-posttest control group. The sample consisted of 64 students from two classes: Class VII-I as the experimental group and Class VII-F as the control group, selected through cluster random sampling. Data were collected using a validated and reliable multiple-choice achievement test. Data analysis involved prerequisite testing and hypothesis testing using SPSS version 25. The results showed that the mean posttest score of the experimental group was 80.62, which was higher than that of the control group, with a mean score of 59.87. The hypothesis test indicated that the Jigsaw cooperative learning model significantly improved students’ learning outcomes in Ecology and Biodiversity compared with conventional teaching methods, as indicated by Sig. (2-tailed) = 0.000 (p < 0.05). These findings suggest that students’ engagement in home groups, expert groups, and peer-teaching activities contributed to improved conceptual understanding of Ecology and Biodiversity. This study implies that the Jigsaw cooperative learning model can serve as an alternative instructional strategy to enhance students’ learning outcomes and active participation in science learning.
Microplastics in the Digestive Tract of Eels (Anguilla sp.) from Lake Poso, Central Sulawesi, and Their Use as Educational Media Felisia Talantu; Fatmah Dhafir; Masrianih; Abd. Hakim Laenggeng; Rafiqa; Amalia Buntu
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20701

Abstract

This study aimed to analyze the occurrence and abundance of microplastics in the digestive tract of eels (Anguilla sp.) from Lake Poso and to implement the findings as an educational poster-based learning medium. A quantitative descriptive method was employed using six eel samples collected from the Pamona area. Microplastic analysis was conducted through organic matter digestion using 10% KOH solution, filtration with Whatman No. 42 filter paper, and microscopic particle identification. The results showed that all samples (100%) were contaminated with microplastics, with a total of 221 particles and an average abundance of 36.8 particles per individual. The identified microplastic types consisted of fibers (56.56%), fragments (23.53%), and films (19.91%), while pellets were not detected. The dominance of fibers suggests that the main sources of contamination were likely domestic and fishing activities around Lake Poso. The research findings were subsequently developed into an educational poster, which obtained an average feasibility score of 84% and was categorized as highly feasible. These findings contribute to scientific knowledge on microplastic contamination in freshwater eels and demonstrate the potential of local research findings to serve as contextual learning resources for improving students’ environmental literacy and awareness.
Effect of the Think Talk Write Cooperative Learning Model Assisted by Poster Media on Students’ Learning Outcomes in Biology Among Grade VIII Students at SMP Negeri 19 Palu Nuraziza Z Al Hadar; Astija; Moh. Sabran; Bustamin; Isnainar; Mursito S. Bialangi
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20718

Abstract

This study aimed to examine the effect of a poster-assisted Think Talk Write cooperative learning model on eighth-grade students’ learning outcomes in biology at SMP Negeri 19 Palu. A quasi-experimental method was employed using a nonequivalent control group design. The study involved two classes selected through purposive sampling: Class VIII A, consisting of 31 students, served as the control group, while Class VIII C, consisting of 28 students, served as the experimental group. Data were collected using a multiple-choice achievement test. The findings showed that the poster-assisted Think Talk Write cooperative learning model had a significant effect on students’ learning outcomes in the topic of the excretory system. The Mann–Whitney U test comparing the posttest scores of the experimental and control groups yielded an Asymp. Sig. (two-tailed) value of 0.001, which was lower than the significance level of 0.05. This result indicated a statistically significant difference between the two groups after the intervention. Furthermore, the experimental group achieved a mean normalized gain of 73% (g = 0.73), which was higher than that of the control group, which achieved 55% (g = 0.55). These findings demonstrate that the poster-assisted Think Talk Write learning model significantly improves students’ biology learning outcomes. Therefore, this model may be considered an effective alternative instructional strategy for enhancing student achievement.
Microplastics in Lake Poso Sediments and Their Use as Educational Media Agreista Toripa; Fatmah Dhafir; Musdalifah Nurdin; Masrianih; Abd. Hakim Langgeng; Syech Zainal
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20727

Abstract

Microplastic pollution is an emerging environmental concern because these particles can accumulate in aquatic ecosystems but are difficult to detect visually. This study aimed to identify the morphological types and determine the density of microplastics in sediments from Lake Poso, Central Sulawesi, Indonesia, and to develop an educational poster based on the findings. A quantitative descriptive approach was applied. Sediment samples were collected from three categories of sites: an area with minimal human activity, a tourism area, and a residential area. Two sampling points were established at each site, yielding six sediment samples. At each sampling point, 200 mL of sediment was collected using the hand-collection method and analyzed at the Biology Laboratory, Faculty of Teacher Training and Education, Tadulako University. Organic matter was digested using a 10% KOH solution, after which the samples were filtered and examined microscopically. Suspected microplastic particles were classified as fibers, fragments, films, or pellets. Microplastics were detected in all sediment samples, although their density varied among sites. Fragments were the most abundant type, followed by fibers, films, and pellets. Microplastic densities were 170 particles/100 g of dry sediment at the site with minimal human activity, 281 particles/100 g at the tourism site, and 218.5 particles/100 g at the residential site. The educational poster developed from these findings was evaluated by content, design, and media experts, as well as student users, and was categorized as highly suitable for educational use. These findings indicate that Lake Poso sediments are contaminated with microplastics, potentially associated with tourism, fisheries, residential activities, and other anthropogenic inputs. Improved waste management and environmental monitoring are therefore needed to reduce microplastic contamination in the lake.
Microbiological Quality and Presumptive Enterobacter Contamination in Beef Liver Sold at Traditional Markets in Sidoarjo City Kurnia Dwi Agustin; Isnawati
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20732

Abstract

This study aimed to evaluate the microbiological quality of beef liver sold in traditional markets in Sidoarjo City by determining the total plate count (TPC) of total microorganisms and Enterobacteriaceae, as well as conducting presumptive identification of the genus Enterobacter through biochemical testing. The TPC results for total microorganisms showed that 7 of the 10 samples exceeded the maximum limit specified by the Indonesian National Standard (SNI). In addition, all 10 samples exceeded the Enterobacteriaceae limit established in SNI 9159:2023. Biochemical test results indicated that isolate B2, obtained from Porong Market, exhibited characteristics similar to those of Enterobacter cloacae, namely indole (–), H₂S (–), motility (+), methyl red (MR)    (–), Voges–Proskauer (VP) (+), citrate (+), and urease (+). The high levels of microbial contamination indicate that beef liver samples from traditional markets in Sidoarjo City did not meet the microbiological standards for safe consumption. Therefore, to obtain its nutritional benefits while minimizing the risk of microbial infection, beef liver should not be consumed raw and should be cooked thoroughly at approximately 70°C.
Microplastic Analysis in the Digestive Tract of Squid (Loligo sp.) Sold at a Traditional Market in Palu City and Its Application as an Educational Medium Ni Kadek Apriyani; Fatmah Dhafir; Abd. Rauf; Achmad Ramadhan; Moh. Sabran; Masrianih
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20735

Abstract

This study aimed to identify the morphological types of microplastics present in the digestive tracts of squid (Loligo sp.) sold at a traditional market in Palu City. A descriptive approach combining quantitative and qualitative analyses was employed. A total of 10 fresh squid specimens were randomly selected from a single vendor at Lasowani Traditional Market. The digestive tracts were isolated, digested using 10% potassium hydroxide (KOH), and incubated at 40°C for 96 h. The recovered particles were subsequently classified and counted, and microplastic abundance was calculated. Three morphological types of microplastics—fibers, films, and fragments—were detected in the digestive tracts of the 10 squid specimens. A total of 948 particles were recorded, corresponding to a mean abundance of 94.8 particles per individual. Films were the most prevalent type, accounting for 48.4% of the total particles, followed by fragments at 26.7% and fibers at 24.9%. These findings indicate that film-type microplastics were dominant in the digestive tracts of the examined squid. Based on the research findings, an educational poster was also developed and subjected to expert validation. The poster achieved a feasibility score of 82.23%, indicating that it was highly suitable, based on the validation results, for use as a learning resource in biology education.
Deep Learning in Classroom Action Research to Improve Fourth-Grade Students’ Learning Outcomes on Plants and Plant Types at Anuban Tapraya Sutan Muda; Melvariani Syari Batubara; Sahlan Tuah; Jalilah Azizah Lubis; Huda Cheyeng
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.21102

Abstract

This study aims to analyze the application of the deep learning approach in improving the learning outcomes of fourth-grade students at Anuban Tapraya School, Songkhla Province, Thailand, regarding plant-related topics and plant types. The research method used was Classroom Action Research (CAR) based on the Kemmis and McTaggart model, conducted in two cycles, each consisting of the planning, action, observation, and reflection stages. The research subjects were all 12 fourth-grade students. Data collection instruments included a learning outcome test that had been validated for validity and reliability, observation sheets, and documentation. Success was defined as individual achievement of a score of ≥75 and a class achievement rate of ≥75%. The research results show that the application of the deep learning approach can improve student learning outcomes, with the class average score increasing from 70.33 in Cycle I to 76.25 in Cycle II, and the classical mastery rate rising from 41.67% to 75%. In addition, students’ learning activities improved in terms of mindful learning, joyful learning, and meaningful learning. Thus, the deep learning approach can serve as an alternative instructional strategy to improve learning outcomes and active student engagement in science education in elementary school, although these findings are still limited to the context of a single classroom and require further, broader research.
Development of the Harmoni Smart Box Learning Media to Enhance Fifth-Grade Students’ Conceptual Understanding of Ecosystems Anisa Nurul Fiqriyah; Diki Rukmana
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.21153

Abstract

This study aimed to develop the Harmoni Smart Box Learning Media, an interactive instructional medium that integrates a three-dimensional ecosystem model, ecosystem concept cards, environmental problem cards, and student-centered exploratory activities to support students’ conceptual understanding. The study employed a Research and Development (R&D) approach based on the ADDIE model. Before classroom implementation, the product was validated by experts in science content, instructional media, pedagogy, and research instrument development. A limited field trial using a one-group pretest–posttest design was subsequently conducted with 32 fifth-grade students at SDN Tengah 03 Jakarta. Product feasibility was assessed using expert validation questionnaires, whereas students’ conceptual understanding was measured through pretest and posttest assessments. The expert validation results showed that the developed learning media achieved a very high level of feasibility across all evaluated aspects. Students’ conceptual understanding also improved after using the media, and the Wilcoxon signed-rank test revealed a statistically significant difference between the pretest and posttest scores (p < 0.05). These findings indicate that the Harmoni Smart Box Learning Media is highly feasible for use in elementary science instruction and shows promising potential to support students’ conceptual understanding in a limited classroom setting. Nevertheless, further studies involving larger and more diverse samples, as well as control groups, are required to evaluate its instructional effectiveness more comprehensively.
Maternal Health Literacy and Exclusive Breastfeeding Intention: A Theory of Planned Behaviour Study in Antenatal Care Abiola Adiat Omokhabi
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.21181

Abstract

This study examined the joint and independent contributions of maternal health literacy, attitudes toward exclusive breastfeeding, subjective norms, and perceived behavioural control to exclusive breastfeeding intention among pregnant women attending antenatal care in Ibadan North Local Government Area, Nigeria. The study extended the Theory of Planned Behaviour by incorporating maternal health literacy as an additional cognitive predictor. A facility-based cross-sectional survey was conducted among 181 third-trimester pregnant women recruited through convenience sampling from three Primary Health Centres in Idi-Ogugu (Agodi-Gate), Agbowo, and Oke-Apon (Yemetu). Data were collected from July to December 2025 using a modified Maternal Health Literacy and Pregnancy Outcome Questionnaire, Theory of Planned Behaviour-based measures, and the Infant Feeding Intentions Scale. Descriptive statistics and multiple linear regression were used for analysis. Participants had a low level of maternal health literacy, with an overall weighted mean of 1.98. The regression model was statistically significant (R = 0.455, R² = 0.207, adjusted R² = 0.189, F(4,176) = 11.467, p < 0.001) and explained 20.7% of the variance in exclusive breastfeeding intention. Maternal health literacy (β = 0.345, p = 0.002) and attitudes toward exclusive breastfeeding (β = 0.210, p = 0.033) were significant positive predictors, whereas subjective norms and perceived behavioural control were not significant independent predictors. These findings highlight the importance of strengthening maternal health literacy and promoting favourable breastfeeding attitudes through accessible, culturally appropriate antenatal education.
Association of Body Mass Index (BMI) and Urinary Glucose Levels with Urinary Tract Infections I Putu Wira Janarda; Pebrian Jauhari; Sabariah; M. Ashabul Kahfi Mathar
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 3 (2026): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i3.19115

Abstract

This study aimed to investigate the association of body mass index (BMI) and urinary glucose levels with urinary tract infection (UTI) among patients at West Nusa Tenggara Provincial Hospital. A cross-sectional study was conducted at the hospital in October 2025. A total of 104 participants were selected using simple random sampling. BMI data were obtained from medical records based on body weight and height measurements recorded using a weighing scale and stadiometer. Urinary glucose data were retrieved from laboratory records and measured using a Dialab AutoLyser. Data were analyzed using univariate analysis, Spearman’s rank correlation for bivariate analysis, and ordinal logistic regression for multivariable analysis. The results showed a significant positive correlation between BMI and UTI category (correlation coefficient = 0.286; p = 0.003). Urinary glucose levels were also significantly and positively correlated with UTI category (correlation coefficient = 0.333; p = 0.001). The multivariable analysis yielded a Nagelkerke R² value of 0.186. BMI was significantly associated with UTI category (p = 0.032; OR = 1.58), as were urinary glucose levels (p = 0.003; OR = 2.80). In conclusion, BMI and urinary glucose levels were significantly associated with urinary tract infection categories among patients at West Nusa Tenggara Provincial Hospital.

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