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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,493 Documents
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Identifying the Effects of Online Gaming Addiction on Students’ Academic Behavior in Biology Classes at SMA Negeri 5 Palu Nikmah Nur Qoiriyah; Samsurizal `M. Suleman; Syech Zainal; Lilies; Moh. Sabran; Raya Agni
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20459

Abstract

This study aims to identify the impact of online game addiction on students’ academic behavior in Biology learning at SMA Negeri 5 Palu. A descriptive qualitative method was employed. The research subjects consisted of four Grade X Merdeka 2 students, selected purposively, and a Biology teacher. Data were collected through interviews and documentation. This study addresses a gap in previous literature by examining students’ subjective experiences regarding the effects of online game addiction on academic behavior in Biology classes at SMA Negeri 5 Palu. The findings indicate that online game addiction affects three aspects of students’ academic behavior: decreased classroom participation, lack of discipline in submitting assignments, and potential disruption of academic achievement. Among these aspects, students’ lack of discipline in completing assignments emerged as the most prominent and evident impact. This was supported by the consistency of interview responses from all informants, the normalization of rule violations, and confirmation from the Biology teacher that, without reminders, assignment submission could be delayed for up to one week. It can be concluded that interventions aimed at improving students’ discipline, time management, and daily limits on online gaming are needed through collaboration among schools, teachers, and parents.
Types and Abundance of Microplastics in African Catfish (Clarias gariepinus) from Sumber Agung Village, Parigi Moutong Mudrikah Tus Sa'diah; Fatmah Dhafir; Masrianih; Sutrisnawati; Bustamin; Rafiqa
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20493

Abstract

This study aimed to determine the types and abundance of microplastics in African catfish (Clarias gariepinus) collected from aquaculture ponds and a river in Sumber Agung Village. A descriptive research design was employed using both qualitative and quantitative approaches. A total of 10 catfish aged approximately two months were used as samples, consisting of five individuals from aquaculture ponds and five individuals from the river. Digestive tract samples were digested using 10% KOH solution for 96 hours to separate microplastic particles, followed by filtration, identification, and particle counting. The results showed that microplastics were detected in all catfish samples. Three types of microplastics were identified: fibers, films, and fragments. The abundance of microplastics in catfish from aquaculture ponds was 103.6 particles/individual, which was higher than that observed in catfish from the river, at 57.6 particles/individual. Fragments were the dominant microplastic type in pond-derived catfish, whereas fibers predominated in river-derived catfish. Differences in microplastic abundance were influenced by the characteristics of the aquatic environment, with ponds having limited water circulation that may facilitate microplastic accumulation. These findings indicate that catfish have the potential to serve as bioindicators of microplastic pollution in both aquaculture systems and natural freshwater environments. However, microplastic identification in this study was based solely on microscopic observation; therefore, the polymer composition of the detected microplastics could not be confirmed.
Morphometric Characteristics of Freshwater Shrimp in Lotic and Lentic Habitats of Ogan Ilir Regency, South Sumatra Stilaf Terbit Galih Walsandy; Arum Setiawan; Kodri Madang
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20603

Abstract

This study aimed to analyze the length–weight relationship and morphometric variation of three freshwater shrimp species from lotic and lentic habitats in Ogan Ilir Regency, South Sumatra, Indonesia. The study was conducted from November 2025 to May 2026 using purposive sampling at six aquatic stations, comprising three lotic and three lentic habitats. A total of 300 shrimp specimens were collected using baited traps (bubu) and hand nets. The observed parameters included total length and body weight. Length–weight relationships were analyzed using the allometric equation W = aLᵇ, whereas morphometric variation was evaluated using Principal Component Analysis (PCA). The results showed that Palaemon carcinus exhibited positive allometric growth in both lotic (b = 5.805) and lentic habitats (b = 6.963). Similarly, Macrobrachium rosenbergii showed positive allometric growth in lotic (b = 8.230) and lentic habitats (b = 3.973). In contrast, Macrobrachium nipponense demonstrated positive allometric growth in lotic habitats (b = 4.960) but negative allometric growth in lentic habitats (b = 0.899). PCA revealed that most morphometric variation was explained by the first principal component (PC1), indicating a strong association between total length and body weight. These findings suggest that habitat type, particularly differences between lotic and lentic aquatic systems, influences growth patterns and morphometric variation in freshwater shrimps in South Sumatra.
Potential of Bacillus spp. Isolates as Biological Control Agents Against Rhizoctonia solani, the Causal Agent of Sheath Rot in Maize (Zea mays L.) Salma Alfiani Nadiva; Yenny Wuryandari; Tri Mujoko
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20689

Abstract

This study aimed to evaluate the effectiveness of laboratory-collected Bacillus spp. isolates against Rhizoctonia solani, the causal agent of maize sheath blight, under both in vitro and in vivo conditions, as available information on their efficacy remains limited. The in vitro assay was conducted using the dual culture method with five Bacillus spp. isolates, namely Bcz-14, Bcz-16, Bcz-20, Bcz-21, and Bcz-30, to assess their inhibitory activity against R. solani. The results showed that all isolates were able to inhibit pathogen growth, although the percentage of inhibition varied among isolates. Isolate Bcz-14 exhibited the highest mean inhibition, while isolates Bcz-16 and Bcz-21 were selected for the in vivo assay based on a combination of mean inhibitory activity and consistency across replications. The in vivo assay was conducted using the two selected isolates with different application times, namely seven days before planting and at planting. The results indicated that treatment B1T1, consisting of Bcz-16 applied seven days before planting, showed a greater tendency to suppress disease development, as indicated by a longer incubation period and the lowest disease intensity of 17.78%. These findings suggest that isolate Bcz-16 has potential for further development as a biological control candidate for maize sheath blight. However, further evaluation under field conditions is required to confirm its effectiveness.
Analysis of Environmental Literacy among Students at MAN 2 Tuban A. Farid Rohmatulloh; Vivi Novianti; Mimien Henie Irawati Al Muhdhar
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.18117

Abstract

This study aimed to analyze the environmental literacy level of students at MAN 2 Tuban based on four aspects: ecological knowledge, cognitive skills, environmental attitudes, and environmental behavior. A quantitative descriptive research design was employed. The sampling technique used was simple random sampling, involving 66 eleventh-grade students as the research sample. Data were collected through tests and surveys. The research instruments consisted of multiple-choice questions to assess ecological knowledge and cognitive skills, and an environmental literacy questionnaire adapted from the Middle School Environmental Literacy Survey to measure students’ environmental attitudes and behavior. The findings indicated that students’ ecological knowledge and cognitive skills were lower than their environmental attitudes and behavior. Overall, 77.27% of students were categorized as having a moderate level of environmental literacy, while 22.73% were categorized as having a high level. The mean scores for each aspect were 57 for ecological knowledge, categorized as fair; 51 for cognitive skills, categorized as fair; 69 for environmental attitudes, categorized as high; and 61 for environmental behavior, categorized as high. These results reveal an imbalance between students’ conceptual understanding and their environmental attitudes and behavioral tendencies. Therefore, learning strategies that place greater emphasis on strengthening cognitive aspects are needed to ensure alignment between students’ knowledge, attitudes, and behavior toward the environment.
Sediment Carbon Stocks and CO₂ Sequestration Capacity in the Bagek Kembar Mangrove Essential Ecosystem Area, West Lombok Eni Suyantri; Gito Hadiprayitno; Didik Santoso; Karnan; M. Liwa Ilhamdi; Pahrurrozi
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.19485

Abstract

This study aimed to analyze and compare sediment organic carbon stocks, bulk density, and estimated carbon dioxide (CO₂) sequestration in natural and revegetated mangrove areas within the Bagek Kembar Mangrove Essential Ecosystem Area (KEE), Sekotong, West Lombok. Sediment samples were collected to a depth of 100 cm and analyzed using the Loss on Ignition (LOI) method. The results showed that bulk density ranged from 1.19 to 1.50 g/cm³ and tended to decrease with increasing sediment depth. Sediment organic carbon stocks were higher in the natural mangrove area (591.49 Mg C/ha) than in the revegetated mangrove area (575.39 Mg C/ha), with the greatest carbon accumulation observed at depths of 61–100 cm. This difference indicates that natural mangroves have a slightly greater capacity for carbon accumulation than revegetated areas, likely due to more stable stand structure and sediment dynamics. Estimated CO₂ sequestration followed a similar pattern, reaching 2,170.55 Mg CO₂/ha in the natural mangrove area and 2,111.68 Mg CO₂/ha in the revegetated mangrove area. These findings provide a scientific contribution by addressing data gaps on sediment organic carbon stocks and estimated CO₂ sequestration in mangrove ecosystems in Lombok. They also strengthen the basis for conservation- and restoration-oriented management to support climate change mitigation in coastal ecosystems.
Early Colony Growth of the Stingless Bee Heterotrigona itama After Transfer to Cultivation Boxes in Three Types of Anthropogenic Landscapes Elpe Bibas; Fitriyan Kurnia
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.19645

Abstract

This study aimed to evaluate changes in colony weight during the first ten weeks after transfer to cultivation boxes and to assess nest-external activity (foraging) across mixed-garden, suburban, and urban landscapes. A total of 27 colonies were observed over ten weeks. Colony growth was measured based on changes in colony weight, whereas worker activity was recorded using video observations. The data were analyzed using Linear Mixed Models (LMMs) and Generalized Linear Mixed Models (GLMMs). The results showed that time since placement in the cultivation boxes (LMM: χ² = 17.17; p < 0.001) and its interaction with landscape type (LMM: χ² = 22.13; p < 0.001) had significant effects on changes in colony weight. Colonies in mixed-garden landscapes showed more progressive weight gain, reaching 654.82 ± 81.96 g in week 8 and 737.20 ± 108.37 g in week 10, whereas colonies in suburban and urban landscapes tended to remain stagnant. Landscape type had no significant effect on foraging activity (p > 0.05). In contrast, time had a significant effect on the number of workers carrying nectar (GLMM: χ² = 9.30; p = 0.0023) and resin (GLMM: χ² = 10.23; p = 0.0014). Nectar-carrying activity increased and peaked between weeks 8 and 9, whereas resin-carrying activity was higher during the early weeks and declined in subsequent weeks. Under the specific locations and observation period of this study, mixed-garden landscapes provided more favorable conditions for the early growth of H. itama colonies than suburban and urban landscapes.
Literature Review on the Potential of Pleurotus spp. Fungal Mycelium as a Biodegradable Packaging Material for Styrofoam Replacement Adhania Andika Prayudanti; Dwirini Kartikasari; Indra Wirawan
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.19818

Abstract

This literature review aimed to: (1) identify edible fungal species with potential applications as mycelium-based packaging materials; (2) compare mycelial characteristics in terms of substrate compatibility, mechanical properties, thermal stability, water absorption, and food safety; and (3) recommend the most suitable species for the development of biodegradable food packaging. A systematic scoping review was conducted following the framework of Arksey & O’Malley (2005) and PRISMA-ScR guidelines. Literature searches were performed in Scopus, Web of Science, ScienceDirect, and Google Scholar for publications from 2015 to 2024 using the keywords mycelium composite, mycelium-based foam, Pleurotus packaging, and biodegradable packaging fungi. Of the 312 articles initially identified, 47 met the inclusion criteria, namely empirical studies that assessed at least two material parameters and were published in English or Indonesian. The findings indicate that Pleurotus ostreatus exhibits several advantages, including rapid colonization time (7–14 days), suitable mycelium density (25–75 kg/m³), tensile strength (0.18–0.43 MPa), low thermal conductivity (0.03–0.07 W/m·K), and a high biodegradation rate in soil (60–90% per week). Pleurotus citrinipileatus demonstrated slightly lower mechanical performance but greater adaptability to mixed substrates. Both species are classified as generally recognized as safe (GRAS) by the FDA and do not produce significant levels of mycotoxins under standard cultivation conditions. Overall, P. ostreatus shows strong potential as a biodegradable packaging material to replace expanded polystyrene (EPS). However, industrial-scale experimental validation, food contact migration testing, and production standardization remain essential before commercial application.
The Urgency of Transforming Indigenous Knowledge into Contextual Science Practicum Activities: A Preliminary Study among Preservice Science Teachers and Science Educators Ahmad Aris Arifin; Agus Ramdani; A. Wahab Jufri; Satutik Rahayu
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20281

Abstract

This preliminary study aimed to examine the need for validated instructional support in transforming Sasambo indigenous knowledge into contextual science practicum activities for preservice and in-service science teachers. A descriptive survey design was employed involving 100 respondents, consisting of 76 preservice science teachers from Biology, Physics, and Chemistry Education programs and 24 in-service science teachers. These groups were selected to obtain complementary perspectives: preservice teachers represent future science educators who require contextual pedagogical preparation, whereas in-service teachers provide practical insights from classroom practice. Data were collected using an online questionnaire comprising nine closed-ended items related to the availability of science learning resources, the integration of Sasambo local wisdom into science learning, and the need for validated instructional support. The questionnaire was reviewed by experts to ensure content validity and tested for internal consistency to confirm instrument reliability. Descriptive statistics, including frequencies and percentages, were used to analyze the data. The findings revealed substantial gaps in current science learning support. Most respondents perceived science learning resources and portals as limited (82%), reported difficulty finding complete instructional materials (83%), and experienced challenges in accessing reliable and expert-validated resources (87%). The integration of Sasambo local wisdom into science learning was also limited, as 81% of respondents had never connected Sasambo local wisdom with science learning, 82% had difficulty integrating it into science content, and 87% had never implemented Sasambo-based ethno-practicum activities. In contrast, respondents expressed strong support for the development of an integrated learning platform: 98% indicated the need for a portal providing complete and validated instructional materials, 93% agreed that a Sasambo ethno-practicum portal would support innovative learning design, and 96% emphasized the need for reconstructed ethno-Sasambo data. These findings highlight the urgent need to develop validated, contextual, and accessible ethno-practicum learning support to facilitate the transformation of indigenous knowledge into meaningful science practicum activities.
Effectiveness of Antagonistic Agents from Several Bacillus spp. Isolates against Anthracnose Disease (Colletotrichum capsici) in Chili Pepper Fruit (Capsicum annuum L.) Aullyvia Putri Nabiella; Yenny Wuryandari; Herry Nirwanto
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20692

Abstract

This study aimed to evaluate the antagonistic activity of selected Bacillus spp. isolates against Colletotrichum capsici and determine the most effective isolate and cell density for suppressing anthracnose in red chili pepper. The study was conducted under in vitro and in vivo conditions. The in vitro assay tested five Bacillus spp. isolates, namely Bcz-14, Bcz-16, Bcz-20, Bcz-21, and Bcz-30, at cell densities of 10⁹ and 10¹⁰ CFU/mL. The three most effective treatments from the in vitro assay were subsequently evaluated under in vivo conditions. Data were analyzed using SPSS version 24 through analysis of variance (ANOVA) at a 5% significance level, followed by Tukey’s honestly significant difference (HSD) test at 5% when significant differences were detected. The results showed that B1K1 treatment (Bcz-14 at 10⁹ CFU/mL), with an inhibition zone of 5.83 mm; B5K1 treatment (Bcz-30 at 10⁹ CFU/mL), with an inhibition zone of 5.50 mm; and B2K1 treatment (Bcz-16 at 10⁹ CFU/mL), with an inhibition zone of 4.16 mm, exhibited the most stable inhibitory effects against C. capsici growth in vitro. The in vivo assay demonstrated that B1K1 treatment (Bcz-14 at 10⁹ CFU/mL) was the most effective treatment, resulting in a disease intensity of 26.25% and a control efficacy of 51.16%.

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