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INDONESIA
Hemera Zoa
Published by Institut Pertanian Bogor
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Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
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Articles 391 Documents
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PCS-6 Preparation and Application of Nano Chitosan Particles as Adjuvan in Rabies Vaccination Based on Anti-Idiotype Antibody Sayu Putu Yuni Paryati; K Hasan; S Ramadhanti
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Rabies is a fatal disease to humans and animals, but can be controlled by prophylaxis administration before being exposed. One type of vaccine that can be utilized is an anti-idiotype antibody vaccine originating from IgY purification of chicken egg yolk. The use of vaccine additives in the form of adjuvants is very important to improve the effectiveness of vaccines [1].Chitosan is non-toxic, easily synthesized, safe to use and able to induce an immune response by activating macrophages. Chitosan can modulate dendritic cell maturation so that it can induce interferon interactions and stimulate activity of T lymphocytes and B lymphocytes. Chitosan can be made in the form of chitosan nanoparticles that can be used to deliver drugs and vaccines through inhalation pathways, orally, intravenously and as non-viral gene delivery vectors. In the oral delivery of chitosan nanoparticles are able to overcome the problem of solubility, protect the drug from enzymatic degradation, controlled release, and extend the time of action in the bloodstream through ionic mechanisms with mucin. Inhalation of chitosan 0.5mg/ml with influenza vaccine produces a good response [2-4].In this work, the preparation of chitosan nanostructures and their application as adjuvan in antibody anti-idiotype rabies vaccination were reviewed.
PCS-7 Lead Detection in Blood and Liver of Cattle sold in the Traditional Market of Denpasar City I Ketut Berata; Ni Nyoman Werdi Susari; I Wayan Sudira; I Made Kardena
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Lead contamination in many foods is reported to endanger consumers' health. Beef as one of the food of animal origin can be contaminated by lead mainly due to the maintenance of cattle in contaminated areas. Balinese cattle that are kept in urban landfills are reported to be contaminated with high levels of lead in the blood and in other tissues with lower levels [4]. The threat of human health from eating foods that contain leads can cause anemia because leads can substitute iron in hemoglobin [2]. Lead poisoning can also cause malfunction of various organs such as liver, kidney [1], lungs, spleen [7] and brain [9] in the form of cognitive impairment [5]. The high threat due to contamination of leads to health, then the meat health check should also be made on the presence of leads. One of the inspection efforts on the presence of lead contamination in beef, then the place of sale in the traditional market is a good location for sampling examination.
PCS-8 Oyster Shell Powder as Alternatives Macromineral for Synthetic Testosterone Pudji Astuti; Claude Mona Airin; Alfarisa Nururrozi; . Harimurti
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Oyster is one of seafood produced in waters, especially in eastern Indonesia. During this time, oyster is known as aphrodisiac agents, an agent that can increase sexual appetite because it can remind testosterone levels and very high nutrient content of zinc, vitamin A iron, calcium, and selenium are also Vitamins A and Vitamin B12. Therefore, the oyster is known as the mineral's potent testosterone-boosting abilities (Matsuda et al., 2003). Zinc is also very important, it can be used as a stimulant of enzymes, hormones and the immune system.Due to the abundance of oysters in Indonesia, only meat of oyster is consumed, while the existing shells are never used to consume and just thrown away. Therefore, a research plan will be made on the use of oyster shell waste, as a precursor of testosterone in Rat (Rattus norvegicus). Since many kinds of oyster, it would be compared among three kinds of oyster shell namely Kerang darah (Anadara granosa), Kerang hijau (Perna viridis) and Kerang keong (Telescopium telescopium).Based on function, Zn inhibits aromatase enzyme which convert estradiol to testosterone as a consequence, testosterone levels will increase (Michell et al. 2012).The purpose of this study were to determine the highest content of Zn among the three types of oyster shell; 2. To measure testosterone levels in rat after given shell powder containing highest Zn.
PCS-9 Effect of Freeze Drying of Follicular Fluid from Waste of Aceh Cattle Ovary on the Estradiol Concentration M Adam; G Gholib; M Hamdan; M Syafruddin; Hafizuddin Hafizuddin; H Alief
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Aceh cattle are one of the many cow’s species that are widely preserved and distributed in Aceh Province. This species is the result of a cross between Bos sundaicus and Bos indicus and has been designated as a native Indonesian class of cattle in 2011 by the Minister of Agriculture of Indonesia through the decision of the Minister of Agriculture number 2907 / Kpts / OT.140 / 6/2011 [1]. Aceh's cattle population in 2017 in total was 627,629 species [2]. Slaughtering the cows results in the obtaining of byproduct or waste such as ovaries. The ovarium of female cows is one of the complement results of animal slaughter house which is infrequently used. In fact, the ovaries comprise follicles which contain many active compounds inside the follicular fluids such as insulin-like growth factor I (IGF-I), follicle stimulating hormone (FSH), luteinizing hormone (LH), estrogen and progesterone [3]. Freeze drying/lyophilization is one of the preservative process which are generally used in food and non-food processing. This study was conducted to determine the effect of these methods on the concentration of estrogen in follicular fluid.
PCS-10 The Features of Seminiferous Tubule Cells in Rat Testicular Dysfunction Induce by Secretome Based On Cytoskeletal Protein Profile Surya Agus Prihatno; Irma Padeta; Arinda Devi Larasati; Betty Sundari; Annisa Hidayati; Yuda Heru Fibrianto; Teguh Budipitojo
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Secretome is a factor found in the stem cell culture medium. This factor may repair the tissues of the organs that were damaged by various degenerative disorders1. A previous study has reported that secretome derived from the fetal human umbilical cord mesenchymal stem cell (HUC-MSC) may be an effective regenerative agent for β-cell pancreatic regeneration in Type 1 diabetes mellitus and for skin regeneration in incisional and burn wound healing.The administration of cisplatin as a chemotherapeutic agent is limited because it causes side effects, such as reproductive toxicity and progresses to testicular dysfunction2. As a degenerative disorder, testicular dysfunction is characterized by failure in the synthesis of reproductive hormones and spermatogenesis. Cisplatin-induced testicular cell damage would promote vimentin and cytokeratin localization in the spermatogenic, Sertoli, and Leydig cells.Studies on the use of secretome in promoting the recovery of various degenerative disorders and its effects on the spermatogenesis of rats with cisplatin-induced testicular dysfunction have not been conducted. The aim of this stud was to investigate the effect of secretome derived from HUC-MSC on cisplatin-induced testicular dysfunction in rats.
PCS-11 Mesenchymal Stem Cell-Conditioned Medium Improve the Recovery of Pancreatic α and β Cells in Type 1 Diabetes Mellitus Dian Bekti Hadi Masithoh; Yuda Heru Fibrianto; Marla Anggita; Widagdo Sri Nugroho; Teguh Budipitojo
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Mesenchymal stem-cell is a pluripotent cells that can be differentiated into many kinds of cells (1) and have abilities to differentiated into mature cell, secreted cytokines, and releasing many kinds of protein functioned in body body regulation (2). However, the therapeutic effect of stem cell arise from their secreted factor such as growth factors, cytokines, chemokines, and metabolits which acts as biologic regulator in the autocrine and paracrine body function (3). Secreted factors of mesenchymal stem cells known as secretome or mesenchimal stem cell conditioned medium (MSC-CM) have therapeutic effect for antiapoptosis, angiogenic, immunomodulatoric, and chemoattraactive activity (4). Mesenchymal stem cell conditioned medium used as the replacement agent for cell therapy because it contains no cells and save for used in different individual (5).Type 1 diabetes mellitus (DMT1) is a disorder characterized by destruction of the insulin-producing pancreatic β-cell that progressively leads to insulin deficiency and resultant hyperglycemia. Insulin deficiency can lead to progressive metabolic derangement, with worsening hyperglycemia, ketoacidosis, starvation, and death (6). Glucagon is a hormone produced by pancreatic α cells. Glucagon releases by the pancreas to raise the concentration of glucose in the bloodstream when the concentration of glucose in the bloodstream too low (7). Glucagon worked in opposite with insulin, which lowers the extracellular glucose level, while insulin works to increase the extracellular glucose level (8). The aim of this study was to investigate the role of MSC-CM on the structural and functional regeneration of pancreatic α and β cells in Wistar rat (Rattus norvegicus) induced with type 1 diabetes mellitus. The datas in this study will completes the information about the effect of MSC-CM on pancreatic cells regeneration, which has been presented in previous study (9).
AEVI-15 Investigasi Outbreak Avian Influenza (AI) pada Peternakan Puyuh di Kecamatan Tenayan Raya Kota Pekanbaru Tahun 2018 . Hamria
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Kejadian AI di Indonesia telah terjadi di beberapa Provinsi. Provinsi Riau khususnya Kota Pekanbaru adalah daerah endemis AI, dan dilaporkan sejak tahun 2008. Pada Tahun 2014, kejadian penyakit Avian Influenza di Kota Pekanbaru tidak lagi terjadi wabah melainkan hanya bersifat sporadis. Tercatat beberapa kasus laporan kematian burung puyuh dari masyarakat maupun peternak sejak tahun 2014, dan hasilnya adalah negatif Avian Influenza.Tujuan kegiatan adalah menyelidiki kejadian kematian burung puyuh di Kelurahan Tuah Negeri Kecamatan Tenayan Raya dan melakukan pengumpulan data epidemiologis sehingga diketahui penyebab/sumber penularan kematian burung puyuh di kelurahan tersebut, mengidentifikasi faktor resiko dan menentukan langkah-langkah pengendalian.
AEVI-16 Investigasi Wabah Anthraks di Kabupaten Pinrang Propinsi Sulawesi Selatan Tahun 2016 I G A Priyamanaya
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Anthraks adalah penyakit menular yang biasanya bersifat akut atau perakut pada berbagai jenis ternak (pemamah biak, kuda, babi dan sebagainya), yang disertai dengan demam tinggi dan disebabkan oleh Bacillus anthracis. Biasanya ditandai dengan perubahan-perubahan jaringan bersifat septisemia, timbulnya infiltrasi serohemoragi pada jaringan subkutan dan subserosa, disertai dengan pembengkakan akut limpa (Tarumingkeng 2014).Insidensi kasus di provinsi Sulawesi Selatan sebelum tahun 2015 hanya ada 5 Kabupaten/kota yang endemis Anthrax diantaranya Kota Makassar,Kabupaten Maros,Gowa,Takalar dan Bone, dengan hewan rentan yang tertular : sapi,kerbau,kuda dan babi dan kesemuanya menimbulkan kematian pada ternak dan penularan ke manusia dalam bentuk anthrax kulit. Kabupaten Sidrap menjadi tujuan berikutnya setelah anthrax mewabah di Kabupaten Bone tahun 2014 dengan adanya kasus di awal tahun 2015 dan akhirnya dengan lalu lintas ternak yang tidak terkontrol kabupaten Pinrang berpeluang menjadi destinasi berikutnya wabah antraks.Tujuan kegiatan adalah melakukan penyidikan kejadian kematian sapi di Desa Malimpung Kecamatan Patampanua Kabupaten Pinrang, melakukan pengumpulan data epidemiologis,penanganan ternak sakit, pengambilan spesimen di lapangan, dan untuk mengetahui penyebab kematian Sapi di Kabupaten Pinrang
AEVI-17 Investigasi Outbreak Anthrax di Kabupaten Kulon Progo Tahun 2017 Indarto Sudarsono; Bagoes Poermadjaa; Ully Indah Apriliana; Anton Handoko; . Yuriati
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Pada tanggal 10 Januari 2017 Balai Besar Veteriner Wates mendapatkan laporan dari Dinas Kesehatan Kabupaten Kulon Progo tentang adanya kasus beberapa orang sakit kulit dengan gejala luka dengan bagian tengah berwarna kehitaman, dan ada bagian yang melepuh, dugaan sementara gigitan serangga tomcat. Dengan informasi tersebut BBVet Wates langsung membentuk tim untuk kunjungan ke lapangan. Tujuan untuk mengetahui penyebab dari penyakit tersebut. Metode melakukan kunjungan langsung ke lokasi kasus, pengambilan sampel dan pengujian laboratorium. Hasil dan pembahasan bahwa orang yang pernah terkena kasus penyakit kulit tersebut sejumlah 14 orang di 3 dusun yakni, Dusun Ngaglik, Penggung dan Ngroto, dusun yang sempat dikunjungi adalah dusun Ngaglik, semua penderita di desa Ngaglik telah sembuh walaupun meninggalkan bekas luka. Hasil wawancara dengan Kepala Dukuh Ngaglik dan beberapa orang penderita diperoleh data kematian ternak sejak bulan Nopember 2016 hingga tanggal 10 Januari 2017 sejumlah 1 ekor sapi dan 13 ekor kambing. Gejala kambing sebelum mati beberapa tidak diketahui, beberapa menunjukkan gejala kejang. Hasil kultur anthrax dari sampel tanah di 2 tempat pemotongan paksa dan sampel sisa daging kambing dari refrigerator dengan nomor epid 0032/01/2017, hasil uji tanah dengan kultur dan PCR menunjukkan hasil positif Bacillus anthracis, sedangkan hasil uji drift daging menunjukkan kultur negatif Bacillus anthracis, akan tetapi dengan uji PCR positif anthrax patogen.
AEVI-18 Investigasi Outbreak Penyakit Jembrana di Kecamatan Bayung Lincir, Kabupaten Musi Banyu Asin Joko Siswanto; E Yulianti; T Guntoro
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Penyakit Jembrana pada sapi Bali disebabkan oleh virus penyakit Jembrana yang termasuk dalam kelompok retrovirus berdasarkan pada aktivitas reverse transcriptase. Virus Jembrana merupakan virus RNA dengan utas tunggal, berbentuk icosahe-dral dengan panjang basa 7732 pasang basa (pb) dan bersifat patogen hanya pada sapi Bali. Gejala umum ternak yang terserang penyakit Jembrana adalah demam tinggi, lymphadenopathy, lymphopenia, keringat darah dan mucus yang berlebihan pada mulut dan hidung. Kematian ternak akibat JDV terjadi pada 1 atau 2 minggu setelah infeksi (Indriawati dan Ridwan, 2013).Pada tanggal 22-25 Mei 2017, telah dilakukan kegiatan investigasi kematian sapi bali suspect Jembrana di Kecamatan Bayung lencir Kabupaten Musi Banyuasin Provinsi Sumatera Selatan.  Kegiatan dilakukan di desa Pale Gading, Simpang Mendis,Kali Weru,Sindang Marga ,Desa Banjar Jaya dan Desa Sidomulyo. Kegiatan ini dilakukan atas permohonan investigasi oleh Dinas Pertanian dan Peternakan kabupaten Musi Banyuasin yang kemudian ditindaklanjuti oleh Balai veteriner Lampung dengan mengeluarkan surat tugas Kepala Balai Veteriner Lampung No. 19009/TU.040/F5.C/05.2017 tanggal 19 Mei  2017, dalam rangka pelaksanaan kegiatan Investigasi Penyakit Hewan Menular Sapi di Kabupaten Musi Banyuasin. Investigasi ini bertujuan untuk mengumpulkan data epidemiologi dan pengambilan spesimen dalam peneguhan diagnosa di desa Pale Gading, Simpang Mendis, Kali Weru,Sindang Marga, Desa Banjar Jaya dan Desa Sidomulyo   Kecamatan Bayung Lencir  Kabupaten Musi Banyuasin  Propinsi Sumatera SelatanTujuan kegiatan ini melakukan penelusuran kasus kematian, mengetahui faktor penyebab, upaya komunikasi resiko dan rekomendasi langkah pengendalian

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