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Hemera Zoa
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Articles 391 Documents
PAT-3 In Vitro Growth Inhibition Activities of Natural (nCaIFN) and Recombinant (rCaIFN) Canine Interferons on Three Different Tumor-Derived Cell Lines Bambang Pontjo Priosoeryanto; Rizki Rostantinata; Eva Harlina; Lina Noviyanti
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Recently, from many kinds of diseases one could be counted is a tumor disease. Tumor is a degenerative disease that involved many biological pathways within the host. A tumor or neoplasm can be defined as a disturbance of growth characterized by excessive, abnormal and uncontrolled proliferation of transformed or altered cell(s) at  one or more primary points within the host, and frequently at  one or more metastatic sites (Priosoeryanto, 1994).The treatment of tumor is mostly by medical surgery which usually combined with chemotherapeutic agent(s), unfortunately the using of chemotherapeutic agent can develop a seriously side effect to the treated-patients. Biological substances like interferon (IFN) known as anti-virus agent is also one of promising candidate for treating or preventing of tumor disorders.Feline and canine squamous cell carcinoma is one of tumor type that often found in cat and dog, and mostly develop to a very aggressive disorder. The using of in vitro cell culture is a way to avoid the use of live animals on the study of tumors especially the study of antitumor agent due to can mimic the in vivo condition.  The aim of the present study is to examine the growth inhibition activity of natural and recombinant canine interferon in order to find the suitable biological substances for combating tumor disorder especially in the field of veterinary medicine and also as an information for the development of tumor treatment in the human medical side.
PAT-4 Histopathological Study of Mice (Mus musculus) Digestive Organs Treated with Alkali pH Water Sri Estuningsih; Vetnizah Juniantito; Yenny Rakhmawati
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Water is an important part of the body. As much as 50-60% of an adult's body weight consists of water. Water functions in the body include as a means of transporting substances, regulating body temperature, regulating body pH, forming body structures, solvents for the body's chemical reactions, and helping the body's mechanical functions, such as lubrication (Insel et al. 2004; Asamadi 2008). In the event of water deficiency, the body will become dehydrated which able to cause cell death to individual death (Stanfield and Hui 2008). Recently, there are alkaline pH drinking water products that are commercially popular that have pH around 8-10 (alkaline water). Some parties claim that alkaline water can help neutralize the level of acidity (pH) of blood due to free radicals, while also having micro cluster technology that can increase oxygen solubility. According to Shirahata et al. (2012) alkaline water is beneficial for health because it can suppress oxidative stress.This study aims to know the effect of alkaline pH drinking water reviewed through histopathological studies of the digestive organs of mice (stomach, intestine, and pancreatic exocrine glands).
PAT-5 Update: Q Fever in Indonesia Agus Setiyono; Elok Puspita Rini; Handayu Untari; Eko Prasetyo Nugroho
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Q Fever is zoonotic disease caused by Coxiella burnetii, an intracellular obligate and negative Gram bacterium with pleomorphic shape (Kaplan and Bertagna 1955). Centers for Disease Control and Prevention (CDC) classify Coxiella burnetii as potential bioterrorism agent within B rank for its ability and characteristics (CDC 2013).The main reservoir animals for Q fever are ruminants (Maurin and Raoult 1999). Q fever infection whether in animal or human generally occur through inhalation, vector bites, or through oral ingestion—the last two routes are considered as secondary routes (Angelakis and Raoult 2010). Clinical symptoms of Q fever in both animal and human generally asymptomatic. Q fever can cause abortion in the third trimester of pregnancy and pneumonia in ruminants. Whether in human, acute Q fever can cause flu like syndrome and can develop into hepatitis, endocarditis, and for some severe chronic case, it caused death (Fournier et al. 1998). Office international des epizooties (OIE) classify Q fever into a re-emerging disease group (OIE 2010). Based on OIE data in 2012, the distribution of Q fever in animals occur in almost all country in the world including ASEAN (OIE 2012).Q fever was first discovered in Australia in 1935 (Kaplan and Bertagna 1955). Q fever disease transmission in animal occurs almost in every country in the world. Based on OIE data, Indonesia was classified as no information region (OIE 2012). World Health Organization (WHO) reported that Q fever was first found in cow serologically in Indonesia in 1953 (Kaplan and Bertagna 1955). Q fever in ruminants was again reported by Indonesian researcher between 2006 to 2015 in Bali, West Java, Jakarta, and Medan (Mahatmi et al. 2007; Setiyono et al. 2008; Nasution et al. 2015).Positive Q fever result was obtained especially from ex imported cows, thus it was suspected that cows from abroad might play main role of transmission of Q fever in Indonesia. However, there was also report of positive result in local ruminants, such as Bali cattle, goat, and sheep (Mahatmi et al. 2007). Researches in various country also stated of potential local ruminant as reservoir animal,  among them are Tibet sheep in China, Alpine and Saanen goat in Italia, camel (Camelus dromedaries) in Saudi Arabia, or Swedish dairy cattle in Swedia (Mohammed et al. 2014). Based on the historical study of Q fever in Indonesia, this research was performed by taken sample from both ex-import cattle and local ruminants in several region in East Java (Malang, Surabaya, Madura), Central Java (Boyolali), and West Java (Bogor, Bandung, Depok) which are known as regions with high population of ruminants in Indonesia.  So far, surveillance data of Q fever has not been recorded from these regions.
PAT-6 Brain Histopathology of Cynomolgus Monkey (Macacafascicularis) with Memory Impairment Indicated by Alzheimer Type Neurodegenerative Disease Vinka Aftinata Kusumaputri; Huda Shalahudin Darusman; Dewi Ratih Agungpriyono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

The study is about the degenerative lesion found in nine cynomolgus monkeys that showed decline cognitive function during theirold age life. In previous studies, the brain of these cynomolgus monkeys has been tested positive for amyloid deposits by using the ELISA test.
PAT-7 A Natural Case Like Diabetes Rat as A New Approach on Understanding Alert of Glucose Methabolism Adi Winarto; Komariah Komariah; Kiranadi Bambang; Manalu Wasmen; Ekowati Handharyani
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Currently, diabetic case as a degenerative disease has widely known that it can be cure by insulin.  A remain puzzle is proliferation do not found in rest Beta cells, so insulin injection has to be applied entire life of diabetic patient. So far, there is no diagnostic approach that expresses such of thing which may indicate an early anomaly on glucose metabolism process. The aim of this research to get case model of hyperglycemic rat that has long period in process.
PCS-1 Development of Mouse Parthenogenetic Embryos in Phosphate Free Medium Vista Budiariati; Dwi Budiono; Mokhamad Fahrudin; Berry Juliandi; Ratih Rinendyaputri; Arief Boediono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Parthenogenesis is an artificial oocytes activation process without paternal contribution so that embryos will develop without fertilization [3]. The process of parthenogenesis as a reproductive strategy occurs in species of insect, pisces, or amphibian, which not require any implantation. Naturally, parthenogenesis is not common in mammals, but by understanding cellular mechanism during fertilization, it is possible to artificially activate mammalian oocytes.Blastocyst, derived from parthenogenesis, can be used for developmental study, embryo reconstruction, and one of potential source for pluripotent stem cells. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate.One of the component that has been predicted to inhibit parthenogenetic embryo development is phosphate. Haraguchi et al. (1996)    reported that phosphate caused a negative effect on in vitro culture of AKR/N mice fertilized embryos, removal of phosphate elements was significantly improved the blastocyst rate up to 42.6% [1]. The effects of phosphate also became an interesting finding in the study that reported mouse fertilized embryos could well developed in modified medium rat 1 cell embryo medium (MR1ECM) which not contained any phosphate [2].The effect of phosphate on in vitro culture of mouse parthenogenetic embryo has not been clear. The aim of this research was to analyze inhibitory effect caused by phosphate in the medium and compare the development pattern between parthenogenetic and fertilized embryos in order to reach optimal production of parthenogenetic blastocyst for further purposes.  
PCS-2 Specialized Structured Cardiovascular of Bats Related to Their Ability to Fly Anisa Rahma; Desrayni Hanadhita; Hera Maheshwari; Aryani Sismin Satyaningtijas; Srihadi Agungpriyono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Bats have the second largest diversity in mammalian classes after rodentia (Nurfitrianto et al., 2013). Bat population reaches approximately 1100 species (Teeling et.al 2005). In Indonesia there are 205 or 21% of known world bat species (Suyanto 2001). Bats are the only flying mammals. The ability bat to fly is supported by the shape of the extremities and also the adaptation of the cardiovascular organs. Based on empirical studies of cardiovascular was known that cardiac muscle mass is a good indicator for the degree of adaptive specialization of prolonged locomotor activity, in both birds and mammals. Data on cardiovascular muscle morphology and muscles that support flying ability will provide an overview of the behavior, ecology and physiology of certain animals (Bishop 1997).Several studies have been conducted to study the cardiovascular system of bats. Research on bat hematology profile has been done (Heard and Whittier 1997, Mclaughlin et.al 2007, Wawrocka and Bartonicka 2014, McMichael et al. 2015, Ratnasooriya 2016, Rashid et al, 2016, Rahma et al., 2018). Joseph (1908) says that every animal has a different size and heart shape to each other. This heart size difference is influenced by the size of the body and the high energy required by the animal (Joseph 1908).Bat's heart rate is known to increase during flight (Thomas and Suthers 1972). This is causes an increase in the rate of metabolism. Writing this paper will discuss about the uniqueness of the bat's heart so that it can support its ability to fly.
PCS-3 Toxicity Sub-cronic Ethanolic Extract of Malaka (Phyllanthus emblica) Leaves on Kidney Function of Mice (Mus musculus) be Reviewed from Blood Creatinin Level N Asmilia; D Aliza; T Armansyah TR; Rusli Rusli; N Tridayanti
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Herbal medicine is still the mainstay of about 75-80% of the whole population, and the major part of traditional therapy involves the use of plant extract and their active constituents in the indigenous. The plant genus Phyllantus is widely distributed in most of tropical and subtropical countries like China, India, Southeast Asia and Indonesia [1,2]. Phyllantus spesies have long been used in folk medicine to tread a broad spectrum of disorders. Phyllantus emblica or Indian gooseberry (Figure 1), belonging to family Euphorbeaceae has been reported to have antioxidant, anti-inflammatory, anticancer, adaptogenic, anti-diabetic, antimicrobial and immunomodulatory potential [3]. The aim of the study was to investigate the effect of ethanolic extract of malaka leaves (Phyllanthus emblica) on renal function by analyzing creatinine level in mice blood (Mus musculus).
PCS-4 In Vivo Embryo Production at Cipelang Livestock Embryo Centre Faisal Amri Satrio; Ni Wayan Kurniani Karja; M Imron; Yuni Siswani; Oloan Parlindungan; Bambang Purwantara
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Livestock Embryo Center/Balai Embrio Ternak (BET) Cipelang is one of the government institution under the supervision of Directorate General of Livestock and Animal Health Services, Ministry of Agriculture of the Republic of Indonesia. BET Cipelang has the main task of carrying out the production, development, and distribution of livestock embryo for all regions of Indonesia. BET Cipelang produced embryos using two methods, there are in vivo and in vitro. The produced embryos will be transferred to the recipient cow using embryo transfer technique.                Embryo transfer biotechnology can be used to enhance genetic improvement and to increase marketing opportunities with purebred cattle. Because of their relatively low reproductive rate and long generation interval, embryo transfer is especially useful with cattle. The success of embryo transfer depends on factors associated with the embryo, the recipient or an interaction among the factors of the embryo and recipient [1].                 Embryo quality is one of the main factors for successful embryo production. In vivo embryo production has better quality than in vitro such as cell number, morphology, growth and development ability and after freezing.  The frequency of occurrence of chromosomal abnormalities in in vivo-derived embryos are lower than in vitro and the consequence is that only 30-40% of oocytes resulting from in vitro maturation develop into blastocysts after in vitro fertilization and the in vitro yield rate of embryos are lower than from in vivo [2]. Recently, there are no studies about the performance of in vivo embryo production at BET Cipelang. The aim of this study is to explore the quality of in vivo embryos produced by BET Cipelang.
PCS-5 Nitric Oxide Induced Basal Cell Hyperplasia and Lamina Propria Elongation in Rat Gastroesophageal Junction Tena Djuartina; Bambang Pontjo Priosoeryanto; Ari Fahrial Syam; Ahmad Aulia; Tri Isyani Tungga Dewi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

NO (Nitric Oxide) is an inorganic compound composed of nitrogen and oxygen, NO is also produced in various places on various types of mammalian cells. NO as a radical compound is important in mediating physiological and pathological events in mammals including humans [1].GEJ (gastroesophageal junction) is a transition zone between the surface of esophagus which is covered by stratified squamous epithelium to the gastric mucosa which consists of simple columnar epithelium (z-line), where circular muscle of esophagus fuse with oblique muscle and lipid layer of the gaster. At the lower part of GEJ, there is the lower esophageal sphincter (LES) that not only allow food to move into stomach and works as an exit passage of the gas, but also inhibit reflux of any substances that potentially can cause harm to the esophagus [2].Petersson et al, found that chronic exposure to cytotoxic levels of NO can cause inflammation, intestinal metaplasia and neoplasia. Although it is known that gastric acid, pepsin and bile acids can cause adenocarcinoma of distal esophagus and GEJ, NO exposure and nitrosative stress role in this phenomenon is yet to be fully understood and further study is needed [3].The purpose of this was to identify and compare the histopathological changes occurring in GEJ in relation to administration of physiological concentration of nitrate dissolved in HCl and ascorbic acid. As such, the animal model used in this study can be used to study and represent the changes microscopically, because obtaining a full thickness biopsy from a human subject can be difficult to perform.