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Indonesian Journal of Life Sciences

IJLS
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Published by Indonesia International Institute for Life Sciences

ISSN : - EISSN : 26560682 DOI : http://doi.org/10.54250/ijls

Core Subject : Health, Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Medicine & Pharmacology

The Indonesian Journal of Life Sciences (IJLS) is a journal published by Indonesia International Institute for Life Sciences Press (i3L Press). IJLS is a scientific journal contains original research articles, review articles, and short communication covering all aspects in life sciences. This journal provides immediate open access to its content on the principle that making research freely available to the public to support a greater global exchange of knowledge. Readers can view abstracts and download full-text articles (PDF) free at any cost. All manuscripts submitted to the Indonesian Journal of Life Sciences will be screened for plagiarism and blind peer-reviewed to present valuable and authentic findings in life sciences. Authors with original, clearly analyzed, and logically interpreted research result in the scope of food innovation and nutrition, bio-products and services for sustainable society, information technology in life sciences, as well as health and well-being are very welcome to submit their articles. Every article issued to the editorial of the Indonesian Journal of Life Sciences is free of charge including the cost of repairing the article. IJLS is published bianually on March and September. Manuscripts are accepted on a continuous basis and the submisison is opened through the year.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 71 Documents

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Bioprocessing of Avian Influenza VLP Vaccine using Baculovirus-Insect Cell Expression System Chrisdianto, Matthew; Damai, Fedric Intan; Mulyono, Roselyn; Virginia, Jesslyn Audrey; K, Katherine
Indonesian Journal of Life Sciences 2022: IJLS Vol 04 No .01
Publisher : Indonesia International Institute for Life Sciences

Vaccines are widely used as a preventive measure against influenza virus infection. However, these vaccines gain concerns regarding their biosafety due to implementing the highly pathogenic avian influenza in the production process. A breakthrough that uses insect cells due to their ability to produce protein rapidly, especially viral antigens for the potential avian influenza outbreak, is being extensively researched. Insect cells infected by baculovirus (BV) are utilized to express proteins known as virus-like protein (VLP). The objective of this review is to assess the production of the avian influenza vaccine (i.e., H5N1 and H7N9 strains) made from VLP by utilizing a baculovirus-insect cell (BV-IC) expression system. A narrative review was conducted by screening international indexed journals from the last 10 years about the topic. The result shows that VLP vaccine development using BV-IC expression can be a cheaper and safer alternative to conventional vaccines while also producing a high yield. The upstream process consists of the IC infection by the BV and BV-IC cell cultivation inside the bioreactor. The downstream process consists of the purification of the VLP product until it becomes a functioning vaccine. The VLP vaccine has improved immunogenic quality, enabling a more specific immune response than other vaccines. However, studies performed on avian influenza vaccines produced by the BV-IC expression system are still lacking. Therefore, further studies are required to improve the current VLP vaccine production processes.

Processing Condition and Properties of Protein Isolates from Mung Bean (Vigna radiata) and Quinoa (Chenopodium quinoa Willd) Dewi, Desak Putu Ariska Pradnya
Indonesian Journal of Life Sciences 2022: IJLS Vol 04 No .01
Publisher : Indonesia International Institute for Life Sciences

Mung bean and quinoa are good sources of protein from legumes and pseudo cereals respectively. Both sources have relatively high protein content and acceptable amino acids composition. The potential and application of mung bean and quinoa as protein isolates have been discovered in some studies. Several isolation techniques such as wet fractionation (acid-base extraction), micellization, dry fractionation, aqueous separation or combination of some techniques can be used to isolate protein. This review summarized different methods and processing conditions applied during production of mung bean and quinoa protein isolates. Extracting protein from different sources may require different conditions, which results in different compositions and functional properties. Acid-base extraction is the most common method applied in mung bean and quinoa and results in high protein purity and yields. Micellization is an alternative method used in mung bean to produce higher protein content. Dry fractionation is a sustainable option used in quinoa to concentrate protein fractions. Purification methods such as ultrafiltration and aqueous phase separation can be used. Different methods and processing conditions affect functional properties, including solubility, water and oil absorption, emulsification, foaming, thermal properties of isolates, which also affect the suitable application for isolates. Keywords: Protein isolates; Quinoa; Mung bean; Wet fractionation; Dry fractionation

Anticancer Activity of Typhonium flagelliforme: A Systematic Review Crystalia, Audrey Amira; Hillary, Hillary
Indonesian Journal of Life Sciences 2022: IJLS Vol 04 No .01
Publisher : Indonesia International Institute for Life Sciences

Cancer is a global health issues that can affect anyone. Cancer is treated conventionally with surgery, chemotherapy, radiotherapy, and hormone therapy. However, the high cost of conventional treatments is a burden for cancer patients. Therefore, many cancer patients seek for cheaper yet effective alternative treatments. Typhonium flagelliforme is a taro-like plant that can be found across Indonesia. Numerous researches on the anticancer effect of T. flagelliformehave been conducted. However, a systematic review on the anticancer property of T. flagelliforme is still lacking. Therefore, this review aimed to systematically evaluate the scientific evidence for the anticancer activities in T. flagelliforme. Five databases were used as the search engine using the designated search terms and studies were selected based on the inclusion criteria. The anticancer evaluation in 30 studies selected were conducted in leukemia, lymphoma, breast, oral, cervical, lung, liver, colon, and squamous cell carcinoma. The result showed that T. flagelliforme could inhibit cancer cell proliferation with most of the IC50 are less than 200 μg/mL. T. flagelliformeinduced an increase of caspase-3 and -9, as well as a decrease of the anti-apoptotic Bcl-2 protein expression. The expression of p21 protein was increased after treatment of T. flagelliforme extract while the tyrosine kinase, Ki67, HER2/neu, telomerase, and COX-2 expressions were decreased implying T. flagelliforme could inhibit tumor growth and development. Lastly, T. flagelliforme is also capable in reducing the possibility of cancer cell invasion. Findings suggest that T. flagelliforme is potential to further developed for cancer treatment.

The Effects of Simvastatin and Soursop (Annona muricata) Leaf Extract on Colorectal Cancer Amadea, Ignatia Ceraphine; Atrasina, Dina
Indonesian Journal of Life Sciences 2021: IJLS Vol 03 No .01
Publisher : Indonesia International Institute for Life Sciences

Colorectal cancer (CRC) is a heterogeneous disease of the colon and rectum. In Indonesia, CRC has the fourth highest cancer incidence, with about 30,000 new cases and causing around 16,000 deaths per year. Numerous studies have shown the benefits of using simvastatin and Annona muricata (A. muricata) leaf extract to promote cytotoxic effects in CRC cells. This systematic review aimed to evaluate the effects and the possible molecular mechanisms of actions used by simvastatin and A. muricata leaf extract to exert anti-cancer effects in CRC cells. We synthesized published studies on the effect of simvastatin and A. muricata leaf extract in in vitro and in vivo CRC studies, and highlighted the potential application of simvastatin and A. muricata leaf extract in combination with other anti-cancer treatments. This systematic review was written in accordance with the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA). Simvastatin and A. muricata leaf extract were shown to suppress CRC cell growth and proliferation and generate a synergistic effect when combined with other anti-cancer treatments. Several possible mechanisms of actions of simvastatin include bone morphogenetic protein (BMP) pathway and mevalonate pathway activation, ERKs inactivation, and regulation of the yes-associated protein 1 (YAP1), while for A. muricata leaf extract, possible mechanisms of actions include intrinsic apoptosis pathway activation, cytochrome c-mediated apoptosis, mitochondria-mediated apoptosis, and programmed cell death initiation. Further experimental evidence is needed to determine the specific mechanisms on how simvastatin and A. muricata leaf extract induce anti-cancer effects against CRC cells.

Correlation Between miR-223 Expression and Rheumatoid Arthritis A, Angelika; Djohan, Carren Kwoknata; Julianto, Nadya Marcelina; Putri, Tiffanny Adora; Julietta, Vivi; Ratnasari, Nanda Risqia Pradana
Indonesian Journal of Life Sciences 2021: IJLS Vol 03 No .01
Publisher : Indonesia International Institute for Life Sciences

Patients diagnosed with rheumatoid arthritis (RA) show symptoms of joint affiliations. Focusing on the epigenetic factors leading to RA, the expression of several miRNAs are assumed to be one of the components influencing the cause of RA. Consequently, the increased expression of miR-223 is hypothesized to be involved in the pathogenesis of RA. This study aims to analyze the increased expression of miR-223 of RA patients in comparison with the normal and healthy representatives. Five methods from five different studies were involved in this review. The RNA was first isolated from heparinized venous blood or peripheral blood of RA patients and healthy controls. Reverse transcription was done to convert isolated RNA into cDNA and the expression of miR-223 was then measured using real-time PCR. The expression of miR-223 had exhibited a considerable increase in its concentration on RA patients when compared to healthy controls. Besides, the heightened concentration is taken upon consideration as miR-223 has a role in the regulation of the immune system and inflammatory responses.

Recent Advancements of Fungal Xylanase Upstream Production and Downstream Processing J, Jonathan; Tania, Veren; Tanjaya, Jessica C.; K, Katherine
Indonesian Journal of Life Sciences 2021: IJLS Vol 03 No .01
Publisher : Indonesia International Institute for Life Sciences

Xylanase is a hydrolytic enzyme produced by fungi and bacteria utilized in various industrial applications such as food, biobleaching, animal feed, and pharmaceuticals. Due to its wide variety of applications, xylanase's large-scale industrial production has gained researchers' interest. Many factors and methods affect fungal xylanase's production in both upstream and downstream processing stages. The upstream production methods used are submerged fermentation (SmF) and solid-state fermentation (SSF), where SmF involves the usage of liquid substrates, while the SSF applies solid substrates to inoculate the microbes. The downstream processing of fungal xylanase includes extraction, purification, and formulation. The extraction methods used to extract fungal xylanase are filtration and solvent extraction. Meanwhile, the purification methods include ultrafiltration, precipitation, chromatography, Aqueous Two-Phase System (ATPS), and Aqueous Two-Phase Affinity Partitioning (ATPAP). The formulation of xylanase product is obtained in either liquid from the extraction-purification results, which can be converted to powder form using technologies such as spray drying to increase storage life. Moreover, immobilization of xylanase with nanoparticles of SiO2 could produce reusable xylanase enzymes. Several future studies have also been suggested. This review aims to explain the upstream and downstream processes of fungal xylanase production as well as the factors that affect those processes.

Implementation of Biodesign process to Identify Unmet Needs of Tuberculosis Patients in Indonesia Siswanto, Melinda; Thimoty, Gabriella; Bera, Jonathan Hendrik
Indonesian Journal of Life Sciences 2021: IJLS Vol 03 No .01
Publisher : Indonesia International Institute for Life Sciences

Tuberculosis (TB) is an infectious disease mainly caused by Mycobacteria tuberculosis. It affects the respiratory system and may spread to the other organ. Due to the nature of the bacteria, infection of this species may cause a long-term TB, causing it a challenge for a full clearance. The disease has existed throughout the history, and BCG vaccine has been developed to control the spreading. Most developed countries have successfully eradicated TB. However, Indonesia is currently ranked second place for this disease, making it as a pressing matter to eradicate. This study is conducted to investigate and gather deep insights about the problems in eradicating TB in Indonesia. Identify part of the biodesign method is employed in the study to elucidate the complexity of healthcare issue of TB in Indonesia. The study discovered that there are three pillars’ sectors need to be addressed: education, healthcare facilities and services, and government. For successful eradication, synergistic approach to all pillars at the same time are essential. The needs statements formulated in this study could be the stem of innovation in TB.

A Review on Bacterial Cellulose: Properties, Applications, Fermentative Production, and Molasses Potential as Alternative Medium Angela, Clara; Devanthi, Putu Virgina Partha
Indonesian Journal of Life Sciences 2021: IJLS Vol 03 No .01
Publisher : Indonesia International Institute for Life Sciences

Bacterial cellulose (BC) is an extracellular homopolymer produced by certain species of bacteria. It has demonstrated the potential as an alternative to plant cellulose with more appealing features such as unique nanostructure, high porosity, high crystallization index, high tensile strength, high water holding capacity, and a high degree of polymerization. These attributes facilitate BC utilization in various applications, ranging from the food industry, cosmetics, pharmaceutical, medical field, waste treatment, textile, and paper industry. Considering the advantages and wide range of applications, it is necessary to explore and improve the current industrial production to achieve a higher yield at a lower cost. This review article summarizes the BC properties and characteristics as well as its application in different fields. Furthermore, the potential of agricultural waste molasses for low-cost BC production is also discussed.

Phytochemical Screening and Therapeutic Effects of Binahong (Anredera cordifolia (Ten.) Steenis) Leaves Salim, Angeline; Kristanto, Dandy Felix; Subianto, Felicia; Sundah, Janice Evita; Jamaica, Putri Avanny; Angelika, Tiara; Maulida, Nurul Fajry
Indonesian Journal of Life Sciences 2021: IJLS Vol 03 No .02
Publisher : Indonesia International Institute for Life Sciences

In this review, the function of secondary metabolites in Binahong (Anredera cordifolia (Ten.) Steenis) leaves were summarized for their therapeutic effects such as antimicrobial activity, anti-inflammatory, wound healing, lowering the blood pressure, and antihyperlipidemic activity as a potential medicinal plant to treat disease. The phytochemical screening of Binahong leaves extracted by using particular solvent showed positive results to the presence of phenolic, steroid, terpenoid, alkaloid, and saponin compounds contained in the extract. The antimicrobial activity is able to disrupt cell membrane activity and inhibit bacterial growth, meanwhile anti-inflammatory ability is able to decrease anti-inflammatory agents level. It also decreases blood pressure by regulation of lipid metabolites, reduction of peripheral resistance, and upregulation of nitric oxide activity. The ethanolic extract of Binahong leaves can reduce fat deposits or layers in the endothelial cells, cholesterol, triglycerides, and low-density lipoprotein (LDL) cholesterol levels, and decrease the levels of malondialdehyde (MDA formation) from the lipid peroxidation to prevent hyperlipidemia and heart disease.

The Stability of Phycocyanin, Phycoerythrin, and Astaxanthin from Algae Towards Temperature, pH, Light, and Oxygen as a Commercial Natural Food Colorant Goyudianto, Bryan Ashley; Meliana, Catarina; Muliani, Debby; J, Jeslin; Sadeli, Yohana Elma; Ratnasari, Nanda Rizqia Pradana
Indonesian Journal of Life Sciences 2021: IJLS Vol 03 No .02
Publisher : Indonesia International Institute for Life Sciences

Nowadays, food industries are exploring more about naturally-derived colorants. Algae is proposed to be an excellent alternative source for natural colorants as it needs lesser biomass. Phycocyanin, phycoerythrin, and astaxanthin are commercially used blue-green, red, and red-orange algae-sourced pigments due to their high protein yield, health benefits, and ease of extractions methods. A literature survey conducted using Google Scholar and ScienceDirect database with inclusion and exclusion criteria gained 44 papers used as primary references to assess those algae pigments' stability towards temperature, pH, light, and oxygen for food applications. Low pH levels and addition of preservatives (sugar, citric acid) or polyhydric alcohols enhance phycocyanin range of stability (pH of 5–6 and >40oC with pH >5 or <3). Phycoerythrin’s stability at -20 to 4°C and neutral pH is improved by adding additives (citric acid, benzoic acid) or nanofibers, cross-linking method, complex formation, and microencapsulation. Phycocyanin and phycoerythrin’s light stability depend on the light’s composition, quality, and quantity; hence, utilization of dark-colored packaging to prevent light exposure is done. Astaxanthin’s instability towards light exposure (causing photoexcitation), temperature of >30°C, and pH of >4 can be solved through chitosan solution coating and microencapsulation using various wall materials and complex formation. Phycocyanin is unaffected against oxygen (unlike phycoerythrin and astaxanthin), yet all of them exert antioxidant properties. Therefore, the inconsistency of these colorants’ stability depending on food processing conditions demand further development through research to widen their commercial food applications.

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Slamet Supriadi

Contact Email slamet.supriadi@i3l.ac.id

Phone +622129567888

Journal Mail Official ijls@i3l.ac.id

Editorial Address Indonesian Journal of Life Sciences LPPM - Indonesia International Institute for Life Sciences (i3L) Jl. Pulomas Barat Kav. 88, Jakarta Timur, 13210 Indonesia

Location Kota adm. jakarta timur, Dki jakarta INDONESIA

Abstract

Abstract

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Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Slamet Supriadi

Contact Email
slamet.supriadi@i3l.ac.id

Phone
+622129567888

Journal Mail Official
ijls@i3l.ac.id

Editorial Address
Indonesian Journal of Life Sciences LPPM - Indonesia International Institute for Life Sciences (i3L) Jl. Pulomas Barat Kav. 88, Jakarta Timur, 13210 Indonesia

Location
Kota adm. jakarta timur,

Dki jakarta

INDONESIA