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Ivandini Tribidasari A.
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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
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Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 20 Documents
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Search results for , issue "Vol. 13, No. 2" : 20 Documents clear
EKSPRESI RELATIF mRNA HIF-1α PADA JANTUNG, OTAK DAN DARAH TIKUS SELAMA INDUKSI HIPOKSIA SISTEMIK Wanandi, Septelia Inawati; Dewi, Syarifah; Paramita, Reni
Makara Journal of Science Vol. 13, No. 2
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Relative Expression of HIF-1α mRNA in Rat Heart, Brain and Blood During Induced Systemic Hypoxia. Hypoxia is a pathological condition in which the body as a whole or region of the body (tissue or cell) deprived of adequate oxygen supply. The transcriptional regulator hypoxia inducible factor-1 (HIF-1) is an essential mediator of O2 homeostasis. Unlike the β sub unit (HIF-1β), the activity of HIF-1α is controlled in an oxygen-dependent manner. It has been reported that the stability and expression of HIF-1α during hypoxia is remarkably higher than those under normoxic conditions.The aim of this study was to analyze the adaptive tissue responses during induced systemic hypoxia by comparation of relative expression of mRNA HIF-1α in rat heart, brain and blood. Twenty-five male Sprague Dawley rats were subjected to systemic hypoxia by placing them in the hypoxic chamber supplied by 8-10% of O2 for 0, 1, 7, 14 and 21 days, respectively. The relative expression level of HIF-1α mRNA in brain, heart and leucocyte cells were analyzed using quantitative RT-PCR assay (Real Time PCR) based on Pfaff’s formula. This study demonstrates that the increased of relative expression of HIF-1α mRNA during induced systemic hypoxia reached its maximum level at day 7 (in heart) or at day 14 (in brain), whereas in leucocyte cells the stimulation of HIF-1α expression was intensively maintained up to 21 days although the expression has reached the remarkably high level. We could conclude that HIF-1α as an oxygen sensing during systemic hypoxia has different capacity and sensitivity in brain, heart and blood tissues, due to the importance of oxygen homeostasis in each tissue
PENGARUH PENYUNTIKAN DOSIS MINIMAL DEPOT MEDROKSIPROGESTERON ASETAT (DMPA) TERHADAP BERAT BADAN DAN KIMIA DARAH TIKUS JANTAN GALUR SPRAGUE-DAWLEY Yurnadi, Yurnadi; Suryandari, Dwi Anita; Moeloek, Nukman
Makara Journal of Science Vol. 13, No. 2
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Effect of Injection Minimal Dosages of Depot Medroxyprogesterone acetate (DMPA) to Body Weight and Blood Chemistry Male Rat Strain Sprague-Dawley. Many family planning program focus more on men. Until now, vasectomy has been the commonly used method for male contraception. However, this method creates inconvenience such as irreversibility and psychological problems. One of the alternatives contraception is the combination of depot medroxyprogesterone acetate (DMPA) and androgen. The minimum dosage of DMPA could suppress testosterone level that leads to reduced spermatogenesis and sperm viability. Nevertheless, until now it is not known whether minimum dosages of DMPA have an effect to body weight and blood chemistry. Therefore, this research aimed at determinate the effect of minimal dosages of DMPA to body mass and blood chemistry using male rats (Rattus norvegicus L.) strain Sprague-Dawley as model. This research using completely randomized design, unequal size sample, castration treatments and several doses DMPA (1.25, 0.625, and 0.313 milligram). Injecting of DMPA conducted intramuscularly on week 0 and week 12. Normality/homogeneity Data normality were analyzed before ANOVA test. Then, abnormal data were tested using Kruskal-Wallis test. The result shows that injection of DMPA in various doses do not have an effect on body weight and blood chemistry such as erytrocytes, haemoglobin, hematocrite, HDL, LDL, total cholesterol, SGOT, SGPT and triglyseride) (p>0,05). Furthermore, it is concluded that that no effect of minimal dosages of DMPA to body mass and blood chemistry of rat.
PEMBERIAN AKAR PASAK BUMI (Eurycoma longifolia Jack.) PADA INDUK LAKTASI UNTUK MENINGKATKAN BOBOT BADAN ANAK MENCIT Panjaitan, Ruqiah Ganda Putri; Jayuska, Afghani; Harahap, Zulfan; Zakiah, Zulfa
Makara Journal of Science Vol. 13, No. 2
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The Effect of Administration of Pasak Bumi (Eurycoma longifolia Jack.) Roots on Lactating Mice to Increase Body Weight Pups. The roots of Eurycoma longifolia Jack have been used as a traditional medicine for aprodisiac. The effects of the root extract of E. longifolia Jack were studied on lactating mice. Each mice was administered metanol extract and its derived fractions at dose 500 mg/kg rat body weight for 21 consecutive days in lactation period. Control positive group received Moloco+B12 at dose 0,13 g/kg rat body weight, negative control (placebo) group received 2 ml/kg rat body weight of aquadest daily and normal control group. No differences between normal control, negative control, positive control, and metanol extract and its derived fractions. The administration methanol-water fraction to lactating mice increasing body weight mice pups similar to Moloco+B12.
CuInSe2 THIN FILM FOR SOLAR CELL BY FLASH EVAPORATION Soepardjo, A H
Makara Journal of Science Vol. 13, No. 2
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Deposition of thin films for material solar cell CuInSe2 are relatively simple. In this research mainly focused on the use of flash evaporation method, and the material created can then be characterized by optical and electrical properties. The optical characterization is done by X-ray Diffraction (XRD), Energy Dispersive Spectroscopy (EDS), and transmission and reflection by UV-VIS spectrophotometry. Electrical characterization is done by utilizing the Hall effect equipment. From these characterization, the atomic structure, absorption coefficient, energy gap, material type, composition of each elements and the mobility of CuInSe2 can be measured and determined. During process evaporation were carried out at substrate temperatures the range between 20ºC–415ºC.
CRYSTAL STRUCTURE OF [Bis(DIMETHYLFORMAMIDE) Bis(2,2’-PYRIDYL)QUINOLINE)IRON(II)]Bis-(TETRAPHENYLBORATE) Jahro, Iis Siti; Onggo, Djulia; Ismunandar, Ismunandar; Rahayu, Susanto Imam; Yamin, Bohari M.
Makara Journal of Science Vol. 13, No. 2
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The goal of this research is to obtain single crystal and structural information of iron(II) complex with 2,(2’- pyridyl)quinoline(pq) ligands. The reaction of iron(II) salt with 2,(2’-pyridyl)quinoline ligand and sodium tetraphenylborate in the molar ratio of 1:3:2 in methanol-N,N-dimethylformamide(dmf) solution results in an iron(II) complex. The formula of the [Fe(pq)2(dmf)2](BPh4)2 complex has been obtained from the iron(II) and C, H, N contents. Single crystal of [Fe(pq)2(dmf)2](BPh4)2 suitable for X-ray investigation was obtained by evaporation of the complex solution in N,N-dimethylformamide at room temperature after 24 hours. This compound crystallizes in monoclinic system with C2/c space group, a = 27.950(4), b = 14.169(7), c = 17.717(9) Å and β = 105.669(11)°. The structure consist of iron(II) is chelated by two pq ligands through the N atoms and two dmf molecules in a six-coordination environment. The charge of the [Fe(pq)2(dmf)2] 2+ cation is balanced by two tetraphenylborate (BPh4) - anions.
BIODIESEL FROM LOW GRADE USED FRYING OIL USING ESTERIFICATION TRANSESTERIFICATION PROCESS Hasibuan, Sawarni; Ma’ruf, Amar; Sahirman, Sahirman
Makara Journal of Science Vol. 13, No. 2
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Biodiesel from Low Grade Used Frying Oil Using Esterification Transesterification Process. The aim of this research was to obtain the optimal condition for the production of biodiesel from low grade used frying oil through the esterification transesterification process. The experiment was arranged in Completely Randomized Design with Duncan’s advanced test. Results showed that the biodiesel FFA content is ranged between 0.167 to 0.795% (P
PRODUKSI GAS HASIL BIODEGRADASI MINYAK BUMI: KAJIAN AWAL APLIKASINYA DALAM MICROBIAL ENHANCED OIL RECOVERY (MEOR) Astri, Astri
Makara Journal of Science Vol. 13, No. 2
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Gas Production Generated from Crude Oil Biodegradation: Preliminary Study on its Aplication in Microbial Enhanced Oil Recovery (MEOR). The objective of this study is to observe the capacity of gas production generated from crude oil degradation by the isolated bacteria. The gas in the MEOR could increase pressure in the reservoir, decrease oil viscosity, increase oil permeability-due to the increase of the porosity and viscosity, and also increase oil volume due to the amount of dissolved gas. A research on gas analysis of oil degradation by 6 isolated bacteria has been conducted. The bacteria isolates including Bacillus badius (A), Bacillus circulans (B), Bacillus coagulans (C), Bacillus firmus (D), Pasteurella avium (E) and Streptobacillus moniliformis (F). The trial on gas production, gas analysis and oil degradation analysis, was carried out by using SMSS medium. The test of gas production was done by using microrespirometer at 40°C. The result shows that B, C, D, E produce more gas than A and F. Gas of CO2, O2, CO, N2, CH4, and H2 were analyzed by using GC. The results show that only three gases were detected by GC i.e. CO2, N2, and O2. The concentration of CO2 and N2 gas increased while the concentration of O2 decreased over an 8th day of observation. CO2 gas producted by mix culture was higher than by the pure culture. On the 8th day of incubation, the production of CO2 gas by mix culture was 4,0452% while pure culture C and D only produced 2,4543% and 2,8729%. The mix culture increase simple hydrocarbon by 12.03% and the formation of a complex hydrocarbon by 3.07%. The mix culture (C-D) generated the highest concentration of CO2 gas as well as a synergistic concortium that has ability to degrade crude oil.
TINGKAT PENCEMARAN LOGAM BERAT DALAM AIR LAUT DAN SEDIMEN DI PERAIRAN PULAU MUNA, KABAENA, DAN BUTON SULAWESI TENGGARA Ahmad, Fasmi
Makara Journal of Science Vol. 13, No. 2
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The Degree of Heavy Metals Pollution in Seawater and Sediment in the Waters of Muna, Kabaena and Buton Island, Norteast of Sulawesi. Muna, Kabaena, and Buton are islands found in Northeast of Sulawesi. One of them, namely Kabaena Island is island which has high Nickel content. Some of mining industries has explored the Nickel content in this area. Tailing come from mining thrown to the sea, will increase the heavy metals content in seawater and sediment in the waters of that third island, remembering the position of that third island not so far. In order to marine pollution monitoring by heavy metals, Research Center for Oceanography has done some marine research in the waters of that third island in April 2006. The aim of this research is to know the impact of Nickel mining activities to heavy metals content in seawater and sediment in that third waters. Heavy metals monitored is Pb, Cd, Cu, Zn, and Ni. The results showed that the content of heavy metals Pb, Cd, Cu, Zn, and Ni in sea water still in line with the threshold value according to Kep MNLH No. 51, 2004 for sea biotas. The same condition also found in sediment, where the content of Pb, Cd, Cu, Zn, and Ni is low relative, and still in line with heavy metals content found in normal seawater.
OPTIMASI BIODEGRADABILITAS DAN UJI TOKSISITAS HASIL DEGRADASI SURFAKTAN LINEAR ALKILBENZENA SULFONAT (LAS) SEBAGAI BAHAN DETERJEN PEMBERSIH Budiawan, Budiawan; Fatisa, Yuni; Khairani, Neera
Makara Journal of Science Vol. 13, No. 2
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Optimization of Biodegradability and Toxicity Testing of Degradation Product from Linear Alkyl Benzene Sulfonate (LAS) Surfactant as Cleaning Detergent Agent. Linear Alkyl Benzene Sulfonate (LAS) is a surfactant used in laundry detergent as cleaning agent and toxic to aquatic organisms. Results shows, with the LAS concentration used (20 ppm) in medium, adaptation time and Acinetobacter sp. growth has shown better biodegradation ability than three other bacteria used Pseudomonas putida, Pseudomonas fluorescence, Bacillaria spp. Thus, Acinetobacter sp is used further for biodegradation process of LAS. Based on its biodegradation half-life using Acinetobacter sp, and with mixed culture (± 52.32% and ± 46.82% respectively) could be achieved in 4 (four) days, LAS could be categorized as a biodegradable compound. The toxicity assay is based on tetrazolium dye reduction with Rhizobium meliloti as indicator organism. LAS is more toxic than its intermediate product from biodegradation, with IC50 = 34.35 ppm, and the intermediate product, Ac and Cm, has IC50 = 446.19 ppm and 111.28 ppm respectively. Identification of intermediate products using IR and LC-MS analysis shows that the degradation product contains chemicals compounds with functional group as follows: benzene, benzoic acid, hydroxyl, and aliphatic carbons with large molecule weight. Until its half-time degradation time, LAS biodegradation process only occurs at the aliphatic carbon chain, and have not yet reached the stage of aromatic ring opening.
APPLICATION OF HYDROXYAPATITE IN PROTEIN PURIFICATION Yusuf, Permai Sari Molyana; Dahlan, Kiagus; Witarto, Arief Budi
Makara Journal of Science Vol. 13, No. 2
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The precursors, Na2HPO4.2H2O and CaCl2.2H2O are used for synthesizing pure hydroxyapatite which less of carbonate content. The high temperature of sintering, about 700o C of temperature, is treated to minimize the carbonate group on hydroxyapatite surface. Carbonate content of hydroxyapatite which is sintered in 700o C is less than 110o C. It indicates an increasing temperature of sintering will increase crystallinity and decrease carbonate content of hydroxapatite. This method gave better way to resulting hydroxyapatite crystal. The patterns of band of SDS PAGE in resulting of Protein Purification by DEAE matrix is also appear in using hydroxyapatite matrix. The other band also appear in purification by hydroxyapatite matrix, it showed that hydroxyapatite not only has DEAE matrix characteristic as anion exchange but also has kation exchange characteristic. These patterns proof that hydroxyapatite could be used in protein purification as matrix as cation an anion exchange. Additionally, apatite matrix is abiding and can be used repeatedly more than one hundred times without contamination.

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