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THE EFFECTS OF SIMVASTATIN, ROSUVASTATIN, AND FENOFIBRATE ON THE BODY WEIGHT AND LIPID PROFILES OF FEMALE RATS TREATED WITH ORAL CONTRACEPTIVES AND A HIGH-FAT DIET Dwi Anggara Putri; Yulia Yusrini Djabir; Muhammad Akbar Bahar; Gemini Alam; Latifah Rahman; Muhammad Aswad; Muhammad Aryadi Arsyad
Jurnal Kedokteran Hewan Vol 16, No 3 (2022): September
Publisher : Universitas Syiah Kuala

This study aimed to compare the effects of simvastatin, rosuvastatin, and fenofibrate therapies on the body weight and lipid profiles of the female rats receiving a combination of oral contraceptives (COC) and a high-fat diet (HFD). Twenty female Wistar rats (130-200 g) were divided equally into four groups. The rats received a standard diet for seven days, and their baseline lipid profiles were analyzed. All rats also received COC containing 15.1 µg/kg levonorgestrel/3.1 µg/kg estradiol with HFD for 60 days. Starting on day 31, the rats were given the respected treatment. The control group received the vehicle, whereas the others were treated with either simvastatin (2.1 mg/kg), rosuvastatin (0.5 mg/kg), or fenofibrate (8.2 mg/kg). Blood samples were taken on days 30 and 60. The results showed that the administration of COC+HFD and vehicle for 30 days increased the rats’ body weight and dyslipidemia, characterized by a significant decrease in Low-Density Lipoprotein (HDL) and an increase in total cholesterol (TC) and triglycerides (TG) levels (P0.05). Among the treatments, only fenofibrate was found to be able to prevent a significant weight gain in rats. Both fenofibrate and rosuvastatin inhibited a significant rise in TG and TC levels. Meanwhile, the simvastatin administration failed to do the same. Although statistically insignificant, all treatments increased rats’ HDL levels. Thus, it can be concluded fenofibrate was the most effective treatment among all in reducing weight gain and improving the lipid profile of dyslipidemic rats induced by oral contraceptives and a high-fat diet.

THE EFFECTIVENESS OF hCG IN THE DYNAMICS OF FUNCTIONAL STRUCTURE OF SUPEROVULATED ACEH CATTLE OVARIES USING PREGNANT MARE SERUM GONADOTROPINS Mitha Kurnia Sari; Iman Supriatna; Mohamad Agus Setiadi
Jurnal Kedokteran Hewan Vol 16, No 3 (2022): September
Publisher : Universitas Syiah Kuala

This study aimed to determine the biopotency of pregnant mare serum gonadotropin (PMSG) in stimulating the formation of dominant follicles and the biopotency of human chorionic gonadotropin (hCG) in triggering an increase in the ovulation rate of the natural-estrus donor group and the prostaglandin F2α (PGF2α) induced group using female Aceh cattle (n= 20) which were divided into two treatment groups: the group with natural estrus (A) and the group with PGF2α -induced estrus (B). Each donor group was superovulated using the injection of PMSGhCG (A1, B1) and only PMSG (A2, B2). The observations on total follicles and CL were carried out at D10, D14/D+0 and D21/D+7 using ultrasonography (USG). The results showed that PMSG gave a better superovulatory response to the development of dominant follicles (P0.01) at A1, A2, B1, and B2. The administration of hCG hormone 2000 IU i.m. was not able to increase the rate of ovulation. Total follicles after the addition of hCG were also not significantly different between A1:A2 and B1:B2. Statistically, the number of CL in all donor groups in D21 was not significantly different (P0.05). It concluded, superovulation using PMSG gave the same results to the donor cows with natural estrus and the donor cows with PGF2α-induced estrus. The hCG hormone with a dose of 2000 IU injected i.m. was also not able to increase the ovulation rate of Aceh cattle superovulated with PMSG 2500 IU i.m. ____________________________________________________

BODY WEIGHT, MUSCLE WIGHT, PROTEIN, DNA AND RNA CONTENTS IN BREAST MUSCLE (M. Pectoralis Major) OF SELECTED LOCAL MEAT CHICKEN FED ON A DIFFERENT LEVEL OF KIAPU (Pistia stratiotes L.) IN FERMENTED DIET M. Aman Yaman; Sri Jeksi; Muhammad Daud
Jurnal Kedokteran Hewan Vol 16, No 3 (2022): September
Publisher : Universitas Syiah Kuala

The study to evaluate changes in body weight, muscle weight, muscle protein content and total DNA and RNA on breast muscle of selectedlocal chicken due to different levels of fermented feed containing Kiapu (Pistia stratiotes L.) was conducted using a factorial randomized designfor 7 weeks. This study used 72 selected local chickens, which were separated into 2 groups (male and female) and fed on 3 types of feedtreatment, namely P0 (control treatment with 100% commercial feed), P1 (80% commercial feed + 20% fermented Kiapu feed), and P2 (70%commercial feed + 30% fermented Kiapu feed). Parameters were body weight, muscle weight, muscle protein content and total DNA and RNAon breast muscle of chicken. Total protein, DNA and RNA content were analysed by enzymatic reactions of prokaryotic cell and measured with aUV-Spectrophotometer according to the specified OD (optical density). The results showed that feeding Kiapu fermented 20-30% increased bodyweight and protein content of breast muscle of female chicken by change in DNA content. However, the feeding treatment had no changes inmuscle weight and RNA content of both in male and female chicken. On the other hand, substitution of 20-30% Kiapu fermented feed causednegative changes in DNA level of breast muscle. From this study, it was concluded that substitution of fermented feed containing 20-30% Kiapuassociated with the growth process of breast muscle protein is suitable for female but not suitable for male chicken.

EFFECT OF ETHANOL EXTRACT FROM MALACCA LEAVES (Phyllanthus emblica) ON THE SPERM QUALITY OF MICE Cut Nila Thasmi; Nuzul Asmilia; Elfi Satria Suryani; Hafizuddin Hafizuddin; Mulyadi Adam; Arman Sayuti; Nazaruddin Nazaruddin; Budianto Panjaitan
Jurnal Kedokteran Hewan Vol 16, No 3 (2022): September
Publisher : Universitas Syiah Kuala

This study aims to analyze the effect of administering Malacca leaves (Phyllanthus emblica) extract on the sperm quality of mice. This leaf extract was administered orally using gavage method once daily for 21 days. There were four treatment groups: the control group (P0) received distilled water while the first (P1), second (P2), and third (P3) treatment groups received 300 mg, 600 mg, and 1200 mg of Malacca leaf ethanol extract/kg body weight, respectively. Each groups consisted of four mice and was evaluated for sperrm motility, viability, morphology, and concentration. The results showed that sperm motility, viability, morphology and sperm concentration tended to decrease after administration of Malacca leaf ethanol extract for all parameters, although there was no significant difference (P0.05). It was concluded that the administration of Malacca leaf ethanol extract tends to reduce the sperm quality of mice. ___________________________________________________________

THE BIOLOGICAL FUNCTIONS OF IMMUNOGLOBULIN Y (IgY) MOLECULES IN AGAINST INFECTION OF Enterococcus faecalis ORIGIN OF RED TILAPIA Rifky Rizkiantino; Fachriyan Hasmi Pasaribu; Retno Damajanti Soejoedono; Wyanda Arnafia; Dinda Reisinta; I Wayan Teguh Wibawan
Jurnal Kedokteran Hewan Vol 16, No 3 (2022): September
Publisher : Universitas Syiah Kuala

Red tilapia (Oreochromis hybrid) is Indonesia's leading freshwater fishery commodity susceptible to streptococcal bacterial infection. Many studies have been conducted on various efforts to prevent and treat this disease, one of which uses the immunoglobulin Y (IgY) molecule from chicken egg yolk. This study aimed to observe the biological function of IgY against Enterococcus faecalis as a cause of streptococcal-like infection. The agglutinin function was conducted by observing the growth of Enterococcus faecalis in brain heart infusion (BHI) broth media which was added with IgY suspension. The function of inhibin was performed using a spectrophotometric method to measure the level of turbidity of the bacterial suspension inoculated with IgY suspension. The bactericidal potential through the complementary activation pathway for red tilapia serum was carried out using a scanning electron microscope (SEM) method to evaluate the topography of the bacterial cell wall. The results of the study can be concluded that IgY anti-Enterococcus faecalis has the potential as an agglutinin, inhibin, and bactericidal agent through its putative potential in complement activation in streptococcal bacterial infections in red tilapia commodities.

DATE EXTRACT SUPPLEMENTATION IN RINGER LACTATE-EGG YOLK EXTENDER ON POST-THAWING QUALITY OF PELUNG CHICKEN SPERMATOZOA Nu'man - Hidayat; Ismoyowati Ismoyowati; Elly Tugiyanti; Imam Suswoyo; Rosidi Rosidi; Ibnu Hari Sulistyawan; Aras Prasetyo Nugroho
Jurnal Kedokteran Hewan Vol 16, No 3 (2022): September
Publisher : Universitas Syiah Kuala

The research purpose was to evaluate the influence of date extract supplementation in lactate ringer-egg yolk extender with 0.025% sodium dodecyl sulfate (LREYS) on post-thawing quality of Pelung chicken spermatozoa. Completely randomized design with 5 treatments and 4 replications was used in this study. Semen was collected from three Pelung chickens once in three days using the dorsal-abdominal massage method. Semen was divided into 5 treatment groups of date extract supplementation that were 0% as control (LREYSDE0), 0.5% (LREYSDE1), 1% (LREYSDE2), 1.5% (LREYSDE3), and 2% (LREYSDE4). The post thawing liquid semen was observed for sperm motility, viability, plasma membrane integrity and acrosome integrity. The result showed that the motility, viability, membrane plasma integrity and acrosomal integrity of spermatozoa in LREYSE extender with 1.5% date extract supplementation (30.44±1.02%; 49.83±0.91%; 43.26±1.02%; and 45.33±0.72% respectively) was significantly higher (P0.05) than others treatment that were 0% (18.21±0.43%; 35.62±0.51%; 30.54±0.60%; and 31.49±0.71% respectively), 0.5% (20.45±0.72%; 40.72±0.87%; 36.81±0.55%; and 38.56±0.63% respectively), 1% (25.68±0.93%; 46.27±0.75%; 40.52±1.02%; and 42.83±1.09% respectively) and 2% (22.55±0.92%; 43,71±0,74%; 38,84±0,71%; and 41,39±0,86% respectively) of date extract supplementation. It is concluded that the 1.5% date extract supplementation in extender can maintain the best post-thawing sperm quality.

EFFECT OF ETHANOL EXTRACT FROM MALACCA LEAVES (Phyllanthus emblica) ON THE SPERM QUALITY OF MICE Thasmi, Cut Nila; Asmilia, Nuzul; Suryani, Elfi Satria; Hafizuddin, Hafizuddin; Adam, Mulyadi; Sayuti, Arman; Nazaruddin, Nazaruddin; Panjaitan, Budianto
Jurnal Kedokteran Hewan Vol 16, No 3 (2022): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i3.27246

This study aims to analyze the effect of administering Malacca leaves (Phyllanthus emblica) extract on the sperm quality of mice. This leaf extract was administered orally using gavage method once daily for 21 days. There were four treatment groups: the control group (P0) received distilled water while the first (P1), second (P2), and third (P3) treatment groups received 300 mg, 600 mg, and 1200 mg of Malacca leaf ethanol extract/kg body weight, respectively. Each groups consisted of four mice and was evaluated for sperrm motility, viability, morphology, and concentration. The results showed that sperm motility, viability, morphology and sperm concentration tended to decrease after administration of Malacca leaf ethanol extract for all parameters, although there was no significant difference (P0.05). It was concluded that the administration of Malacca leaf ethanol extract tends to reduce the sperm quality of mice. ___________________________________________________________

THE BIOLOGICAL FUNCTIONS OF IMMUNOGLOBULIN Y (IgY) MOLECULES IN AGAINST INFECTION OF Enterococcus faecalis ORIGIN OF RED TILAPIA Rifky Rizkiantino; Fachriyan Hasmi Pasaribu; Retno Damajanti Soejoedono; Wyanda Arnafia; Dinda Reisinta; I Wayan Teguh Wibawan
Jurnal Kedokteran Hewan Vol 16, No 3 (2022): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i3.24393

Red tilapia (Oreochromis hybrid) is Indonesia's leading freshwater fishery commodity susceptible to streptococcal bacterial infection. Many studies have been conducted on various efforts to prevent and treat this disease, one of which uses the immunoglobulin Y (IgY) molecule from chicken egg yolk. This study aimed to observe the biological function of IgY against Enterococcus faecalis as a cause of streptococcal-like infection. The agglutinin function was conducted by observing the growth of Enterococcus faecalis in brain heart infusion (BHI) broth media which was added with IgY suspension. The function of inhibin was performed using a spectrophotometric method to measure the level of turbidity of the bacterial suspension inoculated with IgY suspension. The bactericidal potential through the complementary activation pathway for red tilapia serum was carried out using a scanning electron microscope (SEM) method to evaluate the topography of the bacterial cell wall. The results of the study can be concluded that IgY anti-Enterococcus faecalis has the potential as an agglutinin, inhibin, and bactericidal agent through its putative potential in complement activation in streptococcal bacterial infections in red tilapia commodities.

DATE EXTRACT SUPPLEMENTATION IN RINGER LACTATE-EGG YOLK EXTENDER ON POST-THAWING QUALITY OF PELUNG CHICKEN SPERMATOZOA Hidayat, Nu'man -; Ismoyowati, Ismoyowati; Tugiyanti, Elly; Suswoyo, Imam; Rosidi, Rosidi; Sulistyawan, Ibnu Hari; Nugroho, Aras Prasetyo
Jurnal Kedokteran Hewan Vol 16, No 3 (2022): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i3.24317

The research purpose was to evaluate the influence of date extract supplementation in lactate ringer-egg yolk extender with 0.025% sodium dodecyl sulfate (LREYS) on post-thawing quality of Pelung chicken spermatozoa. Completely randomized design with 5 treatments and 4 replications was used in this study. Semen was collected from three Pelung chickens once in three days using the dorsal-abdominal massage method. Semen was divided into 5 treatment groups of date extract supplementation that were 0% as control (LREYSDE0), 0.5% (LREYSDE1), 1% (LREYSDE2), 1.5% (LREYSDE3), and 2% (LREYSDE4). The post thawing liquid semen was observed for sperm motility, viability, plasma membrane integrity and acrosome integrity. The result showed that the motility, viability, membrane plasma integrity and acrosomal integrity of spermatozoa in LREYSE extender with 1.5% date extract supplementation (30.441.02%; 49.830.91%; 43.261.02%; and 45.330.72% respectively) was significantly higher (P0.05) than others treatment that were 0% (18.210.43%; 35.620.51%; 30.540.60%; and 31.490.71% respectively), 0.5% (20.450.72%; 40.720.87%; 36.810.55%; and 38.560.63% respectively), 1% (25.680.93%; 46.270.75%; 40.521.02%; and 42.831.09% respectively) and 2% (22.550.92%; 43,710,74%; 38,840,71%; and 41,390,86% respectively) of date extract supplementation. It is concluded that the 1.5% date extract supplementation in extender can maintain the best post-thawing sperm quality.

THE EFFECTS OF SIMVASTATIN, ROSUVASTATIN, AND FENOFIBRATE ON THE BODY WEIGHT AND LIPID PROFILES OF FEMALE RATS TREATED WITH ORAL CONTRACEPTIVES AND A HIGH-FAT DIET Putri, Dwi Anggara; Djabir, Yulia Yusrini; Bahar, Muhammad Akbar; Alam, Gemini; Rahman, Latifah; Aswad, Muhammad; Arsyad, Muhammad Aryadi
Jurnal Kedokteran Hewan Vol 16, No 3 (2022): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i3.22196

This study aimed to compare the effects of simvastatin, rosuvastatin, and fenofibrate therapies on the body weight and lipid profiles of the female rats receiving a combination of oral contraceptives (COC) and a high-fat diet (HFD). Twenty female Wistar rats (130-200 g) were divided equally into four groups. The rats received a standard diet for seven days, and their baseline lipid profiles were analyzed. All rats also received COC containing 15.1 g/kg levonorgestrel/3.1 g/kg estradiol with HFD for 60 days. Starting on day 31, the rats were given the respected treatment. The control group received the vehicle, whereas the others were treated with either simvastatin (2.1 mg/kg), rosuvastatin (0.5 mg/kg), or fenofibrate (8.2 mg/kg). Blood samples were taken on days 30 and 60. The results showed that the administration of COC+HFD and vehicle for 30 days increased the rats body weight and dyslipidemia, characterized by a significant decrease in Low-Density Lipoprotein (HDL) and an increase in total cholesterol (TC) and triglycerides (TG) levels (P0.05). Among the treatments, only fenofibrate was found to be able to prevent a significant weight gain in rats. Both fenofibrate and rosuvastatin inhibited a significant rise in TG and TC levels. Meanwhile, the simvastatin administration failed to do the same. Although statistically insignificant, all treatments increased rats HDL levels. Thus, it can be concluded fenofibrate was the most effective treatment among all in reducing weight gain and improving the lipid profile of dyslipidemic rats induced by oral contraceptives and a high-fat diet.

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Tongku Nizwan Siregar

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jkh@usk.ac.id

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+626517551536

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jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Tongku Nizwan Siregar

Contact Email jkh@usk.ac.id

Phone +626517551536

Journal Mail Official jkh@usk.ac.id

Editorial Address Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location Kab. aceh besar, Aceh INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Tongku Nizwan Siregar

Contact Email
jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA