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Jurnal Kedokteran Hewan

JKH
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Published by Universitas Syiah Kuala

ISSN : 1978225X EISSN : 25025600 DOI : 10.21157

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.

Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)

Articles 901 Documents

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BRIGHTNESS-MODE EKOKARDIOGRAFI DOMBA JAWA JANTAN EKOR TIPIS Mokhamad Fakhrul Ulum; Nur Fitri Utami; Nindya Dwi Utami; Deni Noviana
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mencitrakan jantung domba jawa ekor tipis (DET) secara ekokardiografi. Domba ekor tipis jantan sebanyak lima ekor dengan berat 14-16 kg berumur 10-12 bulan digunakan dalam penelitian ini. Domba dipegang dan dibaringkan pada meja khusus tanpa sedasi ataupun pembiusan. Organ jantung domba dicitrakan menggunakan ultrasonografi brightness-mode (B-mode). Transduser cembung dengan frekuensi 2,5-4 MHz digunakan untuk mencitrakan jantung pada posisi right parasternal (RPS) view dan left parasternal (LPS) viewdengan posisi long axis (LAx) dan short axis (SAx). Hasil pencitraan ekokardiografi menunjukkan bahwa bagian-bagian dari organ jantung yang tersusun atas cairan terkesan anechoic, sedangkan bagian yang tersusun atas jaringan lunak terkesan hypoechoic dengan derajat ekogenisitas yang bervariasi. Bagian-bagian struktur jantung dapat dibedakan menurut ruang jantung atrium kanan, ventrikel kanan, atrium kiri dan ventrikel kiri. Bagian-bagian jantung yang dapat dicitrakan seperti septa jantung, otot papilaris, katup jantung semilunaris, katup jantung mitralis, katup jantung trikuspidalis, pembuluh darah vena paru-paru dan aorta. Ekokardiografi B-mode pada organ jantung domba jawa ekor tipis dapat mencitrakan dengan baik struktur ruang, otot, katup dan pembuluh darah besar dengan derajat ekogenisitas yang bervariasi.

PREVALENCE AND RISK FACTORS OF HOOKWORMS INFECTION ON DOGS IN SUKABUMI REGENCY, WEST JAVA PROVINCE, INDONESIA Yusuf Ridwan; Ardilasunu Wicaksono; Siti Vanessa Fransiska; Princesse Theresa
Jurnal Kedokteran Hewan Vol 14, No 4 (2020): December
Publisher : Universitas Syiah Kuala

Hookworms infection remains as an important parasitic infections affecting animal and human health worldwide. However, information on the prevalence and the risk factors of hookworm infection on dogs in Indonesia is very limited. A cross sectional study to estimate the prevalence and to identify risk factors of hookworms infection on dogs was conducted in rural area with different topography, Sukabumi Regency, Indonesia. A Total of 204 dog stool specimens were examined for hookworm eggs using simple flotation methods. The data of related risk factors namely type of topography area (high and low land), purpose of having dog, dog demography, dog reared management and deworming were collected through dog owners interview’s with completed a questionnaire. Those data were analyzed using the chi-square test (χ2) and logistic regression. The prevalence of hookworm on dogs in Sukabumi was 24.5 % (95% Confidence Interval [CI]=19.1-30.8). This prevalence was associated with topography of the area, the age of dog, and type of dog keeping methods. The prevalence of hookworms was higher in the high land area than low land area (OR=5.935, 95% CI=2.764-12.744 ). Logistic regression identified pupies as a high risk group to hookworms infection (OR= 2.041, 95%CI=1.035-4.055). The dog which kept in the cage/tied had higher risk than free roaming dog (OR=3.66, 95%CI=1.479-9.091).Key words: Dog, hookworms, prevalence, risk factors, Sukabumi.

APPLICATION OF ESTRUS SYNCHRONIZATION USING PGF2α AND OVULATION SYNCHRONIZATION USING hCG FOR ARTIFICIAL INSEMINATION OPTIMIZATION ON ONGOLE (PO) BREED CATTLE Mutmainnah Mutmainnah; Amrozi Amrozi; Ligaya ITA Tumbelaka
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

This study aimed to determine the pregnancy percentage of Ongole (PO) breed cattle by estrus synchronization and ovulation synchronization. This study used 22 cattle that were divided into three groups: Estrus synchronized cattle (K1, n= 5); ovulation synchronized heifers using ovsynch (K2, n= 6); and ovulation synchronized cow using ovsynch (K3, n= 11). Parameters measured were diameter of corpus luteum (CL) in estrus synchronization, follicular diameter upon synchronization and artificial insemination (AI), and percentage of pregnant cattle. Data obtained were statistically analyzed using analysis of variance followed by Duncan test. Results showed no significant differences (P0.05) of CL diameter at the time of estrus synchronization in all groups of cattle with an average of 16.63±3.79 mm. The CL diameter at the time of estrus synchronization was not significantly different among groups, with an average of 8.80 ± 2.07 mm. Diameter of follicles during ovulation synchronization was also not significantly different among groups. The average diameter of follicles was 9.01±2.05 mm. Diameter of follicles at the time of estrus and ovulation synchronization was not significantly different among groups with an average diameter of follicles of 10.94±2.10 mm. The pregnancy percentage of K1, K2, and K3 were 60%, 16%, and 36%, respectively. There was no correlation between the diameters of follicles during estrus with the pregnancy percentage. Estrus synchronization produced higher pregnancy rate than ovulation synchronization in cow or heifers.

IDENTIFIKASI PROTEIN IMUNOGENIK TAKIZOIT DAN BRADIZOIT TOXOPLASMA GONDII STRAIN LOKAL UNTUK PENGEMBANGAN KIT DIAGNOSTIK Muhammad Hanafiah; Dwinna Aliza
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Telah dilakukan penelitian untuk mengisolasi dan mengidentifikasi protein 28 kDa (GRA2) dari stadium takizoit dan bradizoit Toxoplasma gondii strain lokal. Cairan asites mencit galur Balb/c digunakan sebagai protein sampel melalui elektroforesis (SDS-PAGE), serum kambing untuk uji antigenitas protein 28 kDa (GRA2) dan serum darah kucing untuk melihat tingkat sensitivitas dan spesifisitas protein 28 kDa (GRA2). Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa protein 28 KDa (GRA2) telah dapat diisolasi dari stadium takizoit dan bradizoit Toxoplasma sp. Nilai OD405 antigenesitas ES adalah sebesar 0,578 sedangkan pada antigen GRA2 (28 kDa) diperoleh nilai OD405 adalah sebesar 0,178. Tingkat sensitivitas protein 28 kDa (GRA2) terhadap antibodi dalam serum darah kucing terinfeksi adalah sebesar 61,53% dan nilai spesifisitasnya adalah 33,33%. Tingkat sensitivitas protein ES terhadap antibodi dalam serum darah kucing terinfeksi adalah 76,92% dan tingkat spesifisitasnya adalah 33,33%.

THE DYNAMICS OF ANTIGEN SPECIFIC PROLIFERATIVE RESPONSES OF LYMPHOCYTES AT EARLY STAGES OF BOVINE PARATUBERCULOSIS INFECTION T. Reza Ferasyi; A. P. Koets; J. A. Stegeman; C. L.J.J. Kruitwagen
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): March
Publisher : Universitas Syiah Kuala

The present study was aimed to quantify the dynamics of early antigen specific proliferative responses of lymphocytes to (protein) antigens associated with experimentalMycobacterium avium subsp. paratuberculosis (Mp) infection cattle. The data were collected from20 experimentally infected calves, and 10 uninfected control animals, during the first 2 years oftheir lives. Several purified protein derivative antigens (Ppdp, Ppda, and Ppdb), tworecombinant Mp heatshock proteins (Hsp65 and Hsp70) and whole bacteria (sonicated Mpstrain 316F) were used to measure lymphocyte proliferation in a lymphocyte proliferationassay. Data were analyzed using a linear mixed effect (LME) model. The results showedsignificant group and timed effects for all antigens tested. At several time points, the responsesin the infected group were found significantly higher as compared to control group. The Ppdantigens induced similar lymphocyte proliferation patterns, as compared to whole bacteriaantigen and Hsp70. These results indicated that the antigen specific proliferative responses oflymphocytes differs for different antigens, probably related to differences in their availabilityduring different stages of infection. The application of LME model is a useful tool for analyzingthe quantitative longitudinal datasets. Keywords: dynamics, Mp, antigen, LME

DETEKSI VIRUS AVIAN INFLUENZA SUBTIPE H5N1 DI BEBERAPA PASAR UNGGAS HIDUP DALAM WILAYAH PROVINSI JAWA BARAT SEKITARNYA Atik Ratnawati; NLP Indi Dharmayanti
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Pada penelitian ini dilakukan identifikasi virus avian influenza (AI) subtipe H5N1 pada unggas dan lingkungan pasar untuk mengetahui peran pasar sebagai sumber penularan virus. Metode yang digunakan dalam penelitian ini adalah pengambilan sampel swab kloaka unggas dan lingkungan di beberapa pasar di wilayah Jawa Barat dan Tangerang. Sampel selanjutnya dilakukan isolasi ribonucleic acid (RNA) dan dilakukan reverse transcriptase polymerase chain reaction (RT-PCR) dengan menggunakan primer AI subtipe H5N1. Hasil penelitian menunjukkan bahwa virus AI/H5N1 terdeteksi pada unggas dan lingkungan pasar. Disimpulkan bahwa pasar dapat menjadi sumber penularan virus AI subtipe H5N1 terhadap unggas lainnya.

THE EFFECT OF BLACK CUMIN (Nigella sativa) SUPPLEMENTATION THROUGH DRINKING WATER ON THE HISTOLOGY OF SMALL INTESTINE AND LARGE INTESTINE OF BROILER CHICKENS Madi Hartono; Purnama Edy Santosa; Ratna Ermawati; Muhammad Mirandy Pratama Sirat
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

This study aimed to determine the effect of black cumin (Nigella sativa) supplementation through drinking water on the histology of broiler chickens’ small intestine and large intestine. The research was conducted from April-September 2020 in a cage facility of the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung. This research used a completely randomized design with four treatment groups and three replications (five broilers per replication) with a total of 60 male broilers. The treatments were drinking water without Black Cumin (P0, control); drinking water with Black Cumin 36 mg/kg BW/day (P1); 72 mg/kg BW/day (P2); and 144 mg/kg BW/day (P3). Three broilers from each group were randomly necropsied at 31 days old, and samples of the small intestine (duodenum, jejunum, and ileum) and large intestine were fixed with 10% formalin solution and sent to the Lampung Disease Investigation Center for histological preparations. The observation of preparations was carried out microscopically using the Leica DM500® Binocular Microscope to accurately calculate various parameter sizes. The results were analyzed statistically with one way Analysis of Variance at significant level 5% and if proven significant, then a Tukey test was conducted. The results of this study were that the supplementation of black cumin 72 mg/kg BW/day through drinking water could significantly increase (P0.05) 1) the average sizes of villi height and villi area of small intestine (duodenum, jejunum, ileum) and large intestine (colon) of broiler chickens; 2) the average sizes of basal villi width, villi apex width, and gland diameter of broiler duodenum; and 3) the average size of apex villi width of broiler colon. The conclusion of this study was the supplementation of black cumin at dose of 72 mg/kg BW/day through drinking water could increase the histological sizes of the small intestine and large intestine of broilers.

HISTOMORPHOMETRY OF SEMINIFEROUS TUBULES OF LOCAL GOATS FIXED WITH DIFFERENT FIXATIVES Fitriani Fitriani; Sri Wahyuni; Muslim Akmal; Dasrul Dasrul; Cut Dahlia Iskandar
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

The aim of this study was to further understand the histomorphometry of seminiferous tubules from local goat testicles that were fixed with different fixatives. Nine testicles were randomly chosen, each fixed with 10% formalin, 10% neutral buffered formalin (NBF), and 4% paraformaldehyde (PFA) solutions. The sample was then proceeded to histology preparation and stained with haematoxylin eosin (HE). Observation of the tissue was carried out using microscope and the data obtained was analyzed statistically. The results showed that there was a significant difference (P0.05) on each fixative in tubular diameter and membrane, and very significant difference (P0.01) on germinal cell and staining absorbability. Duncan test result on tubular diameter showed that the use of PFA fixative was significantly different (P0.05) compared to formalin fixative while the use of NBF fixative had no significant difference (P0.05) compared to PFA fixative. In addition, the measurement of interstitial membrane of seminiferous tubules showed no significant difference (P0.05) among three fixatives. To conclude, formalin, NBF, and PFA fixatives affect seminiferous tubules, basal membrane structure, and germinal cell appearance inside tubular lumen. Type of tissues and fixative must be considered in selecting the suitable fixative.

STRUKTUR MORFOLOGI GONAD JANTAN Geloina erosa PADA BERBAGAI UKURAN CANGKANG DI KAWASAN EKOSISTEM MANGROVE SUNGAI REULENG LEUPUNG KABUPATEN ACEH BESAR Muhammad Ali Saroeng; Razali R
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui struktur morfologi gonad jantan pada berbagai ukuran dan mengetahui pola penyebaran gonad jantan dalam kantong gonad pada waktu akan memijah. Pengambilan induk dilakukan menggunakan metode gores, sedangkan analisis laboratorium dilakukan dengan metode pembedahan. Ukuran cangkang dibagi ke dalam empat kelompok berdasarkan tingkat kematangan gonad (TKG) yaitu TKG0 berukuran ˂20 mm, TKG1 berukuran 21-29 mm, TKG2 berukuran 30-38, TKG3 berukuran 39-48, dan TKG4 berukuran cangkang ˃49 mm. Hasil yang diperoleh menunjukkan bahwa morfologi gonad jantan seperti paku dan pola penyebaran gonad jantan dalam kantong gonad secara acak dan lepas dari ikatan bersama pada muara kantong. Kesimpulan yang diperoleh adalah morfologi gonad jantan G. erosa pada TKG0, TKG1, dan TKG2 sulit dibedakan, sedangkan pada TKG3 dan TKG4 berbentuk seperti paku. Selanjutnya pola penyebaran masing-masing gonad jantan G. erosa dalam kantong gonad terjadi secara berkelompok dan acak, berada pada muara saluran gonad yang siap keluar dari kantong gonad untuk membuahi gonad betina.

DIVERSITAS GENETIK DAN HAPLOGROUP KAMBING GEMBRONG BERSTATUS KRITIS DI KABUPATEN KARANGASEM, BALI (Genetic Diversity and Haplogroup of Endangered Gembrong Goat In Karangasem, Bali) M. Syamsul Arifin Zein; Sri Sulandari; Jakaria Jakaria; I Made Londra; Suprio Guntoro; Ida Bagus Gaga Partama
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

This study was conducted to evaluate the genetic diversity and phylogeny of Gembrong goat. For this purpose, 21 goats from endangered breed in Karangasem Bali were used. Molecular analysis of genetic diversity and phylogeography used hypervariable segment 1 of mitochondrial DNA control region. The result showed that genetic variability of Gembrong goat was homogeneous with only one different sites, namely the substitution pyrimidines of C ↔ T (transitional). Phylogeny analysis results showed maternal origin of Gembrong goat is lineage (subhaplogroup) B1 with frequency of 100%. Haplogroup B were known has been domesticated from wild goat in western Asia, then headed to south Asia and infiltrated to southeast Asia, including Gembrong goat in Bali, Indonesia. As a conclusion, genetic diversity of Gembrong goat from remaining population in Karangasem very low and originate from lineages/haplogroup B1 with a frequency of 100%.

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Tongku Nizwan Siregar

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Contact Name Tongku Nizwan Siregar

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Editorial Address Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

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Abstract

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Contact Name
Tongku Nizwan Siregar

Contact Email
jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA