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Jurnal Kedokteran Hewan

JKH
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Published by Universitas Syiah Kuala

ISSN : 1978225X EISSN : 25025600 DOI : 10.21157

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.

Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)

Articles 901 Documents

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TINGKAT MATURASI IN VITRO PADA OOSIT SAPI SILANGAN SIMMENTAL PERANAKAN ONGOLE DAN LIMOUSIN PERANAKAN ONGOLE Hermilinda Parera; Agung Budiyanto
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui tingkat maturasi oosit sapi Simmental Peranakan Ongole (SimPO) dan Limousin Peranakan Ongole (LimPO) secara in vitro. Oosit dari Ovarium sapi yang berasal dari rumah potong hewan (RPH), dikelompokkan berdasarkan jenis sapi PO (kontrol), SimPO dan LimPO. Oosit diaspirasi dari ovarium dengan syringe 5 ml dan jarum 18 G. Oosit dengan kualitas A dan B yang digunakan dalam penelitian ini. Oosit dikultur dalam media maturasi TCM 199 100 µl drop dan dilapisi minyak mineral, diinkubasi pada suhu 38,5° C, CO2 5% dan kelembaban 95% selama 24 jam. Tingkat maturasi in vitro ditentukan dengan pewarnaan aceto orcein 1% untuk melihat tahapan maturasi dengan adanya perubahan konfigurasi kromosom dan membran inti berupa germinal vesicle, germinal vesicle breakdown, metafase I, anafase/telofase I, dan metafase II. Hasil penelitian menunjukkan tingkat maturasi in vitro oosit yang mencapai metafase II dari oosit sapi PO lebih tinggi secara signifikan dibandingkan sapi SimPO dan LimPO.

THE EFFECT OF Moringa oleifera LEAF EXTRACT ON THECA CELL IN POLYCYSTIC OVARY SYNDROME MODEL WITH INSULIN RESISTANCE Lisa Purbawaning Wulandari; Anindya Hapsari
Jurnal Kedokteran Hewan Vol 14, No 3 (2020): September
Publisher : Universitas Syiah Kuala

The use of Moringa oleifera as an anti-oxidant should be investigated as an alternative treatment of follicular refinement in Polycystic ovary syndrome (PCOS) with insulin resistance. We aimed to prove the effect of Moringa oleifera leaf extract in various dosages to decrease the thecacell thickness of PCOS female rat with insulin resistance. This study was a laboratory experimental research. Three month old Rattus norvegicusstrain Wistar rat weighing 100-130 g were divided into 5 groups (n= 8). PCOS model obtained by giving injection of testosterone propionate for28 days, followed by metformin therapy and Moringa oleifera leaf extract at 250 and 500 mg/kg BW for 14 days. The examination of ovariumhistology showed that leaf extract Moringa oleifera 500 mg/kg BW (0.931±0.457) significantly decreased the thickness of theca cells (P0.05)compared to the PCOS control group. The conclusion was Moringa oleifera leaf extract as an anti-oxidant proven to decrease the thickness oftheca cell of the female rat model of PCOS.

The Effects of Sulphaquinoxalin in Broiler Chicken: Clinical Symptom and Anatomical Pathology M. Nur Salim; Dian Masyitha
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

The clinical symptom and anatomical pathology effect of sulphaquinoxalin administration were studied in 12 male -seven days-old Hubbard broiler chickens with the weight of 85.57.83 g. The chickens were randomly dividedinto 2 groups of 6 each. The P0 group as control group just orally received aquadest and the P groups were treated 1 orally with 0.1% sulphaquinoxalin which dissolved in drinking water. The sulphaquinoxalin was administrated forseven days. At the end of experiment all chickens were sacrificed for necropsy. The results indicated that less appetite and drinking water as well as decrease of body weight (P0.01). Ptechial haemorrhagic was seen in thorax andfemur muscle, mucosa of duodenum, liver, kidney, heart, lung, and spleen. The liver, kidney, heart, and spleen were swollen.

INHIBIN B: KANDIDAT KONTRASEPSI PRIA BERBASIS HORMON PEPTIDA Muslim Akmal; Aulanni'am a; Muhammad Aris Widodo; Sutiman B. Sumitro; Basuki B. Purnomo
Jurnal Kedokteran Hewan Vol 5, No 1 (2011): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui efek injeksi inhibin B sebagai kandidat kontrasepsi pria berbasis hormon peptida terhadap berat badan, berat dan panjang testis, dan duktus epididimis. Sebanyak 24 ekor tikus (Rattus novergicus) jantan strain Wistar berumur 4 bulan dengan berat badan 150-200 g dikelompokkan secara acak ke dalam 4 kelompok, yaitu kontrol (KO), KI, KII, dan KIII. Kelompok kontrol, tikus hanya diinjeksi dengan 0,1 ml PBS tanpa inhibin B; Kelompok KI, KII, dan KIII tikus diinjeksi dengan 25, 50, dan 100 pg inhibin B/ekor. Injeksi dilakukan secara intra peritoneal sebanyak 5 kali dengan selang waktu 12 hari selama 48 hari. Injeksi pertama, isolat inhibin B dilarutkan dalam PBS sebanyak 0,05 ml dan diemulsikan dengan 0,05 ml Freud's complete adjuvant (FCA). Pada injeksi kedua, ketiga, keempat, dan kelima menggunakan inhibin B dalam PBS 0,05 ml dan diemulsikan dengan 0,05 ml Freud's incomplete adjuvant (FICA). Pada hari keenam setelah injeksi terakhir, tikus dikorbankan secara dislocatio cervicalis setelah terlebih dahulu dilakukan penimbangan berat badan. Berat testis dan duktus epididimis ditimbang dengan menggunakan timbangan elektrik, sedangkan diameter dan panjang testis dan duktus epididimis diukur dengan menggunakan jangka sorong. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan yang nyata (P0,05) antara kelompok kontrol dan perlakuan terhadap berat badan, berat, panjang, dan diameter testis dan duktus epididimis. Penelitian ini menyimpulkan bahwa inhibin B berpotensi dikembangkan sebagai kandidat kontrasepsi pria berbasis hormon peptida yang aman dan reversible.

DIAGNOSIS KEBUNTINGAN DINI PADA KAMBING KACANG (Capra sp.) MENGGUNAKAN ULTRASONOGRAFI TRANSKUTANEUS (Early Pregnancy Diagnosis in Dwarf Goat (Capra sp.) by Transcutaneous Ultrasonography) Arman Sayuti; Juli Melia; Ira Khubairoh Marpaung; Tongku Nizwan Siregar; Syafruddin S; Amiruddin A; Budianto Panjaitan
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

This research aimed to determine the optimum time for early pregnancy diagnosis in dwarf goat by trancutaneous ultrasonography and to determine ultrasonography imaging of dwarf goat pregnancy since insemination until embryo formation. Animal used in this research were 3 productive female goats with normal estrus cycle and 1male goat. The female goats were kept in separation pen from the male goat. The grasses were fed three times a day and additional feed were fed in the morning and afternoon with the water was given ad libitum. The goats were synchronized with intramuscular double injection of 1 ml estron®/each female goat with 11 days interval. The observation of estrus was examined using male goat following the second injection of estron®. The sign of estrus in female goats were characterized with standing immobile and allow the male goat to mount them. If the signs had been evident, the female goats were naturally mated. Pregnancy was determined by the presence of the embryonic vesicle by isoechogenic until hyperechogenic visualization surrounded by hypoechogenic of the embryonic fluid. The result of the transcutaneous ultrasonography in dwarf goat pregnancy showed the presence of embryonic vesicle on day 14 and embryo with isoechogenic visualization was detected on day 24 of pregnancy.Key words: dwarf goat, early pregnancy, transcutaneous ultrasonography

IDENTIFIKASI TOKSOPLASMOSIS PADA FESES KUCING SECARA MIKROSKOPIS DAN SEROLOGIS Wisnu Nurcahyo; Joko Prastowo; Priyowidodo P
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui tingkat prevalensi Toxoplasma pada kucing dan menyusun basis epidemiologis pada kucing yang sangat penting dalam peranannya sebagai hospes definitif toksoplasmosis. Sampel serum darah dan feses kucing digunakan dalam penelitian ini. Metode pemeriksaan menggunakan card agglutination Toxoplasma test (CATT) Pastorex™ Toxo kit dan metode sentrifus. Data yang dip eroleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa tingkat prevalensi Toxoplasma dengan pemeriksaan serum darah kucing sebesar 6,8% dan pemeriksaan feses sebesar 9,4%. Infeksi toksoplasmosis yang terjadi pada kucing secara umum dari pemeriksaan klinis tidak mempunyai gejala yang spesifik.

EFFECT OF SPECIES AND SEASON ON LAMINITIS PREVALENCE IN SEMEN-DONOR BULLS Koko Wisnu Prihatin; Jumaryoto Jumaryoto; Soedarmano Indarjulianto
Jurnal Kedokteran Hewan Vol 16, No 2 (2022): June
Publisher : Universitas Syiah Kuala

The objective of this study is to determine the effect of species, season, and their interaction on laminitis prevalence in three semen-donor bull species in the Artificial Insemination Centre under tropical conditions. The average prevalence in each species (Bos taurus, Bos indicus, and Bos sondaicus) was calculated based on the number of monthly incidents compared to the current population during 2020, while the two-way ANOVA was used to determine the effect of species, seasons (wet and dry season) and species-season interactions to the prevalence of laminitis. The average prevalence in Bos taurus, indicus, and sondaicus during dry season was 7.582±1.841%, 1.190±1.844%, 0.451±1.103%, and during wet season was 8.521±4.808%, 1.262±1.960%, 0.451±1.103% respectively. This study revealed that Bos taurus bull species more susceptible to laminitis compared to Bos indicus and Bos sondaicus. However, there is no influence of seasons and interactions between seasons and species on laminitis prevalence. In conclusion, this study has proven that laminitis can occur in all semen-donor bull species despite differences in susceptibility, species, or season.

ULTRASOUND IMAGING OF POSTPARTUM UTERINE INVOLUTION AND OVARIUM DYNAMIC IN ONGOLE CROSSBREED COWS Hazar Sukareksi; Amrozi Amrozi; Ligaya ITA Tumbelaka
Jurnal Kedokteran Hewan Vol 13, No 2 (2019): June
Publisher : Universitas Syiah Kuala

This study aimed to determine ultrasound of uterine involution and postpartum ovarian dynamics on Ongole Crossbreed Heifer (PO) associated with postpartum estrus signs. This study used 6 PO cows which were divided into primiparous group and pluripara group. The observation of uterine involution and ovarian dynamics was started from the first day postpartum using ultrasonography (USG) with a linear probe rectally, while the reproductive organs images were recorded every 2 days. The results showed that the time needed for all reproductive organs of 3 primiparous groups to complete the uterine involution after parturition was 37.33±1.15 days when the diameter of the vagina, cervix uterine, corpus uterine, left cornua, and right cornua uterine were 3.14±0.00 cm, 2.86±0.00 cm, 3.20±0.06 cm, 4.66±0.01 cm, and 4.66±0.01 cm, respectively. The time needed for all reproductive organs of 3 pluripara groups to complete uterine involution postpartum was 38.67±1.15 days with the diameter of the vagina, cervix uterine, corpus uterine, left cornua, and right cornua uterine 3.18±0.00 cm, 2.70±0.02 cm, 3.08±0.02 cm, 4.42±0.01 cm, and 4.42±0.01 cm, respectively. The average times of the first and second ovulation of primiparous cattle were 27.67±1.15 and 47.67±1.15 days postpartum, whereas in pluripara group was 28.33±1.15 and 48.33±1.15 days postpartum. At first ovulation all cows were not accompanied by signs of estrus, while at the second ovulation 2 primiparous cows and 1 pluripara cow showed less obvious signs of estrus, 1 primiparous cow and 1 pluripara cow showed signs of medium estrus, and 1 pluripara cow showed clear estrus signs.

THE EFFECTIVENESS OF THE COMBINATIONS OF CALCITRIOL AND ETHYNIL ETHYL ESTRADIOL TO DECREASE OSTEOPOROSIS AND ENDOMETRIAL CANCER RISKS IN OVARIECTOMIZED RATS Hartiningsih Hartiningsih; Devita Anggraeni
Jurnal Kedokteran Hewan Vol 11, No 2 (2017): June
Publisher : Universitas Syiah Kuala

The objective of this research was to study the effectiveness of calcitriol and ethynil ethyl estradiol combinations to decrease osteoporosis and endometrial cancer risks in ovariectomized Wistar rats. Twenty five 8-week old female Wistar rats were randomly divided into five groups (normal controlled rats/NK, ovariectomized rats/OVK, ovariectomized rats + calcitriol supplementation/OVD, ovariectomized rats + ethynil ethyl estradiol supplementation/OVE, and ovariectomized rats + calcitriol + ethynil ethyl estradiol supplementation/OVDE). At the end of the study, all rats were weighed and euthanized with 10% ketamine and 2% xylazine. Uterus and left femur were taken and fixed in 10% formaldehyde solution for histopathological examination using hematoxylin and eosin stain. Uterus was weighed before the fixation. The results showed that the percentage of uterus weight in OVK was significantly decreased compared to NK. Meanwhile, the percentage of uterus weight in OVDE was significantly increased compared to OVK. Histopathological features of the uterus in OVK were atrophy, reduction of myometrial and endometrial layers thickness, and formation of cuboidal epithelium in the endometrial lumen. However, the thickness of myometrial and endometrial layers in OVDE were increased, and its endometrial lumen was lined with metaplastic and hyperplastic squamous cell. Histopathological features of distal femur epiphysis in OVK ras showed fewer trabecular spiculums and more adipocyte in the bone marrow compared to NK. However, OVDE had more trabecular bone spiculum and less adipocyte in the bone marrow compared to OVK. In conclusion, the combination of calcitriol and ethynil ethyl estradiol supplementation could reduce osteoporosis, but increased the endometrial cancer risk in ovariectomized Wistar rats.

CELLULAR MECHANISM UNDERLYING EMBRYO-MATERNAL RELATIONSHIP IN INTRASPECIFIC AND INTERSPECIFIC PREGNANCY Diah tri widayati; Katsuhiro Fukuta
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Mouse and vole embryos were transferred into pseudopregnant CD-1 and scid female mice. Cellular changes involved in the formation of decidua in the pregnant mouse uterus up to day 8 of pregnancy were examined by histological, electron microscopic, and histochemical techniques. On day 6 of pregnancy, the vole embryos were laid in interstitium of antimesometrial side of the uterus, as well as in intraspecific pregnancy. Compared with intraspesific pregnant mouse, blood vessels were numerous in the decidua around the vole embryos in interspecific pregnancy. Both distribution and dilation of the blood vessels were increased on day 8. A part of cells in the inner cell mass had not nuclei, suggesting damaged vole embryos on day 8. At the implantation site, the uterine decidua was invaded by extravillous trophoblast (EVT) cells whose function is to destroy the walls of the uterine spiral. Moreover, this decidua was infiltrated by a population of natural killer (NK) cells and macrophage. These cells were particularly numerous in the decidua basalis at the implantation site where they come into close contact with invading EVT cells. These results suggest that interaction between NK, macrophage, and EVT provides the controlling relationship of embryo-maternal in intraspecific and interspecific pregnancy.

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Tongku Nizwan Siregar

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Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

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Home Page

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Contact Name Tongku Nizwan Siregar

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Editorial Address Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

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Abstract

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Contact Name
Tongku Nizwan Siregar

Contact Email
jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA