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Jurnal Kedokteran Hewan

JKH
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Published by Universitas Syiah Kuala

ISSN : 1978225X EISSN : 25025600 DOI : 10.21157

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.

Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)

Articles 901 Documents

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EFEKTIVITAS DIET TERI TAWAR DAN SUPLEMENTASI KALSITRIOL UNTUK MENCEGAH OSTEOPOROSIS PADA TIKUS OVARIEKTOMI The Effectiveness of Fresh Anchovies Diet and Calcitriol Supplementation to Prevent Osteoporosis in Ovariectomized Rats (The Effectiveness of Fresh Anchovies Diet and Calcitriol Supplementation to Prevent Osteoporosis in Ovariectomized Rats) Hartiningsih H; Devita Anggraeni; Sudarminto S
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

This study was conducted to assess the effectiveness of a diet contained fresh anchovies and calcitriol supplementation for 6 weeks to prevent osteoporosis in ovariectomized rats. Fifteen Wistar rats aged 8 weeks were divided randomly into 3 groups (normal/K, ovariectomized/Ov, and ovariectomized + calcitriol/OVD), 5 mice each. Group K and Ov rats were fed with fresh anchovies, while the OVD group was fed with fresh anchovies + calcitriol. At the age of 15 weeks, all mice were done for euthanasia, then left femur was collected for immunohistochemistry examination of tartrate resistant acid phosphatase5b (TRAP5b). The detection of (TRAP5b) was conducted using a monoclonal ant ibody anti TRAP5b, and detected using a streptavidin-biotin. The results showed that the metaphysis part of distal femur bone of mice group K, Ov, and OVD were positive TRAP5b stained with brown color on trabecular bone in bone marrow cavity and trabecular spiculum surface, but rats in group K and Ov had extensive bone marrow cavity and normal trabecular spiculum, whereas OVD group showed bone marrow cavity dilation, accumulation of adipocytes in the bone marrow cavity, and shorter the spiculum of trabeculae. It can be concluded that fresh anchovies diet and calcitriol supplementation for 6 weeks are not effective in preventing osteoporosis in ovariectomized rats.Key words: calcitriol, femur, ovariohisterectomy

KONSENTRASI GONADOTROPIN RELEASING HORMONE (GnRH) EKSTRAK OTAK SAPI PERANAKAN FRIESIEN HOLSTEIN BETINA FASE FOLIKULER DAN LUTEAL Nurul Isnaini; Sri Wahjuningsih
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengidentifikasi kandungan gonadotropin releasing hormone (GnRH) ekstrak jaringan otak sapi dewasa pada fase folikuler (ovari berfolikel) dan fase luteal (ovari berkorpus luteum). Pada penelitian ini digunakan 2 buah otak sapi perah peranakan Friesien Holstein (FH) dewasa dengan umur 7-8 tahun yang pada saat disembelih kondisi ovarinya menunjukkan fase folikuler (1 buah otak) dan fase luteal (1 buah otak). Analisis kandungan GnRH dilakukan dengan metode enzyme-linked immunosorbent assay (ELISA) indirect. Hasil penelitian menunjukkan bahwa konsentrasi GnRH bagian otak sapi pada fase folikuler vs fase luteal berturut-turut untuk bagian otak parietal lobe of cerebral hemisphere, corpus callosum, thalamus, hipothalamus, pituitary gland (hipofise), midbrain, cerebellum, pons, dan medulla oblongata masing-masing adalah 260 vs 270, 110 vs 170, 230 vs 320, 1590 vs 1310, 840 vs 1250, 530 vs 810, 200 vs 480, 230 vs 100, dan 220 vs 70 μg/ml. Dari hasil penelitian disimpulkan bahwa semua bagian otak sapi yang diamati mengandung GnRH dengan konsentrasi yang berbeda antara fase folikuler dengan fase luteal.

GENETIC DIVERSITY OF IRAQI LOCAL GOAT BREEDS BY RAPD DNA MARKERS Awat Nooradin Yousif; Mohammed Abdalla
Jurnal Kedokteran Hewan Vol 16, No 4 (2022): December
Publisher : Universitas Syiah Kuala

Genetic diversity of three Iraqi local goat breeds (Black, Hybrid, and Meriz) was studied by using random amplified polymorphic DNA (RAPD) markers. Fifteen primers were used, they generated a total of 259 amplification products, from which 142 bands were polymorphic, revealing 55.29% polymorphism. It was found that the mean value of Nei’s gene diversity (h) and Shannon's ınformation index (I) were 0.1778 and 0.2546, respectively. According to the analysis of genetic distances among breeds, the highest genetic distance recorded between Black and Hybrid 0.5108, while the lowest genetic distance recorded between Hybrid and Meriz 0.1431. The phylogenetic dendrogram based on Nei’s genetic distance analysis was in agreement with their phenotypic similarities in which the tree separated the surveyed breeds into two clusters, the first one including Black goat breed, and the second cluster includes Hybrid and Meriz breeds. These results indicated that Black goat breed is most genetically distant from both Hybrid and Meriz breeds. Finally, the moderate value for both of polymorphism and genetic diversity which obtained from the present study indicate that these breeds are genetically different, which provide a good background about these breeds for genetic improvement and conservation of our local goat breeds in the future.

THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO Dhesy Kartikasari; Sri Mulyati; Suzanita Utama; Pudji srianto; Widjiati Widjiati; Hani Plumeriastuti
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis. A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL. The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies. Positive results showed a brownish color on the oocyte and its cumulus. The results of this study indicated that there were significant differences (P0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P0.05) in whichthe curve of BAX/BCL-2 ratio was decreased. It can be concluded that the addition of urea does not affect the level of apoptosis.

The Use of Multiple Linear Regression Model for Predicting The Influence of Weather to The Prevalence of Newcastle Disease in Chicken in North Aceh Mahdi Abrar
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): March
Publisher : Universitas Syiah Kuala

The objective of this research is to see the influence of weather on the prevalence of Newcastle Disease (ND) in chicken in Kabupaten Aceh Utara (North Aceh). Data used in this research were obtained from Dinas Peternakan North Aceh for the number of chicken suffered ND and from Badan Meteorologi dan Geofisika Lhokseumawe, North Aceh for the form of weather. Multiple Linear Regression Model with five independent variables (the average of rainfall per month, the average of maximum temperature, the average of minimum temperature, the velocity of the wind, and the average of humidity per month) was used to see the influence of wheather to the prevalence of Newcastle Disease. Proportion the number of chicken suffered from ND which is the ratio of the number of chicken suffered from ND to the total number of chicken was used as dependent variables. The result shows that the best model is Ŷ= 120.529278 – 1.33 x wind humidity + 1.907 x wind velocity.

EFEKTIVITAS PENTAGAMAVUNON-0 TERHADAP PENGHAMBATAN EKSPRESI SIKLOOKSIGENASE-2 PADA MODEL KANKER KOLON TIKUS Risfah Yulianty; Lukman Hakim; Sardjiman S; Gemini Alam; Riska Nufika; Sitarina Widyarini
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan menentukan efektivitas pentagamavunon-0 (PGV-0) terhadap penghambatan ekspresi siklooksigenase-2 (COX-2) pada kanker kolon tikus Wistar. Pada penelitian ini digunakan 20 ekor tikus Wistar jantan yang dibagi secara acak dalam 4 kelompok perlakuan. Kelompok I merupakan kontrol negatif, kelompok II kontrol positif, kelompok III diberi PGV-0 40 mg/kg BB selama 15 minggu, dan kelompok IV diberi PGV-0 40 mg/kg BB selama 25 minggu. Pemberian PGV-0 dilakukan secara oral dua kali seminggu. Induksi kanker kolon dilakukan dengan cara injeksi subkutan DMH 60 mg/kg BB, satu kali seminggu selama 15 minggu. Pada minggu ke-26, semua hewan coba dieutanasia, kolon difiksasi dalam formalin 10% untuk selanjutnya diamati perubahan makroskopik dan mikroskopik. Penilaian ekspresi COX-2 dilakukan dengan menggunakan metode Duke’s stage dan skor imunoreaktivitas (IRS). Hasil penelitian ini memperlihatkan pemberian PGV-0 selama 25 minggu menurunkan jumlah nodul kanker kolon dari 5 ke 2 (berkurang 60%); diameter kanker kolon (pxl) dari 0,712 mm² ke 0,0043 mm² (berkurang 99,31%). Pemberian PGV-0 selama 15 minggu hanya menurunkan jumlah nodul 10% dan area kanker kolon dari 0,712 mm² ke 0,0062 mm² (99,07%). Skor imunoreaktivitas COX-2 diekspresikan oleh kelompok III dan IV adalah 4 dan 5. Gambaran histologis dari kolon mendukung hasil di atas. Pemberian PGV-0 efektif menurunkan jumlah dan area nodul kanker kolon melalui penghambatan ekspresi COX-2.

L3 Populations in Laying Hens Infected with 6,000 L2 of Ascaridia galli Darmawi D; Ummu Balqis; Risa Tiuria; Retno D. Soejoedono; Fachriyan H. Pasaribu
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

The aim of the present study was to determine the survival of L3 populations in intestine ofchickens exposed to experimental Ascaridia galli infection. Nature female adult worm were obtained fromlumen of village chickens in a comercial abattoir in Bogor. The eggs (L1) obtained from uteri female adultworms were incubated in sterile aquadestilata at room temperature for 10-20 days developed embrionatedeggs (L2). Five groups (A-D) of 80 head chickens were infected with, 6000 L2 A. galli respectively. Thechickens of group A were infected six times with dose of each 1,000 L2 with an interval of one hour. Thechickens of group B were infected three times with dose of each 2,000 L2 with an interval of two hours.The chickens of group C were infected six times with dose of each 3,000 L2 with an interval of three hours. The chickens of group D were infected one time with single dose 6,000 L2. A. galli L3 were recovered from intestines of 80 heads chickens seven days after oesophagus inoculation with 6,000 L2.The result showed that total 702,000 L1 and 628,000 L2 collected from 124 A. galli female adult worms.The percentage of L1 developed L2 is 89.46% and L2 developed L3 is 11.27%. Significant survival of L3higher populations in intestine of chickens observed only in the group D. The results indicated thatchickens infected high dose of A. galli caused the decrease of host defence against ascaridiosis. Keywords: Ascaridia galli, embrionated eggs, larvae

DETEKSI GEN tst ISOLAT Staphylococcus aureus MELALUI AMPLIFIKASI 23S rRNA ASAL SUSU KAMBING DAN SAPI PERAH Budi Prasetyo; Elizabeth Novi Kusumaningrum
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mendeteksi gen tst isolat Staphylococcus aureus (S. aureus) susu sapi perah dan susu kambing melalui amplifikasi gen 23S rRNA sebagai langkah awal pencegahan beberapa kasus keracunan susu. Metode penelitian meliputi reidentifikasi bakteri, preparasi deoxyribonucleic acid (DNA), amplifikasi gen 23S rRNA, amplifikasi gen tst, dan pengurutan DNA. Hasil reidentifikasi bakteri melalui pewarnaan Gram, uji katalase, uji koagulase, uji fermentasi MSA, uji VJA, dan uji VP adalah positif bakteri S. aureus. Hasil amplifikasi gen tst terhadap 3 isolat S. aureus menunjukkan hanya 2 isolat memberikan hasil positif. Hasil positif tersebut ditandai dengan munculnya fragmen DNA yang memiliki panjang spesifik (350 bp) sesuai dengan produk polymerase chain reaction (PCR) dari referensi dan database GeneBank. Disimpulkan bahwa dengan menggunakan metode PCR dapat terdeteksi adanya gen tst pada isolat S. aureus asal susu sapi perah dan susu kambing dari Bogor.

IDENTIFIKASI Escherichia coli O157:H7 DARI FESES AYAM DAN UJI PROFIL HEMOLISISNYA PADA MEDIA AGAR DARAH I Wayan Suardana; Iwan Harjono Utama; Michael Haryadi Wibowo
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan melakukan isolasi dan identifikasi serotipe lokal Escherichia coli (E. coli) O157:H7 dan uji profil hemolisisnya pada media agar darah. Isolasi bakteri dilakukan dengan media eosin methylene blue agar (EMBA), dilanjutkan dengan identifikasi pada media selektif sorbitol MacConkey agar (SMAC) dan uji konfirmasi menggunakan uji aglutinasi lateks O157 serta uji antiserum H7 sebagai konfirmasi akhir dari E. coli O157:H7. Gambaran hemolisis diuji dengan menumbuhkan isolat pada media agar darah domba. Hasil penelitian menunjukkan bahwa 7 isolat (8,54%) dari 82 sampel feses ayam teridentifikasi E. coli O157:H7 dan memperlihatkan profil enterohemolisis seperti halnya isolat kontrol ATCC 43894. Dari hasil penelitian disimpulkan bahwa isolat lokal E. coli O157:H7 hasil isolasi dari feses ayam diketahui memiliki patogenitas yang tinggi terkait dengan dihasilkannya enterohemolisin yang merupakan marka/penanda kemampuan dari isolat untuk menghasilkan faktor virulensi Shiga like toxin.

SENSITIVITY OF Microsporum canis ISOLATED FROM DOGS TOWARDS ANTIFUNGALS Gerson Yohanes I Sakan; Soedarmanto Indarjulianto; Alfarisa Nururrozi; Yanuartono Yanuartono; Slamet Raharjo
Jurnal Kedokteran Hewan Vol 14, No 4 (2020): December
Publisher : Universitas Syiah Kuala

The dermatophytosis treatment in dogs can fail due to dermatophyte resistance to antifungals. The purpose of this study is to determine the antifungal sensitivity of dermatophyte Microsporum canis (M. canis) isolated from dogs with dermatophyte. A total of 17 M. canis dog isolateswere tested for sensitivity to 25 µg fluconazole, 25 µg griseofulvin, 15 µg ketoconazole and 30 µg terbinafine, using the disc diffusion method.The M. canis isolates were cultured on Sabouraud's Dextrose Agar media, placed an antifungal disc, and then incubated at 28 C for 7 days. Thediameter of inhibition zone was measured, then compared with the standard, to determine whether they were sensitive, intermediate or resistant.The results of this study indicate that 16 isolates were sensitive to fluconazole; 17 isolates were sensitive to griseofulvin, ketoconazole andterbinafine; and one isolate was intermediate to fluconazole. Based on these results, it is concluded that all 17 (100%) isolates are sensitive to theantifungals griseofulvin, ketoconazole, terbinafine, and 16 (94%) isolates are sensitive to fluconazole. Thus, the four antifungals can be selectedto treat dermatophytes in dogs.

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Tongku Nizwan Siregar

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Editorial Address Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

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Contact Name
Tongku Nizwan Siregar

Contact Email
jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA