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Obat: Jurnal Riset Ilmu Farmasi dan Kesehatan
Published by Asosiasi Riset Ilmu Kesehatan Indonesia
ISSN : 3031013X     EISSN : 30310148     DOI : 10.61132
Core Subject : Health,
Health Professions Nursing Public Health
Organisasi Farmasi, Kedokteran, Kimia Organik Sintetis, Kimia Alam, Biokimia, Analisis Kimia, Kimia Fisika, Biologi, Mikrobiologi, Jaringan Budaya, Botani dan Hewan terkait Produk Farmasi, Keperawatan, Kebidanan, Analis Kesehatan, Gizi dan Kesehatan Masyarakat.
Arjuna Subject : Profesi Kesehatan - Farmasi
Articles 352 Documents
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Extraction, Optimization, and Antioxidant Evaluation of Arginine Deiminase Enzyme in the Escherichia Coli Clinical Isolates Jawad N. K. Makassees
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 3 No. 5 (2025): September : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v3i5.1633

Abstract

Arginine deiminase (ADI) is a promising enzyme with significant therapeutic potential, particularly for its anticancer effects through the depletion of arginine in cancer cells that are auxotrophs. In this study, we aimed to optimize the production of ADI using clinical Escherichia coli isolates and to evaluate its antioxidant activity. A total of 25 E. coli isolates were obtained from 45 hospital samples collected in Wasit Province, Iraq. Optimization of ADI production was performed by systematically testing various factors including culture media, pH, carbon and nitrogen sources, incubation temperature, and time. The antioxidant activity was assessed using the DPPH radical scavenging assay. The highest ADI production was achieved using a modified M9 medium supplemented with 1% w/v sucrose as the carbon source and 5% w/v yeast extract as the nitrogen source. The optimal enzyme activity of 1.6 U/mg protein was observed at pH 7.0, 37°C, and after 24 hours of incubation. The crude ADI extract exhibited high antioxidant activity, with 79.28 ± 1.06% DPPH scavenging at 200 µg/mL, comparable to ascorbic acid, which showed 86.11 ± 1.45% DPPH scavenging. The study successfully optimized the conditions for enhanced ADI production based on clinical E. coli isolates, demonstrating its potential as both an anticancer enzyme and an antioxidant. The dual therapeutic potential of ADI warrants further research, including clinical trials, to explore its application in cancer therapy and as an antioxidant in medical treatments, offering promising avenues for future drug development and improved therapeutic strategies, particularly for targeting cancer and oxidative stress-related diseases.
Pengaruh SPF terhadap Stabilitas dan Efektivitas Lip Balm Alami dari BEESWAX dan Minyak Kelapa Naifah Nahda; Amraini Amelia
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 3 No. 5 (2025): September : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v3i5.1634

Abstract

The lips are one of the most sensitive parts of the body and are highly vulnerable to damage from ultraviolet (UV) radiation from sunlight. Unlike other parts of the skin, the lips lack oil glands, making them more prone to dryness, chapping, and inflammation when not properly protected. One common method to maintain lip moisture and health is the use of lip balm. Natural lip balms made from ingredients such as beeswax and coconut oil are widely used due to their excellent emollient properties, which help moisturize and provide a protective layer on the surface of the lips. However, using natural lip balm alone may not be sufficient to offer complete protection, particularly against the harmful effects of UV rays. Therefore, innovation is needed by incorporating Sun Protection Factor (SPF) into natural lip balm formulations to enhance protection from sun exposure. This study aims to evaluate the effect of adding SPF to lip balm formulations based on beeswax and coconut oil, focusing on UV protection effectiveness, product stability, and moisturizing ability. The method involved formulating two types of lip balm—one with SPF and one without. UV protection effectiveness was tested using in vitro spectrophotometry, while moisturizing ability was assessed by measuring skin moisture content on the lips. In addition, physical characteristics such as texture, color, and stability under various storage conditions were analyzed. The results showed that the lip balm with added SPF provided significantly higher UV protection compared to the formulation without SPF. Furthermore, the addition of SPF did not reduce the moisturizing performance of beeswax and coconut oil and did not interfere with the overall product stability. In conclusion, integrating SPF into natural lip balm formulations is an effective innovation to enhance the protective function of lip care products without compromising their moisturizing benefits or user comfort. This combination provides a holistic solution for maintaining lip health while preventing UV-induced damage.
Formulasi Dan Uji Stabilitas Fisik Lip Balm Alami Berbahan Dasar Cera Flava Untuk Perlindungan Bibir Pada Praktikum Farmasetika Dea Lestari; Amraini Amelia
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 3 No. 5 (2025): September : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v3i5.1638

Abstract

Lip balm is a cosmetic product designed to protect and maintain the moisture of the lips, especially from environmental factors that can cause dry, cracked, or irritated lips, such as low temperatures and wind. Dry lips can interfere with comfort and appearance, making protection with lip balm essential. This study aims to formulate and evaluate a lip balm preparation made from a combination of natural and chemical ingredients, such as cera flava, oleum cacao, nipagin, glycerin, lanolin, and the addition of powder dye. These ingredients were chosen because of their ability to maintain lip moisture, provide protection against external factors, and ensure long-term safety for use. The lip balm manufacturing process begins with heating the primary ingredients, cera flava and oleum cacao, which are melted first. After the primary ingredients have melted, additional ingredients such as nipagin, glycerin, lanolin, and powder dye are added gradually. The mixture is stirred carefully to ensure that all ingredients are evenly blended. Once all the ingredients are well-mixed, the liquid mixture is poured into molds and allowed to cool and harden. This process is crucial for achieving the desired lip balm texture, which should not be too hard or too soft and should facilitate easy application to the lips. Based on the analysis results, the formulated lip balm demonstrates good physical stability, without significant changes in color, odor, or texture during testing. The lip balm also provides ease of use, with a smooth texture that is easy to apply to the lips. The results of this study show that the combination of ingredients used can produce an effective lip balm product that maintains lip moisture and provides optimal protection against harsh environmental conditions, such as low temperatures.
Ramuan Ekor Naga vs. Obat Sintetis : Studi Kontemporer tentang Potensi Analgesik pada Hewan Mencit Haryanto Haryanto; Alya Nurul Pertiwi; Ummu Aidah; Andi Alisa Alsa; Adinda Maharani; Sumiati Sumiati; Ratri Pramudita; Resti Resti
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 3 No. 5 (2025): September : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v3i5.1649

Abstract

Pain is an unpleasant sensation that can interfere with an individual's quality of life and requires effective management. Synthetic analgesics such as ketorolac, codeine, etoricoxib, and celecoxib are commonly used to relieve pain, but long-term use can cause adverse side effects, including gastrointestinal disturbances, dependence, and cardiovascular risks. Therefore, it is important to find safer, natural alternatives. One promising alternative is the extract of the dragon’s tail leaf (Rhaphidophora pinnata), a plant traditionally used in Indonesian medicine as a pain reliever. This study aims to evaluate and compare the analgesic effects of Rhaphidophora pinnata leaf extract with several synthetic drugs using the writhing test on male mice (Mus musculus). The mice were induced with pain by injecting a 0.5% acetic acid solution. The treatments included ketorolac, codeine, etoricoxib, celecoxib, and Rhaphidophora pinnata leaf extract. The results showed that all treatments, including both synthetic drugs and Rhaphidophora pinnata leaf extract, produced significant analgesic effects compared to the negative control (Na CMC 0.5%). Ketorolac showed the highest effectiveness, followed by codeine, etoricoxib, and celecoxib. Although the Rhaphidophora pinnata leaf extract showed potential as a natural analgesic, its effectiveness varied among individual test animals. Some mice showed a better response to the leaf extract, but overall, the analgesic effect was still lower than that of synthetic drugs. These findings support the use of medicinal plants as a safer alternative to synthetic analgesic drugs. Moreover, this study provides a foundation for further research aimed at isolating active compounds from Rhaphidophora pinnata leaf extract to develop more effective and safer pain-relieving medications.
Evaluating Patients' Knowledge about Lymph Node Cancer and Its Relationship to the Family's Genetic History in the Cancer Center Iqbal Sahi Tuama; Qassim Jawell Odah Abed; Rana Ali Hameed
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 3 No. 5 (2025): September : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v3i5.1640

Abstract

Lymphoma is a type of cancer that begins in the lymphatic system, which is part of the body's immune system. The lymphatic system consists of lymph nodes (lymph nodes), lymph vessels, the spleen, bone marrow, and other organs. Lymphoma occurs when lymphocytes multiply abnormally. Lymphoma is one of the most common cancers, affecting approximately 8 million people worldwide annually. These statistics should not be underestimated, but should be taken into consideration. This study aimed to assess the level of knowledge of patients with lymphoma and to investigate the relationship between family history and risk of developing the disease.  A descriptive and analytical cross-sectional study was conducted at a final center in Wasit Governorate, Iraq, from January 2024 to March 2025. Forty patients were included in the study, who were diagnosed with lymphoma and had their lymph nodes sorted. Data were collected through a structured questionnaire covering demographics, family history, and knowledge about the disease. Data were analyzed using SPSS and Excel, with descriptive statistics and chi-square testing applied to determine statistical relationships (at a significance level of ≤ 0.05). The study was conducted using a descriptive analytical approach at the Oncology Center in Wasit Governorate, Iraq, from January 2024 to March 2025. The sample included 40 female patients diagnosed with lymphoma. Data were collected through a structured questionnaire covering demographics, family history, and knowledge about the disease. Data were analyzed using SPSS and Excel, using descriptive statistics and the chi-square test to determine statistical relationships (at a significance level of ≤ 0.05). Most of the participants (61%) had poor knowledge of lymphoma, particularly about its signs and symptoms, risk factors, and preventive measures.
The Involvement of miRNA-375, miRNA-451, and IL-6 in the Immunopathogenesis of Hashimoto's Thyroiditis and Its Implications Bashar A. Hadad; Wejdan Thamir Mahdi
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 3 No. 5 (2025): September : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v3i5.1650

Abstract

Hashimoto's thyroiditis (HT) is an autoimmune disorder that leads to thyroid dysfunction and chronic inflammation, often resulting in hypothyroidism. The exact mechanisms driving HT are not fully understood, but recent research has highlighted the potential role of various molecular markers in the disease’s pathogenesis. This study investigated the roles of miRNA-375, miRNA-451, and interleukin-6 (IL-6) in HT. Serum samples were collected from 100 HT patients and 100 healthy controls, and their expression levels were analyzed using quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA). The results revealed significant alterations in the levels of these molecules. miRNA-375 was significantly elevated in HT patients (5.85 ± 1.30 vs. 1.0, p < 0.001), while miRNA-451 was markedly reduced (1.43 ± 0.22 vs. 10.91 ± 2.44, p < 0.001). Additionally, IL-6 levels were found to be significantly higher in HT patients (115.79 ± 12.62 vs. 21.34 ± 4.03 pg/ml, p < 0.001). These findings suggest that these molecules play critical roles in the immunopathogenesis of HT. Receiver operating characteristic (ROC) analysis further confirmed that miRNA-375, miRNA-451, and IL-6 are excellent diagnostic markers, with area under the curve (AUC) values ranging from 0.956 to 1.000. miRNA-375 showed 96% sensitivity and specificity at a cutoff >1.66-fold, while miRNA-451 demonstrated 100% sensitivity and specificity at a cutoff <3.48-fold. IL-6 also exhibited perfect diagnostic accuracy with 100% sensitivity and specificity at a cutoff >53.73 pg/ml. No significant variations were observed across demographic or treatment subgroups, supporting the stability of these biomarkers in clinical applications. These findings suggest that miRNA-375, miRNA-451, and IL-6 are dysregulated in HT and could serve as reliable biomarkers for diagnosis and potential therapeutic targets, offering new strategies for the management of Hashimoto’s thyroiditis.
Efektivitas Antikonvulsan Glodokan Tiang (Polyathia longifolia) pada Hewan Uji Mencit yang Diinduksi Strikinin Haryanto Haryanto; Nur Fadila Rustam; Nur Amalinda; Hardianti Hardianti; Fitrinaya Fitrinaya; Nur Halisah
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 3 No. 5 (2025): September : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v3i5.1669

Abstract

Epilepsy is a chronic neurological disorder characterized by recurrent seizures due to abnormal electrical activity in the brain. This condition can significantly impact the quality of life of sufferers, making the development of effective and safe anticonvulsant therapies a priority in pharmacological research. This study aimed to evaluate the effectiveness of various anticonvulsant drugs, including herbal candidates, in a mouse model (Mus musculus) using acute and subchronic test protocols. Seizures were induced by administering strychnine, a glycine receptor antagonist that triggers excessive neuronal excitability. Parameters observed included seizure onset (the time from strychnine administration to the onset of the first seizure) and deadtime (the time of survival after a seizure). The study involved six treatment groups: a negative control (Na-CMC), four conventional drug groups (phenytoin, diazepam, pregabalin, and gabapentin), and one herbal candidate group made from Polyalthia longifolia (glodokan tiang). The results showed that most treatment groups were able to delay seizure onset and significantly prolong deadtime compared to the negative control. Statistical analysis using ANOVA yielded a p-value <0.05, indicating a significant difference between groups. The herbal candidate Polyalthia longifolia showed promising anticonvulsant potential, although it did not fully match the effectiveness of conventional drugs such as phenytoin or diazepam. The negative control (Na-CMC) showed the fastest onset and shortest survival time, reflecting the absence of a protective effect against seizures. This study emphasizes the importance of further testing using chronic protocols and evaluating additional parameters such as plasma drug concentrations and neurological side effect profiles. The obtained data are expected to form the basis for the development of anticonvulsant therapies based on a combination of modern drugs and natural ingredients, with the hope of producing safer, more effective treatment alternatives, and potentially reducing dependence on long-term synthetic drugs.
Review: Sterilisasi Produk Farmasi Steril dengan Metode Through Flow Filtration dan Tangential Flow Filtration I Gede Yudistira Perdangga Bandem; Kadek Adisthi Pradipthasari; Kadek Indra Aryani; Ni Luh Dian Senja Pratiwi; Putu Siska Angelina Pramesti; I Gusti Ngurah Agung Dewantara Putra
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 3 No. 5 (2025): September : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v3i5.1675

Abstract

The production of pharmaceutical sterile products requires strict procedures to minimize the risk of microbial, particulate and pyrogen contamination that can be harmful to the user's health. The selection of the right sterilization method is the key to achieving the desired stability of the final product. Filtration sterilization is one of the oldest methods used for pharmaceutical sterile products. There are two types of feed flow in the method, namely through flow filtration and tangential flow filtration. This review article is prepared through literature search and download of national or international journals with inclusion or exclusion criteria that have been set. Literature sources are accessed online from various sites such as Google Scholar and Pubmed in the range of 2014-2024. The keywords used for journal searches are sterilization, through flow filtration, and tangential flow filtration. This review article reviews and compares the two methods to understand the differences, mechanism of action, and research objectives based on the review article on membrane filtration sterilization. This review aims to provide a better insight into the advantages and disadvantages of each membrane filtration sterilization method, as well as guidance in choosing the appropriate method based on the characteristics of the product to be sterilized. The results showed that through flow filtration is more commonly used in small volume and batch processes because of its simple design, but has limitations in reducing membrane fouling. In contrast, tangential flow filtration allows for continuous processing of larger volumes, with the advantage of minimizing particle build-up on the membrane surface, thus extending membrane life. The choice of method is also influenced by factors such as particle size, viscosity of the solution, sensitivity to heat, as well as operating costs. An in-depth understanding of the Working Principle and application of each method is essential for the pharmaceutical industry to ensure the quality, safety and efficiency of the production process. Thus, this review is expected to be a reference for researchers and practitioners in determining the optimal filtration sterilization strategy according to product needs.
Computational Study of (E)-4-hydroxy-3-(phenylamino)pent-3-en-2-one as A Novel Organic Corrosion Inhibitors Aseer shakir Ajel
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 3 No. 5 (2025): September : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v3i5.1685

Abstract

This study investigates the corrosion inhibition potential of a newly synthesized organic compound, (E)-4-hydroxy-3-(phenylamino)pent-3-en-2-one (LASA3), using computational chemistry approaches. Density Functional Theory (DFT) calculations were performed at the B3LYP/6-31G(d) level of theory with the Gaussian09 software package to evaluate several key quantum chemical parameters. These parameters include total energy, the energies of the highest occupied molecular orbital (EHOMO) and lowest unoccupied molecular orbital (ELUMO), the energy gap (ΔEgap), dipole moment, chemical hardness, softness (σ), and the number of electrons transferred (ΔN). The computational results reveal that LASA3 exhibits a higher EHOMO value and a smaller ΔEgap compared to its precursor molecules, referred to as S.M.1 and S.M.2. A higher EHOMO value suggests that LASA3 has a greater electron-donating ability, which enhances its interaction with the metal surface. Likewise, the reduced ΔEgap indicates greater chemical reactivity and a higher likelihood of forming stable coordination bonds with iron atoms on the carbon steel surface. Electrostatic potential (ESP) map analysis further supports these findings by highlighting the distribution of electron density within the LASA3 molecule. The ESP maps show significant electron-rich regions localized around nitrogen and oxygen atoms, which are potential active sites for adsorption onto the steel surface. This adsorption process plays a crucial role in blocking active corrosion sites and reducing the rate of metal degradation. In conclusion, the theoretical analysis confirms that LASA3 has superior electronic properties for corrosion inhibition compared to its starting materials, S.M.1 and S.M.2. Its ability to donate electrons, favorable dipole characteristics, and strategically located electron-rich sites make it a promising candidate for further experimental evaluation as an efficient corrosion inhibitor for carbon steel applications.  
Biological effect of Silver Nanoparticles synthesis by Leaves of Cymbopogon citratus on eggs and larvae of Cadra cautella (Walk.) (Lepidoptera: Pyralidae) Sarah Ibrahim Mahmood
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 3 No. 5 (2025): September : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v3i5.1690

Abstract

During storage and before consumption, grains often experience a decline in quality, nutritional content, and cleanliness due to pest attacks. Botanical insecticides are considered an alternative method to reduce dependence on harmful chemical pesticides. This study aimed to evaluate the effectiveness of three concentrations of silver nanoparticles (AgNPs) synthesized from the leaves of Cymbopogon citratus against Cadra cautella (Walk.) (Lepidoptera: Pyralidae) and their potential as biological control agents. The selection of C. cautella as the test organism was based on the limited number of studies examining this pest. The AgNPs used were synthesized through an eco-friendly method, and their optical and physical properties were analyzed using UV-Vis spectroscopy. The results demonstrated that AgNPs synthesized from C. citratus extract showed high effectiveness in controlling the eggs and second instar larvae of C. cautella. Egg mortality reached 80.3% at a concentration of 40 μg/ml after 24 hours of exposure, followed by 72.9% at 30 μg/ml, and 65.8% at 20 μg/ml. In contrast, the control group showed no egg mortality. Similarly, larval mortality rates were 74.9%, 65.8%, and 60.2% for concentrations of 40 μg/ml, 30 μg/ml, and 20 μg/ml, respectively. These findings indicate that higher concentrations of AgNPs lead to greater mortality in both eggs and larvae. This study highlights the potential of green-synthesized AgNPs from C. citratus leaves as an effective and eco-friendly biopesticide. Their significant impact on egg and larval mortality suggests that they can be developed as an alternative pest control strategy in stored grains, thereby reducing reliance on conventional chemical pesticides that are detrimental to human health and the environment. Further research is recommended to explore their long-term efficacy, safety, and integration into sustainable grain storage management.

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