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Jurnal Agro Veteriner
Published by Universitas Airlangga
ISSN : 23031697     EISSN : 30319811     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : Kedokteran Hewan - Food Animals
Articles 36 Documents
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PCR-based embryo sex-determination assay in cattle based on the amelogenin gene Mengistie, Dejenie; Kelbessa, Shelema; Seyoum, Betelhem; Arega, Bezina; Ali, Sayid; Dire, Mosisa; Girmay, Gebrerufael; Bedassa, Abdi; Tegegne, Desiye Tesfaye
Jurnal Agro Veteriner Vol. 9 No. 1 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v9i1.75260

Abstract

The amelogenin gene-based polymerase chain reaction (PCR) assay offers a precise, efficient, and cost-effective approach for embryo sex determination in cattle. This method capitalizes on genetic differences between the X and Y chromosome-linked amelogenin genes, enabling early sex identification critical for livestock management. Our study outlines the methodology, from embryo collection and DNA extraction to PCR amplification and gel electrophoresis analysis. Results consistently demonstrated the assay's reliability, identifies that all examined embryos were female embryos (X/X) across developmental stages with a male and female control (X/Y) (X/X) respectively, validating the technique. This study underscores the assay’s potential for advancing selective breeding and enhancing productivity in the cattle industry.
Ocular Surface Microbiome Transplantation In Dogs: Treat To Target On ‘Gut-Eye Axis’: Gut-Eye Axis-Based Ocular Microbiome Therapy in Dogs Ural, Kerem; Erdogan, Hasan; Erdogan, Songül; Pasa, Serdar; Ozalp, Tahir
Jurnal Agro Veteriner Vol. 9 No. 1 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v9i1.76639

Abstract

The ocular surface microbiome (osum) is an organized group of microorganisms along with owned genes residing on eye surface. The latter collection is a normal trait for eye health with a participant protection role. In the present original article of prospective case series the presenting author aimed at reporting natural treatment with osum, as because of microbiome modulation of ‘treat to target’ purpose. Given disadvantages of drugs used in ophthalmology, and based on selected probiotic strains with a very limited choices, presenting author decided to change ocular surface microechology by transferring microbiome from healthy donors to diseased 7 dogs. Canine allergic conjunctivitis (n=3), n=1 each for Canine Visceral Leishmaniasis co-morbidity infectious ulcerative keratitis, keratoconjunctivitis sicca, Canine Monocytic Ehrlichiosis related infectious ulcerative keratitis and vision loss were entire problem list. On every session a healthy donor was selected and used for transfer of ocular surface microbiome by use of sterile cotton swab.Complete recovery (n=3) and partial recovery (n=1) were evident out of 7 dogs enrolled. This natural treatment modality, unless this technique was developed by the presenting author, could substitute drug usage at least for canine allergic conjunctivitis along with microbiome modulation
Effects of Captivity on the Morphology, Nesting Success, and Growth of the Libyan Falcon (Falco biarmicus) Dakheel, Husam
Jurnal Agro Veteriner Vol. 9 No. 1 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v9i1.77626

Abstract

Understanding raptor breeding biology is essential for conservation. This study assessed the captive breeding of the Lanner Falcon (Falco biarmicus) and raptor presence in Libya. A pair acquired in 2018 was provided with artificial nests and monitored throughout the 2024 season. Clutch size ranged 4–6 eggs with a 32-day incubation. In 2024, all eggs hatched, producing three fledglings independent after four months. Between 2018–2025, the pair laid 35 eggs, yielding about 25 fledglings, confirming high captive success. Field surveys in October–November 2024 recorded 53 raptors: Falco peregrinus (39; Least Concern), F. biarmicus (6; resident), and Falco cherrug (8; Endangered, migratory). F. peregrinus was the most frequent, with Al Kufrah identified as the main hunting area. Results highlight the effectiveness of captive breeding for Lanner Falcons and underline urgent threats to wild populations, particularly habitat loss and poaching. Strong conservation actions are critical for sustaining both resident and migratory raptors.
Description of antemortem inspection of cattle at the Dimoro slaughterhouse, Blitar City Hasibuan, Sandi Aziz; Lokapirnasari, Widya Paramita; Triakoso, Nusdianto; Budiarto, Budiarto; Warsito, Sunaryo Hadi; Raharjo, Dadik
Jurnal Agro Veteriner Vol. 9 No. 1 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v9i1.78414

Abstract

The outbreaks of Foot and Mouth Disease (FMD) and Lumpy Skin Disease (LSD) in early 2024 significantly impacted livestock health conditions in Blitar City and led to an increase in emergency slaughter cases. The Dimoro Slaughterhouse plays a crucial role in ensuring the safety of animal-derived food through the implementation of antemortem inspections. This study aims to evaluate the health status of beef cattle and identify pathological conditions detected during antemortem inspections at the Dimoro Slaughterhouse. The research used a quantitative descriptive method with direct observation of 40 cattle during the period of June 13–19, 2025. Clinical examinations were conducted on the head, neck, thorax, abdomen, anus, and limbs. The results showed that most cattle were male (34 heads) and predominantly of the Limousin breed (19 heads). No clinical symptoms of FMD, LSD, fever, or other disorders warranting slaughter rejection were found. Mild findings such as dirt around the anus were recorded in some individuals. Overall, the antemortem inspection was carried out effectively and contributed to ensuring the safety of meat products for public consumption.
The effect of laserpuncture shooting on the histomorphometry of the bursa of Fabricius in pullets (Gallus gallus) that lay eggs late Tiarani, Ayu Kamila; Lokapirnasari, Widya Paramita; Soeharsono, Soeharsono
Jurnal Agro Veteriner Vol. 9 No. 1 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v9i1.78449

Abstract

The continuing increase in egg consumption has led to the need to ensure that the laying hen industry sector is able to keep up with the need for eggs, one way is by increasing the productivity of laying hens and ensuring that laying hens can lay eggs on time. This study aims to determine the number and diameter of the bursa of Fabricius lymphoid follicles in late-laying pullets laserpuncture. This research is a 2x4 factorial complete randomized design experimental study. Chickens were divided into two groups, First Grade and Second Grade. Each Grade group was further divided into four groups based on laserpuncture points, namely control, Ova, weigen, and Ova-weigen. The variables observed were the diameter and number of lymphoid follicles of the bursa of Fabricius microscopically. The observation data were analyzed statistically using analysis of variance (ANOVA) followed by the Duncan test. The results of the analysis showed that 0.5 Joule laserpuncture had a significant effect on the diameter and number of the bursa of Fabricius lymphoid follicles (p<0.05). Laserpuncture shooting 0.5 Joules at the Wei Gen point shows the smallest diameter of the lymphoid follicles. While Ova point showed the smallest number of bursa Fabricius lymphoid follicles.
Detection of Salmonella sp. in broiler chickens in closed houses using the polymerase chain reaction method Larasati, Anissa Dhea; Sabdoningrum, Emy Koestanti; Nidom, Chairul Anwar; Hidanah, Sri; Rachmawati, Kadek; Puspitasari, Yulianna
Jurnal Agro Veteriner Vol. 9 No. 1 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v9i1.78646

Abstract

This study aimed to detect the presence of Salmonella sp. in broiler chickens raised in closed house systems using the Polymerase Chain Reaction (PCR) method. Liver and intestinal samples were collected from chickens showing clinical signs such as diarrhea, anorexia, and lethargy, along with pathological lesions observed during necropsy. Bacteriological identification involved isolation using selective media, Gram staining and biochemical tests. Two samples (3A and 3B) showed colony morphology and biochemical characteristics consistent with Salmonella sp. Confirmation using PCR targeting the invA gene (primers 139 and 141, expected amplicon size 284 bp) yielded negative results in both samples, while the positive control successfully amplified the target. The inconsistency between bacteriological and molecular results may be attributed to several factors: absence or mutation of the invA gene in the tested isolates, primer mismatch with local Salmonella strains, or inadequate DNA quality and concentration. These findings suggest that reliance on a single molecular marker may be insufficient for accurate detection and underscore the importance of optimizing PCR conditions. The study highlights the need for locally adapted primers and complementary diagnostic approaches to improve the reliability of Salmonella sp. detection in poultry, particularly in intensive production systems like closed house environments.

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