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All Journal El-Hayah : Jurnal Biologi Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease Jurnal Layanan Masyarakat (Journal of Public Service) journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Jurnal Agro Veteriner
Raharjo, Dadik
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Antimicrobial Activities of Laurus nobilis Leaves Ethanol Extract on Staphylococcus aureus, Salmonellae typhi, and Escherichia coli. Sakran, khawla Abdullah; Raharjo, Dadik; Mertaniasih, Ni Made
Indonesian Journal of Tropical and Infectious Disease Vol. 9 No. 2 (2021)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v9i2.22873

Abstract

Laurus nobilis is one of the most well-known, most frequently used plants is from Lauraceae family which contains up 2.500 species that grow in the subtropics and tropics of the Mediterranean region and Indonesia. This study was supposed to investigate the antimicrobial eff ect of L.nobilis leaves ethanol extract on Staphylococcus aureus, Salmonellae typhi, and Escherichia coli. This preliminary study examined the antimicrobial eff ect of L.nobilis leaves ethanol extract. The method used Agar-well diff usion for determination of the zone of inhibition and the minimum bactericidal concentration to investigate the activity of L.nobilis leaves ethanol extract at 100% concentration . The results revealed that extract of L. nobilis leaves had the antibacterial activity against Staphylococcus aureus with a zone of inhibition (16.3 ±1.5 mm), Staphylococcus aureus with (14.5±0.5 mm), and weak antimicrobial activity against Escherichia coli (11.3±1.1mm). Also, through the minimum bactericidal concentration experiment, the L.nobilis leaves ethanol extract had activity on Staphylococcus aureus and Salmonellae typhi, it's killed the bacteria in all concentration start it from 5í—107 to 5í—104. But the activity on Escherichia coli just weaken concentration 5í—107 and 106. This research has concluded that the L.nobilis leaves ethanol extract exhibited a signifi cant antimicrobial eff ect against Staphylococcus aureus and Salmonellae typhi then Escherichia coli that is considered a kind of multidrug-resistant bacteria.
Total Plate dan Total Staphylococcus aureus pada Daging Di Pasar Tradisional Kecamatan Mulyorejo Surabaya Mutiarasari, Nonie Olivia Adia; Harijani, Nenny; Rantam, Fedik Abdul; Raharjo, Dadik; Estoepangestie, Agnes Theresia Soelih; Handijatno, Didik
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28584

Abstract

This study aimed to evaluate the Total Plate Count and total Staphylococcus aureus count of beef sold in wet markets in Mulyorejo sub-district below the National Standard Indonesia (SNI 7388:2009) about maximum limit of microbial contamination in food or not. Total of twenty four samples of beef purchased from traditional markets of Tempurejo, Krempyeng Yamuri, Pacar Keling, and Menur in Mulyorejo sub-district Surabaya were examined by Total Plate Count using pour plate method. The sample was also cultured in Mannitol Salt Agar. The colony suspected to be S. aureus were taken for identification. The identification of S. aureus consists of isolation in Mannitol Salt Agar, Gram staining, catalase test, and coagulase test. Total plate count result showed that four samples were exceeding the National Standard of Indonesia SNI 7388:2009 or 1x106 CFU/g and the rest were below the maximum Total Plate Count in SNI. The highest Total Plate Count result was 1,9x106 CFU/g and the lowest was 7,8x104 CFU/g. The result of identification showed that 100% samples examined were contaminated by S. aureus with the highest result was 2,9x104 CFU/g and the lowest result was 4,3x103 CFU/g or exceeding the SNI 7388:2009.
Detection of eae Gene as A Marker Strain EPEC (Enteropathogenic Escherichia coli) in Chicken Meat Sold at Some Traditional Markets in Surabaya Amrullah, Amirul Muslim; Rantam, Fedik Abdul; Raharjo, Dadik; Tyasningsih, Wiwiek; Estoepangestie, Agnes Theresia Soelih; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.31134

Abstract

This study aimed to detect the presence of eae gene in E. coli from chicken meat sold at the traditional market Surabaya. Identification of bacteria was carried out in several stages, inoculated on pre-enrichment media using Buffered Peptone Water (BPW), planting on Levine's Eosin Methylene Blue Agar (L-EMB) media, suspected colonies on L-EMB were subjected to Triple Sugar Iron Agar (TSIA) and Indol test for the confirmation of E. coli. The confirmed E. coli isolates were tested by Polymerase chain reaction (PCR) to determine the presence of the eae gene. The results showed that E. coli could be identified in 28 out of 100 (28%) chicken meat samples, and eae gene was detected in three E. coli isolates (11%).
Assessment of the Rapid Immunochromatographic Test as a Diagnostic Tool for Norovirus Related Diarrhea in Children Ranuh, Reza Gunadi; Athiyyah, Alpha Fardah; PA, Deanty Ayu; Darma, Andy; Raharjo, Dadik; Shirakawa, Toshiro; Sudarmo, Subijanto Marto
Folia Medica Indonesiana Vol. 55 No. 1 (2019): March
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.387 KB) | DOI: 10.20473/fmi.v55i1.24377

Abstract

In developing countries, Norovirus is the second-leading cause of acute diarrhea, after rotavirus. The approved gold standard method for diagnosis of norovirus infection is RT-PCR. The rapid immunochromatographic test is a novel and expedient method for diagnosing norovirus that is relatively affordable. However, the use of the rapid immunochromatographic test remains controversial because of its accuracy. This study aimed to explore whether the rapid immunochromatographic test could be used for diagnosing norovirus-related diarrhea in children. Rapid immunochromatographic test (QuickNaviTM-Norovirus2) and RT-PCR on stool samples was used to diagnose norovirus. Stool samples were obtained from pediatric patients aged between 1 and 60 months who had diarrhea and were admitted to the pediatric ward at Dr. Soetomo General Hospital Surabaya, between April 2013 and March 2014. Ninety-four subjects provided stool samples that were tested using QuickNaviTM-Noro2 and RT-PCR. Using the test, 64 samples tested positive for norovirus and 30 tested negatives. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the rapid immunochromatographic test were consecutively 90.3%, 42.9%, 43.8%, 90%, and 58.5%. RT-PCR was used to test all samples to assess the accuracy, which showed that one from 31 samples contained the GI strain (1.1%), while 30 samples (32%) contained the GII strain. This study definitively establishes that the rapid immunochromatography test is not sufficiently accurate for use as a screening or diagnostic tool in norovirus-related diarrhea cases in children.
THE EFFECT OF BREED AND BODY CONDITION SCORE ON OVARIAN WEIGHT, NUMBER OF FOLLICLES, AND OOCYTE QUALITY IN RETIRED COWS Putri, Natalie Lovena Pramono; Ikhsan, Dilla Amalia Bilqis; Raharjo, Dadik; Khairullah, Aswin Rafif; Lestari, Tita Damayanti; Mustofa, Imam; Rimayanti, Rimayanti; Safitri, Erma; Amrullah, Muhammad Fajar; Ilmi, Laily 'Ulya Nurul; Pasang, Gabriel Sampe; Supriyadi, Supriyadi
Jurnal Kedokteran Hewan Vol 19, No 1 (2025): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i1.44392

Abstract

The aim of this study was to determine whether breed and body condition score (BCS) can influence ovarian weight, follicle number, and oocyte quality. This study used 90 ovaries from 15 Friesian Holstein crossbreed, 15 Ongole crossbreed, and 15 Limousin crossbreed cows obtained from an abattoir. Before the ovaries were taken, the BCS of the cows was determined. The BCS system used in this study is BCS with a scale of 1 to 5. Ovaries were weighed, follicles were measured and grouped by size (2 mm, 2-6 mm, 6 mm) then counted, oocytes were aspirated from the follicles then observed under a microscope. Oocyte quality was observed and then grouped into grade A, B, C, D based on cumulus oocyte complex (COCs). Data were analyzed using two way ANOVA and chi square test. The results of the analysis showed that breed did not influence ovarian weight, but influenced the number of follicles, and oocyte quality. In Friesian Holstein crossbreed cows, BCS affects ovarian weight, follicle number, and oocyte quality. In Ongole crossbreed cows, BCS had no effect on ovary weight, but did affect follicle number and oocyte quality. In Limousin crossbredcows, BCS had no effect on ovary weight and follicle number, but did affect oocyte quality.
IMPROVEMENT DAIRY CATTLE BARN MANAGEMENT AFTER FOOT AND MOUTH DISEASE (FMD) OUTBREAK IN MEDOWO, KEDIRI Permatasari, Dian Ayu; Raharjo, Dadik; Effendi, Mustofa Helmi; Budiarto, Budiarto
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 9 No. 2 (2025): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i2.2025.260-270

Abstract

The Foot and Mouth Disease (FMD) outbreak that began in 2022 caused significant losses for dairy farmers in Medowo Village, Kandangan District, Kediri Regency—impacting both livestock health and farmers' economic stability. To address these impacts, a community service program was carried out to improve farmers' knowledge and skills in dairy cattle housing management and biosecurity implementation. The program included training sessions, educational outreach, and technical assistance for 31 farmers from KUD Kertajaya. Evaluation was conducted through pre-test and post-test instruments to measure the effectiveness of the intervention. Pre-test results showed that only 6.5% of participants were in the "good–very good" category in terms of understanding FMD and livestock management. After the intervention, post-test results showed a significant increase, with 87% of participants reaching the "good–very good" level. Additionally, in terms of practical application of cattle management practices, 100% of participants demonstrated excellent understanding after the program. This intervention proved that an educational approach combining outreach and hands-on practice can significantly enhance farmers' awareness and skills. The improvement is expected to contribute to better livestock productivity and more effective disease control. The activity also involved undergraduate students as part of the Merdeka Belajar Kampus Merdeka (MBKM) program, fostering collaboration between academia and the farming community to strengthen local capacity.
Gecko (Gekko gecko) Captive Breeding Development Strategy In East Java Bugiono, Bugiono; Raharjo, Dadik; Hidanah, Sri; Soeharsono, Soeharsono; Ardianto, Ardianto; Setyawan, Boedi
El-Hayah:Jurnal Biologi Vol 10, No 1 (2024): EL-HAYAH (VOL 10, NO 1 September 2024)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v10i1.31888

Abstract

Geckos (Gekko gecko) are widely used in traditional Chinese medicine and are reported to have anti-tumor properties. Approximately 93% of reptiles exported from Indonesia come from the wild. The direct and continuous capture of geckos in nature will threaten gecko populations in the future. As an effort to control the possible threat of extinction, the Indonesian government encourages the utilization of this animal through captive breeding activities. The existence of gecko (Gekko gecko) captive breeding units in East Java has not been widely developed and still uses conventional and semi-natural patterns. Periodic evaluations need to be carried out to determine whether or not this breeding activity is feasible and what the development strategy is in the future. This study aims to analyze the strengths, weaknesses, opportunities and threats in order to formulate a business development strategy for gecko breeding in East Java. This research uses a survey method by analyzing and describing the problems in the field, while the technical analysis uses internal factor analysis (IFE matrix), external factor analysis (IFE matrix) and SWOT analysis. Based on the results of the SWOT analysis, the development strategy of the gecko (Gekko gecko) breeding business in East Java includes: maintaining market confidence and maintaining quality and production stability; adjusting government regulations related to gecko trade governance; developing effective breeding methods; adjusting government regulations related to breeding governance; establishing market strategies and production stability to suppress illegal trade; and researching new market potential for gecko trade development
Description of antemortem inspection of cattle at the Dimoro slaughterhouse, Blitar City Hasibuan, Sandi Aziz; Lokapirnasari, Widya Paramita; Triakoso, Nusdianto; Budiarto, Budiarto; Warsito, Sunaryo Hadi; Raharjo, Dadik
Jurnal Agro Veteriner Vol. 9 No. 1 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v9i1.78414

Abstract

The outbreaks of Foot and Mouth Disease (FMD) and Lumpy Skin Disease (LSD) in early 2024 significantly impacted livestock health conditions in Blitar City and led to an increase in emergency slaughter cases. The Dimoro Slaughterhouse plays a crucial role in ensuring the safety of animal-derived food through the implementation of antemortem inspections. This study aims to evaluate the health status of beef cattle and identify pathological conditions detected during antemortem inspections at the Dimoro Slaughterhouse. The research used a quantitative descriptive method with direct observation of 40 cattle during the period of June 13–19, 2025. Clinical examinations were conducted on the head, neck, thorax, abdomen, anus, and limbs. The results showed that most cattle were male (34 heads) and predominantly of the Limousin breed (19 heads). No clinical symptoms of FMD, LSD, fever, or other disorders warranting slaughter rejection were found. Mild findings such as dirt around the anus were recorded in some individuals. Overall, the antemortem inspection was carried out effectively and contributed to ensuring the safety of meat products for public consumption.
Co-Authors Alpha Fardah Athiyyah Amrullah, Amirul Muslim Amrullah, Muhammad Fajar Andy Darma Budiarto Budiarto Bugiono, Bugiono Caska - Didik Handijatno Erma Safitri Estoepangestie, Agnes Theresia Soelih Fedik Abdul Rantam Harijani, Nenny Hasibuan, Sandi Aziz Hidanah, Sri I Gusti Made Reza Gunadi Ranuh Ikhsan, Dilla Amalia Bilqis Ilmi, Laily 'Ulya Nurul IMAM MUSTOFA Khairullah, Aswin Rafif Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Ni Made Mertaniasih Nusdianto Triakoso PA, Deanty Ayu Pasang, Gabriel Sampe Permatasari, Dian Ayu Putri, Natalie Lovena Pramono Rimayanti Rimayanti Sakran, khawla Abdullah Setyawan, Boedi Shirakawa, Toshiro Soeharsono Soeharsono Sudarmo, Subijanto Marto Supriyadi Supriyadi Tacharina, Martia Rani Tita Damayanti Lestari Tyasningsih, Wiwiek Warsito, Sunaryo Hadi Widya Paramita Lokapirnasari