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Journal of Bio-molecule Research And Engineering
Published by Universitas Airlangga
ISSN : -     EISSN : 2962343X     DOI : https://doi.org/10.20473/jbiome.v1i2.45225
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology
Journal of Bio-Molecule Research and Engineering [JBIOME] is a peer-reviewed, open-access journal that publishes original research articles, review articles, as well as short communication in the following areas: Biochemistry, Biotechnology, Bioengineering, Microbiology, Molecular Biology, Synthetic Biology, Natural Product Exploration and Extraction, Organic synthesis, Drug discovery, Bioinformatics, Interdisciplinary approaches to Biomolecule Engineering.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 19 Documents
 1   2 
Identification of Saprophytic Mold Isolated from Mangrove Soil: A Review Rahmawati, Hanif Nur; salsabila, Syarifah salsabila; Rahayuningtras, Nazil Dwi; Nurhariyati, Tri; Fatimah
Journal of Bio-Molecule Research and Engineering Vol 2 No 1 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i1.50546

Abstract

Saprophytic mold is one type of mold that has the ability to survive in extreme conditions, such as in mangrove soil. Saprophytic fungi act as the main organism in the process of decomposition of organic matter and energy flow in the mangrove area. Several researchers successfully identified 102 species and 33 genera of saprophytic fungi. Aspergillus is known to be the dominant mold on mangrove soils because of its characteristics, such as high sporulation and spore spread and its ability to withstand extreme environmental conditions. The use of saprophytic molds in biofertilizer manufacturing due to saprophytic molds can produce cellulase, xylinase, and ligninase enzymes to decompose organic matter and produce toxin compounds for pathogens in plants. The presence of mold species in mangrove soil is influenced by several factors, including the temperature level, season, the salinity of mangrove waters, soil pH, and oxygen.
Evaluation on Bioactive Secondary Metabolites from Fingerroot (Boesenbergia rotunda) as a Potent α-Glucosidase Inhibitor: In silico Study: In silico Study Firdaus, Yazdaniar Fajri Halimi; Hariyanto, Nova Alfian; Kurnia, Irmayanti Tri; Lestari, Dinda Ayu; Suwandari, Suhartini; Haq, Kautsar Ul; Phuwapraisirisan, Preecha; Ramadhan, Rico
Journal of Bio-Molecule Research and Engineering Vol 2 No 1 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i1.51981

Abstract

Fingerroot (Boesenbergia rotunda) is a medicinal plant that has been reported to have anti-diabetic properties. However, the mechanism of action and the active compounds responsible for this effect are not well understood. In this study, we performed molecular docking study of isolated compound from fingerroot against N-terminal-human intestinal maltase-glucoamylase, one class of α-glucosidase. Inhibiting enzymatic activity of alpha glucosidase could potentially control sugar levels. In addition to panduratin A showing moderate inhibition activity against N-terminal-human intestinal maltase-glucoamylase.
Potential of Microbial Isolates from Indonesia in Producing Antimicrobial Compounds: A Review Geraldi, Almando; Wijaya, Nabilla Hapsari; Clement, Christopher; Hajar, Versa Rachmania
Journal of Bio-Molecule Research and Engineering Vol 2 No 1 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i1.52001

Abstract

Indonesia, as a megabiodiverse country, possesses a wealth of beneficial microorganisms, including bacteria and fungi capable of producing antimicrobial compounds. Over the past decade, extensive research has been conducted in Indonesia to explore and screen the antimicrobial activities of bacteria and fungi. These microorganisms have been isolated from various ecosystems, such as terrestrial, maritime, extreme environments like hot springs and sand dunes, as well as from animals and plants hosts. Most studies have focused on the antimicrobial activity of crude metabolite extracts, which demonstrate inhibitory effects against clinically significant pathogens, including Methicillin-resistant Staphylococcus aureus, multidrug-resistant Escherichia coli, and Salmonella typhi. This review systematically synthesizes findings from peer-reviewed literature, detailing isolation strategies, antimicrobial screening techniques, and the bioactivities reported. By consolidating current knowledge, it aims to elucidate the potential of Indonesia’s microbial resources for developing novel antimicrobial agents and promoting their sustainable exploitation within the context of global health challenges.
Evaluation potency of Boesenbergia rotunda as antioxidant achieved by free radicals scavenging activities Dinda; Kurnia, Irmayanti Tri; Hariyanto, Nova Alfian; Suwandari, Suhartini; Firdaus, Yazdaniar Fajri Halimi; Mahmuda, Nur Fariha; Phuwapraisirisan, Preecha; Ramadhan, Rico
Journal of Bio-Molecule Research and Engineering Vol 2 No 1 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i1.52066

Abstract

Boesenbergia rotunda has antioxidant potential to inhibit free radicals. Antioxidant activity was determined by free radical scavenging activity against DPPH, ABTS and Cuprac. The results showed that the hexane extract of Boesenbergia rotunda had a strong percent antioxidant activity with a value of 91.38, while ascorbic acid as a positive control had a value of 99.85. Antioxidant activity test results showed that hexane and dichloromethane extracts have DPPH, ABTS, and cuprac free radical scavenging activities
The Analog Rice from Gembili Tubers which Contain Various of Benefits: An overview Iriana, Tumiar; kusumayanti, Heny
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.45653

Abstract

Gembili (Dioscorea esculenta), has very good potential, one of which is that the tubers produced have many benefits, apart from being believed to contain high carbohydrates, gembili also contain calcium, potassium, iron and vitamin B6. Apart from being used as a simple food, gembili tubers can be used as an analogue of rice. This review aims to examine and find out the various benefits of gembili tubers, the combination of various agricultural ingredients in producing analog rice and the resulting characteristics. The method of making analog rice is done by extruder. Test how many studies include proximate tests, antioxidants, calcium, levels of fiber and amino acids. Based on several studies of analog rice that made from gembili with tubers 78%, protein content 8%, water content 7.9%, ash content 2-3.5%, fat content 0.5-1.2%, antioxidants 6162 ppm, fiber crude 10% and calcium 1.52%, each. The characteristics of rice is shape, texture, color, and taste to can be accepted by the community. Analog rice can be used as a substitute for rice.
Assessment of the Phytochemicals and Antioxidant Activity of Baccaurea bracteata Müll.Arg. and Macaranga lowii King ex Hook.f. from East Kalimantan Dinda; Suwandari, Suhartini; Kurnia, Irmayanti Tri; Hasan, Muhammad Iqbal; Artama, Riro Iraga; Phontree, Kiettipum; Ramadhan, Rico
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.65361

Abstract

This study investigated the total phenolic content and antioxidant activity of Baccaurea bracteata Müll.Arg. and Macaranga lowii King ex Hook.f. from East Kalimantan. The leaves of these plant extracts were evaluated for their total phenolic content (TPC) and antioxidant activity, utilizing the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. The findings indicated that Baccaurea bracteata Müll.Arg. demonstrated superior antioxidant activity with a percent inhibition (PI) value of 68.31 ± 0.11%. This research highlights Baccaurea bracteata Müll.Arg. as a promising source of natural antioxidants, characterized by high total phenolic content of 14.76 ± 1.98 mgGAE/g.
Studies of Yeasts Isolated from Soil as Cellulose Decomposers and Phosphate Solvents Putra, Gading Gunawan; salsabila, Syarifah salsabila; Rahmawati, Hanif Nur; fatimah
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.65587

Abstract

The identification of CMCase (Carboxymethylcellulase) and PMEase (Phosphomonoesterase) enzyme activities from yeast genera that produce cellulase and phosphatase is crucial for identifying potential genera that could aid in the development of biofertilizers, serving as an environmentally friendly alternative to chemical fertilizers. This study is based on a review of articles and journals for data collection. The review revealed that the yeast genus Rhodosporidium (specifically Rhodosporidium paludigenum Y08RA29) is a promising cellulolytic yeast, with CMCase activity approaching 0.500 units. Meanwhile, the potential phosphate-solubilizing yeast genus is Candida (Candida sp. 3), with PMEase activity ranging from 0.05 to 0.06 units.
Hydrolytic Enzyme Producing Bacteria From The Gut Of Oryctes rhinoceros Larvae Inhabiting Household Waste Composer Ana Mariatul Khiftiyah; Ni'matuzahroh; Mochammad Affandi; Salamun; Silvia Kurnia Sari; Miftahul Jannah; Nastiti Trikurniadewi; Achmad Zainal Abidin; Nurul Wahyuni; Rizki Amaliah Zain; Dela Dwi Alawiyah; Brigita Nur Diyan Agustiana
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.67564

Abstract

relatively shorter adult phase than the larval stage. The larval stage of this organism lives in piles of organic matter, including in piles of household waste. The ability of larvae to utilize organic matter in their environment is inseparable from the role of potential microbes that produce enzymes associated with their gut. This study aims to isolate, screen, and identify the potential bacteria in producing hydrolytic enzymes from gut of O. rhinoceros larvae. The third instar larvae were dissected aseptically to obtain gut bacterial isolates. The isolates were tested for their ability to produce amylase, cellulose, lipase, and protease using agar plate containing specific substrates. There were eight different isolates, and three of them had the ability to produce all four enzymes. The three isolates were identified as Bacillus. These results can be used as a reference for handling household organic waste.
Pycnoscelus surinamensis Endosymbiont Bacteria: Identification and its Amylase Characteristic Ni'matuzahroh; Fatimah; Sholikah; Ayu Tri Wahyuni; Tri Nurhariyati; Mochammad Affandi; Agus Supriyanto; Almando Geraldi; Tini Surtiningsih; Brigita Nur Diyan Agustiana; Dela Dwi Alawiyah; Ana Mariatul Khiftiyah; Silvia Kurnia Sari
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.67571

Abstract

The amylase is an enzyme that widely used in industry. In this research, amylase was isolated from endosymbiont bacteria (EKA 4) of cockroach Pycnoscelus surinamensis from household garbage dump Surabaya. This research aims to identify bacteria EKA 4, produce and characterize amylase from it at various temperature, pH and effect of metal ions. Amylase screening assay was done by imoculating the isolate into medium contain 2% of starch then incubated for 24 hours. Detection of amylase was done qualitatively by measured presence of halozone and quantitatively using DNS method. 16S rRNA analysis found that bacteria EKA 4 has similarity to Bacillus subtilis with 100% of percent identity. Result showed that optimum activity of amylase from B. subtilis were at 40°C, pH 6 and could use ion CaCl2 as a cofactor in a reaction. Thus, utilization of amylase from endosymbiont P. surinamensis could be as an alternative source in big industries.

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