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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general.
Arjuna Subject : Umum - Umum
Articles 21 Documents
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Search results for , issue "Vol. 12 No. 1 (2025)" : 21 Documents clear
THE ANTIMYCOBACTERIAL POTENTIAL OF SAMBILOTO (Andrographis paniculata Nees) EXTRACT AGAINST Mycobacterium tuberculosis H37Rv WITH Microscopic-Observation AND Drug-Susceptibility (MODS) METHODE Asarini; Abdillah, Syamsudin; Sani, Yulvian; Alam, Gemini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.8626

Abstract

Andrographis paniculata, commonly known as sambiloto, is empirically used for various treatments, including its ability to inhibit the growth of Mycobacterium tuberculosis (M. tuberculosis). This study aims to evaluate the growth inhibition activity of M. tuberculosis strain H37Rv using sambiloto herb extract (Andrographis paniculata). The sambiloto extract was prepared using the maceration method. M. tuberculosis was grown on MODS medium, and the toxicity of sambiloto (A. paniculata) was analyzed using pharmacokinetic prediction studies (pkCMS). The results showed that at concentrations of 0.255 mg/ml, 1.275 mg/ml, and 2.55 mg/ml of sambiloto extract, M. tuberculosis growth occurred on days 7 to 14. Further observations were made until day 28, and it was found that starting at a concentration of 6.375 mg/ml, sambiloto extract did not show M. tuberculosis growth in MODS medium. The positive control, isoniazid, did not show bacterial growth, while the negative control showed extensive bacterial growth on day 12. The determination of M. tuberculosis growth was based on microscopic observations of the bacterial colonies, focusing on cord formation. In conclusion, this study, the use of an extract with a concentration of 6.375 mg/ml in the MODS method showed no growth of Mycobacterium tuberculosis, indicating that the extract is effective in inhibiting bacterial growth at this concentration. The online pkCSM test conducted in this study showed that the extract used is not cytotoxic, meaning that the extract is safe for body cells and does not cause cellular damage. Therefore, it has the potential to be an adjunct therapy in the treatment of tuberculosis.
THE EFFECT OF AMMONIUM-BASED NPK FERTILIZERS ON THE GROWTH OF MANGROVE SEEDLINGS Rhizopora mucronata AND Rhizopora stylosa Hanindipto, Fasa Aditya; Rasima Daulay, Fathin; Puji Rahayu, Sarwendah; Putri Irsani, Izma; Rizki Rachmani, Alfina; Evangelina, Helga
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.8938

Abstract

Mangroves play an important role in coastal ecosystems, but their growth is often limited by nutrient availability, particularly nitrogen, phosphorus, and potassium (NPK) availability. The use of ammonium-based fertilizers can provide additional benefits by accelerating the growth of mangrove seedlings. This study examines the influence of various doses of ammonium-based NPK fertilizers on the growth of two mangrove species, Rhizophora mucronata and Rhizophora stylosa, The study was carried out in two different places with distinct environmental characteristics, Rhizophora mucronata was planted near land, whereas Rhizophora stylosa was planted in open areas near the sea. The seedlings received dosages of ammonium-based NPK fertilizer. Periodic measurements of growth characteristics, including leaf length, root length, plant height, and SPAD (Soil Plant Analysis Development) index, were conducted and tested statistically. Rhizophora mucronata responded effectively to ammonium-based fertilizer treatment, increasing plant height, root length, and chlorophyll content at an optimal dose of 0.9 grams per, although shoot growth decreased. These data show that Rhizophora mucronata is more responsive to ammonium- based fertilizer treatments than is Rhizophora stylosa under the evaluated environmental conditions, although the fertilizer dosage had no significant effect.
EFFECTIVENESS OF VARIOUS COMMERCIAL EXTENDERS IN CRYOPRESERVING SEMEN OF LOCAL INDONESIAN RAMS Setiadi, Dedi Rahmat; Magfira; Riwu, Ronny Markus Jami; Arifiantini, R Iis
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9208

Abstract

Indonesian local sheep represent a particular species of sheep reared by farmers on small-scale farms. Semen freezing can be performed using either a homemade or commercial extender. The present study evaluated the effectiveness of several commercial extenders in freezing Indonesian local sheep. Semen was collected from three mature and healthy rams using an artificial vagina and evaluated. It was determined that only samples exhibiting sperm motility levels of greater than 70% would be included in the study. Subsequently, the semen from each ram was divided into three portions and diluted with Andromed, Sterydil, and OptiXcell, respectively. The frozen semen was then evaluated 24 hours post-freezing. The thawing process was conducted individually at 37°C for 30 seconds. The evaluation encompassed a range of metrics, including sperm motility, viability, and the presence of any abnormalities, in addition to the recovery rate. The results indicated that sperm frozen in Optixcell and Sterydil extenders exhibited superior motility, viability, and recovery rate than Andromed. The study also demonstrated no interaction between individual Ram and extender, and no differences were found between individuals. The study concluded that Optixcell and Sterydil extenders effectively froze Indonesian local sheep semen.
EXTRACT AND FRACTIONS EXPLORATION OF DAYAK ONION (Eleutherine America-na Merr.) WITH ANTIOXIDANTS POTENTIAL USING GC-MS and FTIR METHODS Elisa, Novi; Advistasari, Yustisia Dian; Lepangkari, Jaka Seprianto; Hidayatullah, Muhammad Haqqi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9209

Abstract

Free radicals can cause various diseases in the body if not treated immediately. Large levels of free radicals may trigger damage to normally functioning tissues, lipid layers, blood vessels, DNA synthesis disturbances, and even destruction of cells. The objective is to evaluate the antioxidants potential of dayak onion extract and fractions. FTIR spectrophotometers, gas chromatography-mass spectrometry (GC-MS) and an antioxidants multimode reader are some of the research methods. The outcomes of this research are identifying plants that showed the dayak onion extract of the species Eleutherine Americana Merr. It contains compounds such as flavonoids, alkaloids, tannins, and saponins. In addition, the components found in the extract, water fraction, ethyl acetate fraction, and n-hexane fraction are octadecanoic acid 298 m/z, 9-octadecanoic acid 282 m/z, undecanoic acid 214 m/z, hexadecanoic acid 368 m/z, and nonadecanoic acid 326 m/z. Therefore, the antioxidants test using the DPPH method showed that the best IC50 value is for ethyl acetate with IC50 20.56 ppm.
THE POTENCY OF PSIDIUM GUAJAVA LEAVES AS A NATURAL DISINFECTANT AND ANTISEPTIC INGREDIENT Mahyuni, Siti; Komala, Oom; Wiendarlina, Ike Yulia; Fatah, Muhammad Fajar Saeful
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9216

Abstract

Preventing the spread of nosocomial infection are generally carried out by disinfection process of the tools to be used with chemical disinfectant such as phenol, alcohol, chlorine, iodine or sublimate. Excessive use of chemicals can cause health problems. Guava leaves (Psidium guajava L.) have long been empirically used to treat diarrhea and acne. Scientific data shows that guava leaves have positive activity against several types of pathogenic bacteria due to its tannin content. Guava leaves have the potential to be developed as a disinfectant and antiseptic material. This study aims to determine the disinfection activity of guava leaves on surgical instruments using the total plate count method, skin irritation test using patch test method and quantify the tannin content in young and old guava leaves using spectrophotometry method. The results showed that the tannin content in old and young guava leaves was 68.73 ± 0.067 mg/g (6.873%) and young 61.87 ± 0.046 mg/g (6.187%). At concentrations of 10, 20, 30 and 40% guava leaf extract were able to reduce the total plate count in surgical instrument samples by 6.85; 37; 80.82; and 83.56%. The primary irritation index of 0.05 only occurs at a concentration of 40%, classified as non-irritant. It can be concluded that guava leaves have the potential to be further developed as a natural disinfectant or antiseptic.
OPTIMIZING VIRGIN COCONUT OIL YIELD AND QUALITY USING LACTIC ACID BACTERIA FROM BLONDO WITH CHILLING-THAWING METHOD Chaidir, Riri Rimbun Anggih; Jariah, Ainun; Nevara, Gita Addelia; Murti, Tri; Rohayu, Andini Cindy
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9246

Abstract

Increasing demand for Virgin Coconut Oil (VCO) presents a promising opportunity to improve the economy in Indonesia. Various methods for VCO production have been explored to optimize yield and ensure desirable organoleptic and physicochemical qualities. Modifying the oil extraction process often necessitates costly equipment. In this study, VCO was extracted by combining the chilling-thawing method with the addition of lactic acid bacteria (LAB) isolated from blondo. The process began with the isolation and characterization of LAB isolates, followed by the application of the chilling-thawing method and addition of 2% LAB starter, then assessed for their consumer preference through a questionnaire. This innovative process yielded VCO at a rate of 35.78-46.65% using three LAB isolates (BAL 1-3) from blondo samples, representing an improvement over previous studies employing similar methodologies. Moreover, hedonic evaluation revealed a higher consumer preference for LAB-treated VCO compared to the control, further highlighting the effectiveness of LAB treatment in combination with the chilling-thawing method.
EFFECT OF GROWING MEDIA ON THE GROWTH OF Streptomyces sp. AND Trichoderma harzianum AS ANTHRACNOSE DISEASE BIOCONTROL Rulinggar, Nia; Mujoko, Tri; Wuryandari, Yenny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9257

Abstract

Streptomyces sp. and Trichoderma harzianum is a biological agent that is effective in controlling Colletotrichum capsici, the cause of disease in chili plants. Different growing media can affect the growth and activity of these two microorganisms. This study aims to evaluate the growth of Streptomyces sp. and Trichoderma harzianum on various media, namely rice washing water, coconut water, and sugar potato extract. The research phase includes growing media test, effectiveness test of Streptomyces sp. and Trichoderma harzianum in chili peppers, and effectiveness test of Streptomyces sp. and Trichoderma harzianum on chili seeds. The results showed that the medium of rice washing water is more suitable for Streptomyces sp., coconut water media is more suitable for Trichoderma harzianum, and rice washing water media is more suitable for the combination of the two biological agents. Optimal growth in these media affect the effectiveness of biocontrol in suppressing the severity of anthracnose disease in chili.
PHARMACOLOGICAL INVESTIGATION OF THE ORAL WOUND HEALING ACTIVITY OF Stachytarpheta jamaicensis ROOT EXTRACT GEL IN WISTAR RATS Utami, Juliyatin Putri; Wasiaturrahmah, Yusrinie; Carabelly, Amy Nindia; Aspriyanto, Didit; Nahzi, Yanuar Ichrom; Arifin, Rahmad; Ramadhaniyah, Nur; Jatmiko, Habibi Naufal
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9390

Abstract

The Stachytarpheta jamaicensis (L) Vahl root contains bioactive compounds like saponins, alkaloids, flavonoids, tannins, phenols, and terpenoids, which have antibacterial and anti-inflammatory properties. This study investigates the wound healing effects of Stachytarpheta jamaicensis (L) Vahl root extract gel in oral mucosa. The experiment involved 28 male Wistar rats, divided into four groups: a positive control treated with povidone iodine 10%, a negative control treated with gel base, and two experimental groups treated with 6% and 10% S. jamaicensis root extract gels. The gel was applied twice daily, and wound healing was assessed by epithelial thickness, inflammation cell and hydroxyproline content. Both concentrations of S. jamaicensis root extract gel significantly enhanced wound healing, as shown by increased epithelial thickness, inflammation cell and hydroxyproline content levels compared to the negative control. These findings suggest that S. jamaicensis root extract gel effectively promotes wound healing in the excision wound model.
THE COMPARISON BETWEEN LOGISTIC REGRESSION AND CONVOLUTIONAL NEURAL NETWORK FOR MULTI-DRUG RESISTANT TUBERCULOSIS PREDICTION Widjaja, Albert; Wibowo, Satrio; Parikesit, Arli Aditya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9769

Abstract

Multi-drug resistant tuberculosis (MDR-TB) is caused by Mycobacterium tuberculosis strains that resist at least two first-line anti-TB drugs. This disease presents a major global health challenge, particularly affecting middle to lower income countries where affordable and rapid diagnostic tools are urgently needed. To address this, researchers are exploring the combination of whole genome sequencing and machine learning for drug resistance predictions. Using Mycobacterium tuberculosis genomic data from databases, both Logistic Regression (LR) and Convolutional Neural Network (CNN) models were trained to predict drug resistance. Performance evaluation revealed that CNN slightly outperformed LR in accuracy and specificity for Rifampicin and Pyrazinamide predictions, while LR showed better results for Isoniazid and Ethambutol. In terms of sensitivity, LR demonstrated superior performance for most drugs, except Ethambutol where CNN excelled. Though computational complexity assessment was incomplete due to hardware limitations, both models showed distinct advantages in predicting first-line anti-TB drug resistance.
FROZEN SEMEN CHARACTERISTICS OF BALI BULL IN DIFFERENT AGE GROUPS Witri, Brilla Widya; Arifiantini, Iis; Prasetyaningtyas, Wahono Esthi; Karja, Ni Wayan Kurniani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10087

Abstract

One factor that affects frozen semen characteristics is bull age. Increasing age induces changes in sperm that reduce frozen semen quality. This study aimed to evaluate the frozen semen characteristics of Bali bulls aged 3, 8, and 13 years. Frozen semen was derived from fresh semen with sperm motility > 70%. The frozen semen characteristics tested included motility, viability, plasma membrane integrity, acrosome integrity, and sperm abnormalities. Sperm motility was analyzed by computer-assisted sperm analysis (CASA). Viability was assessed using eosin-nigrosin staining; plasma membrane integrity was assessed using the hypoosmotic swelling test (HOST); acrosome integrity was assessed using Giemsa staining; and sperm abnormalities were assessed using Williams staining. The plasma membrane integrity of the 13-year-old group was significantly lower (P < 0.05) than that of the younger age groups. However, there were no significant differences (P > 0.05) in the other parameters. We conclude that age affects the integrity of the sperm plasma membrane in frozen semen from Bali bulls.

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