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Lussana Rossita Dewi
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Bioma : Jurnal Ilmiah Biologi
Published by Universitas PGRI Semarang
ISSN : 20865481     EISSN : 25499890     DOI : https://doi.org/10.26877
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology
Jurnal Ilmiah Biologi focuses on the publication of result’s research related with the major of biology and biology education. For the major of biology, including systematics, physiology and metabolism, ecology, bioconservation, cell and developmental biology, microbiology, genetics, molecular biology, biotechnology, and bioinformatics. For the major of biology education, including biology curriculum, teaching biology, instructional media, and assessment.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 80 Documents
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CRI PROFILE: CLIMATE CHANGE LITERACY AND SUSTAINABILITY AWARENESS Agus Purnomo; Muhammad Syaipul Hayat; Joko Siswanto; Siti Patonah
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 2 (2026): October 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i2.4232

Abstract

This study aimed to analyze the profile of Climate Change Literacy (CCL) and Sustainability Awareness (SA) among junior high school students on the topic of global warming using the Certainty of Response Index (CRI) instrument and to map understanding patterns based on students' academic categories. A descriptive quantitative design was employed involving 14 Class IX students at SMPN Satu Atap 5 Hanau, Seruyan Regency, Central Kalimantan. Data were collected using a 20-item multiple-choice test integrated with a 0–5 CRI scale, then categorized into four understanding types: Conceptual Understanding, Lucky Guess, Misconception, and Does Not Know the Concept. Results showed that 35.7% of students demonstrated good conceptual understanding, 42.9% were classified as Sufficient Understanding, 14.3% showed dominant misconceptions, and 7.1% did not understand the concepts; overall, 23.9% of all responses were identified as misconceptions. The most common misconceptions were on greenhouse gas sources from the agricultural sector (item 6: 6 students) and mitigation-adaptation terminology (item 17: 5 students). Academic category analysis showed that high-achieving students were predominantly in the understanding category, while low-achieving students showed patterns of misconception and incomplete understanding.
DIVERSITY AND ETHNOBOTANICAL USES OF MEDICINAL ZINGIBERACEAE Ayu Anggraini; M. Anas Dzakiy; Eko Retno Mulyaningrum
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 2 (2026): October 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i2.4220

Abstract

This study aims to document medicinal Zingiberaceae taxa and examine patterns of plant-part use, preparation, administration, and acquisition in Tambakromo, Pati, Central Java. A descriptive qualitative study was conducted from February to March 2025 in Karangmulyo, Keben, Sinomwidodo, and Angkatan Kidul Villages. Data were collected through structured face-to-face interviews with 13 community informants, field observations, documentation, and plant identification using botanical literature. The informants comprised 10 women and 3 men aged 41–84 years. Twelve Zingiberaceae taxa were documented. Field surveys recorded the highest number of individual plants for ginger (Zingiber officinale Roscoe) (n = 34), followed by Javanese turmeric (Curcuma zanthorrhiza Roxb.) (n = 30), turmeric (Curcuma longa L.) (n = 27), and galangal (Alpinia galanga (L.) Willd.) (n = 26). Informant citation analysis showed that turmeric had the highest Frequency of Citation (FC = 9; RFC = 0.692), followed by Javanese turmeric and aromatic ginger (FC = 7; RFC = 0.538 each). Rhizomes dominated plant-part records (87.9%), boiling was the most common preparation method (54.5%), and drinking was the predominant route of administration (72.7%). Home cultivation was the main source of acquisition (49%).
MAKING LIQUID ORGANIC FERTILIZER FROM BLACK SOLDIER FLY LARVA LEACHATE USING A VARIETY OF CANTEEN WASTE Adrianto Prihartantyo; Hesekiel Mitra Insan; Nina Roberta Turnip; Astiti Aditia
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 2 (2026): October 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i2.3739

Abstract

This research aims to develop a POC using BSF larvae leachate and bioactivators as a preliminary study for producing POC using a fermenter in the form of a biodigester. The POC production process involves inserting BSF waste larvae (kasgot) and water into the biodigester at a 1:1 ratio and adding the EM-4 bioactivator; in other variations, only local microorganisms found in kasgot are used. BSF larvae were fed in two variations: vegetable waste and IT Del canteen mixed waste. POC was produced in a biodigester for 28 days. This research analysed pH, temperature, and NPK levels. The results show that POC meeting SNI quality standards was obtained from vegetable waste samples, with a final pH of 5.02 and N content of 0.2315%, P of 0.4031%, and K of 0.4929%. The bioactivator used during fermentation of BSF larval leachate influenced the quality of the resulting liquid organic fertiliser. The EM-4 + molasses + leachate treatment yielded the best quality, achieving the highest total NPK content (0.66%) after 15 days of fermentation. The best treatment for plant growth was the liquid organic fertiliser produced after 12 days of EM-4 fermentation, which produced an average plant height of 11.9 cm.
VEGETATIVE MACROMORPHOLOGICAL CHARACTERIZATION OF SIX  Syzygium spp. SPECIES Dhiya Aisyah; Putri Endah Pratiwi; Wira Yuda Ihza Kusuma; Reva Fransisca; Amanda; Fadila Suparti; Weni Lestari; Kurratul 'Aini
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 2 (2026): October 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i2.4100

Abstract

This study aimed to investigate the external morphological characteristics of the roots, stems, and leaves of six Syzygium species through direct visual observations in South Sumatra, Indonesia. The study was conducted in May 2026 across various residential areas and natural habitats using a qualitative descriptive approach. Fresh specimens of Syzygium jambos (L.) Alston, Syzygium cumini (L.) Skeels, Syzygium malaccense (L.) Merr. & L.M.Perry, Syzygium aqueum (Burm.f.) Alston, Syzygium polyanthum (Wight) Walp., and Syzygium aromaticum (L.) Merr. & L.M.Perry were documented and measured directly in their natural habitats. The results showed that all six species shared several general morphological characteristics, including a taproot system; woody, cylindrical, and erect stems; and green, petiolate, simple leaves with entire margins, pinnate venation, opposite phyllotaxis, and smooth surfaces. Despite these shared vegetative characteristics, the species can be distinguished by diagnostic morphological variations, particularly stem surface characteristics and branching patterns, as well as leaf length-to-width ratios, shape, apex, and base. Distinctive macromorphological variations in stems and leaves can serve as preliminary diagnostic characters for distinguishing closely related species, particularly when reproductive organs are unavailable.
THE POTENTIAL OF STEAM-BASED SCIENCE LEARNING WITHIN A DEEP LEARNING FRAMEWORK FOR THE TOPIC OF ENVIRONMENTAL POLLUTION Wartoyo Wartoyo; FENNY ROSHAYANTI; MUHAMMAD SYAIPUL HAYAT
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 2 (2026): October 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i2.4170

Abstract

This study aimed to analyze the potential for implementing an integrated STEAM–Deep Learning model on the topic of Environmental Pollution at the junior secondary school level, prior to full-scale implementation. A descriptive quantitative survey approach was employed, involving science teachers and a Grade IX class as research subjects. Readiness was measured across four variables—Learning Tools, Supporting Facilities, Human Resources (Teachers), and Student Habits—using an expert-validated Likert-scale questionnaire, and data were analyzed using descriptive mean scores converted into qualitative categories. The results showed that Supporting Facilities obtained the highest mean score of 3.25 (fairly adequate), Learning Tools scored 2.76 (fairly good/valid), Human Resources (Teachers) scored 2.72 (moderate), and Student Habits obtained the lowest score of 2.24 (rare). Overall, the potential for implementing STEAM–Deep Learning on the topic of environmental pollution is fairly significant, but two main challenges remain: strengthening teachers' pedagogical competence in integrating the Engineering and Art dimensions, and shifting students' learning habits from surface, teacher-directed routines toward mindful, meaningful, and joyful project-based inquiry. These findings imply that successful adoption of STEAM within the Deep Learning framework depends less on physical infrastructure, which is already comparatively ready, than on sustained teacher professional development and the deliberate cultivation of independent, project-oriented learning habits among students.
ANTIBACTERIAL ACTIVITY OF A HAND SANITIZER BASED ON ASIAN SWEET FLAG (Acorus calamus L.) AND FRAGRANT PANDAN (Pandanus amaryllifolius Roxb. ex Lindl.) LEAVES EXTRACTS AND GC-MS PROFILING OF BIOACTIVE COMPOUNDS Uun Rohmawati; Shaddiqah Munawarah Fauziah; Luklu'ul Karimah
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 2 (2026): October 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i2.3575

Abstract

This research aimed to evaluate the antibacterial effectiveness of a herbal hand sanitizer from a combination of methanol extracts of Asian Sweet flag (Acorus calamus L.) and fragrant pandan (Pandanus amaryllifolius Roxb. ex Lindl.) leaves, and to identify the bioactive compounds by GC-MS analysis. Asian sweet flag (Dlingo in Javanese)  and pandan leaves were macerated in methanol. The extracts were subjected to phytochemical screening and formulated into hand sanitizer preparations with concentrations of 25%, 50%, 75%, and 100%, including a single 100% formulation of each extract. The disc diffusion method was used to investigate antibacterial activity against Staphylococcus aureus and Escherichia coli, while bioactive compounds were identified by GC-MS. The results showed that the extraction yielded 17.75% for dlingo leaves and 18% for pandan leaves. Phytochemical analysis identified flavonoids, tannins, alkaloids, and saponins in both extracts. All formulations inhibited S. aureus and E. coli, with the highest activity in the 100% combination formulation, producing inhibition zones of 9.60 mm and 6.47 mm. GC-MS analysis identified 196 compounds dominated by phenolics, aromatic aldehydes, benzofurans, and long-chain fatty acids with antibacterial potential. The study concludes that the combination of dlingo and pandan leaf extracts has potential as a natural active ingredient in herbal hand sanitizer formulations.
EFFECT OF DRYING TEMPERATURE ON ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC CONTENT, AND TOTAL FLAVONOID CONTENT OF Chrysanthemum morifolium L. Flowers Ambar Pratiwi; Lulu Fitriani; Novi Febrianti; Eri Eryati
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 2 (2026): October 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i2.3672

Abstract

This study explores the effects of drying temperatures (40, 50, and 60°C) on the extraction yield, antioxidant potential, total phenolic content, and total flavonoid content of Chrysanthemum flower simplicia. Four sample groups were evaluated: fresh flowers and flowers dried at 40, 50, or 60°C. The antioxidant activity was analysed by the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, with IC₅₀ values reported. The Folin- Ciocalteu assay measured total phenolic content and was reported as mg gallic acid equivalents per gram of extract (mg GAE/g). Flavonoids per gram of extract were reported in mg quercetin equivalents (mg QE/g), measured using the AlCl₃ colourimetric technique. The data were analysed statistically through one-way ANOVA, followed by Duncan’s Multiple Range Test, with a significance level of p < 0.05. The fresh control produced a wet-basis extract yield of 0.82%, whereas the dried samples produced dry-basis yields of 3.0%, 3.2%, and 5.1% at 40, 50, and 60 °C, respectively. The highest antioxidant activity occurred in the 40°C group, with the lowest IC₅₀ value of 17.83 ppm. The total phenolic content peaked at 60°C, indicating a value of 124.0 ± 1.59 mg GAE/g extract. TFC differed significantly across the four sample groups because the fresh control was lower than all dried groups; however, the 40, 50, and 60°C drying treatments did not differ significantly from one another.
BIOLOGY LABORATORY MANAGEMENT IN SENIOR HIGH SCHOOLS : AN ANALYSIS OF PLANNING, IMPLEMENTATION, AND RESOURCE UTILIZATION Much Fuad Saifuddin; Tegar Trianggoro Putro; Bekti Isnaeni
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 2 (2026): October 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i2.3906

Abstract

This descriptive qualitative study analysed biology laboratory management across two senior high schools in Gamping District during the 2018/2019 academic year. Informants selected through purposive sampling comprised two heads of laboratory, one laboratory technician, and two biology teachers, alongside descriptive questionnaire data from 627 students across 21 study groups. Data collected via semi-structured interviews, direct observations, document reviews, and questionnaires were analysed using Miles and Huberman’s qualitative framework (comprising data reduction, data display, and conclusion drawing). Results revealed three principal findings: First, operational capacity diverged sharply, as High School A maintained routine practical sessions using a dedicated technician, whereas High School B faced physical constraints, technician shortages, and multi-purpose room usage. Second, field observations and student surveys identified persistent sanitation infrastructure deficits across both institutions, including irregular running water access and unsegregated chemical waste. Third, both schools exhibited an administrative omission in evaluative governance, maintaining basic activity logs while completely omitting formal staff performance appraisals and post-evaluation action plans. To ensure sustainable laboratory operations, institutional management must prioritise technical staffing, sanitation infrastructure upgrades, and formalised performance evaluation frameworks.
IDENTIFICATION OF STUDENTS' MISCONCEPTIONS USING THE CERTAINTY OF RESPONSE INDEX (CRI) METHOD ON MONOHYBRID CROSSING MATERIAL Anita Anita; Fenny Roshayanti; Endah Rita Sulistya Dewi; Siti Patonah
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 2 (2026): October 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i2.4185

Abstract

This study aims to identify students' conceptual understanding and analyze indications of misconceptions in monohybrid cross material using the Certainty of Response Index (CRI) method. The study was conducted at SMP Negeri 2 Singorojo with 30 ninth-grade students as research subjects. Data were collected using a diagnostic test accompanied by a Certainty of Response Index (CRI) scale to identify students' confidence in their answers. The data were analyzed by classifying students' responses into four categories: Conceptual Understanding (PK), Conceptual Understanding with Less Confidence (PKKY), Misconceptions (M), and Conceptual Uncertainty/Do Not Know the Concept (TTK). The results showed that 40% of the analyzed responses were classified as Conceptual Understanding, 17% as Conceptual Understanding with Less Confidence, 37% as Misconceptions, and 6% as Conceptual Uncertainty/Do Not Know the Concept. The highest proportions of suspected misconceptions were identified in the sub-concepts of basic genetics, particularly genotype and phenotype, gamete formation and segregation, and determining cross results using Punnett squares. These findings indicate that students experienced substantial conceptual difficulties in connecting genetic representations, allele segregation, and the probability of offspring inheritance. This provides an empirical basis for designing subsequent instructional interventions that emphasize conceptual visualization, genetic processes, and contextual learning to strengthen students' understanding of monohybrid crosses.
EFFECTS OF ETHANOLIC GARLIC (Allium sativum) AND BITTER MELON (Momordica charantia) EXTRACTS ON SERUM CHOLESTEROL AND MALONDIALDEHYDE IN HIGH-FAT-DIET RATS Ayuk Karisna; Noer Kumala Indahsari; Rini Purbowati
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 2 (2026): October 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i2.4259

Abstract

This study compared the effects of ethanolic garlic (Allium sativum) and bitter melon (Momordica charantia) on total cholesterol and serum MDA in high-fat-diet rats. Thirty-five male Rattus norvegicus were randomized into seven groups (n=5): standard-diet control, untreated high-fat-diet control, simvastatin comparator, garlic extract at 300 or 600 mg/kg body weight, and bitter melon extract at 300 or 600 mg/kg body weight. Following 14 days of high-fat-diet induction, treatments were administered orally for 14 days. Total cholesterol levels were measured before and after the intervention (pre-test and post-test), while serum MDA was measured by the thiobarbituric acid-reactive substances method. Nonparametric analyses used Kruskal–Wallis tests followed by pairwise comparisons with Bonferroni adjustment for multiple comparisons. Post-test cholesterol differed among groups (H=15.396; p=0.017), as did cholesterol change (H=15.208; p=0.019); however, the untreated high-fat-diet group had lower post-test cholesterol than the standard-diet and simvastatin groups 40 mg/kg body weight, and none of the extract groups differed significantly from the untreated high-fat-diet group. By contrast, MDA differed markedly (H=21.692; p=0.001). Garlic at 300 and 600 mg/kg and bitter melon at 300 and 600 mg/kg significantly lowered MDA relative to the untreated high-fat-diet group, with the lowest mean in the bitter melon 600 mg/kg group (2.72±1.52 nmol/mL). Both extracts demonstrated antioxidant activity, but the cholesterol findings require verification against the raw laboratory records before firm hypolipidemic conclusions are drawn.

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