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Bionature
Published by Universitas Negeri Makassar
ISSN : 14114720     EISSN : 26545160     DOI : 10.35580
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Bionature is a journal that contains writings based on the results of research, conceptual ideas, studies, and the implementation of the theory in the field of biology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 6 Documents
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Search results for , issue "Vol. 25 No. 1 (2024): April-Juni 2024" : 6 Documents clear
Antibiotic Sensitivity Testing of Bacteria Isolated from Patients Suspected of Urinary Tract Infection: Uji Sensitivitas Antibiotik terhadap Bakteri yang Diisolasi dari Pasien Diduga Infeksi Saluran Kemih Erpi Nurdin; Suanbani, Dawika; Mukhtasyam Zuchrullah
Bionature Vol. 25 No. 1 (2024): April-Juni 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i1.1883

Abstract

Urinary tract infections (UTIs) is a condition wherein inflammation occurs, caused by the proliferation of microorganisms in the human urinary tract. Antibiotics are the main choice in treating UTIs, the aim of giving antibiotics is to treat acute infections and prevent urosepsis. However, deviant use of antibiotics will reduce the sensitivity of antibiotics to bacteria. The aim of this study was to determine the sensitivity of bacteria to antibiotics from urine specimens of patients with suspected urinary tract infections (UTIs). The examination begins with the determination of the bacterial species and continues with the sensitivity test of the Kirby-Bauer diffusion method, looking at the barrier zone of each antibiotic. The observation results showed that there were 6 types of bacteria found from the urine culture results of patients with suspected urinary tract infections, with the most types of bacteria being gram positive S. aureus in 13 cases (40%), gram negative E. coli in 6 cases (18%), K. oxytoca and E. aerogenes were 5 cases (15%). According to the results of the antibiotic sensitivity test, each one shows the percentage of sensitive antibiotics. Ciprofloxacin is 100% against E. coli, K. oxytoca, P. mirabilis, 60% against E. aerogenes and 46% against S. aureus. Gentamicin is 100% against E. coli, K. oxytoca, P. mirabilis. Ampicillin is 83% against E. coli, 60% against S. epidermidis. Amoxicillin is 66% against E. coli. In conclusion, based on test results, Ciprofloxacin has better sensitivity, followed by gentamicin, ampicillin and amoxicillin. Keywords: antibiotics, sensitivity testing, urinary tract infection
Application of Manure on Alluvial Soil to The Changes of Soil Chemical Properties Andrayani, Ketti; Sangkala, Sangkala; Susilawati, Susilawati
Bionature Vol. 25 No. 1 (2024): April-Juni 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i1.1928

Abstract

The objective of the study is to test the chemical content of alluvial soil with the application of manure. Alluvial soil has poor physical and chemical soil properties, including low pH, nutrients, C-organic soil cation exchange capacity and a low C/N ratio. Therefore, it requires action to improve the planting by adding manure ameliorant. This research was carried out from February 5th, 2023, to April 5th, 2023 in Sambas Regency, Sambas District. This research uses a descriptive analysis model by comparing laboratory analyses using samples before and after the incubation process for 30 days. The treatments referred to in this research are: chicken manure, cow manure and goat manure. The dose of alluvial soil and manure used is 1:1. The parameters used in the research include soil pH, soil CEC, organic carbon (C), C/N ratio, elements nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg). The results of the research showed that there were changes in the chemical properties of the soil after being treated using chicken manure, cow manure and goat manure when compared to the untreated soil. The use of chicken manure on alluvial soil has a more beneficial effect on changes in soil pH values, soil CEC, C-organics, C/N ratio, N, P, K, Ca, and Mg elements when compared with the use of cow manure.
The Effects of Andrographis Paniculata (Burm. f.) Nees Extract on the Liver White Rats (Rattus norvegicus L.) induced by Lead Acetate Andrian, Luthfiyah; Febriani, Husnarika; Tambunan, Efrida Pima
Bionature Vol. 25 No. 1 (2024): April-Juni 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i1.2061

Abstract

Lead acetate is classified as a heavy metal compound that has the ability to stimulate the overproduction of free radicals, sometimes referred to as Reactive Oxygen Species (ROS), leading to oxidative stress at the cellular level. When lead acetate is introduced into the body, it undergoes systemic circulation and distributes to several organs, including the liver. Prolonged exposure of the liver to high levels of lead can result in hepatic damage. The leaves of Andrographis paniculata (Burm. fil.) Ness are known to possess antioxidant properties, rendering them suitable for medicinal use in combating free radicals. The objective of this study is to investigate the impact of ethanol extract derived from Andrographis paniculata (Burm. fil.) Ness on the morphological characteristics and hepatic functionality of white rats (Rattus norvegicus L.) that have been treated with lead acetate. The present study employed a completely randomized design, consisting of five treatment groups and five repetitions. The control group received only food and drink, while the experimental group was administered lead acetate for a duration of 28 days at a dosage of 40 mg. The treatment groups, on the other hand, were each administered ethanol extract of Sambiloto leaves at dosages of 250, 500, and 750 mg/kg BW for a period of 14 days. The findings indicated that the treatment of lead acetate resulted in liver histological damage, specifically parenchymatous degeneration, hydropic degeneration, and necrosis. Additionally, it led to elevated levels of SGPT and SGOT. The study's findings indicate that the administration of a 750 mg/kg BW dose of ethanol extract derived from Sambiloto leaves can effectively mitigate liver histological damage and lower levels of SGPT and SGOT.
Ethnobotanical Studies Medicinal Plants in Taulan Village, Regency Enrekang Syamsiah, Syamsiah; Aulya, Rezky; Hiola, St. Fatmah; Abd. Muis
Bionature Vol. 25 No. 1 (2024): April-Juni 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i1.2199

Abstract

Ethnobotany is science that studies about utilization plant in a way traditional, one of them that is plant drugs. This study aims to determine types plant medicine and methods its use by village communities Taulan, Regency Enrekang. This research is of a nature descriptive explorative with method survey as well as technique interview to give detailed description of utilization plant medicine by the people in the village Taulan, Regency Enrekang. Method used is snowball sampling. Interview process done in an open-ended manner with guided by the table interview. Research result show as many as 53 types plants used by village communities Taulan as plant drug. Plant organs used​ like rhizomes, roots, stems, leaves, fruit, and flowers. Processing plant drug done with method simple, that is with method boiled, squeezed, chewed, or eaten direct as fresh vegetables.
The Effect of Synthetic Fertilizer Urea, TSP and ZA on Total Biomass and Chlorophyl Content of Dunaliella sp. Junda, Muhammad; Adhyani, Andi Nurul; Muis, Abd; Kurnia, Nani; Rachmawaty, Rachmawaty
Bionature Vol. 25 No. 1 (2024): April-Juni 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i1.2229

Abstract

This research is an experimental research which aims to determine the effect of synthetic fertilizers urea, TSP and ZA on the amount of biomass and chlorophyll content of Dunaliella sp. on a laboratory scale. The research design used was a completely randomized design (CRD) consisting of 5 treatments with 3 replications each to obtain 15 treatment combinations. The research method consisted of several stages, namely preparation of tools and materials consisting of making culture media, making walne medium and making treatment medium, sterilizing tools and medium, preparing Dunaliella sp inoculum. on Walne medium with a static system, as well as optimizing the growth of Dunaliella sp.. The parameters observed in this study were phytoplankton biomass (dry weight), total chlorophyll content, salinity, pH, temperature, and light intensity. Based on the research results, the highest average biomass was found in the P3 treatment, namely the Walne medium treatment with the addition of 105 ppm urea, 80 ppm TSP, and 80 ppm ZA which produced an average of 5.23 g/L. Furthermore, the highest average results for measuring chlorophyll content were found in treatment P4 with walne medium treatment which added 115 ppm urea, 75 ppm TSP and 75 ppm ZA which produced an average of 1.81 µg/mL. So it can be concluded that the synthetic fertilizers urea, TSP and ZA have an effect on the amount of biomass and chlorophyll content on the growth of Dunaliella sp.
Optimization of Pectin Extraction from Pomelo Peels (Citrus maxima) using Response Surface Methodology Pagarra, Halifah; Hartati; Rachmawaty, Rachmawaty; Sahribulan, Sahribulan
Bionature Vol. 25 No. 1 (2024): April-Juni 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i1.2340

Abstract

Pectin is an essential polysaccharide that is usually found in the skin of various fruits and vegetables. It acts as a gelling agent and stabilizer in many food and pharmaceutical industries. This study focuses on the potential of grapefruit peel as a source of pectin. Pectin is isolated from hot grapefruit peel powder (albedo). The aim of this study was to extract pectin from grapefruit peel (albedo), and central composite design (CCD) together with the numerical optimization method of response surface methodology (RSM) was used to optimize the processing parameters, including temperature (60, 80 and 100 ° C), pH (1.5, 2.0 and 2.5 ) and time (60, 90 and 120 min) The findings show that the studied parameters have a major impact on the pectin yield. The resulting second-order polynomial model thoroughly explains the relationship between input and output variables and how the data is varied. The highest pectin yield was 28.45% at optimal conditions achieved: temperature: 100° C; pH: 1.5; and extraction time: 120 minutes. Temperature, pH, and extraction time have their own large impact on pectin yield which has a significant effect.

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