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INDONESIA
Jurnal Penelitian Saintek
Published by Universitas Negeri Yogyakarta
ISSN : 14123991     EISSN : 25287036     DOI : 10.21831
Core Subject : Science,
Decision Sciences, Operations Research & Management
Arjuna Subject : -
Articles 286 Documents
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Analisis kebutuhan ruang dan penataan parkir kendaraan mobil Adi Mawardin; Dinda Fardila; Sari Yusira
Jurnal Penelitian Saintek Vol 27, No 1 (2022)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.48070

Abstract

Empire XXI merupakan salah satu tempat hiburan yang ada di kota Yogyakarta. Jumlah kendaraan mobil yang datang ke Empire XXI untuk hari-hari tertentu sangat ramai hal ini justru tidak seimbang dengan kapasitas ruang parkir yang ada hingga sering terjadi penumpukkan kendaraan mobil di pintu masuk yang menunggu. Parkir adalah keadaan tidak bergerak suatu kendaraan yang bersifat sementara karena ditinggal oleh pengemudinya. Tujuan dari penelitian ini adalah untuk karakteristik parkir kendaraan mobil pada lokasi studi yaitu Empire XXI Yogyakarta berupa: akumulasi parkir, volume, konfigurasi parkir, turnover, indeks parkir, headway dan durasi parkir dan memberikan alternatif ruang parkir di area Empire XXI Yogyakarta. Analisis menunjukkan bahwa luas 915,4 m2 mampu menampung 56 mobil sedangkan data primer yang ada 1.397,25 m2 dengan daya tampung kapasitas 90 kendaraan mobil. Saran kepada pihak Empire XXI Yogyakarta agar dilakukan perluasan lahan menggunakan tanah di samping Empire sebanyak 777 m2 sehingga mampu menambah kapasitas sebanyak 50 dengan menggunakan sudut 90o dan membuat pos jaga barusehingga dapat memisahkan pos masuk motor dan mobil.Analysis of space requirements and car parking arrangementsEmpire XXI is one of the entertainment venues in Yogyakarta city. The number of car vehicles that come to Empire XXI for certain days is very crowded this is actually not balanced with the capacity of existing parking spaces until there is often a accumulation of car vehicles at the entrance that waits. Parking is a state of motionless a vehicle that is temporary because it is left by the driver. The purpose of this study is for the characteristics of car parking at the study site, namely Empire XXI Yogyakarta in the form of: parking accumulation, volume, parking configuration, turnover, parking index, headway and parking duration and provide an alternative parking space in the Empire XXI Yogyakarta area. The analysis showed that an area of 915.4 m2 was able to accommodate 56 cars while the existing primary data was 1,397.25 m2 with a capacity capacity of 90 car vehicles. Advice to Empire XXI Yogyakarta to expand land using land beside Empire as much as 777 m2 so that it can increase capacity as much as 50 by using a 90o angle and create a new guard post, so that it can separate motor and car entry posts.
Peningkatan kualitas air Waduk Diponegoro Semarang dengan filter arang aktif Nia Fadlilatul Laily; Nurhayati Nurhayati; Endang Kusdiyantini; Arina Tri Lunggani
Jurnal Penelitian Saintek Vol 27, No 1 (2022)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.48195

Abstract

Air bersih harus memiliki kualitas yang baik dari segi fisika, kimia, maupun biologisnya. Waduk Diponegoro mempunyai peran strategis dalam pengelolaan sumber daya air Kota Semarang. Berdasarkan hasil survei pada Waduk Diponegoro. Tujuan penelitian untuk meningkatkan kualitas air Waduk Diponegoro berdasarkan kategori kelas indeks kualitas air parameter biologi, fisika, dan kimia dengan perlakuan filter arang aktif. Arang aktif banyak digunakan untuk menghilangkan kontaminan dalam air karena kapasitasnya yang tinggi untuk adsorpsi senyawa tersebut, yang timbul dari luas permukaan dan porositasnya yang besar serta dapat digunakan untuk mengurangi keberadaan E. coli pada air. Proses uji bakteriologis air dilakukan dengan menggunakan metode deteksi bakteri dengan 3M Petrifilm E.coli/Coliform. Metode yang digunakan pada penelitian meliputi sterilisasi alat, pengambilan sampel air pada titik inlet dan outlet, filtrasi air dengan ketebalan arang aktif 10, 15, dan 20 cm, inokulasi sampel air, perhitungan jumlah koloni, pengukuran dan penentuan kualitas air. Hasil penelitian menunjukkan bahwa semakin tebal media arang aktif yang digunakan semakin baik kualitas air yang dihasilkan. Perlakuan filtrasi arang aktif dapat meningkatkan kualitas air Waduk Diponegoro Semarang dari kelas D kategori tercemar berat menjadi kelas C kategori tercemar sedang berdasarkan parameter biologi, fisika, dan kimia.Improve the water quality of Diponegoro Reservoir with active charcoal filterClean water must have good quality in terms of physics, chemistry, and biology. Diponegoro Reservoir has a strategic role in the management of Semarang City’s water resources. Based on the results of the survey on Diponegoro Reservoir. The purpose of the study is to improve the water quality of Diponegoro Reservoir based on the class category of water quality index of biological, physical, and chemical parameters with active charcoal filter treatment. Activated charcoal is widely used to remove contaminants in water due to its high capacity for adsorption of the compound, which arises from its large surface area and porosity and can be used to reduce the presence of E. coli in water. The water bacteriological test process is carried out using a method of detection of bacteria with 3M Petrifilm E.coli/Coliform. Methods used in the study include sterilization of tools, sampling of water at inlet and outlet points, filtration of water with the active charcoal thickness of 10, 15, and 20 cm, inoculation of water samples, calculation of the number of colonies, measurement and determination of water quality. The results showed that the thicker the active charcoal media used the better the quality of the water produced. Active charcoal filtration treatment can improve the water quality of Diponegoro Reservoir Semarang from class D category heavily polluted to class C moderately polluted category based on biological, physical, and chemical parameters.
Uji dan identifikasi aktivitas antioksidan isolat BAL CIN-2 hasil isolasi cincalok Ihdina Isfara Suteja; Wijanarka Wijanarka; Endang Kusdiyantini
Jurnal Penelitian Saintek Vol 27, No 1 (2022)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.44187

Abstract

Penelitian ini bertujuan untuk menguji antioksidan yang dihasilkan oleh isolat BAL CIN-2 dengan metode (α,α-diphenyl-β-picrylhydrazyl) (DPPH) dan fosfomolibdenum, melakukan identifikasi senyawa antioksidan yang dihasilkan isolat BAL CIN-2 menggunakan metode TLC (Thin Layer Chromatography) dan spektrofotometer UV-Vis, serta menganalisis gugus fungsional antioksidan isolat BAL CIN-2 menggunakan metode Fourier Transform Infrared Spectroscopy (FTIR). Kultur ditumbuhkan pada media MRSA dan diamati pertumbuhannya pada media MRSB selama 72 jam, selanjutnya dianalisis dengan metode yang telah ditentukan. Hasil uji TLC untuk isolat BAL CIN-2 menghasilkan empat noda dengan nilai Rf 0,98; 0,91; 0,73; dan 0,6. Uji DPPH menunjukkan sifat antioksidan supernatan isolat BAL CIN-2 sangat lemah. Metode fosfomolibdenum menunjukkan kadar antioksidan dari supernatan isolat BAL CIN-2 sebesar 1,906 mgAAE/ml. Pengujian menggunakan spektrofotometer UV-Vis menghasilkan 4 serapan gelombang dengan absorbansi maksimum 3,943 pada panjang gelombang 290 nm. Diduga senyawa yang terkandung di dalamnya adalah senyawa flavonoid golongan flavanon atau dihidroflavonol. Supernatan isolat BAL CIN-2 diduga mengandung senyawa flavonoid dengan gugus fungsi antioksidan O-H dan C=C aromatik dari analisis menggunakan FTIR. Berdasarkan hasil tersebut dapat disimpulkan bahwa di dalam supernatan isolat BAL CIN-2 mengandung senyawa antioksidan golongan flavonoid.Test and identification of antioxidant activity of isolates LAB CIN-2 isolated from cincalokThis study aimed to test the antioxidants produced by LAB CIN-2 isolates using α,α-diphenyl-β-picrylhydrazyl (DPPH) and phosphomolybdenum methods, to identify antioxidant compounds produced by LAB CIN-2 isolates using the Thin Layer Chromatography (TLC) and UV-Vis spectrophotometer, as well as to analyse the antioxidant functional groups of LAB CIN-2 isolate using the Fourier Transform Infrared Spectroscopy (FTIR) method. The culture was grown on MRSA media and then the growth was observed on MRSB media for 72 hours, then analyzed using the specified method. TLC test results showed that LAB isolate CIN-2 produced four spots with an Rf value of 0.98; 0.91; 0.73; and 0.6. The DPPH test showed that the antioxidant properties of the supernatant isolates of LAB CIN-2 were very weak. The phosphomolybdenum method showed that the antioxidant content of the supernatant of LAB CIN-2 isolate was 1.906 mgAAE/ml. Analysis using a UV-Vis spectrophotometer produces 4 absorption waves with a maximum absorbance 3,943 at a wavelength of 290 nm. The test is suspected that the compounds contained in it are flavonoid compounds of the flavanone or dihydroflavonol group. Analysis using FTIR showed that antioxidant from supernatant of LAB isolate CIN-2 was suspected to contain flavonoid compounds with aromatic O-H and C=C functional groups. It can be concluded that supernatant from LAB CIN-2 isolates contains weak antioxidants in the form of compounds from the flavonoids group.
Energy profile and improvement proposal of a sawdust charcoal briquette factory Akmal Irfan Majid; Syafik Maulana; Willie Prasidha
Jurnal Penelitian Saintek Vol 27, No 1 (2022)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.48513

Abstract

This study aimed to analyze the profile of energy consumption and identify efforts to improve energy efficiency. In the present work, the evaluation was focused on the main production lines, including energy utilities sourced from electricity as well as an analysis of thermal energy-based equipment. A medium-scale charcoal plant in Yogyakarta was selected as the object of the present study to represent the characteristics of a sawdust charcoal briquette industry. Measurements of electrical current were made using a clamp meter to determine the utility of the electrical devices while an infrared thermal camera was used to determine the temperature distribution. Results of this study indicate that thermal energy has the largest proportion in the main production line. Some points of heat concentration were found which could potentially cause hazards and thermal discomfort. Ideas for improvement were also proposed qualitatively as a recommendation
Simulasi optimalisasi kapasitas pembangkit listrik tenaga surya pada atap gedung Samsurizal Samsurizal; Andi Makkulau; Rio Afrianda
Jurnal Penelitian Saintek Vol 27, No 1 (2022)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.44461

Abstract

DKI Jakarta merupakan salah satu daerah perkotaan yang padat penduduk dan memiliki bangunan yang padat sehingga pemanfaatan atap bangunan dapat digunakan sebagai sumber energi. Salah satu gedung yang dapat menerapkan pemanfaatan energi terbarukan yaitu gedung administrasi di PT. PJB Unit Pembangkit Muara Karang. Pada gedung tersebut telah dibangun, namun pemasangannya belum optimal dikarenakan luasan pemasangan modul surya kecil sehingga kapasitas yang dihasilkan kecil pula, sementara potensi lokasi yang ada sangat luas. Oleh karena itu, perlu dikaji sejauh mana optimalisasi kapasitas pembangkit listrik tenaga surya pada atap gedung. Dari penelitian yang sudah dilakukan didapat hasil perlu dipasang modul surya sejumlah 143 modul panel surya, dengan kapasitas yang dihasilkan saat terpasang sebesar 42.900 wp dan didapatkan pula potensi energi yang dihasilkan sebesar 93.951 kWh/tahun atau sama dengan mampu menyuplai kebutuhan energi pada di gedung tersebut sebesar 75,11% dan rata-rata produksi harian sebesar 36,27 kWh. Agar potensi energi yang dihasilkan maksimal, perlu optimalisasi potensi dan besarnya luas atap pada gedung. Energi yang dihasilkan dapat semaksimal mungkin di manfaatkan sebagai energi listrik yang pada akhirnya dapat mengurangi beban biaya pemakaian energi listrik.Optimization of solar power plant capacity on the roof of the buildingDKI Jakarta is one of the densely populated urban areas and has dense buildings so that the use of building roofs can be used as a source of energy. One of the buildings that can implement the utilization of renewable energy is the administration building in PT. PJB Muara Karang Power Plant Unit. In the building has been built, but the installation is not optimal because the installation area of small solar modules so that the resulting capacity is small, while the potential of the existing location is very wide. Therefore, it is necessary to examine the extent of optimization of solar power plant capacity on the roof of the building. From the research that has been done obtained the results of the need to install solar modules a number of 143 solar panel modules, with the capacity produced when installed by 42,900 wp and also obtained the potential of energy produced by 93,951 kWh / year, or the same as being able to supply energy needs in the building by 75.11 % and average daily production of 36.27 kWh. In order for the maximum energy potential to be optimized the potential and the size of the roof area in the building. The energy produced can be utilized as electricity that can ultimately reduce the cost of electricity consumption.
PEMANFAATAN AMPAS DAUN TEH PADA PROSES BIOSORPSI LOGAM BERAT Cr(VI) PADA AIR SUNGAI CITARUM Suci Rizki Nurul Aeni; Farhan Baehaki; Syifa Zayna Muwahiddah
Jurnal Penelitian Saintek Vol 27, No 2 (2022)
Publisher : Directorate of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v2i27.52841

Abstract

Limbah Cr(VI) dapat mengganggu kesehatan karena bersifat toksik, maka perlu dilakukan pengolahan air sungai terlebih dahulu. Salah satu Teknik pengolahan alternatif yaitu menggunakan metode biosorpsi dengan biosorben ampas daun teh. Namun hasil penyerapan itu tergantung oleh beberapa faktor salah satunya yaitu waktu kontak. Tujuan penelitian ini untuk mengetahui waktu kontak optimum terhadap proses biosorpsi Cr(VI) dengan menggunakan biosorben ampas daun teh. Penelitian ini menggunakan metode deskriptif kuantitatif. Sampel yang digunakan air Sungai Citarum yang diambil dari empat titik yaitu di Desa Pangauban, Desa Cilampeni, Desa Nanjung dan Desa Lagadar. Pengukuran kadar dilakukan menggunakan Spektrofotometer UV-Vis pada panjang gelombang 540 nm. Berdasarkan penelitian ini diperoleh waktu kontak optimum yaitu 45 menit dengan presentase penurunan kadar dari masing-masing sampel yaitu 73,25% (Desa Pangauban), 75,94% (Desa Cilampeni), 71,88%, (Desa Nanjung) dan 74,31% (Desa Lagadar). Oleh karena itu, waktu kontak merupakan faktor yang perlu diperhatikan dalam proses biosorpsi untuk pengolahan limbah logam berat Cr(VI). Pemanfaatan ampas daun teh pada proses biosorpsi logam berat Cr(VI) pada air sungai CitarumCr(VI) waste can interfere with health because it is toxic, so it is necessary to treat river water first. One alternative treatment is using the biosorption method with tea leaf dregs biosorbent. However, the absorption results depend on several factors, one of which is contact time. The purpose of this study was to determine the optimum contact time for the biosorption process of Cr(VI) using tea leaf dregs biosorbent. This study used a quantitative descriptive method. The samples used were Citarum River water taken from four points, namely in Pangauban Village, Cilampeni Village, Nanjung Village and Lagadar Village. The concentration measurement was carried out using a UV-Vis Spectrophotometer at a wavelength of 540 nm. Based on this study, the optimum contact time was 45 minutes with the percentage reduction in levels of each sample, namely 73.25% (Pangauban Village), 75.94% (Cilapeni Village), 71.88%, (Nanjung Village) and 74, 31% (Lagadar Village). Therefore, contact time is a factor that needs to be considered in the biosorption process for the treatment of Cr(VI) heavy metal waste
Identifikasi Jamur Trichophyton sp. Penyebab Tinea Unguium Pada Petani Desa Mekarluyu Kabupaten Garut Firdha Rachmawati; Perdina Nursidika; Putri Fitrianingsih
Jurnal Penelitian Saintek Vol 27, No 2 (2022)
Publisher : Directorate of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v2i27.52528

Abstract

Infeksi jamur menyerang lebih dari 6 juta penduduk Indonesia setiap tahunnya. Tinea unguium merupakan infeksi mikosis superfisialis dengan insidensi 30% dan merupakan penyebab 50% kelainan kuku. Desa Mekarluyu Kabupaten Garut merupakan daerah dengan mayoritas penduduk bekerja sebagai petani. Keadaan lingkungan kerja yang basah, lembab dan suhu yang hangat meningkatkan risiko penyakit Tinea unguium pada petani. Tinea unguium disebabkan oleh jamur dermatofita dengan penyebab utama yaitu jamur Trichophyton sp. Oleh karena itu, penelitian ini bertujuan untuk mengidentifikasi Trichophyton sp. penyebab tinea unguium pada kuku kaki petani Desa Mekarluyu Kabupaten Garut. Penelitian ini menggunakan metode cross-sectional dengan responden sebanyak 30 orang yang diketahui menunjukkan gejala Tinea unguium. Sampel kerokan kuku yang telah diambil kemudian ditumbuhkan pada media Saboraud Dextrose agar untuk diamati koloninya. Pengamatan mikroskopis hasil biakan dilakukan dengan pewarnaan Lactophenol Cotton Blue. Hasil penelitian didapatkan sebanyak 23% responden terinfeksi oleh Trichophyton sp. dengan spesies yang ditemukan yaitu Trichophyton rubrum 17% dan Trichophyton mentagrophytes (6%).Identification of fungi causing Tinea Unguium Trichophyton sp. on farmers in Mekarluyu Village Garut RegencyMore than 6 million Indonesian people were infected by fungi every year. Tinea unguium is a superficial mycosis infection with an incidence 30% and 50% main causes of nail disorders. Mekarluyu Village, Garut Regency is an area with the majority of the population working as farmers. Wet, humid and warm temperatures working conditions increase the risk of tinea unguium in farmers. Main aetiology of tinea unguium is Trichophyton sp. Therefore, this study aims to identify Trichophyton sp. the cause of tinea unguium on the toenails of farmers in Mekarluyu Village, Garut Regency. This study used a cross-sectional method with 30 respondents who were known to show symptoms of tinea unguium. The nail scraping samples were grown on Saboraud Dextrose Agar media to observe the colonies. Microscopic observations of the colonies were carried out with Lactophenol Cotton Blue staining. The results showed that 23% of respondents were infected by Trichophyton sp. with the species Trichophyton rubrum 17% and Trichophyton mentagrophytes (6%).
ANTIBACTERIAL ACTIVITIES OF POLAR FRACTION OF PAPAYA LEAF ETHANOLIC EXTRACT (Carica papaya Linn.) AGAINST Escherichia coli AND Staphylococcus aureus Margaretha Kurniasari; Kurnia Rahayu Purnomo Sari; Nur'aini Purnamaningsih
Jurnal Penelitian Saintek Vol 27, No 2 (2022)
Publisher : Directorate of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v2i27.53006

Abstract

Tujuan penelitian untuk mengetahui aktivitas antibakteri fraksi polar ekstrak etanol daun pepaya (Carica papaya L.) terhadap bakteri Staphylococcus aureus dan Escherichia coli. Proses ekstraksi dilakukan menggunakan metode maserasi dengan pelarut etanol 70%. Ekstrak etanol selanjutnya difraksinasi menggunakan pelarut etanol-air. Uji aktivitas antibakteri yang digunakan adalah metode difusi agar cara Kirby Bauer. Variasi konsentrasi fraksi etanol-air ekstrak etanol daun pepaya 10, 15, 20, 25, 30, dan 100%. Zona hambat pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli diukur menggunakan jangka sorong. Hasil penelitian menunjukkan zona hambat fraksi etanol-air terhadap bakteri Staphylococcus aureus yang paling optimum yaitu pada konsentrasi 100% sebesar 14,75 mm; sedangkan pada bakteri Escherichia coli yang paling optimum yaitu pada konsentrasi 100% sebesar 11,53 mm. Namun tidak efektif jika dibandingkan dengan zona hambat kontrol positif kloramfenikol. Kesimpulan fraksi etanol-air ekstrak etanol daun pepaya (Carica papaya L.) dapat menghambat pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli.Antibacterial activities of polar fraction of papaya leaf ethanolic extract (Carica papaya Linn.) against Escherichia coli and Staphylococcus aureusThis study aimed to determine the antibacterial activities polar fraction of papaya leaf ethanol extract against Escherichia coli and Staphylococcus aureus. Simplicia was extracted by ethanol 70% with the maceration method. Ethanolic extract was fractionated with ethanol water. Kirby Bauer diffusion method agar was used for antibacterial activities. Variation concentration of the ethanol-water fraction of papaya leaf ethanolic extract 10, 15, 20, 25, 30, and 100%. The growth inhibition zones of Escherichia coli and Staphylococcus aureus are measured using a caliper. The zone of inhibition of the ethanol-water fraction against Staphylococcus aureus was the most optimum at a concentration of 100% at 14.75 mm, while the most optimum for Escherichia coli was at a concentration of 100% at 11.53 mm, but did not effective than positive control Chloramphenicol. The conclusion of this study is that the ethanol-water fraction of papaya leaf ethanol extract could inhibit the growth of Staphylococcus aureus and Escherichia coli bacteria.
Analysis of CVT (continuously variable transmission) and the influence of variations on the motorcycle Rinasa Agistya Anugrah
Jurnal Penelitian Saintek Vol 27, No 2 (2022)
Publisher : Directorate of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v2i27.53582

Abstract

This study was aimed to determine the working principle, troubleshooting, and the influence of CVT roller weight variations on Suzuki Nex-FI 2014 motorcycles. This research was conducted by analyzing and comparing the measurement results for each CVT component. This study used an experimental research by holding the throttle at 3,250 rpm. Once steady state is reached, the throttle is rotated up to 10,000 rpm. The results of the troubleshooting analysis and measurement of drive belt wear were from the standard size of 19.50 mm to 18.00 mm. The usage tolerance limit is 18.60 mm. As for the results of the roller variation test using a lighter weight namely 8 and 9 grams. The results show that the maximum results are achieved at low and medium rotational speeds, compared to the use of 11 gr (standard) roller. The weakness of the lighter roller (8 9 grams) is that they only get good acceleration and torque at low revs while at high rotation speed is less than the maximum. The use of standard (11 gram) roller has less maximum acceleration and torque but produces maximum power.
IDENTIFIKASI Escherichia coli PADA AIR MINUM ISI ULANG Nunung Sulistyani; Vera Millania
Jurnal Penelitian Saintek Vol 27, No 2 (2022)
Publisher : Directorate of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v2i27.52224

Abstract

Air memiliki peran penting bagi setiap makhluk hidup untuk memenuhi kebutuhan esensial. Asupan cairan dalam hal ini air minum menjadi sangat penting bagi tubuh manusia, maka perlu diperhatikan kualitas air minum sesuai standar yang telah ditetapkan. Saat ini, banyak penyelenggara air minum isi ulang mendapatkan ijin operasi untuk memproduksi air minum bagi masyarakat. Untuk menjaga kualitas air minum yang dikonsumsi masyarakat, dilakukan pengawasan secara berkala baik secara internal maupun eksternal. Penelitian ini bertujuan untuk mengidentifikasi adanya bakteri Escherichia coli pada salah satu merek air minum isi ulang yang berada di Yogyakarta yang bernama Khaira. Uji mikrobiologis dilakukan dalam penelitian ini. Data diperoleh melalui pemeriksaan air isi ulang menggunakan metode Most Probable Number (MPN). Hasil uji MPN menunjukkan tidak terdeteksi Escherichia coli pada semua sampel air. Kualitas air minum isi ulang yang diproduksi “Khaira” Yogyakarta berdasarkan uji identifikasi Escherichia coli memenuhi persyaratan batas cemaran mikroba.The identification of escherichia coli contains in ”Khaira” Yogyakarta refill waterWater has an important role for every living thing to meet essential needs. Fluid intake, in this case drinking water, is very important for the human body, so it is necessary to pay attention to the quality of drinking water according to established standards. Currently, many refill drinking water providers have obtained operating licenses to produce drinking water for the community. To maintain the quality of drinking water consumed by the community, periodic monitoring is carried out both internally and externally. This study was aimed to identify the Escherichia coli contains in one of the refill waters in Yogyakarta namely, Khaira. The microbiological test was used in this study. This research was conducted by identifying Escherichia coli in refill drinking water. The data was obtained by checking the refill water using the Most Probable Number (MPN) method. The MPN test results showed no Escherichia coli was detected in all water samples. The quality of refill drinking water produced by “Khaira” Yogyakarta based on the Escherichia coli identification test meets the requirements for microbial contamination limits.  

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